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Article

[99mTc]Tc-Sestamibi Bioaccumulation Can Induce Apoptosis in Breast Cancer Cells: Molecular and Clinical Perspectives

1
Nuclear Medicine Unit, Department of Oncohaematology, Policlinico “Tor Vergata”, 00133 Rome, Italy
2
Department of Experimental Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, Italy
3
San Raffaele University, Via di Val Cannuta 247, 00166 Rome, Italy
4
Saint Camillus International University of Health Sciences, Via di Sant’Alessandro, 8, 00131 Rome, Italy
5
Tor Vergata Oncoscience Research (TOR), University of Rome “Tor Vergata”, 00133 Rome, Italy
6
Diagnostica Medica & ‘Villa dei Platani’, Neuromed Group, 83100 Avellino, Italy
7
IRCCS Neuromed, Via Atinense, 18, 8607 Pozzilli, Italy
8
Department of Biomedicine and Prevention, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, Italy
*
Author to whom correspondence should be addressed.
Nicoletta Urbano and Manuel Scimeca equally first author.
Academic Editor: Salvatore Gallo
Appl. Sci. 2021, 11(6), 2733; https://doi.org/10.3390/app11062733
Received: 3 March 2021 / Revised: 15 March 2021 / Accepted: 16 March 2021 / Published: 18 March 2021
(This article belongs to the Special Issue Applications of Medical Physics)
The aim of this study was to investigate the possible role of [99mTc]Tc-Sestamibi in the regulation of cancer cell proliferation and apoptosis. To this end, the in vivo values of [99mTc]Tc-Sestamibi uptake have been associated with the in-situ expression of both Ki67 and caspase-3. For in vitro investigations, BT-474 cells were incubated with three different concentrations of [99mTc]Tc-Sestamibi: 10 µg/mL, 1 µg/mL, and 0.1 µg/mL. Expression of caspase-3 and Ki67, as well as the ultrastructure of cancer cells, was evaluated at T0 and after 24, 48, 72, and 120 h after [99mTc]Tc-Sestamibi incubation. Ex vivo data strengthened the known association between sestamibi uptake and Ki67 expression. Linear regression analysis showed a significant association between sestamibi uptake and the number of apoptotic cells evaluated as caspase-3-positive breast cancer cells. As concerning the in vitro data, a significant decrease of the proliferation index was observed in breast cancer cells incubated with a high concentration of [99mTc]Tc-Sestamibi (10 µg/mL). Amazingly, a significant increase in caspase-3-positive cells in cultures incubated with 10 µg/mL [99mTc]Tc-Sestamibi was observed. This study suggested the possible role of sestamibi in the regulation of pathophysiological processes involved in breast cancer. View Full-Text
Keywords: 99mTc-sestamibi; breast-specific γ imaging; breast cancer; apoptosis; theragnostic 99mTc-sestamibi; breast-specific γ imaging; breast cancer; apoptosis; theragnostic
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MDPI and ACS Style

Urbano, N.; Scimeca, M.; Bonfiglio, R.; Mauriello, A.; Bonanno, E.; Schillaci, O. [99mTc]Tc-Sestamibi Bioaccumulation Can Induce Apoptosis in Breast Cancer Cells: Molecular and Clinical Perspectives. Appl. Sci. 2021, 11, 2733. https://doi.org/10.3390/app11062733

AMA Style

Urbano N, Scimeca M, Bonfiglio R, Mauriello A, Bonanno E, Schillaci O. [99mTc]Tc-Sestamibi Bioaccumulation Can Induce Apoptosis in Breast Cancer Cells: Molecular and Clinical Perspectives. Applied Sciences. 2021; 11(6):2733. https://doi.org/10.3390/app11062733

Chicago/Turabian Style

Urbano, Nicoletta, Manuel Scimeca, Rita Bonfiglio, Alessandro Mauriello, Elena Bonanno, and Orazio Schillaci. 2021. "[99mTc]Tc-Sestamibi Bioaccumulation Can Induce Apoptosis in Breast Cancer Cells: Molecular and Clinical Perspectives" Applied Sciences 11, no. 6: 2733. https://doi.org/10.3390/app11062733

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