Conformational dynamics is important for enzyme catalysis. However, engineering dynamics to achieve a higher catalytic efficiency is still challenging. In this work, we develop a new strategy to improve the activity of yeast cytosine deaminase (yCD)...
The oxygen evolution reaction (OER) is a kinetic bottleneck in electrochemical water splitting, creating an urgent need for the development of efficient electrocatalysts. Prussian blue analogues (PBAs), a significant class of inorganic coordination p...
Two-dimensional-layered transition metal dichalcogenides (2D-layered TMDs) are a chemically diverse class of compounds having variable band gaps and remarkable electrochemical properties, which make them potential materials for applications in the fi...
Bacterial asparaginases (amidohydrolases, EC 3.5.1.1) are important enzymes in cancer therapy, especially for Acute Lymphoblastic Leukemia. They are tetrameric enzymes able to catalyze the deamination of L-ASN and, to a variable extent, of L-GLN, on...
Rechargeable zinc–air batteries (ZABs) are still impeded by the intrinsically sluggish kinetics of oxygen reduction and evolution reactions (ORR/OER) and by the instability or prohibitive price of state-of-the-art noble metal catalysts. Metal&n...
Dye-sensitized solar cells (DSSCs) have emerged as promising alternatives to traditional silicon-based solar cells due to their relatively high conversion efficiency, low cost, flexibility, and environmentally benign fabrication processes. In DSSCs,...
Facet engineering has emerged as a promising approach to tailor the catalytic performance of metal oxides for environmental electrocatalysis. Herein, we synthesized spinel Co3O4 nanocrystals with predominantly exposed {110}, {111}, and {112} facets t...
A two-dimensional (2D) polar monolayer with a polarization electric field can be used as a potential photocatalyst. In this work, first principle calculations were used to investigate the stability and photocatalytic properties of 2D polar monolayer...
Adipic acid is an important raw chemical for the commercial production of polyamides and polyesters. The traditional industrial adipic acid production utilizes nitric acid to oxidize KA oil (mixtures of cyclohexanone and cyclohexanol), leading to the...
Protein engineering is the design and modification of protein structures to optimize their functions or create novel functionalities for applications in biotechnology, medicine or industry. It represents an essential scientific solution for many of t...
Defective functional-group-endowed polymer semiconductors, which have unique photoelectric properties and rapid carrier separation properties, are an emerging type of high-performance photocatalyst for various energy and environmental applications. H...
Three UiO-66 samples were prepared by solvothermal synthesis using the defect engineering approach with benzoic acid as a modulator. They were characterized by different techniques and their acidic properties were assessed by FTIR spectroscopy of ads...
Laccase, one of the metalloproteins, belongs to the multicopper oxidase family. It oxidizes a wide range of substrates and generates water as a sole by-product. The engineering of laccase is important to broaden their industrial and environmental app...
The routine generation of enzymes with completely new active sites is a major unsolved problem in protein engineering. Advances in this field have thus far been modest, perhaps due, at least in part, to the widespread use of modern natural proteins a...
Constructing fast electron transfer pathways and abundant electro-active sites is an effective strategy to improve the oxygen evolution reaction (OER) performance of catalysts. Herein, structural engineering and dual-phase engineering were employed t...
Atomically dispersed catalysts, including single-atom, dual-atom, and periodic single-metal site catalysts, have revolutionized electrocatalysis by merging atomic precision with heterogeneous stability. This review traces their evolution from pioneer...
Molybdenum disulfide (MoS2) is a promising earth-abundant electrocatalyst for the hydrogen evolution reaction (HER), attributed to its favorable electronic structure and chemical stability. Nevertheless, its application is limited by poor electrical...
Human milk oligosaccharides (HMOs) signify a unique group of oligosaccharides in breast milk, which is of major importance for infant health and development. The functional benefits of HMOs create an enormous impetus for biosynthetic production of HM...
Project schedule monitoring and controlling are critical challenges of construction project management that are not adequately implemented, likely due to the predominance of earned value management and the lack of utilizing technology, such as BIM to...
A “thermal-annealing vacancy-filling” synthesis strategy was developed to engineer cobalt single-atom catalysts (Co-MoS2/RGO) for exceptional hydrogen evolution reaction (HER) performance. By anchoring atomic Co onto Frenkel defect-engine...
Modifying the electronic structure of a catalyst through interface engineering is an effective strategy to enhance its activity in the hydrogen evolution reaction (HER). Interface engineering is a viable strategy to enhance the catalytic activity of...
To improve the pharmacological properties of maize ribosome-inactivating protein (maize RIP) for targeting HIV-infected cells, the previously engineered TAT-fused active form of maize RIP (MOD) was further engineered for cysteine-directed PEGylation....
Leghemoglobin (LegH), a plant-derived hemoprotein, is engineered for the first time as a standalone biocatalyst for carbene N–H insertion. Through semi-rational design, the K65P mutation in the heme pocket significantly enhances catalytic effic...
Water, which is an intrinsic part of the exhaust gas of combustion engines, strongly inhibits the methane oxidation reaction over palladium oxide-based catalysts under lean conditions and leads to severe catalyst deactivation. In this combined experi...
Supercapacitors are a kind of energy storage device that lie between traditional capacitors and batteries, characterized by high power density, long cycle life, and rapid charging and discharging capabilities. The energy storage mechanism of supercap...
Interest in protein engineering for the enzymatic production of valuable products, such as pharmaceutical compounds and biofuels, is growing rapidly. The cold-active acetyl xylan esterase from Paenibacillus sp. (PbAcE) presents unusually broad substr...
The members of the Old Yellow Enzyme (OYE) family are capable of catalyzing the asymmetric reduction of (E/Z)-citral to (R)-citronellal—a key intermediate in the synthesis of L-menthol. The applications of OYE-mediated biotransformation are usu...
AppA, the Escherichia coli periplasmic phytase of clade 2 of the histidine phosphatase (HP2) family, has been well-characterized and successfully engineered for use as an animal feed supplement. AppA is a 1D-6-phytase and highly stereospecific but tr...
The problem of removing NOx and carbon particle emissions from diesel engines has been a challenge in the field of environmental protection, which is prompting people to actively explore ways to improve the efficiency of pollutant emission treatment....
Uridine diphosphate glycosyltransferases (UGTs) are known for promiscuity towards sugar acceptors, a valuable characteristic for host plants but not desirable for heterologous biosynthesis. UGTs characterized for the O-glycosylation of isoflavonoids...
The development of efficient, low-cost electrocatalysts for the oxygen evolution reaction (OER) is crucial for advancing sustainable hydrogen production through water splitting. This study presents a dual-engineering strategy to enhance the OER perfo...
Transition metal dichalcogenides (TMSs), exemplified by molybdenum disulfide (MoS2), exhibit significant potential as alternatives to noble metals (e.g., Pt) for the hydrogen evolution reaction (HER). However, conventional synthesis methods of MoSx o...
Significant interest has emerged for the application of Pd-In2O3 catalysts as high-performance catalysts for CO2 hydrogenation to CH3OH. However, precise active site control in these catalysts and understanding their reaction mechanisms remain major...
Carbon dioxide (CO2) emissions from the construction of road infrastructure have been of growing interest in recent years. This paper proposes a binary statistical method for highway construction based on project cost control and a construction manag...
CYP102A1 (BM3) is a catalytically self-sufficient flavocytochrome fusion protein isolated from Bacillus megaterium, which displays similar metabolic capabilities to many drug-metabolizing human P450 isoforms. BM3′s high catalytic efficiency, ease of...
Understanding the structure–function relationships of pyridoxal-5′-phosphate (PLP)-dependent transaminases is key to advancing pyridoxal-phosphate-dependent catalysis and engineering transaminases for industrial applications. Despite our...
Metal phase molybdenum disulfide (1T-MoS2) is considered a promising electrocatalyst for the hydrogen evolution reaction (HER). In this work, an interface engineering-induced strategy is reported to prepare a 1T-MoS2/NiS heterostructure. The 1T-MoS2/...
Regardless of its good electron-transfer ability and chemical stability, pure Zn2SnO4 (ZSO) still has intrinsic deficiencies of a narrow spectral response region, poor absorption ability, and high photo-activated carrier recombination rate. Aiming to...
Studies related to the engineering of calcium binding sites of CGTase are limited. The calcium binding regions that are known for thermostability function were subjected to site-directed mutagenesis in this study. The starting gene-protein is a varia...
Sucrose phosphorylase (SPase) is a member of the glycoside hydrolase family 13, catalyzing the reversible phosphorolysis of sucrose to produce α–glucose–1–phosphate and exhibiting transglycosylation activity toward multiple su...
β-Galactosidase from Arthrobacter sp. 32cB (ArthβDG) is a cold-adapted enzyme able to catalyze hydrolysis of β-d-galactosides and transglycosylation reaction, where galactosyl moiety is being transferred onto an acceptor larger than a...
The Rhodococcus erythropolis (strain IGTS8) bacterium has a tremendous industrial interest as it can remove sulfur from crude oil through its four-enzyme (DszA-D) 4S metabolic pathway. DszC is one of the rate-limiting enzymes of the pathway and the o...
Theoretically, a DNA sequence-specific recognition protein that can distinguish a DNA sequence equal to or more than 16 bp could be unique to mammalian genomes. Long-sequence-specific nucleases, such as naturally occurring Homing Endonucleases and ar...
The accumulation of polyethylene terephthalate (PET) seriously harms the environment because of its high resistance to degradation. The recent discovery of the bacteria-secreted biodegradation enzyme, PETase, sheds light on PET recycling; however, th...
Transition-metal sulfides exaggerate higher theoretical capacities and were considered a type of prospective nanomaterials for energy storage; their inherent weaker conductivities and lower electrochemical active sites limited the commercial applicat...
Halohydrin dehalogenases (HHDH) are industrially relevant biocatalysts exhibiting a promiscuous epoxide-ring opening reactivity in the presence of small nucleophiles, thus giving access to novel carbon–carbon, carbon–oxygen, carbon–...
The strategic engineering of metal–organic frameworks (MOFs) through integration with clay minerals offers a promising route to tailor their functional properties and expand their application scope. In this study, a series of clay-MOF composite...
Current clinically used delivery methods for bone morphogenetic proteins (BMPs) are collagen based and require large concentrations that can lead to dangerous side effects. Fibrin hydrogels can serve as osteoinductive bone substitute materials in non...
The oxidation of alcohols to the corresponding carbonyl or carboxyl compounds represents a convenient strategy for the selective introduction of electrophilic carbon centres into carbohydrate-based starting materials. The O2-dependent oxidation of pr...
Cytochrome P450 monooxygenases (CYPs; P450s) are a superfamily of heme-containing enzymes that are recognized for their vast substrate range and oxidative multifunctionality. CYP107 family members perform hydroxylation and epoxidation processes, prod...
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