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  • Article
  • Open Access
3 Citations
2,101 Views
13 Pages

Molecular Characterization and Phylogenetic Analysis of Honeybee (Apis mellifera) Mite-Borne Pathogen DWV-A and DWV-B Isolated from Lithuania

  • Paulina Amšiejūtė-Graziani,
  • Vaclovas Jurgelevičius,
  • Simona Pilevičienė,
  • Žygimantas Janeliūnas and ,
  • Jana Radzijevskaja,
  • Algimantas Paulauskas,
  • Česlova Butrimaitė-Ambrozevičienė and
  • Ingrida Jacevičienė
  • + 3 authors+ 0 author

Deformed wing virus (DWV) is known as one of the main viruses that affect honeybees’ health all around the world. The virus has two widespread genotypes, DWV-A and DWV-B (VDV-1), transmitted mainly by V. destructor mites. In this study, we coll...

(This article belongs to the Special Issue Animal Virology, Molecular Diagnostics and Vaccine Development)
  • Article
  • Open Access
96 Citations
10,613 Views
12 Pages

DWV-A Lethal to Honey Bees (Apis mellifera): A Colony Level Survey of DWV Variants (A, B, and C) in England, Wales, and 32 States across the US

  • Jessica L. Kevill,
  • Flaviane S. de Souza,
  • Christopher Sharples,
  • Randy Oliver and ,
  • Declan C. Schroeder and
  • Stephen J. Martin
  • + 1 author+ 0 author

9 May 2019

The strong association between Varroa destructor, deformed wing virus (DWV), and high overwintering colony losses (OCL) of honey bees is well established. Three DWV master variants (DWV-A, -B, and -C) have been described, and their role in colony mor...

(This article belongs to the Special Issue Insect Viruses and Pest Management)
  • Article
  • Open Access
28 Citations
6,605 Views
11 Pages

24 May 2021

The combination of Deformed wing virus (DWV) and Varroa destructor is arguably one of the greatest threats currently facing western honey bees, Apis mellifera. Varroa’s association with DWV has decreased viral diversity and increased loads of DW...

(This article belongs to the Special Issue Viruses in Mass-Reared Invertebrates)
  • Article
  • Open Access
2,245 Views
20 Pages

Deformed wing virus (DWV) is a major contributor to honey bee colony losses, making effective monitoring essential for apiary management. Traditional DWV detection relies on hazardous RNA extraction followed by RT-qPCR, creating barriers for widespre...

  • Article
  • Open Access
10 Citations
3,894 Views
13 Pages

Interaction between Thiamethoxam and Deformed Wing Virus Type A on Wing Characteristics and Expression of Immune and Apoptosis Genes in Apis mellifera

  • Patcharin Phokasem,
  • Wannapha Mookhploy,
  • Sasiprapa Krongdang,
  • Chainarong Sinpoo and
  • Panuwan Chantawannakul

31 May 2022

Honey bees are economically important insects for crop pollination. They play a significant role as pollinators of wild plants and agricultural crops and produce economical products, such as honey, royal jelly, wax, pollen, propolis, and venom. Despi...

(This article belongs to the Special Issue Impact of Environmental Factors and Management Practices on Bee Health)
  • Article
  • Open Access
40 Citations
7,417 Views
16 Pages

Honey Bee Virus Transmission via Hive Products

  • Dominik Schittny,
  • Orlando Yañez and
  • Peter Neumann

The global trade of honey bee hive products has raised concern about pathogen transmission. However, the efficacy of hive products as virus vehicles is poorly understood. Here, we investigated the transmission capacity of hive products for Deformed w...

(This article belongs to the Special Issue Honey Bee Health)
  • Article
  • Open Access
18 Citations
4,173 Views
16 Pages

1 October 2021

Insects have a highly sensitive sense of smell, allowing them to perform complex behaviors, such as foraging and peer recognition. Their sense of smell is based on the recognition of ligands and is mainly coordinated by odorant-binding proteins (OBPs...

(This article belongs to the Section Insect Behavior and Pathology)
  • Article
  • Open Access
7 Citations
4,027 Views
15 Pages

Nursing Honeybee Behavior and Sensorial-Related Genes Are Altered by Deformed Wing Virus Variant A

  • Silva Diego,
  • Arismendi Nolberto,
  • Alveal Juan Pablo,
  • Ceballos Ricardo and ,
  • Zapata Nelson and
  • Vargas Marisol
  • + 1 author+ 0 author

23 January 2024

Insect behavior is coordinated mainly by smell through the diverse odor-binding proteins (OBP) that allow them to identify and recognize their environment. Sensory information collected through smell is then analyzed and interpreted in the brain, all...

  • Article
  • Open Access
7 Citations
3,114 Views
10 Pages

23 March 2024

The most prevalent viral pathogen of honeybees is Deformed Wing Virus (DWV) and its two most widely studied and common master-variants are DWV-A and DWV-B. The prevalence of DWV variants in the UK and in the US is changing, with the prevalence of the...

(This article belongs to the Section Insect Behavior and Pathology)
  • Article
  • Open Access
2 Citations
4,872 Views
12 Pages

18 January 2023

The invasion of Apis florea in Taiwan was first recorded in 2017. The deformed wing virus (DWV) has been identified as a common bee virus in apiculture around the world. Ectoparasitic mites are the main DWV vector for horizontal transmission. However...

(This article belongs to the Topic Advanced in Honey Bee and Apitherapy)
  • Article
  • Open Access
4 Citations
2,631 Views
28 Pages

Processing of the 3C/D Region of the Deformed Wing Virus (DWV)

  • Carina Maria Reuscher,
  • Sandra Barth,
  • Fiona Gockel,
  • Anette Netsch and ,
  • Kerstin Seitz,
  • Till Rümenapf and
  • Benjamin Lamp
  • + 2 authors+ 0 author

29 November 2023

The deformed wing virus (DWV) belongs to the genus Iflavirus and the family Iflaviridae within the order Picornavirales. It is an important pathogen of the Western honey bee, Apis mellifera, causing major losses among honey bee colonies in associatio...

(This article belongs to the Section Invertebrate Viruses)
  • Article
  • Open Access
5 Citations
2,941 Views
13 Pages

1 October 2023

Deformed wing virus (DWV) is one of the important pathogens of the honey bee (Apis mellifera), which consists of three master variants: types A, B, and C. Among them, DWV types A (DWV-A) and B (DWV-B) are the most prevalent variants in honey bee colo...

(This article belongs to the Section Invertebrate Viruses)
  • Article
  • Open Access
2 Citations
3,899 Views
24 Pages

Characterization of a Molecular Clone of Deformed Wing Virus B

  • Sandra Barth,
  • Sebastian Affeldt,
  • Claudia Blaurock,
  • Irmin Lobedank and ,
  • Anette Netsch,
  • Kerstin Seitz,
  • Till Rümenapf and
  • Benjamin Lamp
  • + 3 authors+ 0 author

18 June 2024

Honey bees (Apis mellifera) play a crucial role in agriculture through their pollination activities. However, they have faced significant health challenges over the past decades that can limit colony performance and even lead to collapse. A primary c...

(This article belongs to the Section Invertebrate Viruses)
  • Article
  • Open Access
12 Citations
4,625 Views
9 Pages

4 June 2021

Viruses, and in particular the deformed wing virus (DWV), are considered as one of the main antagonists of honey bee health. The ‘suppressed in ovo virus infection’ trait (SOV) described for the first time that control of a virus infection can be ach...

(This article belongs to the Special Issue Insect Viruses and Pest Management 2.0)
  • Article
  • Open Access
81 Citations
10,531 Views
18 Pages

The Two Prevalent Genotypes of an Emerging Infectious Disease, Deformed Wing Virus, Cause Equally Low Pupal Mortality and Equally High Wing Deformities in Host Honey Bees

  • Anja Tehel,
  • Quynh Vu,
  • Diane Bigot,
  • Andreas Gogol-Döring and ,
  • Peter Koch,
  • Christina Jenkins,
  • Vincent Doublet,
  • Panagiotis Theodorou and
  • Robert Paxton
  • + 4 authors+ 0 author

29 January 2019

Deformed wing virus (DWV) is an emerging infectious disease of the honey bee (Apis mellifera) that is considered a major cause of elevated losses of honey bee colonies. DWV comprises two widespread genotypes: the originally described genotype A, and...

(This article belongs to the Special Issue Emerging Viruses)
  • Article
  • Open Access
2 Citations
1,139 Views
6 Pages

Viruses Infecting Cuban Honey Bees and Evolution of Deformed-Wing-Virus Variants

  • Poppy J. Hesketh-Best,
  • Anais R. Luis,
  • Declan C. Schroeder and
  • Stephen J. Martin

22 January 2026

Cuba is in a unique situation in which it has a large (220,000 managed colonies) and isolated honey bee population due to a 60+ year ban on the importation of bees. Despite this, the ectoparasitic mite Varroa destructor arrived in 1996, and with it c...

(This article belongs to the Section Invertebrate Viruses)
  • Article
  • Open Access
11 Citations
4,599 Views
15 Pages

Effects of Varroa destructor on Hemolymph Sugars and Secondary Infections in Honeybees (Apis mellifera)

  • Antoine Cournoyer,
  • Laurence Plamondon,
  • Liza Bau-Gaudreault,
  • Annie Deschamps and ,
  • Pascal Dubreuil and
  • Marie-Odile Benoit-Biancamano
  • + 1 author+ 0 author

16 November 2022

The European honeybee contributes to the agriculture by its pollination; however, the overwintering loss rate over the last decades is worrisome. Varroa destructor is considered one of the most important causes of bee colony declines. This project ai...

(This article belongs to the Special Issue Frontier Research in Apiculture (Diagnosis and Control of Bee Diseases, Bee Products, Environmental Monitoring))
  • Article
  • Open Access
10 Citations
3,005 Views
12 Pages

First Detection of Honeybee Pathogenic Viruses in Butterflies

  • Metka Pislak Ocepek,
  • Gordana Glavan,
  • Rudi Verovnik,
  • Laura Šimenc and
  • Ivan Toplak

12 October 2022

Several pathogens are important causes of the observed pollinator decline, some of which could be transmitted between different pollinator species. To determine whether honeybee viruses can be transmitted to butterflies, a total of 120 butterflies we...

(This article belongs to the Section Insect Behavior and Pathology)
  • Article
  • Open Access
953 Views
16 Pages

Distribution and Quantification of Infectious and Parasitic Agents in Managed Honeybees in Central Italy, the Republic of Kosovo, and Albania

  • Franca Rossi,
  • Martina Iannitto,
  • Beqe Hulaj,
  • Luciano Ricchiuti and ,
  • Ani Vodica,
  • Patrizia Tucci,
  • Franco Mutinelli and
  • Anna Granato
  • + 3 authors+ 0 author

This study aimed to determine the presence of relevant infectious and parasitic agents (IPAs) in managed honeybees from Central Italy and the Republic of Kosovo and Albania to assess the overall health status of local apiaries by determining the cont...

(This article belongs to the Special Issue Infectious Diseases in Animals)
  • Article
  • Open Access
8 Citations
5,267 Views
12 Pages

Nationwide Screening for Bee Viruses in Apis mellifera Colonies in Egypt

  • Mohamed Kandel,
  • Robert J. Paxton and
  • Yahya Al Naggar

9 February 2023

Honey bees are essential for crop and wild plant pollination. However, many countries have reported high annual colony losses caused by multiple possible stressors. Diseases, particularly those caused by viruses, are a major cause of colony losses. H...

(This article belongs to the Section Social Insects and Apiculture)
  • Article
  • Open Access
5 Citations
2,605 Views
12 Pages

Verifying the inclusivity of molecular detection methods gives indications about the reliability of viral infection diagnosis because of the tendency of viral pathogens to undergo sequence variation. This study was aimed at selecting inclusive probes...

(This article belongs to the Section Microbial Biotechnology)
  • Article
  • Open Access
19 Citations
6,176 Views
12 Pages

Nationwide Screening for Bee Viruses and Parasites in Belgian Honey Bees

  • Severine Matthijs,
  • Valérie De Waele,
  • Valerie Vandenberge,
  • Bénédicte Verhoeven and ,
  • Jacqueline Evers,
  • Marleen Brunain,
  • Claude Saegerman,
  • Paul J. J. De Winter,
  • Stefan Roels and
  • Nick De Regge
  • + 6 authors+ 1 author

14 August 2020

The health of honey bees is threatened by multiple factors, including viruses and parasites. We screened 557 honey bee (Apis mellifera) colonies from 155 beekeepers distributed all over Belgium to determine the prevalence of seven widespread viruses...

(This article belongs to the Special Issue Novel Concepts in Virology)
  • Article
  • Open Access
5 Citations
4,770 Views
12 Pages

Intra-Colonial Viral Infections in Western Honey Bees (Apis Mellifera)

  • Loreley Castelli,
  • María Laura Genchi García,
  • Anne Dalmon,
  • Daniela Arredondo and ,
  • Karina Antúnez,
  • Ciro Invernizzi,
  • Francisco José Reynaldi,
  • Yves Le Conte and
  • Alexis Beaurepaire
  • + 4 authors+ 0 author

RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies r...

(This article belongs to the Special Issue Microorganisms in Pollinators: Interactions with Other Factors)
  • Article
  • Open Access
36 Citations
7,009 Views
16 Pages

RNAseq Analysis Reveals Virus Diversity within Hawaiian Apiary Insect Communities

  • Laura E. Brettell,
  • Declan C. Schroeder and
  • Stephen J. Martin

27 April 2019

Deformed wing virus (DWV) is the most abundant viral pathogen of honey bees and has been associated with large-scale colony losses. DWV and other bee-associated RNA viruses are generalists capable of infecting diverse hosts. Here, we used RNAseq anal...

(This article belongs to the Special Issue Virus Bioinformatics)
  • Article
  • Open Access
51 Citations
5,679 Views
17 Pages

Presence of Known and Emerging Honey Bee Pathogens in Apiaries of Veneto Region (Northeast of Italy) during Spring 2020 and 2021

  • Fulvio Bordin,
  • Laura Zulian,
  • Anna Granato,
  • Mauro Caldon and ,
  • Rosa Colamonico,
  • Marica Toson,
  • Laura Trevisan,
  • Laura Biasion and
  • Franco Mutinelli
  • + 4 authors+ 0 author

18 February 2022

A progressive honey bee population decline has been reported worldwide during the last decades, and it could be attributed to several causes, in particular to the presence of pathogens and parasites that can act individually or in synergy. The health...

(This article belongs to the Special Issue Frontier Research in Apiculture (Diagnosis and Control of Bee Diseases, Bee Products, Environmental Monitoring))
  • Article
  • Open Access
21 Citations
7,529 Views
15 Pages

Impacts of Diverse Natural Products on Honey Bee Viral Loads and Health

  • Dawn L. Boncristiani,
  • James P. Tauber,
  • Evan C. Palmer-Young,
  • Lianfei Cao and ,
  • William Collins,
  • Kyle Grubbs,
  • Jillian A. Lopez,
  • Lyndel W. Meinhardt,
  • Vy Nguyen and
  • Jay D. Evans
  • + 9 authors+ 4 authors

13 November 2021

Western honey bees (Apis mellifera), a cornerstone to crop pollination in the U.S., are faced with an onslaught of challenges from diseases caused by parasites, pathogens, and pests that affect this economically valuable pollinator. Natural products...

(This article belongs to the Special Issue Frontier Research in Apiculture (Diagnosis and Control of Bee Diseases, Bee Products, Environmental Monitoring))
  • Article
  • Open Access
10 Citations
4,422 Views
18 Pages

Dietary Supplement of Grape Wastes Enhances Honeybee Immune System and Reduces Deformed Wing Virus (DWV) Load

  • Guillermo Pascual,
  • Diego Silva,
  • Marisol Vargas,
  • Mario Aranda and ,
  • Juan Antonio Cañumir and
  • María Dolores López
  • + 1 author+ 0 author

27 December 2022

Ingredients rich in phenolic compounds and antioxidants of winemaking wastes, which play an important role in the prevention of various diseases and the control of viruses, are being explored. Currently, there is a concern about honeybee population l...

(This article belongs to the Special Issue Agri-Food Wastes as Natural Source of Bioactive Antioxidants II)
  • Feature Paper
  • Article
  • Open Access
44 Citations
9,786 Views
15 Pages

Honey Bee Survival and Pathogen Prevalence: From the Perspective of Landscape and Exposure to Pesticides

  • Mohamed Alburaki,
  • Deniz Chen,
  • John A. Skinner,
  • William G. Meikle and ,
  • David R. Tarpy,
  • John Adamczyk and
  • Scott D. Stewart
  • + 2 authors+ 0 author

13 June 2018

In order to study the in situ effects of the agricultural landscape and exposure to pesticides on honey bee health, sixteen honey bee colonies were placed in four different agricultural landscapes. Those landscapes were three agricultural areas with...

(This article belongs to the Special Issue Stressors on Bee Health)
  • Article
  • Open Access
14 Citations
6,567 Views
14 Pages

The Pathogen Profile of a Honey Bee Queen Does Not Reflect That of Her Workers

  • Jessica L. Kevill,
  • Katie Lee,
  • Michael Goblirsch,
  • Erin McDermott and ,
  • David R. Tarpy,
  • Marla Spivak and
  • Declan C. Schroeder
  • + 2 authors+ 0 author

20 June 2020

Throughout a honey bee queen’s lifetime, she is tended to by her worker daughters, who feed and groom her. Such interactions provide possible horizontal transmission routes for pathogens from the workers to the queen, and as such a queen’...

  • Article
  • Open Access
27 Citations
5,570 Views
15 Pages

Foodborne Transmission and Clinical Symptoms of Honey Bee Viruses in Ants Lasius spp.

  • Daniel Schläppi,
  • Nor Chejanovsky,
  • Orlando Yañez and
  • Peter Neumann

17 March 2020

Emerging infectious diseases are often the products of host shifts, where a pathogen jumps from its original host to a novel species. Viruses in particular cross species barriers frequently. Acute bee paralysis virus (ABPV) and deformed wing virus (D...

(This article belongs to the Section Invertebrate Viruses)
  • Feature Paper
  • Article
  • Open Access
11 Citations
5,003 Views
12 Pages

Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in Solenopsis invicta Buren (Hymenoptera: Formicidae)

  • Godfrey P. Miles,
  • Xiaofen F. Liu,
  • Esmaeil Amiri,
  • Michael J. Grodowitz and ,
  • Margaret L. Allen and
  • Jian Chen
  • + 1 author+ 0 author

27 September 2023

Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasisp...

(This article belongs to the Collection Biology of Social Insect Diseases)
  • Article
  • Open Access
2 Citations
2,254 Views
12 Pages

Megaselia scalaris and Senotainia tricuspis Infesting Apis mellifera: Detection by Quantitative PCR, Genotyping, and Involvement in the Transmission of Microbial Pathogens

  • Franca Rossi,
  • Martina Iannitto,
  • Beqe Hulaj,
  • Paola Manocchio and ,
  • Francesca Gentile,
  • Ilaria Del Matto,
  • Massimiliano Paoletti,
  • Lucio Marino and
  • Luciano Ricchiuti
  • + 4 authors+ 0 author

9 October 2024

The Megaselia scalaris and Senotainia tricuspis parasitoid flies of the honeybee Apis mellifera were found to infest apiaries of different European and Mediterranean countries but their prevalence and impact on apiary health are little known. Therefo...

(This article belongs to the Section Insect Behavior and Pathology)
  • Article
  • Open Access
17 Citations
4,489 Views
10 Pages

Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)

  • Antonio Felicioli,
  • Mario Forzan,
  • Simona Sagona,
  • Paola D’Agostino and ,
  • Diego Baido,
  • Baldassare Fronte and
  • Maurizio Mazzei
  • + 2 authors+ 0 author

25 April 2020

Honeybee pathogens have an important role in honeybee colony mortality and colony losses; most of them are widely spread and necessitate worldwide solutions to contrast honeybee’s decline. Possible accepted solutions to cope with the spread of...

  • Article
  • Open Access
82 Citations
9,755 Views
14 Pages

ABC Assay: Method Development and Application to Quantify the Role of Three DWV Master Variants in Overwinter Colony Losses of European Honey Bees

  • Jessica L. Kevill,
  • Andrea Highfield,
  • Gideon J. Mordecai,
  • Stephen J. Martin and
  • Declan C. Schroeder

27 October 2017

Deformed wing virus (DWV) is one of the most prevalent honey bee viral pathogens in the world. Typical of many RNA viruses, DWV is a quasi-species, which is comprised of a large number of different variants, currently consisting of three master varia...

(This article belongs to the Special Issue Antiviral Defense in Invertebrates)
  • Abstract
  • Open Access
1 Citations
1,595 Views
1 Page

The ecological and economic importance of bees for pollination and biodiversity is well established. The health of bees is, however, threatened by a multitude of factors, including viruses. In this study, we screened 557 colonies from 155 beekeepers...

(This article belongs to the Proceedings of Viruses 2020—Novel Concepts in Virology)
  • Communication
  • Open Access
1 Citations
731 Views
8 Pages

First Detection of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) in Central Hungary in European Hornet (Vespa crabro Linnaeus, 1758)

  • János Gál,
  • Árisz Ziszisz,
  • Márton Hoitsy,
  • Míra Mándoki and ,
  • Krisztina Bali,
  • Lilla Dénes,
  • Enikő Fehér,
  • Ákos Jerzsele,
  • Gábor Halász and
  • Eszter Kaszab
  • + 5 authors+ 0 author

11 December 2025

This study aimed to investigate the presence of known bee viruses in the European hornet (Vespa crabro, Linnaeus, 1758), a species recognized as a bee predator in Hungary. Several viruses affecting honeybees (Apis mellifera, Linnaeus, 1758), such as...

  • Article
  • Open Access
32 Citations
4,929 Views
17 Pages

11 July 2021

The viral loads of acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Lake Sinai virus 3 (LSV3), and sacbrood bee virus (SBV) were determined in samples with the use of quan...

(This article belongs to the Section Invertebrate Viruses)