Search Results (7,210)

Thyroid cancer (TC) incidence is rising, necessitating a refined understanding of its complex biology, particularly for advanced forms. This review synthesizes the state-of-the-art knowledge, guided by the WHO 5th Classification (2022), which incorporates molecular findings and introduces categories like Differentiated High-Grade Thyroid Carcinoma (DHGTC) to better stratify prognosis. The review summarizes the molecular changes in thyroid cancer (TC) by establishing a clear link between specific oncogenic alterations and the resulting tumor phenotype, prognosis, risk stratification and therapeutic vulnerabilities. The central importance of the review lies in its comprehensive integration of these molecular changes with the resulting immunological microenvironment and the rationale for novel, personalized therapies. Moreover, high-level genomic instability within aggressive thyroid malignancies promotes an immunosuppressive tumor microenvironment via the selection and recruitment of suppressive immune components, contributing to immune evasion and poor prognosis. This characteristic immunosuppression identifies the aggressive tumors as prime candidates for targeted immunotherapies. The review implicitly argues that understanding the molecular drivers of this immunosuppression is essential for designing effective clinical trials using these novel agents. Diagnostic advancements, including molecular testing for high-risk mutations (BRAF, TERT) and the integration of Artificial Intelligence (AI) for refined risk stratification, are enabling personalized treatment. The evolving molecular and clinical understanding allows for a paradigm shift toward individualized therapies that balance optimal disease control with minimizing morbidity, especially in the context of high-risk disease. Full article

Background: Currently, there is a considerable number of studies addressing vagus nerve stimulation (VNS) for the treatment of different stroke-related outcomes. We aimed to promote a broad view of the outcomes studied and what are the opportune outcomes to be studied involving this therapeutic strategy for the treatment of post-stroke complications. Methods: This is a scoping review that followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Two investigators conducted independent searches on PubMed/MEDLINE, Scopus, and Embase till July 2025. Randomized clinical trials and preclinical studies using invasive or non-invasive vagus nerve stimulation conducted with a population diagnosed with stroke were included. Results: Forty-one experimental studies and sixteen clinical trials were included. The outcomes found were neuroprotection; motor, functional, and cognitive rehabilitation; dysphagia; comparison of different stimulation intensities; safety, efficacy, and feasibility of the non-invasive approach; comparison between transcutaneous auricular vagus nerve stimulation (taVNS) and transcutaneous cervical vagus nerve stimulation (tcVNS); and comparison between two models of ischemia (permanent and transient). Preclinical studies mostly investigated molecular elements involved in neuroprotection, neuroinflammation, and cellular apoptosis, while clinical studies evaluating the effectiveness of this technique used for rehabilitation and its comparison or combination with other techniques remain scarce. Conclusions: Most studies investigating the effects of VNS on different post-stroke outcomes are experimental studies. Clinical studies are still scarce and with limited analysis of outcomes. Full article

Sward structure and post-grazing heights (SH) significantly influence plant growth and animal intake, crucial for dairy grazing systems. However, these interactions are dynamic and vary with season, resource heterogeneity, and defoliation patterns. Seasonal effects of control (TC), medium (TM), and lax (TL) post-grazing SH of grazed Lolium arundinaceum-based pasture on forage production and utilization, herbage mass, green cover, and chemical composition were tested during autumn-winter and spring seasons and among tall (TP), medium (MP), and short (SP) patches in spring. Thirty-six lactating Holstein cows were randomized evenly to TC, TM, and TL grazing treatments to achieve 6, 9, and 12 cm of post-grazing SH during autumn-winter, and 9, 12, and 15 cm in spring. Forage production was higher on TL than TM and TC, yet utilization was similar across all treatments. The TP relative to MP on SP increased for TL compared to TC and TM. The TP-TC presented higher leaf-density and leaf-proportion, than TP-TL, without modifying leaf canopy distribution of superior-medium horizons among treatments. Grazing management modulated forage production and structural heterogeneity across SH treatments. Critically, monitoring patch-level dynamics—rather than mean height —is essential for optimizing production and harvest efficiency in temperate systems by improving grazing horizon accessibility. Full article

The background error covariance matrix (BE) is a fundamental component of data assimilation (DA) systems. Its impact on both the DA process and subsequent forecast performance depends on model configuration and the types of observations assimilated. However, few studies have specifically examined BE behavior in the context of satellite DA for regional tropical cyclone (TC) prediction. In this study, we develop the BE and evaluate its structure for a TC forecasting system over the western North Pacific. A total of six BEs are modeled using three control variable (CV) schemes (aligned with the CV5, CV6, and CV7 options available in the Weather Research and Forecasting DA system (WRFDA)) with training data from two distinct periods: the TC season and the winter season. Results demonstrate that the BE structure is sensitive to the training data used. The performance of TC-season BEs derived from different CV schemes is assessed for TC track forecasting through the assimilation of microwave sounder satellite brightness temperature data. The evaluation is based on a set of 14 cases from 2018 that exhibited large official track forecast errors. The CV7 BE, which uses the x- and y-direction wind components as CVs, captures finer small-scale momentum error features and yields greater forecast improvement at shorter lead-times (24 h). In contrast, the CV6 BE, which employs stream function (ψ) and unbalanced velocity potential (χ_u) as CVs, incorporates more large-scale momentum error information. The inherent multivariate couplings among analysis variables in this scheme also allow for closer fits to satellite microwave brightness temperature data, which is particularly crucial for forecasting TCs that primarily develop over oceans where conventional observations are scarce. Consequently, it enhances the large-scale environmental field more effectively and delivers superior forecast skill at longer lead times (48 h and 72 h). Full article

Background and Objectives: This study was designed to evaluate the potential cardioprotective effect of Exenatide against doxorubicin (DOX)-induced myocardial injury in rats by assessing scintigraphic alterations together with oxidative stress and inflammation. Materials and Methods: This study included 28 adult male Wistar albino rats that were randomized to 4 groups (n = 7): control, Exenatide alone, DOX (receiving DOX (18 mg/kg, i.p) on days 5–7; Exenatide + DOX (treated with Exenatide together with the DOX). On day 8, ECG, 99mTc-PYP scintigraphy, and biochemical parameters were evaluated. Results: DOX caused ECG abnormalities—bradycardia, significant QT prolongation, and elevated ST-segment amplitude—along with increased myocardial PYP uptake. Exenatide + DOX group significantly improved ECG changes. Biochemically, DOX markedly increased cardiac injury biomarkers (cTnT, CK, CK-MB), hepatic and renal injury markers (ALT, AST, LDH, BUN, creatinine), SIRT-1 level, inflammatory marker (NF-κB, TNF-α, IL-6, NO) and oxidative stress indicators (MDA, TOS), while decreasing antioxidant defenses (GSH, TAS, Nrf2). Exenatide co-treatment significantly attenuated all DOX-induced changes. Conclusions: Exenatide markedly attenuates DOX-induced cardiotoxicity by improving electrical conduction, reducing myocardial radiotracer uptake, and restoring oxidative–inflammatory balance through partial recovery of the SIRT-1/Nrf2/NF-κB pathway. Full article

Microbial communities associated with animal cadaver decomposition play a crucial role in biogeochemical cycles in both aquatic and terrestrial ecosystems. However, it remains unclear regarding the diversity, succession, and assembly of phosphate-solubilizing microbes during animal cadaver decay. In this study, plateau pikas (Ochotona curzoniae) as mammal degradation models were placed on alpine meadow soils to study diversity, succession and assembly of phosphate-solubilizing microbes using amplicon sequencing of phoC- and phoD-genes during 94 days of incubation. The total phosphorus concentration in the corpse group increased by 8.53% on average. Alpha diversity of both phoC- and phoD-harboring microbes decreased in the experimental group compared to the control group, and the community structure differed between control and experimental groups. Phosphate-solubilizing microbial community turnover time rate (TDR) of the experimental group was higher than that of the control group, indicating corpse decay accelerates the succession of phoC- and phoD-harboring microbial community. Null model revealed that deterministic process dominated phoC microbial community in corpse group, while the stochastic process dominated phoD microbial community. The microbial network in experimental group was more complicated than that in control group of phoC microbial community, while phoD microbial community showed opposite trend. Partial least squares path modeling (PLS-PM) showed that phoC-harboring microbial community was mainly influenced by pH, Total carbon (TC) and Total phosphorus (TP), while the phoD microbial community was only regulated by TP. These findings elucidate the ecological mechanism of phosphorus-solubilizing microbial community changes during animal corpse degradation. Full article

This study evaluated the effects of different UV-C radiation doses combined with modified atmosphere packaging (MAP) on the conservation of minimally processed grated anco squash. The squash, obtained from producers in Santiago del Estero (Argentina), was washed, sanitized, cut, peeled, grated, and centrifuged. It was then subjected to UV-C treatments of 5 kJ/m² (T5), 15 kJ/m² (T15), 30 kJ/m² (T30), and 50 kJ/m² (T50). An immersion treatment with NaClO (100 ppm, 3 min) (TH) and an untreated control (TC) were also included. All samples were packaged in PVC trays and sealed with 35 μm polypropylene film, forming a passive MAP. Treatments T5 and T15 preserved acceptable sensory quality for up to 8 days, and no significant differences in color parameters were observed among treatments during storage. Overall, PC decreased by 12–20% and C by 15–37%, while AC increased by 15–40% after 8 days. Treatments T15, T30, and T50 effectively reduced psychrophilic microorganisms for up to 4 days, achieving reductions of 1–2 log compared to TH and TC (6 log CFU/g). By day 8, all treatments reached the microbial limit. In conclusion, the T15 treatment was the most suitable for preserving grated anco squash for up to 4 days at 5 °C, offering a potential alternative to sodium hypochlorite–based sanitization. Full article

At-rest PPG signals have been explored for detecting atrial fibrillation (AF), yet current signal-processing techniques do not achieve perfect accuracy even under low-motion artifact (MA) conditions. This study evaluates the effectiveness of a single-degree-of-freedom time–frequency (SDOF-TF) method in analyzing at-rest PPG signals for AF detection. The method leverages the influence of MA on the instant parameters of each harmonic, which is identified using an SDOF model in which the tissue–contact–sensor (TCS) stack is treated as an SDOF system. In this model, MA induces baseline drift and time-varying system parameters. The SDOF-TF method enables the quantification and removal of MA and noise, allowing for the accurate extraction of the arterial pulse waveform, heart rate (HR), heart rate variability (HRV), respiration rate (RR), and respiration modulation (RM). Using data from the MIMIC PERform AF dataset, the method achieved 100% accuracy in distinguishing AF from non-AF cases based on three features: (1) RM, (2) HRV derived from instant frequency and instant initial phase, and (3) standard deviation of HR across harmonics. Compared with non-AF, the RM for each harmonic was increased by AF. RM exhibited an increasing trend with harmonic order in non-AF subjects, whereas this trend was diminished in AF subjects. Full article

This study investigated the effects of amylose/amylopectin (AM/AP) ratios in low-protein (LP) diets on the growth performance, fat deposition, and nutrient utilization in goslings. A total of 288 healthy, 35-day-old male Jiangnan White Geese were randomly divided into four treatment groups: one group fed a normal protein diet (16%) with an AM/AP ratio of 0.34 (NPR_0.34), and three groups fed low protein diets (14%) with different AM/AP ratios (LPR_0.26, LPR_0.34, LPR_0.44). Each group consisted of six replicates, with 12 geese per replicate, and they were fed for 28 days. The results showed that the body weight at 63 days and average daily gain (ADG) of the LPR_0.44 group geese were significantly higher than those of the other groups (p < 0.01), while the feed-to-gain ratio (F/G) was lower (p < 0.05). The abdominal and mesenteric fat contents were lower in the LPR_0.44 group than in the LPR_0.26 group (p < 0.05), whereas the breast and leg muscle yields were higher (p < 0.05). The breast muscle redness (a*) of the LPR_0.34 and LPR_0.44 groups was higher than in the NPR_0.34 group at 45 min (p < 0.05). The levels of C6:0, C8:0, C11:0, C12:0, and C13:0 in breast muscle saturated fatty acids (SFAs) of the LPR_0.44 group were higher, while that of C18:0 was lower compared with the LPR_0.26 group (p < 0.05). The serum total cholesterol (TC) and triglycerides (TGs) in the LPR_0.44 group were lower than in the LPR_0.26 group (p < 0.05). Hepatic lipase (HL) activity was significantly lower in the LPR_0.44 group (p < 0.01). Regarding hepatic fatty acids, the levels of butyric acid (C4:0), lauric acid (C12:0), and nervonic acid (C24:1) were lower in the LPR_0.44 group than in the LPR_0.26 group (p < 0.05). No significant differences were observed in intestinal morphology, digestive enzyme activities, or nutrient utilization among the groups. (p > 0.05). In conclusion, adjusting the AM/AP ratio to 0.44 in a low-protein diet improved growth performance, regulated lipid metabolism, and maintained intestinal function in goslings. Full article

Six new diterpenoids, including two verticillane ghardaqenoids A–B (1–2) and four dolabellane ghardaqenoids C–F (3–6), were isolated from the soft coral Heteroxenia ghardaqensis collected in the South China Sea. The structures of ghardaqenoids A, D, and E (1, 4, 5) were determined by X-ray diffraction. Ghardaqenoids B, C, and F (2, 3, 6) were identified on the basis of NMR data, DP4+, and ECD spectral data. In particular, compound 6 exhibited strong in vitro lipid-lowering activity in free fatty acid (FFA)-induced HepG2 cells and liver organoids. Further mechanistic studies revealed that compound 6 regulated AMPK-related proteins and genes, thereby inhibiting the accumulation of triglycerides (TG) and total cholesterol (TC). These findings suggested that pharmacological AMPK activation serves as a promising role in lipid-lowering therapeutic strategies. Full article

Chagas disease remains a significant neglected tropical disease that predominantly affects vulnerable populations in rural, low-income areas of Latin America. The management of this condition is severely hindered by the limitations of current therapies, which are characterized by substantial toxicity, diminished efficacy during the chronic phase, and the emergence of parasitic resistance. Given the promising activity of SB-83 (a 2-aminothiophenic derivative) against Leishmania spp., the present study sought to evaluate its trypanocidal activity against Trypanosoma cruzi. The results showed that SB-83 exhibited potent inhibitory effects on the epimastigote forms of T. cruzi (IC₅₀ = 6.23 ± 0.84 μM), trypomastigotes (EC₅₀ = 7.31 ± 0.52 μM) and intracellular amastigotes (EC₅₀ = 5.12 ± 0.49 μM). Furthermore, the cellular proliferation assay results indicated CC₅₀ values of 77.80 ± 2.05 µM for LLC-MK2 CCL-7 and 24.21 ± 1.2 µM for Vero CCL-87, with a selectivity index above 10 for LLC-MK2 cells. In addition, the compound increased TNF-α, IL-12, nitric oxide, and ROS while decreasing IL-10. Moreover, in silico and in vitro assays confirmed its binding to trypanothione reductase, disrupting redox balance. Flow cytometry further revealed apoptosis induction in trypomastigotes, whereas electron microscopy showed cellular disruption and organelle disorganization. Therefore, SB-83 demonstrated potent activity against the TcI-resistant strain linked to Chagas cardiomyopathy at non-toxic concentrations for host cells, supporting its potential as a therapeutic candidate. Full article

N6-methyladenosine (m6A), a predominant and reversible modification of mammalian RNA, plays a critical role in regulating growth, development, and metabolism. While methyltransferase-like 14 (METTL14) is an essential component of the m6A methyltransferase complex, its specific function in regulating milk fat metabolism in dairy goats remains unexplored. This study therefore aimed to elucidate the role of METTL14 in lipid metabolism within dairy goat mammary epithelial cells (GMECs). METTL14 overexpression significantly promoted the synthesis of TAG (Triacylglycerol) and TC (Total cholesterol), as well as lipid droplet accumulation in GMECs. Furthermore, METTL14 upregulated CCAAT enhancer binding protein beta (CEBPB) expression at both the mRNA and protein levels by directly inducing m6A modification on its transcripts. Finally, we confirmed that m6A modification occurs specifically at site 1662 of CEBPB mRNA, and the “Readers” YTH N6-methyladenosine RNA binding protein F1 and F3 (YTHDF1/3) were found responsible for the m6A site recognition and interpretation. This study demonstrated that METTL14 facilitates lipid synthesis and deposition in GMECs. Mechanistically, METTL14 installs the m6A modification at site 1662 of CEBPB transcripts. This m6A mark is specifically recognized by the readers YTHDF1 and YTHDF3, which promote the translation of CEBPB mRNA, thereby upregulating its expression. Full article

The ceramic defect detection technology based on deep learning suffers from the problems of scarce rare defect samples and class imbalance. However, the current deep generative image augmentation techniques are limited when applied to the task of augmenting rare ceramic defects due to issues such as uneven image brightness and insufficient features of small-sized defects, resulting in poor image quality and limited improvement in detection results. This paper proposes a ceramic rare defect image augmentation method based on TC-CycleGAN. TC-CycleGAN is based on the CycleGAN framework and optimizes the generator and discriminator structures to make them more suitable for ceramic defect features, thereby improving the quality of generated images. The generator is TC-UNet, which introduces the scSE and DehazeFormer modules on the basis of UNet, effectively enhancing the model’s ability to learn the subtle defect features on the ceramic surface; the discriminator is the TC-PatchGAN architecture, which replaces the original BatchNorm module with the ContraNorm module, effectively increasing the discriminator’s sensitivity to the representation of tiny ceramic defect features and enhancing the diversity of generated images. The image quality assessment experiments show that the method proposed in this paper significantly improves the quality of generated defective images. For the concave type images, the FID and KID values have decreased by 49% and 73%, respectively, while for the smoke stains type images, the FID and KID values have decreased by 57% and 63% respectively. The further defect detection experiments results show that when using the data set expanded by the method in this paper for training, the recognition accuracy of the detection model for rare defects has significantly improved. The detection accuracy of the concave and smoke stains types of defects has increased by 1.2% and 3.9% respectively. Full article

This study examines the influence of sintering temperature on the structural and transport properties of GdBa₂Cu₃O₇ (Gd123) superconductors prepared from nano-sized precursors via the co-precipitation method. The metal-oxalate precursor (average particle size < 50 nm) was calcined at 900 °C for 12 h, and then the prepared pellets were sintered under an oxygen atmosphere in the range of 920–950 °C for 15 h. All samples showed metallic properties and a sharp superconducting transition. Critical temperatures T_C(R=0) were 94–95 K, with higher sintering temperatures steadily boosting critical current density. X-ray diffraction confirmed orthorhombic Gd123 as the dominant phase, with its phase fraction increasing from 92% to 99.8% as the sintering temperature increased. SEM micrographs showed large, densely packed grains, with higher sintering temperatures promoting improved grain connectivity and reduced porosity. The sample sintered at 950 °C exhibited the most favorable transport performance, attributed to enhanced intergranular coupling and the presence of nanoscale secondary phases acting as effective flux-pinning centers. Overall, these results demonstrate that careful control of sintering temperature can significantly optimize the microstructure and superconducting properties of Gd123 materials, supporting their advancement for practical electrical and magnetic applications. Full article

We compared scatter correction (SC) in single-photon emission computed tomography (SPECT) images using effective scatter source estimation (ESSE) and the triple-energy window (TEW) method. We acquired ^99mTc and ¹²³I images of brain, myocardial, and performance phantoms containing rods with different diameters. We assessed contrast ratios (CRs) and ROI-based noise metrics (coefficient of variation, signal-to-noise ratio, and contrast-to-noise ratio [CNR] ). Under ^99mTc, ESSE yielded higher CRs than TEW across all phantoms (mean difference 0.04, range 0.01–0.05) and produced the highest CNR in the myocardial phantom, improving the conspicuousness of the simulated defect. Under ¹²³I, CR differences between ESSE and TEW were small and inconsistent (performance phantom: −0.04 to 0.06; brain phantom: −0.01 to 0.00). A Monte Carlo simulation (point source in air) showed substantial photopeak window penetration for cardiac high-resolution collimators (40.0%) but low penetration for medium-energy general-purpose collimators (5.1%), supporting photopeak contamination as a contributor to the ¹²³I findings and potentially attenuating the apparent advantage of model-based SC that does not explicitly account for penetration photons. These findings suggest that SC selection should consider the radionuclide and imaging target and that ESSE might be a reasonable option for ^99mTc myocardial imaging under the settings examined. Full article