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Article

Intra-Colonial Viral Infections in Western Honey Bees (Apis Mellifera)

1
Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, Uruguay
2
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata 1900, Buenos Aires, Argentina
3
Instituto Multidisciplinario de Biología Celular (IMBICE), La Plata 1900, Buenos Aires, Argentina
4
Laboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (LAVIR-FCV-UNLP), La Plata 1900, Buenos Aires, Argentina
5
Abeilles et Environnement, INRAE, 84000 Avignon, France
6
Sección Etología, Facultad de Ciencias, Montevideo 11400, Uruguay
7
Institute of Bee Health, University of Bern, 3003 Bern, Switzerland
*
Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Academic Editor: Dániel Cadar
Microorganisms 2021, 9(5), 1087; https://doi.org/10.3390/microorganisms9051087
Received: 31 March 2021 / Revised: 1 May 2021 / Accepted: 11 May 2021 / Published: 18 May 2021
(This article belongs to the Special Issue Microorganisms in Pollinators: Interactions with Other Factors)
RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects. View Full-Text
Keywords: evolutionary biology; host–pathogen interactions; population genetics; viruses; pollinators evolutionary biology; host–pathogen interactions; population genetics; viruses; pollinators
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MDPI and ACS Style

Castelli, L.; Genchi García, M.L.; Dalmon, A.; Arredondo, D.; Antúnez, K.; Invernizzi, C.; Reynaldi, F.J.; Le Conte, Y.; Beaurepaire, A. Intra-Colonial Viral Infections in Western Honey Bees (Apis Mellifera). Microorganisms 2021, 9, 1087. https://doi.org/10.3390/microorganisms9051087

AMA Style

Castelli L, Genchi García ML, Dalmon A, Arredondo D, Antúnez K, Invernizzi C, Reynaldi FJ, Le Conte Y, Beaurepaire A. Intra-Colonial Viral Infections in Western Honey Bees (Apis Mellifera). Microorganisms. 2021; 9(5):1087. https://doi.org/10.3390/microorganisms9051087

Chicago/Turabian Style

Castelli, Loreley, María L. Genchi García, Anne Dalmon, Daniela Arredondo, Karina Antúnez, Ciro Invernizzi, Francisco J. Reynaldi, Yves Le Conte, and Alexis Beaurepaire. 2021. "Intra-Colonial Viral Infections in Western Honey Bees (Apis Mellifera)" Microorganisms 9, no. 5: 1087. https://doi.org/10.3390/microorganisms9051087

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