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191 Results Found

  • Article
  • Open Access
7 Citations
5,377 Views
15 Pages

Comparative Analysis and Ancestral Sequence Reconstruction of Bacterial Sortase Family Proteins Generates Functional Ancestral Mutants with Different Sequence Specificities

  • Jordan D. Valgardson,
  • Sarah A. Struyvenberg,
  • Zachary R. Sailer,
  • Isabel M. Piper,
  • Justin E. Svendsen,
  • D. Alex Johnson,
  • Brandon A. Vogel,
  • John M. Antos,
  • Michael J. Harms and
  • Jeanine F. Amacher

9 June 2022

Gram-positive bacteria are some of the earliest known life forms, diverging from gram-negative bacteria 2 billion years ago. These organisms utilize sortase enzymes to attach proteins to their peptidoglycan cell wall, a structural feature that distin...

  • Article
  • Open Access
11 Citations
4,629 Views
20 Pages

Taxonomy, Phylogenetic and Ancestral Area Reconstruction in Phyllachora, with Four Novel Species from Northwestern China

  • Jin-Chen Li,
  • Hai-Xia Wu,
  • Yuying Li,
  • Xin-Hao Li,
  • Jia-Yu Song,
  • Nakarin Suwannarach and
  • Nalin N. Wijayawardene

18 May 2022

The members of Phyllachora are biotrophic, obligate plant parasitic fungi featuring a high degree of host specificity. This genus also features a high degree of species richness and worldwide distribution. In this study, four species occurring on lea...

  • Article
  • Open Access
138 Views
16 Pages

15 January 2026

The bifunctional glutathione synthase (GshF) is able to catalyze glutathione synthesis and is favored for industrial application due to its lack of product inhibition. However, its practical use is limited by moderate catalytic efficiency and poor th...

  • Article
  • Open Access
2 Citations
3,094 Views
9 Pages

5 October 2022

As a biologically active peptide, L-carnosine has been widely used in the pharmaceutical, cosmetic and health care industries due to its various physiological properties. However, relatively little research is available regarding L-carnosine’s...

  • Review
  • Open Access
28 Citations
9,742 Views
14 Pages

6 August 2017

Understanding the evolution of ancestral life, and especially the ability of some organisms to flourish in the variable environments experienced in Earth’s early biosphere, requires knowledge of the characteristics and the environment of these ancest...

  • Article
  • Open Access
6 Citations
10,460 Views
21 Pages

REGEN: Ancestral Genome Reconstruction for Bacteria

  • Kuan Yang,
  • Lenwood S. Heath and
  • João C. Setubal

18 July 2012

Ancestral genome reconstruction can be understood as a phylogenetic study with more details than a traditional phylogenetic tree reconstruction. We present a new computational system called REGEN for ancestral bacterial genome reconstruction at both...

  • Article
  • Open Access
6 Citations
3,464 Views
13 Pages

Validation of Automated Chromosome Recovery in the Reconstruction of Ancestral Gene Order

  • Qiaoji Xu,
  • Lingling Jin,
  • James H. Leebens-Mack and
  • David Sankoff

21 May 2021

The RACCROCHE pipeline reconstructs ancestral gene orders and chromosomal contents of the ancestral genomes at all internal vertices of a phylogenetic tree. The strategy is to accumulate a very large number of generalized adjacencies, phylogeneticall...

  • Article
  • Open Access
3 Citations
2,642 Views
15 Pages

22 April 2024

Xylanase is an essential component used to hydrolyze the xylan in wheat flour to enhance the quality of bread. Presently, cold-activated xylanase is popularly utilized to aid in the development of dough. In this study, ancestral sequence reconstructi...

  • Article
  • Open Access
2 Citations
3,581 Views
14 Pages

Structural Analysis of the Ancestral Haloalkane Dehalogenase AncLinB-DmbA

  • Andrii Mazur,
  • Pavel Grinkevich,
  • Radka Chaloupkova,
  • Petra Havlickova,
  • Barbora Kascakova,
  • Michal Kuty,
  • Jiri Damborsky,
  • Ivana Kuta Smatanova and
  • Tatyana Prudnikova

5 November 2021

Haloalkane dehalogenases (EC 3.8.1.5) play an important role in hydrolytic degradation of halogenated compounds, resulting in a halide ion, a proton, and an alcohol. They are used in biocatalysis, bioremediation, and biosensing of environmental pollu...

  • Article
  • Open Access
1,283 Views
43 Pages

Coevolution of Lentiviral Vif with Host A3F and A3G: Insights from Computational Modelling and Ancestral Sequence Reconstruction

  • David Nicolas Giuseppe Huebert,
  • Atefeh Ghorbani,
  • Shaw Yick Brian Lam and
  • Mani Larijani

10 March 2025

The evolutionary arms race between host restriction factors and viral antagonists provides crucial insights into immune system evolution and viral adaptation. This study investigates the structural and evolutionary dynamics of the double-domain restr...

  • Article
  • Open Access
17 Citations
4,620 Views
12 Pages

The Early Terrestrial Fungal Lineage of Conidiobolus—Transition from Saprotroph to Parasitic Lifestyle

  • Andrii P. Gryganskyi,
  • Yong Nie,
  • Ann E. Hajek,
  • Kathie T. Hodge,
  • Xiao-Yong Liu,
  • Kelsey Aadland,
  • Kerstin Voigt,
  • Iryna M. Anishchenko,
  • Vira B. Kutovenko and
  • Jason E. Stajich
  • + 4 authors

28 July 2022

Fungi of the Conidiobolus group belong to the family Ancylistaceae (Entomophthorales, Entomophthoromycotina, Zoopagomycota) and include over 70 predominantly saprotrophic species in four similar and closely related genera, that were separated phyloge...

  • Article
  • Open Access
10 Citations
4,856 Views
24 Pages

Keeping 21st Century Paleontology Grounded: Quantitative Genetic Analyses and Ancestral State Reconstruction Re-Emphasize the Essentiality of Fossils

  • Tesla A. Monson,
  • Marianne F. Brasil,
  • Michael C. Mahaney,
  • Christopher A. Schmitt,
  • Catherine E. Taylor and
  • Leslea J. Hlusko

13 August 2022

Advances in genetics and developmental biology are revealing the relationship between genotype and dental phenotype (G:P), providing new approaches for how paleontologists assess dental variation in the fossil record. Our aim was to understand how th...

  • Article
  • Open Access
10 Citations
4,299 Views
8 Pages

25 July 2019

The genus Aedes is well known for its desiccation-resistant eggs, which frequently serve as an overwintering mechanism through diapause. Despite this, relatively little is known about the diapause and overwintering biology of most Aedes species inclu...

  • Article
  • Open Access
620 Views
17 Pages

Mitogenomic Insights into Phylogeny, Biogeography and Adaptive Evolution of the Genus Typhlomys (Rodentia: Platacanthomyidae)

  • Chao Na,
  • Xiaohan Wang,
  • Yaxin Cheng,
  • Yixin Huang,
  • Shuiwang He,
  • Laxman Khanal,
  • Shunde Chen,
  • Xuelong Jiang and
  • Zhongzheng Chen

27 September 2025

Soft-furred tree mice (genus Typhlomys), which are native to southern China and northern Vietnam, are unique rodents capable of echolocation. Little is known about their taxonomy, ecology, and natural history. In this study, we generated the complete...

  • Article
  • Open Access
2,122 Views
16 Pages

13 March 2025

Ciliates of the genus Frontonia have been extensively studied to resolve their phylogenetic and evolutionary history, but challenges remain. This study used molecular analyses of SSU rRNA genes, phylogenetic tree reconstruction, molecular dating, and...

  • Communication
  • Open Access
1 Citations
2,163 Views
12 Pages

9 April 2024

Aytoniaceae are one of the largest families of complex thalloid liverworts (Marchantiopsida), consisting of about 70 species, with most species being distributed in temperate areas. However, the phylogeny and evolution of the morphological character...

  • Article
  • Open Access
4 Citations
2,309 Views
21 Pages

25 May 2023

A deeper understanding of HIV-1 transmission and drug resistance mechanisms can lead to improvements in current treatment policies. However, the rates at which HIV-1 drug resistance mutations (DRMs) are acquired and which transmitted DRMs persist are...

  • Article
  • Open Access
25 Citations
4,526 Views
18 Pages

Molecular Evolution of Far-Red Light-Acclimated Photosystem II

  • Christopher J. Gisriel,
  • Tanai Cardona,
  • Donald A. Bryant and
  • Gary W. Brudvig

Cyanobacteria are major contributors to global carbon fixation and primarily use visible light (400−700 nm) to drive oxygenic photosynthesis. When shifted into environments where visible light is attenuated, a small, but highly diverse and wide...

  • Article
  • Open Access
14 Citations
4,680 Views
20 Pages

13 November 2020

We investigated the biogeography of Stigmaphyllon, the second-largest lianescent genus of Malpighiaceae, as a model genus to reconstruct the age and biogeographic history of the Brazilian Atlantic Rainforest (BAF). Few studies to date have focused on...

  • Article
  • Open Access
3 Citations
3,464 Views
29 Pages

Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales)

  • Huili Pu,
  • Jiao Yang,
  • Nemat O. Keyhani,
  • Lixia Yang,
  • Minhai Zheng,
  • Chenghao Qiu,
  • Yuchen Mao,
  • Junya Shang,
  • Yongsheng Lin and
  • Junzhi Qiu
  • + 9 authors

2 January 2025

The Cordycipitaceae family of insecticidal fungi is widely distributed in nature, is the most complex in the order Hypocreales (Ascomycota), with members displaying a diversity of morphological characteristics and insect host ranges. Based on Bayesia...

  • Article
  • Open Access
176 Views
36 Pages

In Silico Proof of Concept: Conditional Deep Learning-Based Prediction of Short Mitochondrial DNA Fragments in Archosaurs

  • Dimitris Angelakis,
  • Dionisis Cavouras,
  • Dimitris Th. Glotsos,
  • Spiros A. Kostopoulos,
  • Emmanouil I. Athanasiadis,
  • Ioannis K. Kalatzis and
  • Pantelis A. Asvestas

14 January 2026

This study presents an in silico proof of concept exploring whether deep learning models can perform conditional mitochondrial DNA (mtDNA) sequence prediction across species boundaries. A CNN–BiLSTM model was trained under a leave-one-species-o...

  • Article
  • Open Access
4 Citations
6,325 Views
18 Pages

Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases

  • Marcel Zámocký,
  • Miloš Musil,
  • Maksym Danchenko,
  • Peter Ferianc,
  • Katarína Chovanová,
  • Peter Baráth,
  • Andrej Poljovka and
  • David Bednář

17 March 2022

In this study, we focus on a detailed bioinformatics analysis of hyBpox genes, mainly within the genomes of Sclerotiniaceae (Ascomycota, Leotiomycetes), which is a specifically evolved fungal family of necrotrophic host generalists and saprophytic or...

  • Article
  • Open Access
6 Citations
3,247 Views
12 Pages

29 September 2022

In this study, the mitochondrial genomes of 22 species from three subfamilies in the Sphingidae were sequenced, assembled, and annotated. Eight diurnal hawkmoths were included, of which six were newly sequenced (Hemaris radians, Macroglossum bombylan...

  • Article
  • Open Access
17 Citations
6,566 Views
24 Pages

Phylogeny and Biogeography of Morus (Moraceae)

  • Chen-Xuan Yang,
  • Shui-Yin Liu,
  • Nyree J. C. Zerega,
  • Gregory W. Stull,
  • Elliot M. Gardner,
  • Qin Tian,
  • Wei Gu,
  • Qing Lu,
  • Ryan A. Folk and
  • Ting-Shuang Yi
  • + 5 authors

30 July 2023

The mulberry genus, Morus L. (Moraceae), has long been taxonomically difficult, and its species circumscription has only been defined recently. This genus comprises ca. 16 species distributed across Asia and the Americas, yet its biogeographic histor...

  • Article
  • Open Access
775 Views
16 Pages

Molecular Phylogenetics and Morphological Analyses Support Dolichopoda, a New Neotropical Genus of Marantaceae (Zingiberales)

  • Naédja K. M. Luna,
  • Thales S. Coutinho,
  • Mark W. Chase,
  • Leonardo P. Felix and
  • Edlley M. Pessoa

15 November 2025

This study presents a molecular phylogenetic analysis based on four DNA regions (plastid matK, trnL-trnF, and rps16 and nuclear ribosomal ITS) for Ctenanthe, Saranthe and Stromanthe, encompassing nearly 70% of species in these genera. Given this exte...

  • Article
  • Open Access
3 Citations
2,855 Views
14 Pages

Simplified Genomic Data Revealing the Decline of Aleuritopteris grevilleoides Population Accompanied by the Uplift of Dry-Hot Valley in Yunnan, China

  • Xue-Ying Wei,
  • Ting Wang,
  • Jin Zhou,
  • Wei-Yue Sun,
  • Dong-Mei Jin,
  • Jian-Ying Xiang,
  • Jian-Wen Shao and
  • Yue-Hong Yan

6 April 2023

Understanding the evolutionary history of endangered species is crucial for identifying the main reasons for species endangerment in the past and predicting the changing trends and evolutionary directions of their future distribution. In order to stu...

  • Review
  • Open Access
25 Citations
5,263 Views
37 Pages

Re-Evaluating Botryosphaeriales: Ancestral State Reconstructions of Selected Characters and Evolution of Nutritional Modes

  • Achala R. Rathnayaka,
  • K. W. Thilini Chethana,
  • Alan J. L. Phillips,
  • Jian-Kui Liu,
  • Milan C. Samarakoon,
  • E. B. Gareth Jones,
  • Samantha C. Karunarathna and
  • Chang-Lin Zhao

29 January 2023

Botryosphaeriales (Dothideomycetes, Ascomycota) occur in a wide range of habitats as endophytes, saprobes, and pathogens. The order Botryosphaeriales has not been subjected to evaluation since 2019 by Phillips and co-authors using phylogenetic and ev...

  • Review
  • Open Access
27 Citations
6,948 Views
14 Pages

African Swine Fever Virus Circulation between Tanzania and Neighboring Countries: A Systematic Review and Meta-Analysis

  • Jean N. Hakizimana,
  • Clara Yona,
  • Olivier Kamana,
  • Hans Nauwynck and
  • Gerald Misinzo

15 February 2021

For over 100 years after the description of the first case of African swine fever (ASF) in Kenya, ASF virus (ASFV) cross-border spread in eastern and southern Africa has not been fully investigated. In this manuscript, we reviewed systematically the...

  • Review
  • Open Access
2 Citations
2,619 Views
19 Pages

30 January 2024

How Nature discovered genetic coding is a largely ignored question, yet the answer is key to explaining the transition from biochemical building blocks to life. Other, related puzzles also fall inside the aegis enclosing the codes themselves. The pep...

  • Article
  • Open Access
50 Citations
6,925 Views
14 Pages

16 January 2020

Virus host range, i.e., the number and diversity of host species of viruses, is an important determinant of disease emergence and of the efficiency of disease control strategies. However, for plant viruses, little is known about the genetic or ecolog...

  • Communication
  • Open Access
9 Citations
4,658 Views
11 Pages

Revisiting the Evolution of Arboreal Life in Oribatid Mites

  • Sylvia Schäffer,
  • Stephan Koblmüller and
  • Günther Krisper

22 June 2020

Though mostly soil dwelling, oribatid mites are found in all kind of habitats, with several species exclusively living on trees. Using previously published DNA sequences and eco-morphological data available from the literature, we inferred the number...

  • Article
  • Open Access
15 Citations
7,333 Views
12 Pages

12 November 2020

Wolbachia bacteria are widely distributed across invertebrate taxa, including ants, but several aspects of this host-associated interaction are still poorly explored, especially with regard to the ancestral state association, origin, and dispersion p...

  • Article
  • Open Access
1,596 Views
18 Pages

30 November 2024

Continuous surveillance is critical for early intervention against emerging novel SARS-CoV-2 variants. Therefore, we investigated and compared the variant-specific evolutionary epidemiology of all the Delta and Omicron sequences collected between 202...

  • Article
  • Open Access
12 Citations
4,859 Views
18 Pages

Phylogenetic Relationships, Speciation, and Origin of Armillaria in the Northern Hemisphere: A Lesson Based on rRNA and Elongation Factor 1-Alpha

  • Junmin Liang,
  • Lorenzo Pecoraro,
  • Lei Cai,
  • Zhilin Yuan,
  • Peng Zhao,
  • Clement K. M. Tsui and
  • Zhifeng Zhang

17 December 2021

Armillaria species have a global distribution and play various roles in the natural ecosystems, e.g., pathogens, decomposers, and mycorrhizal associates. However, their taxonomic boundaries, speciation processes, and origin are poorly understood. Her...

  • Article
  • Open Access
177 Views
19 Pages

8 January 2026

This article examines how the Park Chung Hee regime reshaped the public memory of the Neo-Confucian philosopher Yi I (penname Yulgok, 1536–1584) by recasting him as a model of patriotic nationalism. Beginning with the inauguration of the Yulgok...

  • Article
  • Open Access
15 Citations
6,967 Views
7 Pages

Peptidyl Transferase Center and the Emergence of the Translation System

  • Savio Torres de Farias,
  • Thais Gaudêncio Rêgo and
  • Marco V. José

25 April 2017

In this work, the three-dimensional (3D) structure of the ancestral Peptidyl Transferase Center (PTC) built by concatamers of ancestral sequences of tRNAs was reconstructed, and its possible interactions with tRNAs molecules were analyzed. The 3D str...

  • Article
  • Open Access
2 Citations
4,188 Views
25 Pages

Still Many to Be Named: An Integrative Analysis of the Genus Dendronotus (Gastropoda: Nudibranchia) in the North Pacific Revealed Seven New Species

  • Irina A. Ekimova,
  • Anna L. Mikhlina,
  • Maria V. Stanovova,
  • Nellya R. Krupitskaya,
  • Olga V. Chichvarkhina and
  • Dimitry M. Schepetov

23 January 2023

In this paper, we report on the discovery of the hidden biodiversity of the genus Dendronotus (Gastropoda: Nudibranchia) in bathyal areas of the North Pacific (the Sea of Okhotsk and the Kuril Islands). We also test different scenarios of Dendronotus...

  • Article
  • Open Access
2,008 Views
13 Pages

Genetic Insights into the Historical Attribution of Variety Names of Sweet Chestnut (Castanea sativa Mill.) in Northern Italy

  • Marta Cavallini,
  • Gianluca Lombardo,
  • Claudio Cantini,
  • Mauro Gerosa and
  • Giorgio Binelli

1 July 2024

The sweet chestnut (Castanea sativa Mill.) is subject to the progressive disappearance of its traditional chestnut groves. In the northern part of Italy, where distribution of the sweet chestnut is fragmented, many local varieties continue to be iden...

  • Article
  • Open Access
1 Citations
2,372 Views
18 Pages

Distinguishing Genetic Drift from Selection in Papillomavirus Evolution

  • Robert D. Burk,
  • Lisa Mirabello and
  • Robert DeSalle

26 July 2023

Pervasive purifying selection on non-synonymous substitutions is a hallmark of papillomavirus genome history, but the role of selection on and the drift of non-coding DNA motifs on HPV diversification is poorly understood. In this study, more than a...

  • Article
  • Open Access
2 Citations
3,587 Views
17 Pages

Phylogenetic Analysis and Flower Color Evolution of the Subfamily Linoideae (Linaceae)

  • Alejandra Villalvazo-Hernández,
  • Mireya Burgos-Hernández and
  • Dolores González

15 June 2022

The taxonomy of the subfamily Linoideae at the intergeneric and section levels has been questioned throughout the years, and the evolution of floral characters remains poorly understood. In particular, the evolution of flower color is still uncertain...

  • Article
  • Open Access
12 Citations
3,882 Views
17 Pages

The Chromosome Number and rDNA Loci Evolution in Onobrychis (Fabaceae)

  • Gulru Yucel,
  • Alexander Betekhtin,
  • Evren Cabi,
  • Metin Tuna,
  • Robert Hasterok and
  • Bozena Kolano

20 September 2022

The evolution of chromosome number and ribosomal DNA (rDNA) loci number and localisation were studied in Onobrychis Mill. Diploid and tetraploid species, as well as two basic chromosome numbers, x = 7 and x = 8, were observed among analysed taxa. The...

  • Article
  • Open Access
1 Citations
5,324 Views
20 Pages

24 January 2022

Despite many decades of studies, our knowledge of skeletal development in birds is limited in many aspects. One of them is the development of the vertebral column. For many years it was widely believed that the column ossifies anteroposteriorly. Howe...

  • Article
  • Open Access
2 Citations
3,197 Views
17 Pages

17 April 2024

Spirorbinae, a ubiquitous group of marine calcareous tubeworms with a small body size as adults, have a fascinating diversity of brooding modes that form the basis for their taxonomic division into six tribes (traditionally subfamilies): in-tube incu...

  • Article
  • Open Access
6 Citations
7,405 Views
20 Pages

Structural Features of Carnivorous Plant (Genlisea, Utricularia) Tubers as Abiotic Stress Resistance Organs

  • Bartosz J. Płachno,
  • Saura R. Silva,
  • Piotr Świątek,
  • Kingsley W. Dixon,
  • Krzystof Lustofin,
  • Guilherme C. Seber and
  • Vitor F. O. Miranda

Carnivorous plants from the Lentibulariaceae form a variety of standard and novel vegetative organs and survive unfavorable environmental conditions. Within Genlisea, only G. tuberosa, from the Brazilian Cerrado, formed tubers, while Utricularia menz...

  • Article
  • Open Access
3 Citations
5,927 Views
30 Pages

14 August 2017

To contemplate African American experience and its many racialized contours is to invoke a tensive quandary concerning the reconstruction of African American cultural identity in a dynamic network of historically assailed African diasporas. Utilizing...

  • Article
  • Open Access
16 Citations
5,894 Views
16 Pages

Evolution and Biogeographic History of Rubyspot Damselflies (Hetaerininae: Calopterygidae: Odonata)

  • Samantha Standring,
  • Melissa Sánchez-Herrera,
  • Rhainer Guillermo-Ferreira,
  • Jessica L. Ware,
  • Yesenia Margarita Vega-Sánchez,
  • Rebecca Clement,
  • Jonathan P. Drury,
  • Gregory F. Grether,
  • Antonio González-Rodríguez and
  • Seth Bybee
  • + 2 authors

14 September 2022

The damselflies Hetaerininae, a subfamily of Calopterygidae, comprise four genera distributed from North to South America: Hetaerina, Mnesarete, Ormenophlebia and Bryoplathanon. While several studies have focused on the intriguing behavioral and morp...

  • Article
  • Open Access
6 Citations
4,024 Views
12 Pages

26 February 2019

Studying parallel and convergent amino acid replacements in protein evolution is frequently used to assess adaptive evolution at the molecular level. Identifying parallel and convergent replacements involves multiple steps and computational routines,...

  • Article
  • Open Access
6 Citations
4,028 Views
14 Pages

A Natural Colonisation of Asia: Phylogenomic and Biogeographic History of Coin Spiders (Araneae: Nephilidae: Herennia)

  • Eva Turk,
  • Jason E. Bond,
  • Ren-Chung Cheng,
  • Klemen Čandek,
  • Chris A. Hamilton,
  • Matjaž Gregorič,
  • Simona Kralj-Fišer and
  • Matjaž Kuntner

22 October 2021

Reconstructing biogeographic history is challenging when dispersal biology of studied species is poorly understood, and they have undergone a complex geological past. Here, we reconstruct the origin and subsequent dispersal of coin spiders (Nephilida...

  • Article
  • Open Access
6 Citations
8,001 Views
21 Pages

Evolutionary Patterns of Intersexual Power

  • Rebecca J. Lewis,
  • E. Christopher Kirk and
  • Ashley D. Gosselin-Ildari

29 November 2023

Dominance and leverage are both possible causes of social inequality. If sexual dimorphism influences patterns of intersexual dominance, we predicted that highly dimorphic species are constrained to exhibit male-biased power (MP), but species with lo...

  • Article
  • Open Access
12 Citations
4,212 Views
21 Pages

Descending Dysploidy and Bidirectional Changes in Genome Size Accompanied Crepis (Asteraceae) Evolution

  • Magdalena Senderowicz,
  • Teresa Nowak,
  • Magdalena Rojek-Jelonek,
  • Maciej Bisaga,
  • Laszlo Papp,
  • Hanna Weiss-Schneeweiss and
  • Bozena Kolano

17 September 2021

The evolution of the karyotype and genome size was examined in species of Crepis sensu lato. The phylogenetic relationships, inferred from the plastid and nrITS DNA sequences, were used as a framework to infer the patterns of karyotype evolution. Fiv...

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