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2,619 Results Found

  • Review
  • Open Access
79 Citations
8,554 Views
28 Pages

The Universal Soldier: Enzymatic and Non-Enzymatic Antioxidant Functions of Serum Albumin

  • Daria A. Belinskaia,
  • Polina A. Voronina,
  • Vladimir I. Shmurak,
  • Mikhail A. Vovk,
  • Anastasia A. Batalova,
  • Richard O. Jenkins and
  • Nikolay V. Goncharov

9 October 2020

As a carrier of many biologically active compounds, blood is exposed to oxidants to a greater extent than the intracellular environment. Serum albumin plays a key role in antioxidant defence under both normal and oxidative stress conditions. This rev...

  • Article
  • Open Access
12 Citations
3,388 Views
27 Pages

Enzymatic Antioxidant Defense and Polymorphic Changes in Male Infertility

  • Jędrzej Baszyński,
  • Piotr Kamiński,
  • Maria Bogdzińska,
  • Sławomir Mroczkowski,
  • Marek Szymański,
  • Karolina Wasilow,
  • Emilia Stanek,
  • Karolina Hołderna-Bona,
  • Sylwia Brodzka and
  • Alina Woźniak
  • + 5 authors

The intensification of oxidative stress and destabilization of the antioxidative defenses of an organism is a consequence of many environmental factors. We considered aspects conditioning male reproductive potential and the functionality of enzymatic...

  • Article
  • Open Access
4 Citations
2,595 Views
12 Pages

29 November 2023

Obtaining peptides with antioxidant properties by enzymatic hydrolysis has been widely described; however, the use of non-enzymatic methods to obtain peptides with antioxidant capacities has been poorly investigated. In this study, non-soluble protei...

  • Article
  • Open Access
637 Views
19 Pages

21 November 2025

Jellyfish, as high-biomass marine resources, frequently exhibit explosive proliferation in coastal waters and possess both nutritional functional factors and potential medicinal value. This study investigated the enzymatic hydrolysis of two jellyfish...

  • Article
  • Open Access
5 Citations
2,070 Views
20 Pages

Lactiplantibacillus plantarum for the Preparation of Fermented Low-Bitter Enzymatic Skim Milk with Antioxidant Ability

  • Yi Jiang,
  • Longfei Zhang,
  • Yushi Jin,
  • Haiyan Xu,
  • Yating Liang,
  • Zihan Xia,
  • Chenchen Zhang,
  • Chengran Guan,
  • Hengxian Qu and
  • Dawei Chen
  • + 4 authors

27 November 2024

A high degree of hydrolysis can reduce the allergenicity of milk, while lactic acid bacteria (LAB) fermentation can further enhance the antioxidant ability of enzymatic milk. LAB with a strong antioxidant ability was screened, and the effects of LAB...

  • Article
  • Open Access
24 Citations
9,560 Views
18 Pages

Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies

  • Arijit Nath,
  • Geremew Geidare Kailo,
  • Zsuzsanna Mednyánszky,
  • Gabriella Kiskó,
  • Barbara Csehi,
  • Klára Pásztorné-Huszár,
  • Renáta Gerencsér-Berta,
  • Ildikó Galambos,
  • Emília Pozsgai and
  • Gyula Vatai
  • + 1 author

Enzymatic hydrolysis of soybean milk proteins with cysteine protease papain was performed in an advanced bioreactor, operated with batch mode. In soybean milk protein hydrolysis reaction, enzyme and substrate ratio and reaction temperature were varie...

  • Article
  • Open Access
24 Citations
6,395 Views
13 Pages

Enhancing the Antioxidant Ability of Trametes versicolor Polysaccharopeptides by an Enzymatic Hydrolysis Process

  • Mei-Hsin Jhan,
  • Ching-Hua Yeh,
  • Chia-Chun Tsai,
  • Ching-Tian Kao,
  • Chao-Kai Chang and
  • Chang-Wei Hsieh

10 September 2016

Polysaccharopeptides (PSPs) are among the main bioactive constituents of Trametes versicolor (T. versicolor). The purpose of this research was to investigate the antioxidant activities of enzymatic hydrolysates obtained from T. versicolor polysacchar...

  • Review
  • Open Access
12 Citations
4,232 Views
21 Pages

31 December 2023

Currently, the antioxidant properties of amino acids and their role in the physicochemical processes accompanying oxidative stress in cancer remain unclear. Cancer cells are known to extensively uptake amino acids, which are used as an energy source,...

  • Article
  • Open Access
2 Citations
1,358 Views
13 Pages

Isoquercitrin, a monoglucoside form of quercetin, exhibits superior antioxidant, anti-inflammatory, and cardiovascular protective effects in comparison to its precursor, rutin. However, its natural abundance is limited. This study aimed to increase t...

  • Article
  • Open Access
17 Citations
2,869 Views
13 Pages

15 June 2022

The effects of microwave pretreatment on the antioxidant activity and stability of enzymatic products from milk protein (MP) were studied. The peptide content, molecular weight distribution, and amino acid composition of MP hydrolysate were also meas...

  • Article
  • Open Access
40 Citations
4,392 Views
18 Pages

28 July 2021

Enzymatic pretreatment of seeds is a novel approach that enhances the health benefits of the extracted oil. The study investigated the influence of the enzymatic pretreatment of seeds on the quality of oil from different pomegranate cultivars. The qu...

  • Article
  • Open Access
1,174 Views
19 Pages

8 April 2025

The high-value utilization of enzymatic hydrolysis lignin (EHL) is essential for promoting the development of the biorefinery industry. This study investigated the enhancement of the antioxidant activity and ultraviolet (UV) absorption capacity of EH...

  • Article
  • Open Access
79 Citations
7,790 Views
15 Pages

Background: β-lactoglobulin hydrolysates (BLGH) have shown antioxidant, antihypertensive, antimicrobial, and opioid activity. In the current study, an innovative combination of high hydrostatic pressure and enzymatic hydrolysis (HHP–EH) was used to i...

  • Article
  • Open Access
12 Citations
4,524 Views
18 Pages

Synergistic Effect of L-Carnosine and Hyaluronic Acid in Their Covalent Conjugates on the Antioxidant Abilities and the Mutual Defense against Enzymatic Degradation

  • Valeria Lanza,
  • Valentina Greco,
  • Eleonora Bocchieri,
  • Sebastiano Sciuto,
  • Rosanna Inturri,
  • Luciano Messina,
  • Susanna Vaccaro,
  • Francesco Bellia and
  • Enrico Rizzarelli

Hyaluronic acid (Hy) is a natural linear polymer that is widely distributed in different organisms, especially in the articular cartilage and the synovial fluid. During tissue injury due to oxidative stress, Hy plays an important protective role. All...

  • Article
  • Open Access
47 Citations
9,488 Views
18 Pages

31 July 2021

Pumpkin seed oil is a by-product, abundant in nutrients and bioactive components that promote several health benefits. This study aimed to compare chemical compositions, antioxidant, and pharmacological activities of pumpkin seed oils extracted from...

  • Article
  • Open Access
40 Citations
6,370 Views
17 Pages

Seaweed oligosaccharides have attracted attention in food, agricultural, and medical applications recently. Compared to red and brown seaweeds, fewer studies have focused on the biological activity of green seaweed’s oligosaccharides. This study aime...

  • Article
  • Open Access
1,449 Views
17 Pages

30 April 2025

Cyanidin-3-O-glucoside (C3G) was used as a substrate for enzymatic acylation, and different compounds (methyl n-octanoate and methyl salicylate) were selected as acyl donors. Structural analysis (UV–Vis, FTIR, and HPLC) revealed the successful...

  • Article
  • Open Access
62 Citations
9,826 Views
12 Pages

Effects of Enzymatic Hydrolysis on the Functional Properties, Antioxidant Activity and Protein Structure of Black Soldier Fly (Hermetia illucens) Protein

  • Inayat Batish,
  • Devon Brits,
  • Pedro Valencia,
  • Caio Miyai,
  • Shamil Rafeeq,
  • Yixiang Xu,
  • Magdalini Galanopoulos,
  • Edward Sismour and
  • Reza Ovissipour

9 December 2020

The effects of chemical protein extraction, and enzymatic hydrolysis with Alcalase, papain and pepsin, on the functional properties, antioxidant activity, amino acid composition and protein structure of black soldier fly (H. illucens) larval protein...

  • Article
  • Open Access
42 Citations
9,117 Views
18 Pages

17 July 2020

Mushrooms are attractive resources for novel enzymes and bioactive compounds. Nevertheless, mushrooms spontaneously form brown pigments during food processing as well as extraction procedures for functional compounds. In this study, the dark browning...

  • Article
  • Open Access
10 Citations
3,218 Views
17 Pages

17 April 2024

Palmaria palmata is a viable source of nutrients with bioactive properties. The present study determined the potential role of post-extraction ultrasonication on some compositional features and antioxidant properties of enzymatic/alkaline extracts of...

  • Article
  • Open Access
9 Citations
6,767 Views
17 Pages

N-Acetyl Cysteine Restores the Diminished Activity of the Antioxidant Enzymatic System Caused by SARS-CoV-2 Infection: Preliminary Findings

  • María Elena Soto,
  • Linaloe Manzano-Pech,
  • Adrían Palacios-Chavarría,
  • Rafael Ricardo Valdez-Vázquez,
  • Verónica Guarner-Lans and
  • Israel Pérez-Torres

14 April 2023

SARS-CoV-2 infects type II pneumocytes and disrupts redox homeostasis by overproducing reactive oxygen species (ROS). N-acetyl cysteine (NAC) is a precursor of the synthesis of glutathione (GSH) and it restores the loss of redox homeostasis associate...

  • Article
  • Open Access
36 Citations
4,905 Views
10 Pages

24 April 2022

The effects of edible chitosan coating (0.1%, 0.3%, 0.5% and 0.75% w/v) on the changes in the quality, respiration rate, total phenolic content and anthocyanin of postharvest sweet cherry (Prunus avium L.) at 10 °C were investigated. The activiti...

  • Article
  • Open Access
14 Citations
4,418 Views
15 Pages

In this research, we aimed to compare the biological activities related to cosmeceutical applications of glutinous rice husk extracted by aqueous enzymatic extraction (AEE) and conventional solvent extraction. Cellulase enzymes were used to assist th...

  • Article
  • Open Access
34 Citations
6,489 Views
21 Pages

Chemical Structure–Antioxidant Activity Relationship of Water–Based Enzymatic Polymerized Rutin and Its Wound Healing Potential

  • Tanja Pivec,
  • Rupert Kargl,
  • Uroš Maver,
  • Matej Bračič,
  • Thomas Elschner,
  • Ema Žagar,
  • Lidija Gradišnik and
  • Karin Stana Kleinschek

26 September 2019

The flavonoid rutin (RU) is a known antioxidant substance of plant origin. Its potential application in pharmaceutical and cosmetic fields is, however, limited, due to its low water solubility. This limitation can be overcome by polymerization of the...

  • Article
  • Open Access
33 Citations
6,056 Views
23 Pages

7 December 2021

The essential oil industry of aromatic herbs and spices is currently producing a significant amount of by-products, such as the spent plant materials remaining after steam or hydrodistillation, that are simply discarded. The aim of this study was to...

  • Article
  • Open Access
12 Citations
3,353 Views
13 Pages

13 August 2021

Vinyl stearate was added to enzymatic transesterification of (−)-Epigallocatechin-3-O-gallate (EGCG) to enhance its lipophilicity and antioxidant ability in a non-aqueous system. The lipase DF “Amano” 15 was used as the catalyst. The optimal reaction...

  • Article
  • Open Access
13 Citations
3,210 Views
23 Pages

Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis

  • Mousa Alreshidi,
  • Mohammad A. Abdulhakeem,
  • Riadh Badraoui,
  • Giuseppe Amato,
  • Lucia Caputo,
  • Laura De Martino,
  • Filomena Nazzaro,
  • Florinda Fratianni,
  • Carmen Formisano and
  • Mejdi Snoussi
  • + 1 author

5 November 2023

Plants with medicinal benefits are a crucial source of compounds for developing drugs. This study was designed to determine the chemical composition, antibacterial, antibiofilm, antioxidant, and anti-enzymatic activities of Pulicaria incisa (Lam.) DC...

  • Article
  • Open Access
1 Citations
748 Views
16 Pages

Chemical Profile and Evaluation of the Antioxidant, Anti-Enzymatic, and Antibacterial Activity of Astragalus strictispinus and Astragalus macrocephalus subsp. finitimus

  • Saba Shahrivari-Baviloliaei,
  • Ilkay Erdogan Orhan,
  • Fatma Sezer Senol Deniz,
  • Mustafa Abdullah Yilmaz,
  • Agnieszka Viapiana,
  • Agnieszka Konopacka,
  • Osman Tugay and
  • Alina Plenis

15 November 2025

Astragalus species are characterized by rich active compounds, mainly polysaccharides, saponins, and polyphenols, with various important bioactivities, such as antioxidant, antitumor, anti-diabetes, antiviral, etc. In this study, the chemical profile...

  • Article
  • Open Access
23 Citations
4,384 Views
15 Pages

Antioxidant Capacity and Cytotoxic Effects of Catechins and Resveratrol Oligomers Produced by Enzymatic Oxidation against T24 Human Urinary Bladder Cancer Cells

  • Claudia Lizet Meneses-Gutiérrez,
  • Jacqueline Hernández-Damián,
  • José Pedraza-Chaverri,
  • Isabel Guerrero-Legarreta,
  • Dario Iker Téllez and
  • María Eugenia Jaramillo-Flores

In this work the polymerization of catechin, epicatechin, and resveratrol was carried out through a peroxidase oxidation process in order to improve the biological activity of these phenolic compounds. The antioxidant activity of the oligomers was ev...

  • Article
  • Open Access
249 Views
30 Pages

Biopolymer Development from Agro-Food and Aquaculture By-Products with Antioxidant Hydrolysates of Cyprinus carpio, Produced via Enzymatic Preparations of Pineapple and Papaya

  • Guadalupe López-García,
  • Octavio Dublán-García,
  • Francisco Antonio López-Medina,
  • Ana Gabriela Morachis-Valdez,
  • Karinne Saucedo-Vence,
  • Daniel Arizmendi-Cotero,
  • Daniel Díaz-Bandera,
  • Gerardo Heredia-García,
  • Angel Santillán-Álvarez and
  • Baciliza Quintero-Salazar
  • + 1 author

23 December 2025

This study describes the development of a biodegradable biopolymer formulated from protein–polysaccharide matrices enriched with antioxidant hydrolysates obtained from Cyprinus carpio by-products. The hydrolysates were produced through targeted...

  • Article
  • Open Access
54 Citations
7,738 Views
17 Pages

Stems are the important residues of Trapa quadrispinosa Roxb., which are abundant in phenolic compounds. Ultrasonic-assisted enzymatic extraction (UAEE) is confirmed as a novel extraction technology with main advantages of enhancing extraction yield...

  • Article
  • Open Access
2 Citations
3,274 Views
12 Pages

Cross-Sectional Study on the Association between Dietary Non-Enzymatic Antioxidant Capacity and Serum Liver Enzymes: The Furukawa Nutrition and Health Study

  • Hinako Nanri,
  • Ikuko Kashino,
  • Takeshi Kochi,
  • Masafumi Eguchi,
  • Shamima Akter,
  • Akiko Nanri,
  • Isamu Kabe and
  • Tetsuya Mizoue

10 July 2020

We examined the association of dietary non-enzymatic antioxidant capacity (NEAC) in overall diet, and separately from foods and beverages, with serum liver enzymes in a Japanese working population. This cross-sectional study was conducted among 1791...

  • Article
  • Open Access
49 Citations
7,045 Views
21 Pages

5 January 2023

In the current study, bighead carp fish were used in conjunction with the flavourzyme enzyme to obtain (FPH) fish protein hydrolysates. The optimum conditions of the hydrolysis process included an enzyme/substrate ratio of 4% and a temperature of 50...

  • Article
  • Open Access
10 Citations
3,543 Views
14 Pages

26 January 2022

Elaeagnus mollis oil extracted from the nuts of Elaeagnus mollis Diels can be used in food and pharmaceutical applications due to its excellent nutritional value. An ultrasound-assisted solvent enzymatic extraction (UASEE) method was used to extract...

  • Article
  • Open Access
Foods2026, 15(2), 392;https://doi.org/10.3390/foods15020392 
(registering DOI)

22 January 2026

The pistachio nut (Pistacia vera L.) is a rich and high-quality source of protein, as is its waste. This study investigated the potentials of pistachio nut waste proteins to obtain bioactive peptides exhibiting antioxidative and antibacterial activit...

  • Article
  • Open Access
667 Views
14 Pages

Effects of Seaweed Polysaccharide (SP) and Seaweed Enzymatic Hydrolysate (SEH) on Growth Performance, Antioxidant Capacity, Immune Function, and Gut Microbiota in Muscovy Ducks

  • Hong-Yan Wu,
  • Xiao-Feng Lin,
  • Chang-Sheng Fu,
  • Yang Yang,
  • Lei Wang,
  • Hai-Yan Wu,
  • Pan-Pan Guo,
  • Deng-Feng Wang and
  • Guang-Wen Yin

20 October 2025

This experiment investigated the effects of seaweed polysaccharide (SP) and seaweed enzymatic hydrolysate (SEH) on the growth performance, serum biochemical indices, antioxidant capacity, and intestinal function of Muscovy ducks. A total of 240 healt...

  • Article
  • Open Access
24 Citations
2,665 Views
12 Pages

8 December 2022

This study appraised the impact of enzymatic cottonseed protein concentrate (ECP) as a fish meal (FM) substitute on the growth and health of largemouth bass (Micropterus salmoides) (initial weight 14.99 ± 0.03 g). Five diets with equal nitroge...

  • Article
  • Open Access
25 Citations
3,900 Views
17 Pages

12 May 2021

Gracilariopsis lemaneiformis polysaccharides (GLP) were degraded using pectinase, glucoamylase, cellulase, xylanase, and β-dextranase into low-molecular-weight polysaccharides, namely, GPP, GGP, GCP, GXP, and GDP, respectively, and their antioxidant...

  • Article
  • Open Access
1 Citations
1,418 Views
19 Pages

Bioactive Properties of Enzymatically Hydrolyzed Mulberry Leaf Proteins: Antioxidant and Anti-Inflammatory Effects

  • Yichen Zhou,
  • Tianxu Liu,
  • Rijun Zhang,
  • Junyong Wang,
  • Jing Zhang,
  • Yucui Tong,
  • Haosen Zhang,
  • Zhenzhen Li,
  • Dayong Si and
  • Xubiao Wei

Oxidative stress and inflammatory responses often occur concomitantly, and they are key causative factors in various human and animal diseases. Evidence suggests that mulberry leaf protein (MLP) may have potential antioxidant and anti-inflammatory pr...

  • Article
  • Open Access
24 Citations
3,639 Views
14 Pages

The Enzymatic and Non-Enzymatic Antioxidant System Response of the Seagrass Cymodocea nodosa to Bisphenol-A Toxicity

  • Paraskevi Malea,
  • Danae Kokkinidi,
  • Alkistis Kevrekidou and
  • Ioannis-Dimosthenis S. Adamakis

25 January 2022

The effects of environmentally relevant bisphenol A (BPA) concentrations (0.3, 1 and 3 μg L−1) were tested at 2, 4, 6 and 8 days, on intermediate leaves, of the seagrass Cymodocea nodosa. Hydrogen peroxide (H2O2) production, lipid peroxidati...

  • Article
  • Open Access
10 Citations
4,411 Views
13 Pages

17 December 2023

In this study, the structure of the anthocyanin fractions isolated from black rice (Oryza sativa L.) was modified by the enzyme catalysis method using caffeic acid as an acyl donor. At the same time, the effects of the acylation on the lipophilicity,...

  • Review
  • Open Access
23 Citations
12,948 Views
23 Pages

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants

  • Valeria Pasciu,
  • Maria Nieddu,
  • Francesca Daniela Sotgiu,
  • Elena Baralla and
  • Fiammetta Berlinguer

13 July 2023

The present review aims to provide an overview of the assay methods for the quantification of ROS and principal enzymatic antioxidants as biomarkers of oxidative stress in erythrocytes and spermatozoa of small domestic ruminants. A complete literatur...

  • Article
  • Open Access
12 Citations
4,756 Views
18 Pages

Identification and Evaluation of Antioxidant and Anti-Aging Peptide Fractions from Enzymatically Hydrolyzed Proteins of Spirulina platensis and Chlorella vulgaris

  • Baran Masoumifeshani,
  • Abdolmohammad Abedian Kenari,
  • Ignacio Sottorff,
  • Max Crüsemann and
  • Jamshid Amiri Moghaddam

8 April 2025

Microalgae are a promising source of bioactive compounds, particularly proteins and peptides, with potential applications in skin health and the cosmetic industry. This study investigated the antioxidant and anti-aging properties of peptide fractions...

  • Article
  • Open Access
15 Citations
3,218 Views
16 Pages

Acid Treatment Enhances the Antioxidant Activity of Enzymatically Synthesized Phenolic Polymers

  • Maria Laura Alfieri,
  • Federica Moccia,
  • Gerardino D’Errico,
  • Lucia Panzella,
  • Marco d’Ischia and
  • Alessandra Napolitano

30 October 2020

Phenolic polymers produced by enzymatic oxidation under biomimetic and eco-friendly reaction conditions are usually endowed with potent antioxidant properties. These properties, coupled with the higher biocompatibility, stability and processability c...

  • Article
  • Open Access
12 Citations
3,919 Views
14 Pages

Selective Enzymatic Esterification of Lignin-Derived Phenolics for the Synthesis of Lipophilic Antioxidants

  • Marta Martinez-Garcia,
  • Jaime Gracia-Vitoria,
  • Karolien Vanbroekhoven,
  • Winnie Dejonghe and
  • Yamini Satyawali

Lignin is an abundant and renewable source of phenolic compounds that can be used as natural antioxidants to substitute synthetic, petroleum-based alternatives. The development of lignin depolymerization techniques has improved the accessibility of l...

  • Article
  • Open Access
103 Citations
10,259 Views
15 Pages

1 November 2012

Tilapia frame protein was hydrolyzed by different proteases, including properase E, pepsin, trypsin, flavourzyme, neutrase, gc106 and papain, to obtain antioxidant peptides. The tilapia frame protein hydrolysate (TFPH) obtained by trypsin exhibited t...

  • Article
  • Open Access
41 Citations
9,422 Views
25 Pages

6 March 2023

The aim of this research was to identify and quantify biologically active compounds from avocado (Persea americana L.) seeds (AS) utilizing different techniques with the use of ultrasound (US), ethanol (EtOH), and supercritical carbon dioxide (scCO2)...

  • Article
  • Open Access
80 Citations
9,899 Views
14 Pages

19 September 2022

Soybean protein hydrolysates were prepared using two proteolytic enzymes (Alcalase and Protamex) and the degree of hydrolysis (DH) and their functional and antioxidant properties were evaluated. The highest DH value was 20%, with a yield of 19.77% an...

  • Proceeding Paper
  • Open Access
2 Citations
2,939 Views
6 Pages

The aim of the work was the enzymatic synthesis of flavours and fragrances, antioxidants and antimicrobials with the use of benzyl alcohol and its selected derivatives via transesterification with vinyl acetate. The conversion yields of esters were d...

  • Article
  • Open Access
9 Citations
3,596 Views
20 Pages

The aim of this study was to evaluate the non-enzymatic and enzymatic antioxidant response of fifteen Hypericum perforatum L. dark-grown (HR1 A-HR1 O) and photoperiod-exposed (HR2 A-HR2 O) hairy root clones. Dark-grown HR1 clones were characterized w...

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