Comparative Analysis and Ancestral Sequence Reconstruction of Bacterial Sortase Family Proteins Generates Functional Ancestral Mutants with Different Sequence Specificities
Abstract
:1. Introduction
2. Results
2.1. Principal Component Analysis (PCA) of Bacterial Sortases
2.2. Ancestral Sequence Reconstruction of Class A Sortases
2.3. Structural Analyses of Ancestral SrtA Proteins
2.4. Investigating Ancestral Proteins at Distant Nodes
3. Discussion
4. Materials and Methods
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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(a) | |||||||
---|---|---|---|---|---|---|---|
ancStaph | S. agnetis | S. aureus | S. auricularis | S. capitis | S. epidermidis | S. pettenkoferi | |
ancStaph | X | ||||||
S. agnetis | 68% (97/142) | X | |||||
S. aureus | 86% (127/147) | 76% (108/142) | X | ||||
S. auricularis | 73% (107/146) | 62% (91/146) | 70% (101/144) | X | |||
S. capitis | 88% (129/146) | 62% (88/141) | 84% (122/146) | 68% (96/141) | X | ||
S. epidermidis | 81% (116/143) | 59% (84/142) | 78% (114/146) | 69% (98/142) | 84% (124/147) | X | |
S. pettenkoferi | 74% (105/141) | 58% (81/140) | 67% (96/143) | 78% (109/140) | 73% (104/143) | 71% (101/143) | X |
(b) | |||||||
ancStrep | S. agalactiae | S. mutans | S. oralis | S. pneumoniae | S. pyogenes | S. suis | |
ancStrep | X | ||||||
S. agalactiae | 68% (113/165) | X | |||||
S. mutans | 65% (106/163) | 64% (109/169) | X | ||||
S. oralis | 69% (113/163) | 58% (97/166) | 65% (107/165) | X | |||
S. pneumoniae | 68% (111/164) | 57% (95/167) | 64% (107/166) | 81% (136/167) | X | ||
S. pyogenes | 71% (117/164) | 65% (109/168) | 70% (117/168) | 63% (104/166) | 63% (105/166) | X | |
S. suis | 76% (125/164) | 58% (98/168) | 60% (101/168) | 61% (101/165) | 62% (103/166) | 63% (104/166) | X |
ancStaph | ancStrep | ancNode-408 | ancNode-503 | ancNode-547 | |
---|---|---|---|---|---|
ancStaph | X | ||||
ancStrep | 30% (35/117) | X | |||
ancNode-408 | 35% (47/133) | 51% (77/151) | X | ||
ancNode-503 | 33% (50/150) | 56% (76/136) | 78% (156/200) | X | |
ancNode-547 | 54% (64/118) | 59% (85/145) | 64% (147/199) | 77% (168/199) | X |
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Valgardson, J.D.; Struyvenberg, S.A.; Sailer, Z.R.; Piper, I.M.; Svendsen, J.E.; Johnson, D.A.; Vogel, B.A.; Antos, J.M.; Harms, M.J.; Amacher, J.F. Comparative Analysis and Ancestral Sequence Reconstruction of Bacterial Sortase Family Proteins Generates Functional Ancestral Mutants with Different Sequence Specificities. Bacteria 2022, 1, 121-135. https://doi.org/10.3390/bacteria1020011
Valgardson JD, Struyvenberg SA, Sailer ZR, Piper IM, Svendsen JE, Johnson DA, Vogel BA, Antos JM, Harms MJ, Amacher JF. Comparative Analysis and Ancestral Sequence Reconstruction of Bacterial Sortase Family Proteins Generates Functional Ancestral Mutants with Different Sequence Specificities. Bacteria. 2022; 1(2):121-135. https://doi.org/10.3390/bacteria1020011
Chicago/Turabian StyleValgardson, Jordan D., Sarah A. Struyvenberg, Zachary R. Sailer, Isabel M. Piper, Justin E. Svendsen, D. Alex Johnson, Brandon A. Vogel, John M. Antos, Michael J. Harms, and Jeanine F. Amacher. 2022. "Comparative Analysis and Ancestral Sequence Reconstruction of Bacterial Sortase Family Proteins Generates Functional Ancestral Mutants with Different Sequence Specificities" Bacteria 1, no. 2: 121-135. https://doi.org/10.3390/bacteria1020011