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Editor’s Choice Articles

Editor’s Choice articles are based on recommendations by the scientific editors of MDPI journals from around the world. Editors select a small number of articles recently published in the journal that they believe will be particularly interesting to readers, or important in the respective research area. The aim is to provide a snapshot of some of the most exciting work published in the various research areas of the journal.

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18 pages, 3245 KiB  
Article
Electrical Phenotyping of Aged Human Mesenchymal Stem Cells Using Dielectrophoresis
by Lexi L. C. Simpkins, Tunglin Tsai, Emmanuel Egun and Tayloria N. G. Adams
Micromachines 2025, 16(4), 435; https://doi.org/10.3390/mi16040435 - 3 Apr 2025
Viewed by 306
Abstract
Human mesenchymal stem cells (hMSCs) are widely used in regenerative medicine, but large-scale in vitro expansion alters their function, impacting proliferation and differentiation potential. Currently, a predictive marker to assess these changes is lacking. Here, we used dielectrophoresis (DEP) to characterize the electrical [...] Read more.
Human mesenchymal stem cells (hMSCs) are widely used in regenerative medicine, but large-scale in vitro expansion alters their function, impacting proliferation and differentiation potential. Currently, a predictive marker to assess these changes is lacking. Here, we used dielectrophoresis (DEP) to characterize the electrical phenotype of hMSCs derived from bone marrow (BM), adipose tissue (AT), and umbilical cord (UC) as they aged in vitro from passage 4 (P4) to passage 9 (P9). The electrical phenotype was defined by the DEP spectra, membrane capacitance, and cytoplasm conductivity. Cell morphology and size, growth characteristics, adipogenic differentiation potential, and osteogenic differentiation potential were assessed alongside label-free biomarker membrane capacitance and cytoplasm conductivity. Differentiation was confirmed by histological staining and RT-qPCR. All hMSCs exhibited typical morphology, though cell size varied, with UC-hMSCs displaying the largest variability across all size metrics. Growth analysis revealed that UC-hMSCs proliferated the fastest. The electrical phenotype varied with cell source and in vitro age, with high passage hMSCs showing noticeable shifts in DEP spectra, membrane capacitance, and cytoplasm conductivity. Correlation analysis revealed that population doubling level (PDL) correlated with membrane capacitance and cytoplasm conductivity, indicating PDL as a more precise marker of in vitro aging than passage number. Additionally, we demonstrate that membrane capacitance correlates with the osteogenic marker COL1A1 and that cytoplasm conductivity correlates with the adipogenic markers ADIPOQ and FABP4, suggesting that DEP-derived electrical properties serve as label-free biomarkers of differentiation potential. While DEP has previously been applied to BM-hMSCs and AT-hMSCs, and more recently to UC-hMSCs, few studies have provided a direct comparison across all three sources or tracked changes across continuous expansion. These findings underscore the utility of DEP as a label-free approach for assessing hMSC aging and function, offering practical applications for optimizing stem cell expansion and stem cell banking in clinical settings. Full article
(This article belongs to the Special Issue Micro/Nanotechnology for Cell Manipulation, Detection and Analysis)
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35 pages, 2334 KiB  
Review
Innovative Micro- and Nano-Architectures in Biomedical Engineering for Therapeutic and Diagnostic Applications
by Nargish Parvin, Sang Woo Joo, Jae Hak Jung and Tapas K. Mandal
Micromachines 2025, 16(4), 419; https://doi.org/10.3390/mi16040419 - 31 Mar 2025
Viewed by 478
Abstract
The rapid evolution of micro- and nano-architectures is revolutionizing biomedical engineering, particularly in the fields of therapeutic and diagnostic micromechanics. This review explores the recent innovations in micro- and nanostructured materials and their transformative impact on healthcare applications, ranging from drug delivery and [...] Read more.
The rapid evolution of micro- and nano-architectures is revolutionizing biomedical engineering, particularly in the fields of therapeutic and diagnostic micromechanics. This review explores the recent innovations in micro- and nanostructured materials and their transformative impact on healthcare applications, ranging from drug delivery and tissue engineering to biosensing and diagnostics. Key advances in fabrication techniques, such as lithography, 3D printing, and self-assembly, have enabled unprecedented control over material properties and functionalities at microscopic scales. These engineered architectures offer enhanced precision in targeting and controlled release in drug delivery, foster cellular interactions in tissue engineering, and improve sensitivity and specificity in diagnostic devices. We examine critical design parameters, including biocompatibility, mechanical resilience, and scalability, which influence their clinical efficacy and long-term stability. This review also highlights the translational potential and current limitations in bringing these materials from the laboratory research to practical applications. By providing a comprehensive overview of the current trends, challenges, and future perspectives, this article aims to inform and inspire further development in micro- and nano-architectures that hold promise for advancing personalized and precision medicine. Full article
(This article belongs to the Special Issue Emerging Trends in Biosensors and Biomolecular Electronics Towards Disease Progression and Treatment)
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27 pages, 2399 KiB  
Review
Carbon Materials in Voltammetry: An Overview of Versatile Platforms for Antidepressant Drug Detection
by Joanna Smajdor, Katarzyna Fendrych and Anna Górska-Ratusznik
Micromachines 2025, 16(4), 423; https://doi.org/10.3390/mi16040423 - 31 Mar 2025
Viewed by 274
Abstract
This review concentrates on the application of carbon-based materials in the development and fabrication of voltammetric sensors of antidepressant drugs used in the treatment of moderate to severe depression, anxiety disorders, personality disorders, and various phobias. Voltammetric techniques offer outstanding sensitivity and selectivity, [...] Read more.
This review concentrates on the application of carbon-based materials in the development and fabrication of voltammetric sensors of antidepressant drugs used in the treatment of moderate to severe depression, anxiety disorders, personality disorders, and various phobias. Voltammetric techniques offer outstanding sensitivity and selectivity, accuracy, low detection limit, high reproducibility, instrumental simplicity, cost-effectiveness, and short time of direct determination of antidepressant drugs in pharmaceutical and clinical samples. Moreover, the combination of voltammetric approaches with the unique characteristics of carbon and its derivatives has led to the development of powerful electrochemical sensing tools for detecting antidepressant drugs, which are highly desirable in healthcare, environmental monitoring, and the pharmaceutical industry. In this review, carbon-based materials, such as glassy carbon and boron-doped diamond, and a wide spectrum of carbon nanoparticles, including graphene, graphene oxides, reduced graphene oxides, single-walled carbon nanotubes, and multi-walled carbon nanotubes were described in terms of the sensing performance of agomelatine, alprazolam, amitriptyline, aripiprazole, carbamazepine, citalopram, clomipramine, clozapine, clonazepam, desipramine, desvenlafaxine, doxepin, duloxetine, flunitrazepam, fluoxetine, fluvoxamine, imipramine, nifedipine, olanzapine, opipramol, paroxetine, quetiapine, serotonin, sertraline, sulpiride, thioridazine, trazodone, venlafaxine, and vortioxetine. Full article
(This article belongs to the Special Issue Micro- and Nanosensors: Fabrication, Applications and Performance Enhancements, Third Edition)
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20 pages, 18426 KiB  
Review
Nanostructures and Nanomaterials Integrated into Triboelectric Nanogenerators
by Shujie Yang, Victor Klinkov, Natalia Grozova, Svetlana Shalnova, Tatiana Larionova, Oleg Tolochko and Olga Klimova-Korsmik
Micromachines 2025, 16(4), 403; https://doi.org/10.3390/mi16040403 - 29 Mar 2025
Viewed by 225
Abstract
The pursuit of eco-friendly and renewable power generation has driven technological breakthroughs in nanoscale engineering, particularly regarding triboelectric nanogenerators (TENGs). These devices have become a focus of interest due to their capacity to effectively transform kinetic energy into electrical power via combined triboelectrification [...] Read more.
The pursuit of eco-friendly and renewable power generation has driven technological breakthroughs in nanoscale engineering, particularly regarding triboelectric nanogenerators (TENGs). These devices have become a focus of interest due to their capacity to effectively transform kinetic energy into electrical power via combined triboelectrification and electrostatic charge separation mechanisms. TENGs now find expanding implementations across multiple fields including in flexible electronics, autonomous sensing systems, and ambient energy conversion technologies. Enhancing TENG performance critically depends on the strategic design and application of nanostructures and nanomaterials. Nonetheless, challenges such as material selection, compatibility, homogeneous dispersion, interfacial stability, and production scalability must be overcome to advance TENG technology. Moreover, the mechanisms by which nanomaterials contribute to the triboelectric effect remain insufficiently understood, underscoring the necessity for systematic theoretical models. This review provides a comprehensive overview of recent advancements in integrating nanostructures and nanomaterials into TENGs, elucidating their roles, advantages, and underlying mechanisms in enhancing energy conversion efficiency, while identifying key challenges and proposing future research directions. Full article
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13 pages, 10049 KiB  
Article
Rapid and Sensitive Detection of Thrombospondin-2 Using Nanoparticle Sensors for Cancer Screening and Prognosis
by Maziyar Kalateh Mohammadi, Seyedsina Mirjalili, Md Ashif Ikbal, Hao Xie and Chao Wang
Micromachines 2025, 16(3), 354; https://doi.org/10.3390/mi16030354 - 20 Mar 2025
Viewed by 439
Abstract
Thrombospondin-2 (THBS2) is a prevailing prognostic biomarker implicated in different cancer types, such as deadly colorectal, pancreas, and triple-negative breast cancers. While the current methods for cancer-relevant protein detection, such as enzyme-linked immunosorbent assay (ELISA), mass spectrometry, and immunohistochemistry, are feasible at advanced [...] Read more.
Thrombospondin-2 (THBS2) is a prevailing prognostic biomarker implicated in different cancer types, such as deadly colorectal, pancreas, and triple-negative breast cancers. While the current methods for cancer-relevant protein detection, such as enzyme-linked immunosorbent assay (ELISA), mass spectrometry, and immunohistochemistry, are feasible at advanced stages, they have shortcomings in sensitivity, specificity, and accessibility, particularly at low concentrations in complex biological fluids for early detection. Here, we propose and demonstrate a modular, in-solution assay design concept, Nanoparticle-Supported Rapid Electronic Detection (NasRED), as a versatile cancer screening and diagnostic platform. NasRED utilizes antibody-functionalized gold nanoparticles (AuNPs) to capture target proteins from a minute amount of sample (<10 µL) and achieve optimal performance with a short assay time by introducing active fluidic forces that act to promote biochemical reaction and accelerate signal transduction. This rapid (15 min) process serves to form AuNP clusters upon THBS2 binding and subsequently precipitate such clusters, resulting in color modulation of the test tubes that is dependent on the THBS2 concentration. Finally, a semiconductor-based, portable electronic device is used to digitize the optical signals for the sensitive detection of THBS2. High sensitivity (femtomolar level) and a large dynamic range (five orders of magnitude) are obtained to analyze THBS2 spiked in PBS, serum, whole blood, saliva, cerebrospinal fluids, and synovial fluids. High specificity is also preserved in differentiating THBS2 from other markers such as cancer antigen (CA) 19-9 and bovine serum albumin (BSA). This study highlights NasRED’s potential to enhance cancer prognosis and screening by offering a cost-effective, accessible, and minimally invasive solution. Full article
(This article belongs to the Special Issue Immunoassay Platforms for Biomedical Detection)
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16 pages, 3503 KiB  
Article
A Modular, Cost-Effective, and Pumpless Perfusion Assembly for the Long-Term Culture of Engineered Microvessels
by Shashwat S. Agarwal, Jacob C. Holter, Travis H. Jones, Brendan T. Fuller, Joseph W. Tinapple, Joseph M. Barlage and Jonathan W. Song
Micromachines 2025, 16(3), 351; https://doi.org/10.3390/mi16030351 - 19 Mar 2025
Viewed by 994
Abstract
Continuous perfusion is necessary to sustain microphysiological systems and other microfluidic cell cultures. However, most of the established microfluidic perfusion systems, such as syringe pumps, peristaltic pumps, and rocker plates, have several operational challenges and may be cost-prohibitive, especially for laboratories with no [...] Read more.
Continuous perfusion is necessary to sustain microphysiological systems and other microfluidic cell cultures. However, most of the established microfluidic perfusion systems, such as syringe pumps, peristaltic pumps, and rocker plates, have several operational challenges and may be cost-prohibitive, especially for laboratories with no microsystems engineering expertise. Here, we address the need for a cost-efficient, easy-to-implement, and reliable microfluidic perfusion system. Our solution is a modular pumpless perfusion assembly (PPA), which is constructed from commercially available, interchangeable, and aseptically packaged syringes and syringe filters. The total cost for the components of each assembled PPA is USD 1–2. The PPA retains the simplicity of gravity-based pumpless flow systems but incorporates high resistance filters that enable slow and sustained flow for extended periods of time (hours to days). The perfusion characteristics of the PPA were determined by theoretical calculations of the total hydraulic resistance of the assembly and experimental characterization of specific filter resistances. We demonstrated that the PPA enabled reliable long-term culture of engineered endothelialized 3-D microvessels for several weeks. Taken together, our novel PPA solution is simply constructed from extremely low-cost and commercially available laboratory supplies and facilitates robust cell culture and compatibility with current microfluidic setups. Full article
(This article belongs to the Special Issue Current Trends in Microfabrication Techniques for Lab-on-a-Chip and Biomedical Microdevices, 2nd Edition)
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32 pages, 17993 KiB  
Review
Design, Fabrication, and Application of Large-Area Flexible Pressure and Strain Sensor Arrays: A Review
by Xikuan Zhang, Jin Chai, Yongfu Zhan, Danfeng Cui, Xin Wang and Libo Gao
Micromachines 2025, 16(3), 330; https://doi.org/10.3390/mi16030330 - 12 Mar 2025
Viewed by 807
Abstract
The rapid development of flexible sensor technology has made flexible sensor arrays a key research area in various applications due to their exceptional flexibility, wearability, and large-area-sensing capabilities. These arrays can precisely monitor physical parameters like pressure and strain in complex environments, making [...] Read more.
The rapid development of flexible sensor technology has made flexible sensor arrays a key research area in various applications due to their exceptional flexibility, wearability, and large-area-sensing capabilities. These arrays can precisely monitor physical parameters like pressure and strain in complex environments, making them highly beneficial for sectors such as smart wearables, robotic tactile sensing, health monitoring, and flexible electronics. This paper reviews the fabrication processes, operational principles, and common materials used in flexible sensors, explores the application of different materials, and outlines two conventional preparation methods. It also presents real-world examples of large-area pressure and strain sensor arrays. Fabrication techniques include 3D printing, screen printing, laser etching, magnetron sputtering, and molding, each influencing sensor performance in different ways. Flexible sensors typically operate based on resistive and capacitive mechanisms, with their structural designs (e.g., sandwich and fork-finger) affecting integration, recovery, and processing complexity. The careful selection of materials—especially substrates, electrodes, and sensing materials—is crucial for sensor efficacy. Despite significant progress in design and application, challenges remain, particularly in mass production, wireless integration, real-time data processing, and long-term stability. To improve mass production feasibility, optimizing fabrication processes, reducing material costs, and incorporating automated production lines are essential for scalability and defect reduction. For wireless integration, enhancing energy efficiency through low-power communication protocols and addressing signal interference and stability are critical for seamless operation. Real-time data processing requires innovative solutions such as edge computing and machine learning algorithms, ensuring low-latency, high-accuracy data interpretation while preserving the flexibility of sensor arrays. Finally, ensuring long-term stability and environmental adaptability demands new materials and protective coatings to withstand harsh conditions. Ongoing research and development are crucial to overcoming these challenges, ensuring that flexible sensor arrays meet the needs of diverse applications while remaining cost-effective and reliable. Full article
(This article belongs to the Special Issue Structural Analyses and Designs for Flexible/Stretchable Electronics, 3rd Edition)
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20 pages, 7265 KiB  
Review
A Review of Wafer-Level Packaging Technology for SAW and BAW Filters
by Xinyue Liu, Wenjiao Pei, Jin Zhao, Rongbin Xu, Yi Zhong and Daquan Yu
Micromachines 2025, 16(3), 320; https://doi.org/10.3390/mi16030320 - 11 Mar 2025
Viewed by 961
Abstract
This paper presents a comprehensive review of advancements in wafer-level packaging (WLP) technology, with a particular focus on its application in surface acoustic wave (SAW) and bulk acoustic wave (BAW) filters. As wireless communication systems continue to evolve, there is an increasing demand [...] Read more.
This paper presents a comprehensive review of advancements in wafer-level packaging (WLP) technology, with a particular focus on its application in surface acoustic wave (SAW) and bulk acoustic wave (BAW) filters. As wireless communication systems continue to evolve, there is an increasing demand for higher performance and miniaturization, which has made acoustic wave devices—especially SAW and BAW filters—crucial components in the Radio Frequency (RF) front-end systems of mobile devices. This review explores key developments in WLP technology, emphasizing novel materials, innovative structures, and advanced modeling techniques that have enabled the miniaturization and enhanced functionality of these filters. Additionally, the paper discusses the role of WLP in addressing challenges related to size reduction and integration, facilitating the creation of multi-functional devices with low manufacturing costs and high precision. Finally, it highlights the opportunities and future directions of WLP technology in the context of next-generation wireless communication standards. Full article
(This article belongs to the Special Issue Emerging Packaging and Interconnection Technology, Second Edition)
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11 pages, 2813 KiB  
Article
Development of an Easy-to-Fabricate Microdevice for Three-Dimensional Culture and Its Application to Glomerular Endothelial Cell Culture
by Miyu Yamazaki, Yasuko Kobayashi and Kiichi Sato
Micromachines 2025, 16(3), 324; https://doi.org/10.3390/mi16030324 - 11 Mar 2025
Viewed by 430
Abstract
The development of an organ-on-a-chip to reproduce organ functions requires the incorporation of a vascular network within the tissue to transport the necessary nutrients. Tissues thicker than 200 µm cannot survive without a capillary network, necessitating the construction of a vascular network exceeding [...] Read more.
The development of an organ-on-a-chip to reproduce organ functions requires the incorporation of a vascular network within the tissue to transport the necessary nutrients. Tissues thicker than 200 µm cannot survive without a capillary network, necessitating the construction of a vascular network exceeding that thickness. Therefore, we focused on the development of an inexpensive and easy-to-fabricate device for thick three-dimensional(3D)-cultured tissues. This device does not have a conventional pillar array structure, and the nutrient supply to the cells from adjacent media channels is not obstructed. Additionally, this device does not require expensive soft lithography equipment or a high-precision 3D printer to fabricate the mold. Human glomerular endothelial cells and human dermal fibroblasts were co-cultured using this device, and a 3D network of vascular endothelial cells (200 µm thick) was successfully constructed. The results of this study are expected to contribute not only to the study of angiogenesis, but also to the development of 3D tissue models that require the incorporation of capillary networks as well as the development of vascularized organ-on-a-chip and disease models for drug screening. Full article
(This article belongs to the Section B2: Biofabrication and Tissue Engineering)
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20 pages, 18781 KiB  
Article
Demonstration of Pattern Size Effects on Hydrophobic Nanocellulose Coatings with Regular Micron-Sized Island-like Geometrical Domains Created by Femtosecond Laser Micromachining
by Pieter Samyn, Patrick Cosemans and Olivier Malek
Micromachines 2025, 16(3), 289; https://doi.org/10.3390/mi16030289 - 28 Feb 2025
Viewed by 444
Abstract
As inspired by nature, wettability of bio-based material surfaces can be controlled by combining appropriate surface chemistries and topographies mimicking the structure of plant leaves or animals. The need for bio-based nanocellulose coatings with enhanced hydrophobic properties becomes technically relevant for extending their [...] Read more.
As inspired by nature, wettability of bio-based material surfaces can be controlled by combining appropriate surface chemistries and topographies mimicking the structure of plant leaves or animals. The need for bio-based nanocellulose coatings with enhanced hydrophobic properties becomes technically relevant for extending their applications in the technological domain with better protection and lifetime of the coatings. In this work, the water repellence of spray-coated nanocellulose coatings with hydrophobically modified cellulose microfiber (mCMF coatings), or hydrophobically modified cellulose nanofiber (mCNF coatings) was enhanced after femtosecond laser patterning. In particular, the influences of different island-like pattern geometries and pattern sizes were systematically studied. The island-like patterns were experimentally created with single posts that have variable sizes of the valleys (B = 30 to 15 µm) and top surface area (T = 120 to 15 µm), resulting in good resolution of the patterns down to the size of the laser beam diameter (15 µm). Depending on the intrinsic homogeneity and porosity of sprayed mCMF and mCNF coatings, the quality and resolution of the island-like patterns is better for the mCNF coatings with thinner and more homogeneous sizes of the cellulose nanofibrils. The increase in apparent water contact angle on patterned nanocellulose coatings can be estimated from the theoretical Cassie–Baxter state of wetting and shows maximum values up to θs = 128° (mCMF coatings), or θs = 140° (mCNF coatings), for the smallest pattern sizes in parallel with minimum contact angle hysteresis of Δθ = 14° (mCMF coatings), or Δθ < 9° (mCNF coatings). The study demonstrated that femtosecond laser patterning technology provides high flexibility and adaptivity to create surface patterns in appropriate dimensions with enhanced hydrophobicity of nanocellulose coatings. Full article
(This article belongs to the Special Issue Laser Micro/Nano-Fabrication)
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17 pages, 3028 KiB  
Review
Advances in Triboelectric Nanogenerators for Microbial Disinfection
by Jinyoung Jeon, Donghyeon Kang and Sang-Woo Kim
Micromachines 2025, 16(3), 281; https://doi.org/10.3390/mi16030281 - 27 Feb 2025
Viewed by 576
Abstract
The global COVID-19 pandemic has highlighted the pivotal role of microbial disinfection technologies, driving the demand for innovative, efficient, and sustainable solutions. Triboelectric technology, known for efficiently converting ambient mechanical energy into electrical energy, has emerged as a promising candidate to address these [...] Read more.
The global COVID-19 pandemic has highlighted the pivotal role of microbial disinfection technologies, driving the demand for innovative, efficient, and sustainable solutions. Triboelectric technology, known for efficiently converting ambient mechanical energy into electrical energy, has emerged as a promising candidate to address these needs. Self-powered electro-based microbial disinfection using triboelectric nanogenerators (TENGs) has emerged as a promising solution. TENGs have demonstrated effective disinfection capabilities in various settings, including water, air, surfaces, and wounds. This review explores the advancements in TENG-based microbial disinfection, highlighting its mechanisms and applications. By utilizing triboelectric technology, it provides comprehensive insights into the development of sustainable and efficient solutions for microbial control across diverse environments. Full article
(This article belongs to the Special Issue Triboelectric Nanogenerators: From Fundamental Research to Applications)
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55 pages, 13764 KiB  
Review
Progress in Polycrystalline SiC Growth by Low Pressure Chemical Vapor Deposition and Material Characterization
by Michail Gavalas, Yann Gallou, Didier Chaussende, Elisabeth Blanquet, Frédéric Mercier and Konstantinos Zekentes
Micromachines 2025, 16(3), 276; https://doi.org/10.3390/mi16030276 - 27 Feb 2025
Viewed by 731
Abstract
The purpose of this paper is to give a review on the state of the art of polycrystalline SiC material grown by low-pressure chemical vapor deposition (LPCVD). Nowadays, LPCVD is the main technique used for the deposition of polycrystalline SiC, both in academic [...] Read more.
The purpose of this paper is to give a review on the state of the art of polycrystalline SiC material grown by low-pressure chemical vapor deposition (LPCVD). Nowadays, LPCVD is the main technique used for the deposition of polycrystalline SiC, both in academic research and industry. Indeed, the LPCVD technique is today the most mature technique to grow high purity polycrystalline thin films with controlled thickness and structure over a large area (>50 cm) and/or 3D substrate. Its ability to have a high degree of modification on the growth conditions and the chosen precursor system allows the deposition of polycrystalline SiC films in various substrates with tailored properties according to the desired application. After a short introduction on the SiC material and its growth by the LPCVD technique, a review of theoretical studies (thermodynamics and kinetics) related to the CVD SiC growth process is given. A synthesis of the experimental studies is made focusing on the effect of the growth conditions on the properties of the deposited SiC polycrystalline material. Despite the numerous results, a full understanding of them is limited due to the complexity of the LPCVD process and the polycrystalline SiC structure. The conclusions show that the growth conditions, like temperature, chamber pressure, (C/Si)(g), (Cl/Si)(g), and doping have an impact on the microstructure and on the corresponding properties of the polycrystalline SiC films. Future perspectives are given in order to improve our understanding on the polycrystalline–SiC–LPCVD process and to enable the growth of tailor-made polycrystalline SiC films for future applications. Full article
(This article belongs to the Special Issue Research Progress of Advanced SiC Semiconductors)
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15 pages, 11779 KiB  
Article
Electrospun Polycaprolactone (PCL) Nanofibers Induce Elongation and Alignment of Co-Cultured Primary Cortical Astrocytes and Neurons
by Kayleigh Nutt, Zoe Dombros-Ryan, Ruxandra Birea, Emily Victoria Franks, Sarah Eastham, Morgan Godwin, Chris F. Adams, Divya Maitreyi Chari and Stuart Iain Jenkins
Micromachines 2025, 16(3), 256; https://doi.org/10.3390/mi16030256 - 25 Feb 2025
Viewed by 993
Abstract
Neuromimetic in vitro models, simulating in vivo architecture/organization, are urgently needed to reduce experimental reliance on live animals. Our group recently reported a novel brain tissue derivation protocol, simultaneously deriving all major cortical cell types (including immune cells) in a facile protocol, generating [...] Read more.
Neuromimetic in vitro models, simulating in vivo architecture/organization, are urgently needed to reduce experimental reliance on live animals. Our group recently reported a novel brain tissue derivation protocol, simultaneously deriving all major cortical cell types (including immune cells) in a facile protocol, generating a network of neurons in a single growth medium, which was interfaced with nanomaterials. This represents a significant advance, as tissue engineers overwhelmingly use diverse methods to derive and combine individual brain cells for materials-interfacing. However, this multicellular model lacked cellular directionality/structural organization (unlike the highly organized cortical circuits in vivo). Synthetic nanofiber constructs are of high value in tissue engineering, providing directional cues for cells. Most neuro-nanofiber studies employ simple monocultures of astrocytes/neurons and commonly use peripheral neurons rather than central nervous system populations. Here, we have interfaced our complex brain model (neurons/astrocytes derived simultaneously) with randomly oriented or aligned polycaprolactone (PCL) fiber meshes. Both cell types showed targeted extension along aligned fibers versus coverslips or random fibers. A new analysis method developed in-house demonstrated that peak orientations for astrocytes and neurons correlated with aligned nanofibers. Our data support the concept that nanofiber scaffolds can achieve organized growth of mixed cortical neural cell populations, mimicking neural architecture. Full article
(This article belongs to the Special Issue The 15th Anniversary of Micromachines)
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18 pages, 13167 KiB  
Article
Research on Low-Profile Directional Flexible Antenna with 3D Coplanar Waveguide for Partial Discharge Detection
by Yan Mi, Wentao Liu, Yiqin Peng, Lei Deng, Benxiang Shu, Xiaopeng Wang and Songyuan Li
Micromachines 2025, 16(3), 253; https://doi.org/10.3390/mi16030253 - 24 Feb 2025
Viewed by 524
Abstract
Due to the challenges in antenna installation and detection performance caused by metal obstruction along the propagation path at a Gas-Insulated Switchgear (GIS) cable terminal, as well as the adverse effects of environmental interference on the detection of partial discharge (PD) by existing [...] Read more.
Due to the challenges in antenna installation and detection performance caused by metal obstruction along the propagation path at a Gas-Insulated Switchgear (GIS) cable terminal, as well as the adverse effects of environmental interference on the detection of partial discharge (PD) by existing flexible antennas, this paper proposes a directional flexible antenna design to mitigate these issues and improve detection performance. The proposed design employs a coplanar waveguide (CPW)-fed monopole antenna structure, where the grounding plane is extended to the back of the antenna to enhance directional reception. The designed flexible antenna measures 88.5 × 70 × 20 mm, and its low-profile design allows it to be easily mounted on the outer wall of the epoxy sleeve at the GIS cable terminal. The measurement results show that the flexible antenna has a Voltage Standing Wave Ratio (VSWR) of less than 2 in the 0.541–3 GHz frequency range. It also maintains stable impedance characteristics across various bending radii, with an average effective height of 10.79 mm in the 0.3–1.5 GHz frequency range. A GIS cable terminal PD experimental platform was established, and the experimental results demonstrate that the bending has minimal impact on the detection performance of the flexible antenna, which can cover the detection range of the GIS cable terminal; metal obstruction significantly impacts the PD signal amplitude, and the designed flexible antenna is suitable for detecting PDs in confined spaces with metal obstruction. Full article
(This article belongs to the Section E：Engineering and Technology)
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20 pages, 7741 KiB  
Article
A Mode-Localized Micro-Electromechanical System Accelerometer with Force Rebalance Closed-Loop Control
by Bowen Wang, Zhenxiang Qi, Kunfeng Wang, Zhaoyang Zhai, Zheng Wang and Xudong Zou
Micromachines 2025, 16(3), 248; https://doi.org/10.3390/mi16030248 - 21 Feb 2025
Viewed by 2009
Abstract
This article proposes a force rebalance control scheme based on a mode-localized resonant accelerometer (ML-RXL), which is applied to address the limited measurement range problem of the ML-RXL. For the first time, an empirical response model of the weakly coupling resonators for the [...] Read more.
This article proposes a force rebalance control scheme based on a mode-localized resonant accelerometer (ML-RXL), which is applied to address the limited measurement range problem of the ML-RXL. For the first time, an empirical response model of the weakly coupling resonators for the amplitude ratio output is established. Based on this, this paper builds an overall model of the force rebalance control system to analyze the sensitivity characteristics by simulations, which demonstrates that the scheme can effectively broaden the linear measurement range. It is demonstrated that the sensor exhibits a highly linear output within a measurement range of ±1 g, with a sensitivity of the feedback-control voltage output measured at 2.94 V/g. The measurement range is expanded by at least 6.7 times. Moreover, the results show that the minimum input-referred acceleration noise density of the sensor for the force rebalance control scheme is 3.29 μg/rtHz, and that the best bias instability is optimized to 5.34 μg with an integral time of 0.64 s. Full article
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26 pages, 3046 KiB  
Review
Polymerase Chain Reaction Chips for Biomarker Discovery and Validation in Drug Development
by Dang-Khoa Vo and Kieu The Loan Trinh
Micromachines 2025, 16(3), 243; https://doi.org/10.3390/mi16030243 - 20 Feb 2025
Viewed by 868
Abstract
Polymerase chain reaction (PCR) chips are advanced, microfluidic platforms that have revolutionized biomarker discovery and validation because of their high sensitivity, specificity, and throughput levels. These chips miniaturize traditional PCR processes for the speed and precision of nucleic acid biomarker detection relevant to [...] Read more.
Polymerase chain reaction (PCR) chips are advanced, microfluidic platforms that have revolutionized biomarker discovery and validation because of their high sensitivity, specificity, and throughput levels. These chips miniaturize traditional PCR processes for the speed and precision of nucleic acid biomarker detection relevant to advancing drug development. Biomarkers, which are useful in helping to explain disease mechanisms, patient stratification, and therapeutic monitoring, are hard to identify and validate due to the complexity of biological systems and the limitations of traditional techniques. The challenges to which PCR chips respond include high-throughput capabilities coupled with real-time quantitative analysis, enabling researchers to identify novel biomarkers with greater accuracy and reproducibility. More recent design improvements of PCR chips have further expanded their functionality to also include digital and multiplex PCR technologies. Digital PCR chips are ideal for quantifying rare biomarkers, which is essential in oncology and infectious disease research. In contrast, multiplex PCR chips enable simultaneous analysis of multiple targets, therefore simplifying biomarker validation. Furthermore, single-cell PCR chips have made it possible to detect biomarkers at unprecedented resolution, hence revealing heterogeneity within cell populations. PCR chips are transforming drug development, enabling target identification, patient stratification, and therapeutic efficacy assessment. They play a major role in the development of companion diagnostics and, therefore, pave the way for personalized medicine, ensuring that the right patient receives the right treatment. While this tremendously promising technology has exhibited many challenges regarding its scalability, integration with other omics technologies, and conformity with regulatory requirements, many still prevail. Future breakthroughs in chip manufacturing, the integration of artificial intelligence, and multi-omics applications will further expand PCR chip capabilities. PCR chips will not only be important for the acceleration of drug discovery and development but also in raising the bar in improving patient outcomes and, hence, global health care as these technologies continue to mature. Full article
(This article belongs to the Special Issue PCR Chips for Biomarker Discovery and Validation in Drug Development)
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20 pages, 5569 KiB  
Review
Design and Fabrication of Microelectrodes for Dielectrophoresis and Electroosmosis in Microsystems for Bio-Applications
by Mengren Wu, Zijian Liu and Yuan Gao
Micromachines 2025, 16(2), 190; https://doi.org/10.3390/mi16020190 - 7 Feb 2025
Cited by 1 | Viewed by 3412
Abstract
Microfluidic technology has emerged as a multidisciplinary field, integrating fluid dynamics, electronics, materials science, etc., enabling precise manipulation of small volumes of fluids and particles for various bio-applications. Among the forms of energy integrated into microfluidic systems, electric fields are particularly advantageous for [...] Read more.
Microfluidic technology has emerged as a multidisciplinary field, integrating fluid dynamics, electronics, materials science, etc., enabling precise manipulation of small volumes of fluids and particles for various bio-applications. Among the forms of energy integrated into microfluidic systems, electric fields are particularly advantageous for achieving precise control at the microscale. This review focuses on the design and fabrication of microelectrodes that drive electrokinetic phenomena, dielectrophoresis (DEP) and electroosmotic flow (EOF), key techniques for particle and fluid manipulation in microfluidic devices. DEP relies on non-uniform electric fields to manipulate particles based on their dielectric properties, while EOF utilizes uniform electric fields to generate consistent fluid flow across microchannels. Advances in microelectrode fabrication, including photolithography, soft lithography, and emerging non-cleanroom techniques, are discussed. Additionally, the review explores innovative approaches such as rapid prototyping, contactless electrodes, and three-dimensional structures, along with material considerations like conductive polymers and carbon composites. The review discusses the role of microelectrodes in enhancing device functionality, scalability, and reliability. The paper also identifies challenges, including the need for improved fabrication reproducibility and multifunctional integration. Finally, potential future research directions are proposed to further optimize DEP- and EOF-based microsystems for advanced biomedical and diagnostic applications. Full article
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25 pages, 3015 KiB  
Review
Magnetic Polymeric Conduits in Biomedical Applications
by Sayan Ganguly and Shlomo Margel
Micromachines 2025, 16(2), 174; https://doi.org/10.3390/mi16020174 - 31 Jan 2025
Cited by 2 | Viewed by 1111
Abstract
Magnetic polymeric conduits are developing as revolutionary materials in regenerative medicine, providing exceptional benefits in directing tissue healing, improving targeted medication administration, and facilitating remote control via external magnetic fields. The present article offers a thorough examination of current progress in the design, [...] Read more.
Magnetic polymeric conduits are developing as revolutionary materials in regenerative medicine, providing exceptional benefits in directing tissue healing, improving targeted medication administration, and facilitating remote control via external magnetic fields. The present article offers a thorough examination of current progress in the design, construction, and functionalization of these hybrid systems. The integration of magnetic nanoparticles into polymeric matrices confers distinctive features, including regulated alignment, improved cellular motility, and targeted medicinal delivery, while preserving structural integrity. Moreover, the incorporation of multifunctional attributes, such as electrical conductivity for cerebral stimulation and optical characteristics for real-time imaging, expands their range of applications. Essential studies indicate that the dimensions, morphology, surface chemistry, and composition of magnetic nanoparticles significantly affect their biocompatibility, degrading characteristics, and overall efficacy. Notwithstanding considerable advancements, issues concerning long-term biocompatibility, biodegradability, and scalability persist, in addition to the must for uniform regulatory frameworks to facilitate clinical translation. Progress in additive manufacturing and nanotechnology is overcoming these obstacles, facilitating the creation of dynamic and adaptive conduit structures designed for particular biomedical requirements. Magnetic polymeric conduits, by integrating usefulness and safety, are set to transform regenerative therapies, presenting a new avenue for customized medicine and advanced healthcare solutions. Full article
(This article belongs to the Special Issue Technological Advances in Polymer Microfabrication: Design and Processing Innovations, 2nd Edition)
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23 pages, 5559 KiB  
Review
Propulsion Mechanisms in Magnetic Microrobotics: From Single Microrobots to Swarms
by Lanlan Jia, Guangfei Su, Mengyu Zhang, Qi Wen, Lihong Wang and Junyang Li
Micromachines 2025, 16(2), 181; https://doi.org/10.3390/mi16020181 - 31 Jan 2025
Viewed by 1410
Abstract
Microrobots with different structures can exhibit multiple propulsion mechanisms under external magnetic fields. Swarms dynamically assembled by microrobots inherit the advantages of single microrobots, such as degradability and small dimensions, while also offering benefits like scalability and high flexibility. With control of magnetic [...] Read more.
Microrobots with different structures can exhibit multiple propulsion mechanisms under external magnetic fields. Swarms dynamically assembled by microrobots inherit the advantages of single microrobots, such as degradability and small dimensions, while also offering benefits like scalability and high flexibility. With control of magnetic fields, these swarms demonstrate diverse propulsion mechanisms and can perform precise actions in complex environments. Therefore, the relationship between single microrobots and their swarms is a significant area of study. This paper reviews the relationship between single microrobots and swarms by examining the structural design, control methods, propulsion mechanisms, and practical applications. At first, we introduce the structural design of microrobots, including materials and manufacturing methods. Then, we describe magnetic field generation systems, including gradient, rotating, and oscillating magnetic fields, and their characteristics. Next, we analyze the propulsion mechanisms of individual microrobots and the way microrobots dynamically assemble into a swarm under an external magnetic field, which illustrates the relationship between single microrobots and swarms. Finally, we discuss the application of different swarm propulsion mechanisms in water purification and targeted delivery, summarize current challenges and future work, and explore future directions. Full article
(This article belongs to the Special Issue Magnetic Microrobots for Biomedical Applications, Second Edition)
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16 pages, 8989 KiB  
Article
Microneedle-Assisted Delivery of Curcumin: Evaluating the Effects of Needle Length and Formulation
by Em-on Chaiprateep, Soma Sengupta and Cornelia M. Keck
Micromachines 2025, 16(2), 155; https://doi.org/10.3390/mi16020155 - 29 Jan 2025
Viewed by 1008
Abstract
Dermal drug delivery presents a significant challenge for poorly soluble active compounds like curcumin, which often struggle to penetrate the skin barrier effectively. In this study, the dermal penetration efficacy of curcumin nanocrystals and bulk suspensions when applied to skin using microneedles of [...] Read more.
Dermal drug delivery presents a significant challenge for poorly soluble active compounds like curcumin, which often struggle to penetrate the skin barrier effectively. In this study, the dermal penetration efficacy of curcumin nanocrystals and bulk suspensions when applied to skin using microneedles of varying lengths—0.25 mm, 0.5 mm, and 1.0 mm—was investigated in an ex vivo porcine ear model. The findings revealed that all formulations, in conjunction with microneedle application, facilitated transepidermal penetration; however, the combination of microneedles and curcumin nanocrystals demonstrated the highest efficacy. Notably, the 1.0 mm microneedle length provided optimal penetration, significantly enhancing curcumin delivery compared with bulk suspensions alone. Additionally, even the use of 0.25 mm microneedles resulted in a high level of efficiency, indicating that shorter microneedles can still effectively facilitate drug delivery. Overall, this study underscores the potential of microneedle technology in improving the transepidermal absorption of poorly soluble actives like curcumin, suggesting that the integration of nanocrystals with microneedles could enhance the therapeutic effects of topical curcumin applications. Full article
(This article belongs to the Special Issue Current Trends in Microneedles: Design, Fabrication and Applications)
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12 pages, 3482 KiB  
Article
Driving Rotational Circulation in a Microfluidic Chamber Using Dual Focused Surface-Acoustic-Wave Beams
by Jin-Chen Hsu and Kai-Li Liao
Micromachines 2025, 16(2), 140; https://doi.org/10.3390/mi16020140 - 25 Jan 2025
Viewed by 914
Abstract
In this paper, enhanced rotational circulation in a circular microfluidic chamber driven by dual focused surface-acoustic-wave (SAW) beams is presented. To characterize the resonant frequency and focusing effect, we simulate the focused SAW field excited by an arc-shaped interdigital transducer patterned on a [...] Read more.
In this paper, enhanced rotational circulation in a circular microfluidic chamber driven by dual focused surface-acoustic-wave (SAW) beams is presented. To characterize the resonant frequency and focusing effect, we simulate the focused SAW field excited by an arc-shaped interdigital transducer patterned on a 128°Y-cut lithium-niobate (LiNbO3) substrate using a finite element method. A full three-dimensional perturbation model of the combined system of the microfluidic chamber and the SAW device is conducted to obtain the acoustic pressure and acoustic streaming fields, which show rotational acoustic pressure and encircling streaming resulted in the chamber. Accordingly, the SAW acoustofluidic system is realized using microfabrication techniques and applied to perform acoustophoresis experiments on submicron particles suspending in the microfluidic chamber. The result verifies the rotational circulation motion of the streaming flow, which is attributed to enhanced angular momentum flux injection and Eckart streaming effect through the dual focused SAW beams. Our results should be of importance in driving particle circulation and enhancing mass transfer in chamber embedded microfluidic channels, which may have promising applications in accelerating bioparticle or cell reactions and fusion, enhancing biochemical and electrochemical sensing, and efficient microfluidic mixing. Full article
(This article belongs to the Special Issue Surface and Bulk Acoustic Wave Devices)
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23 pages, 6410 KiB  
Article
Automatic Extraction and Compensation of P-Bit Device Variations in Large Array Utilizing Boltzmann Machine Training
by Bolin Zhang, Yu Liu, Tianqi Gao, Jialiang Yin, Zhenyu Guan, Deming Zhang and Lang Zeng
Micromachines 2025, 16(2), 133; https://doi.org/10.3390/mi16020133 - 24 Jan 2025
Viewed by 1175
Abstract
A Probabilistic Bit (P-Bit) device serves as the core hardware for implementing Ising computation. However, the severe intrinsic variations of stochastic P-Bit devices hinder the large-scale expansion of the P-Bit array, significantly limiting the practical usage of Ising computation. In this work, a [...] Read more.
A Probabilistic Bit (P-Bit) device serves as the core hardware for implementing Ising computation. However, the severe intrinsic variations of stochastic P-Bit devices hinder the large-scale expansion of the P-Bit array, significantly limiting the practical usage of Ising computation. In this work, a behavioral model which attributes P-Bit variations to two parameters, α and ΔV, is proposed. Then the weight compensation method is introduced, which can mitigate α and ΔV of P-Bit device variations by rederiving the weight matrix, enabling them to compute as ideal identical P-Bits without the need for weights retraining. Accurately extracting the α and ΔV simultaneously from a large P-Bit array which is prerequisite for the weight compensation method is a crucial and challenging task. To solve this obstacle, we present the novel automatic variation extraction algorithm which can extract device variations of each P-Bit in a large array based on Boltzmann machine learning. In order for the accurate extraction of variations from an extendable P-Bit array, an Ising Hamiltonian based on a 3D ferromagnetic model is constructed, achieving precise and scalable array variation extraction. The proposed Automatic Extraction and Compensation algorithm is utilized to solve both 16-city traveling salesman problem (TSP) and 21-bit integer factorization on a large P-Bit array with variation, demonstrating its accuracy, transferability, and scalability. Full article
(This article belongs to the Special Issue Magnetic and Spin Devices, 3rd Edition)
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48 pages, 12131 KiB  
Review
Review of Layered Transition Metal Oxide Materials for Cathodes in Sodium-Ion Batteries
by Mehdi Ahangari, Meng Zhou and Hongmei Luo
Micromachines 2025, 16(2), 137; https://doi.org/10.3390/mi16020137 - 24 Jan 2025
Viewed by 2069
Abstract
The growing interest in sodium-ion batteries (SIBs) is driven by scarcity and the rising costs of lithium, coupled with the urgent need for scalable and sustainable energy storage solutions. Among various cathode materials, layered transition metal oxides have emerged as promising candidates due [...] Read more.
The growing interest in sodium-ion batteries (SIBs) is driven by scarcity and the rising costs of lithium, coupled with the urgent need for scalable and sustainable energy storage solutions. Among various cathode materials, layered transition metal oxides have emerged as promising candidates due to their structural similarity to lithium-ion battery (LIB) counterparts and their potential to deliver high energy density at reduced costs. However, significant challenges remain, including limited capacity at high charge/discharge rates and structural instability during extended cycling. Addressing these issues is critical for advancing SIB technology toward industrial applications, particularly for large-scale energy storage systems. This review provides a comprehensive analysis of layered sodium transition metal oxides, focusing on their structural properties, electrochemical performance, and degradation mechanisms. Special attention is given to the intrinsic and extrinsic factors contributing to their instability, such as structural phase transitions, and cationic/anionic redox behavior. Additionally, recent advancements in material design strategies, including doping, surface modifications, and composite formation, are discussed to highlight the progress toward enhancing the stability and performance of these materials. This work aims to bridge the knowledge gaps and inspire further innovations in the development of high-performance cathodes for sodium-ion batteries. Full article
(This article belongs to the Special Issue Energy Conversion Materials/Devices and Their Applications)
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16 pages, 5458 KiB  
Article
Enhanced Performance of Novel Amorphous Silicon Carbide Microelectrode Arrays in Rat Motor Cortex
by Pegah Haghighi, Eleanor N. Jeakle, Brandon S. Sturgill, Justin R. Abbott, Elysandra Solis, Veda S. Devata, Gayathri Vijayakumar, Ana G. Hernandez-Reynoso, Stuart F. Cogan and Joseph J. Pancrazio
Micromachines 2025, 16(2), 113; https://doi.org/10.3390/mi16020113 - 21 Jan 2025
Viewed by 1407
Abstract
Implantable microelectrode arrays (MEAs) enable the recording of electrical activity from cortical neurons for applications that include brain–machine interfaces. However, MEAs show reduced recording capabilities under chronic implantation conditions. This has largely been attributed to the brain’s foreign body response, which is marked [...] Read more.
Implantable microelectrode arrays (MEAs) enable the recording of electrical activity from cortical neurons for applications that include brain–machine interfaces. However, MEAs show reduced recording capabilities under chronic implantation conditions. This has largely been attributed to the brain’s foreign body response, which is marked by neuroinflammation and gliosis in the immediate vicinity of the MEA implantation site. This has prompted the development of novel MEAs with either coatings or architectures that aim to reduce the tissue response. The present study examines the comparative performance of multi-shank planar, silicon-based devices and low-flexural-rigidity amorphous silicon carbide (a-SiC) MEAs that have a similar architecture but differ with respect to the shank cross-sectional area. Data from a-SiC arrays were previously reported in a prior study from our group. In a manner consistent with the prior work, larger cross-sectional area silicon-based arrays were implanted in the motor cortex of female Sprague-Dawley rats and weekly recordings were made for 16 weeks after implantation. Single unit metrics from the recordings were compared over the implantation period between the device types. Overall, the expression of single units measured from a-SiC devices was significantly higher than for silicon-based MEAs throughout the implantation period. Immunohistochemical analysis demonstrated reduced neuroinflammation and gliosis around the a-SiC MEAs compared to silicon-based devices. Our findings demonstrate that the a-SiC MEAs with a smaller shank cross-sectional area can record single unit activity with more stability and exhibit a reduced inflammatory response compared to the silicon-based device employed in this study. Full article
(This article belongs to the Special Issue Flexible and Wearable Sensors, 3rd Edition)
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10 pages, 8031 KiB  
Article
An All-in-One Testing Chip for the Simultaneous Measurement of Multiple Thermoelectric Parameters in Doped Polysilicon
by Lei Shi, Na Zhou, Jintao Wu, Meng Shi, Yizhi Shi, Cheng Lei and Haiyang Mao
Micromachines 2025, 16(2), 116; https://doi.org/10.3390/mi16020116 - 21 Jan 2025
Viewed by 754
Abstract
Polysilicon is widely used as a thermoelectric material due to its CMOS compatibility and tunability through doping. The accurate measurement of the thermoelectric parameters—such as the Seebeck coefficient, thermal conductivity, and electrical resistivity—of polysilicon with various doping conditions is essential for designing and [...] Read more.
Polysilicon is widely used as a thermoelectric material due to its CMOS compatibility and tunability through doping. The accurate measurement of the thermoelectric parameters—such as the Seebeck coefficient, thermal conductivity, and electrical resistivity—of polysilicon with various doping conditions is essential for designing and fabricating high-performance thermopile sensors. This work presents an all-in-one testing chip that incorporates double-layer thermoelectric structures on a suspended membrane-based supporting layer, with polysilicon constituting at least one of these thermoelectric layers. By employing a differential calculation approach in conjunction with thermal imaging methods, we could simultaneously measure various thermoelectric parameters—including resistivity, the Seebeck coefficient, and thermal conductivity—of polysilicon under different doping conditions. Furthermore, the method proposed in this study provides a means for accurately obtaining thermoelectric parameters for other materials, thereby facilitating the design and optimization of thermoelectric devices. Full article
(This article belongs to the Special Issue MEMS Sensors and Actuators: Design, Fabrication and Applications)
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13 pages, 2458 KiB  
Article
Temperature-Responsive Hybrid Composite with Zero Temperature Coefficient of Resistance for Wearable Thermotherapy Pads
by Ji-Yoon Ahn, Dong-Kwan Lee, Min-Gi Kim, Won-Jin Kim and Sung-Hoon Park
Micromachines 2025, 16(1), 108; https://doi.org/10.3390/mi16010108 - 19 Jan 2025
Viewed by 880
Abstract
Carbon-based polymer composites are widely used in wearable devices due to their exceptional electrical conductivity and flexibility. However, their temperature-dependent resistance variations pose significant challenges to device safety and performance. A negative temperature coefficient (NTC) can lead to overcurrent risks, while a positive [...] Read more.
Carbon-based polymer composites are widely used in wearable devices due to their exceptional electrical conductivity and flexibility. However, their temperature-dependent resistance variations pose significant challenges to device safety and performance. A negative temperature coefficient (NTC) can lead to overcurrent risks, while a positive temperature coefficient (PTC) compromises accuracy. In this study, we present a novel hybrid composite combining carbon nanotubes (CNTs) with NTC properties and carbon black (CB) with PTC properties to achieve a near-zero temperature coefficient of resistance (TCR) at an optimal ratio. This innovation enhances the safety and reliability of carbon-based polymer composites for wearable heating applications. Furthermore, a thermochromic pigment layer is integrated into the hybrid composite, enabling visual temperature indication across three distinct zones. This bilayer structure not only addresses the TCR challenge but also provides real-time, user-friendly temperature monitoring. The resulting composite demonstrates consistent performance and high precision under diverse heating conditions, making it ideal for wearable thermotherapy pads. This study highlights a significant advancement in developing multifunctional, temperature-responsive materials, offering a promising solution for safer and more controllable wearable devices. Full article
(This article belongs to the Special Issue Feature Papers of Micromachines in 'Materials and Processing' 2024)
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17 pages, 12970 KiB  
Article
Design of Dielectric Elastomer Actuator and Its Application in Flexible Gripper
by Xiaoyu Meng, Jiaqing Xie, Haoran Pang, Wenchao Wei, Jiping Niu, Mingqiang Zhu, Fang Gu, Xiaohuan Fan and Haiyan Fan
Micromachines 2025, 16(1), 107; https://doi.org/10.3390/mi16010107 - 19 Jan 2025
Viewed by 1156
Abstract
Dielectric elastomer actuators (DEAs) are difficult to apply to flexible grippers due to their small deformation range and low output force. Hence, a DEA with a large bending deformation range and output force was designed, and a corresponding flexible gripper was developed to [...] Read more.
Dielectric elastomer actuators (DEAs) are difficult to apply to flexible grippers due to their small deformation range and low output force. Hence, a DEA with a large bending deformation range and output force was designed, and a corresponding flexible gripper was developed to realize the function of grasping objects of different shapes. The relationship between the pre-stretch ratio and DEA deformation degree was tested by experiments. Based on the performance test results of the dielectric elastomer (DE), the bending deformation process of DEAs with different shapes was simulated by Finite Element Method (FEM) simulation. DEAs with different shapes were prepared through laser cutting and the relationship between the voltage and the bending angle, and the output force of the DEAs was measured. The result shows that under uniaxial stretching, the deformation of the DEA in the stretching direction gradually increases and decreases in the unstretched direction with the increase in the pre-stretch ratio. Under biaxial stretching, DEA deformation increases with the increase in the pre-stretch ratio. The shape of the DEA has a certain influence on the bending deformation range under the same conditions, and the elliptical DEA has a larger bending deformation range and higher output force compared with the rectangular DEA and the trapezium DEA. The elliptical DEA can produce a bending deformation of 40° and an output force of 37.2 mN at a voltage of 24 kV. The three-finger flexible gripper composed of an elliptical DEA can realize the grasping of a paper cup. Full article
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11 pages, 3416 KiB  
Article
Efficient Particle Manipulation Using Contraction–Expansion Microchannels Embedded with Hook-Shaped Arrays
by Di Huang, Yan Zhao, Chao Cao and Jiyun Zhao
Micromachines 2025, 16(1), 83; https://doi.org/10.3390/mi16010083 - 13 Jan 2025
Cited by 1 | Viewed by 758
Abstract
Inertial microfluidics, as an efficient method for the manipulation of micro-/nanoparticles, has garnered significant attention due to its advantages of high throughput, structural simplicity, no need for external fields, and sheathless operation. Common structures include straight channels, contraction–expansion array (CEA) channels, spiral channels, [...] Read more.
Inertial microfluidics, as an efficient method for the manipulation of micro-/nanoparticles, has garnered significant attention due to its advantages of high throughput, structural simplicity, no need for external fields, and sheathless operation. Common structures include straight channels, contraction–expansion array (CEA) channels, spiral channels, and serpentine channels. In this study, we developed a CEA channel embedded with hook-shaped microstructures to modify the characteristics of vortices. Through experimental studies, we investigated the particles’ migration mechanisms within the proposed structure. The findings indicated that, in comparison to conventional rectangular microstructures, the particles within the hook-shaped microstructured CEA channels experienced a more pronounced influence from inertial lift forces. Moreover, the magnitude of the second flow within the novel configuration was directly proportional to the channel width, the length of the expansion segment, and the embedding depth of the microstructure. The innovative structure was subsequently employed for particle trapping, focusing, and separation. The experimental outcomes revealed focusing efficiency of up to 99.1% and sorting efficiency of up to 97%. This research holds the potential to enhance the foundational theory of Dean flows and broaden the application spectrum of inertial contraction–expansion microfluidic chips. Full article
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10 pages, 3639 KiB  
Article
On-Chip DNA Assembly via Dielectrophoresis
by Xichuan Rui, Lin-Sheng Wu and Xin Zhao
Micromachines 2025, 16(1), 76; https://doi.org/10.3390/mi16010076 - 11 Jan 2025
Viewed by 807
Abstract
On-chip gene synthesis has the potential to improve the synthesis throughput and reduce the cost exponentially. While there exist several microarray-based oligo synthesis technologies, on-chip gene assembly has yet to be demonstrated. This work introduces a novel on-chip DNA assembly method via dielectrophoresis [...] Read more.
On-chip gene synthesis has the potential to improve the synthesis throughput and reduce the cost exponentially. While there exist several microarray-based oligo synthesis technologies, on-chip gene assembly has yet to be demonstrated. This work introduces a novel on-chip DNA assembly method via dielectrophoresis (DEP) that can potentially be integrated with microarray-based oligo synthesis on the same chip. Our DEP chip can selectively manipulate oligos and guide their movement without perturbing the surrounding fluid medium, thus aiding in DNA assembly. Helical forked electrode design has been optimized for compatibility with DEP, ensuring efficient control over target oligos. By applying an alternating current signal set at 2 MHz, we successfully achieve the desired directed movement of oligonucleotides. Additionally, chemical treatments combined with photoirradiation enabled the connection of complementary gene sequences and the subsequent release of single-stranded DNA products. Sequencing results validate the effective assembly of DNA fragments, approximately 500 base pairs in length, using our DEP device. Full article
(This article belongs to the Collection Micro/Nanoscale Electrokinetics)
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22 pages, 9323 KiB  
Article
Magnetic Field-Assisted Orientation and Positioning of Magnetite for Flexible and Electrically Conductive Sensors
by David Seixas Esteves, Amanda Melo, Sónia Alves, Nelson Durães, Maria C. Paiva and Elsa W. Sequeiros
Micromachines 2025, 16(1), 68; https://doi.org/10.3390/mi16010068 - 8 Jan 2025
Viewed by 1096
Abstract
Magnetic field-assisted control of magnetite location is a promising strategy for developing flexible, electrically conductive sensors with enhanced performance and adjustable properties. This study investigates the effect of static magnetic fields applied on thermoplastic elastomer (TPE) composites with magnetite and multi-walled carbon nanotubes [...] Read more.
Magnetic field-assisted control of magnetite location is a promising strategy for developing flexible, electrically conductive sensors with enhanced performance and adjustable properties. This study investigates the effect of static magnetic fields applied on thermoplastic elastomer (TPE) composites with magnetite and multi-walled carbon nanotubes (MWCNT). The composites were prepared by compression moulding and the magnetic field was applied on the mould cavity during processing. Composites were prepared with a range of concentrations of magnetite (1, 3, and 6 wt.%) and MWCNT (1 and 3 wt.%). The effect of particle concentration on composite viscosity was investigated. Rheological analysis showed that MWCNTs significantly increased the composite viscosity while magnetite had minimal impact, ensuring stable processing and facilitating particle orientation under a static magnetic field. Particle orientation and electrical conductivity were evaluated for the composites prepared with different particle concentrations under different processing temperatures. Magnetic field application at 190 °C enhanced magnetite/MWCNT interactions, substantially reducing electrical resistivity while preserving thermal stability. The composites showed no degradation at 220 °C and above, demonstrating suitability for high-temperature applications requiring thermal resilience. Furthermore, magnetite’s magnetic response facilitated precise sensor positioning and strong adhesion to polyimide substrates at 220 °C. These findings demonstrate a scalable and adaptable approach for enhancing sensor performance and positioning, with broad potential in flexible electronics. Full article
(This article belongs to the Special Issue Feature Papers of Micromachines in 'Materials and Processing' 2024)
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15 pages, 15321 KiB  
Article
Picosecond Laser Etching of Glass Spiral Microfluidic Channel for Microparticles Dispersion and Sorting
by Rong Chen, Shanshan He, Xiansong He, Jin Xie and Xicong Zhu
Micromachines 2025, 16(1), 66; https://doi.org/10.3390/mi16010066 - 7 Jan 2025
Viewed by 953
Abstract
In microfluidic chips, glass free-form microchannels have obvious advantages in thermochemical stability and biocompatibility compared to polymer-based channels, but they face challenges in processing morphology and quality. Hence, picosecond laser etching with galvanometer scanning is proposed to machine spiral microfluidic channels on a [...] Read more.
In microfluidic chips, glass free-form microchannels have obvious advantages in thermochemical stability and biocompatibility compared to polymer-based channels, but they face challenges in processing morphology and quality. Hence, picosecond laser etching with galvanometer scanning is proposed to machine spiral microfluidic channels on a glass substrate. The objective is to disperse and sort microparticles from a glass microchip that is difficult to cut. First, the micropillar array and the spiral microchannel were designed to disperse and sort the particles in microchips, respectively; then, a scanning path with a scanning interval of 5 μm was designed according to the spot diameter in picosecond laser etching; next, the effects of laser power, scanning speed and accumulation times were experimentally investigated regarding the morphology of spiral microchannels; finally, the microfluidic flowing test with 5 μm and 10 μm microparticles was performed to analyze the dispersing and sorting performance. It was shown that reducing the laser power and accumulation times alongside increasing the scanning speed effectively reduced the channel depth and surface roughness. The channel surface roughness reached about 500 nm or less when the laser power was 9 W, the scanning speed was 1000 mm/s, and the cumulative number was 4. The etched micropillar array, with a width of 89 μm and an interval of 97 μm, was able to disperse the different microparticles into the spiral microchannel. Moreover, the spiral-structured channel, with an aspect ratio of 0.51, significantly influenced the velocity gradient distribution, particle focusing, and stratification. At flow rates of 300–600 μL/min, the microparticles produced stable focusing bands. Through the etched microchip, mixed 5 μm and 10 μm microparticles were sorted by stable laminar flow at flow rates of 400–500 μL/min. These findings contribute to the design and processing of high-performance glass microfluidic chips for dispersion and sorting. Full article
(This article belongs to the Special Issue Integrated Photonics and Optoelectronics, 2nd Edition)
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32 pages, 1239 KiB  
Review
A Concise Review of Recent Advancements in Carbon Nanotubes for Aerospace Applications
by Silvia Zecchi, Giovanni Cristoforo, Erik Piatti, Daniele Torsello, Gianluca Ghigo, Alberto Tagliaferro, Carlo Rosso and Mattia Bartoli
Micromachines 2025, 16(1), 53; https://doi.org/10.3390/mi16010053 - 31 Dec 2024
Cited by 1 | Viewed by 2157
Abstract
Carbon nanotubes (CNTs) have attracted significant attention in the scientific community and in the industrial environment due to their unique structure and remarkable properties, including mechanical strength, thermal stability, electrical conductivity, and chemical inertness. Despite their potential, large-scale applications have been limited by [...] Read more.
Carbon nanotubes (CNTs) have attracted significant attention in the scientific community and in the industrial environment due to their unique structure and remarkable properties, including mechanical strength, thermal stability, electrical conductivity, and chemical inertness. Despite their potential, large-scale applications have been limited by challenges such as high production costs and catalyst contamination. In aerospace applications, CNTs have demonstrated considerable promise either in the form of thin layers or as reinforcements in polymer and metal matrices, where they enhance mechanical, thermal, and electromagnetic performance in lightweight composites. In this short review, we provide an overview of CNTs’ properties and structures, explore CNT growth methods, with a focus on chemical vapor deposition (CVD), and examine their integration into aerospace materials both as films and as multifunctional reinforcements. Full article
(This article belongs to the Special Issue MEMS Nano/Micro Fabrication, 2nd Edition)
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24 pages, 9570 KiB  
Article
Fringe Texture Driven Droplet Measurement End-to-End Network Based on Physics Aberrations Restoration of Coherence Scanning Interferometry
by Zhou Zhang, Jiankui Chen, Hua Yang and Zhouping Yin
Micromachines 2025, 16(1), 42; https://doi.org/10.3390/mi16010042 - 30 Dec 2024
Viewed by 750
Abstract
Accurate and efficient measurement of deposited droplets’ volume is vital to achieve zero-defect manufacturing in inkjet printed organic light-emitting diode (OLED), but it remains a challenge due to droplets’ featurelessness. In our work, coherence scanning interferometry (CSI) is utilized to measure the volume. [...] Read more.
Accurate and efficient measurement of deposited droplets’ volume is vital to achieve zero-defect manufacturing in inkjet printed organic light-emitting diode (OLED), but it remains a challenge due to droplets’ featurelessness. In our work, coherence scanning interferometry (CSI) is utilized to measure the volume. However, the CSI redundant sampling and image degradation led by the sample’s transparency decrease the efficiency and accuracy. Based on the prior degradation and strong representation for context, a novel method, volume measurement via fringe distribution module (VMFD), is proposed to directly measure the volume by single interferogram without redundant sampling. Firstly, the 3D point spread function (PSF) for CSI imaging is modeling to relate the degradation and image. Secondly, the Zernike to PSF (ZTP) module is proposed to efficiently compute the aberrations to PSF. Then, a physics aberration restoration network (PARN) is designed to remove the degradation via the channel Transformer and U-net architecture. The long term context is learned by PARN and beneficial to restoration. The restored fringes are used to measure the droplet’s volume by constrained regression network (CRN) module. Finally, the performances on public datasets and the volume measurement experiments show the promising deblurring, measurement precision and efficiency. Full article
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16 pages, 7509 KiB  
Article
Highly Sensitive Non-Dispersive Infrared Gas Sensor with Innovative Application for Monitoring Carbon Dioxide Emissions from Lithium-Ion Battery Thermal Runaway
by Liang Luo, Jianwei Chen, Aisn Gioronara Hui, Rongzhen Liu, Yao Zhou, Haitong Liang, Ziyuan Wang, Haosu Luo and Fei Fang
Micromachines 2025, 16(1), 36; https://doi.org/10.3390/mi16010036 - 29 Dec 2024
Viewed by 4266
Abstract
The safety of power batteries in the automotive industry is of paramount importance and cannot be emphasized enough. As lithium-ion battery technology continues to evolve, the energy density of these batteries increases, thereby amplifying the potential risks linked to battery failures. This study [...] Read more.
The safety of power batteries in the automotive industry is of paramount importance and cannot be emphasized enough. As lithium-ion battery technology continues to evolve, the energy density of these batteries increases, thereby amplifying the potential risks linked to battery failures. This study explores pivotal safety challenges within the electric vehicle sector, with a particular focus on thermal runaway and gas emissions originating from lithium-ion batteries. We offer a non-dispersive infrared (NDIR) gas sensor designed to efficiently monitor battery emissions. Notably, carbon dioxide (CO2) gas sensors are emphasized for their ability to enhance early-warning systems, facilitating the timely detection of potential issues and, in turn, improving the overall safety standards of electric vehicles. In this study, we introduce a novel CO2 gas sensor based on the advanced pyroelectric single-crystal lead niobium magnesium titanate (PMNT), which exhibits exceptionally high pyroelectric properties compared to commercially available materials, such as lithium tantalate single crystals and lead zirconate titanate ceramics. The specific detection rate of PMNT single-crystal pyroelectric infrared detectors is more than four times higher than lithium tantalate single-crystal infrared detectors. The PMNT single-crystal NDIR gas detector is used to monitor thermal runaway in lithium-ion batteries, enabling the rapid and highly accurate detection of gases released by the battery. This research offers an in-depth exploration of real-time monitoring for power battery safety, utilizing the cutting-edge pyroelectric single-crystal gas sensor. Beyond providing valuable insights, the study also presents practical recommendations for mitigating the risks of thermal runaway in lithium-ion batteries, with a particular emphasis on the development of effective warning systems. Full article
(This article belongs to the Special Issue Gas Sensors: From Fundamental Research to Applications)
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13 pages, 5571 KiB  
Article
Tilted-Mode All-Optical Diffractive Deep Neural Networks
by Mingzhu Song, Xuhui Zhuang, Lu Rong and Junsheng Wang
Micromachines 2025, 16(1), 8; https://doi.org/10.3390/mi16010008 - 25 Dec 2024
Viewed by 636
Abstract
Diffractive deep neural networks (D2NNs) typically adopt a densely cascaded arrangement of diffractive masks, leading to multiple reflections of diffracted light between adjacent masks, thereby affecting the network’s inference capability. It is challenging to fully simulate this multiple-reflection phenomenon. To eliminate [...] Read more.
Diffractive deep neural networks (D2NNs) typically adopt a densely cascaded arrangement of diffractive masks, leading to multiple reflections of diffracted light between adjacent masks, thereby affecting the network’s inference capability. It is challenging to fully simulate this multiple-reflection phenomenon. To eliminate this phenomenon, we designed tilted-mode all-optical diffractive deep neural networks (T-D2NNs) and proposed a theoretical model for diffraction propagation in the tilted mode. Simulation results indicate that T-D2NNs address the performance degradation caused by interlayer reflections in D2NNs constructed with high-index diffractive masks. In classification tasks, T-D2NNs achieve better classification results compared to D2NNs that consider interlayer reflections. Full article
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9 pages, 2086 KiB  
Article
White Light-Emitting Flexible Displays with Quantum-Dot Film and Greenish-Blue Organic Light-Emitting Diodes
by Young Woo Kim, Seojin Kim, Chaeyeong Lee, Joo Hyun Jeong, Yun Hyeok Jeong, Yuhwa Bak, Seo Hyeon Kim, Sung Jin Park, Ko Eun Ham, Doeun Lee, Junpyo Song, Youngjin Song, Seung-Chan Jung, Oh Kwan Kwon, Jae-Hee Han, Sang Jik Kwon, Eou-Sik Cho and Yongmin Jeon
Micromachines 2024, 15(12), 1518; https://doi.org/10.3390/mi15121518 - 20 Dec 2024
Viewed by 1092
Abstract
White organic light-emitting diodes (OLEDs) represent a significant technology in the display industry for the achievement of full color. However, sophisticated technologies are required for white light emission. In this paper, we developed a simple white light-emitting display device using a quantum-dot (QD) [...] Read more.
White organic light-emitting diodes (OLEDs) represent a significant technology in the display industry for the achievement of full color. However, sophisticated technologies are required for white light emission. In this paper, we developed a simple white light-emitting display device using a quantum-dot (QD) film and a greenish-blue OLED. The resulting QD-OLED produced a high-purity white color with a color temperature of 6000 K (CIEx,y = 0.32, 0.34) and achieved a maximum brightness of 14,638 cd/m2 at 7 V. This paper reports the fabrication of a white light-emitting QD-OLED with a straightforward structure and technology suitable for flexible displays. Full article
(This article belongs to the Special Issue Organic Electronic-Based Devices for Biomedical Applications)
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12 pages, 3196 KiB  
Article
Hollow Fiber Microreactor Combined with Digital Twin to Optimize the Antimicrobial Evaluation Process
by Kazuhiro Noda, Toshihiro Kasama, Marie Shinohara, Masakaze Hamada, Yukiko T. Matsunaga, Madoka Takai, Yoshikazu Ishii and Ryo Miyake
Micromachines 2024, 15(12), 1517; https://doi.org/10.3390/mi15121517 - 20 Dec 2024
Viewed by 786
Abstract
In order to reproduce pharmacokinetics (PK) profiles seen in vivo, the Hollow Fiber Infection Model (HFIM) is a useful in vitro module in the evaluation of antimicrobial resistance. In order to reduce the consumption of culture medium and drugs, we developed a hollow [...] Read more.
In order to reproduce pharmacokinetics (PK) profiles seen in vivo, the Hollow Fiber Infection Model (HFIM) is a useful in vitro module in the evaluation of antimicrobial resistance. In order to reduce the consumption of culture medium and drugs, we developed a hollow fiber microreactor applicable to the HFIM by integrating the HFIM function. Next, we constructed a novel control method by using the “digital twin” of the microreactor to achieve precise concentration control. By integrating functions of the HFIM, the extra-capillary space volume was reduced to less than 1/10 of conventional HFIM. The control method with the digital twin can keep drug concentration in the extra-capillary space within an error of 10% under simulated drug destruction. The control method with the digital twin can also stabilize the drug concentration both in the intra-capillary space and the extra-capillary space within 15 min. Full article
(This article belongs to the Section C：Chemistry)
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13 pages, 3683 KiB  
Article
Automatic Single-Cell Harvesting for Fetal Nucleated Red Blood Cell Isolation on a Self-Assemble Cell Array (SACA) Chip
by Hsin-Yu Yang, Che-Hsien Lin, Yi-Wen Hu, Chih-Hsuan Chien, Mu-Chi Huang, Chun-Hao Lai, Jen-Kuei Wu and Fan-Gang Tseng
Micromachines 2024, 15(12), 1515; https://doi.org/10.3390/mi15121515 - 20 Dec 2024
Cited by 1 | Viewed by 1224
Abstract
(1) Background: Fetal chromosomal examination is a critical component of modern prenatal testing. Traditionally, maternal serum biomarkers such as free β-human chorionic gonadotropin (Free β-HCG) and pregnancy-associated plasma protein A (PAPPA) have been employed for screening, achieving a detection rate of approximately 90% [...] Read more.
(1) Background: Fetal chromosomal examination is a critical component of modern prenatal testing. Traditionally, maternal serum biomarkers such as free β-human chorionic gonadotropin (Free β-HCG) and pregnancy-associated plasma protein A (PAPPA) have been employed for screening, achieving a detection rate of approximately 90% for fetuses with Down syndrome, albeit with a false positive rate of 5%. While amniocentesis remains the gold standard for the prenatal diagnosis of chromosomal abnormalities, including Down syndrome and Edwards syndrome, its invasive nature carries a significant risk of complications, such as infection, preterm labor, or miscarriage, occurring at a rate of 7 per 1000 procedures. Beyond Down syndrome and Edwards syndrome, other chromosomal abnormalities, such as trisomy of chromosomes 9, 16, or Barr bodies, pose additional diagnostic challenges. Non-invasive prenatal testing (NIPT) has emerged as a powerful alternative for fetal genetic screening by leveraging maternal blood sampling. However, due to the extremely low abundance of fetal cells in maternal circulation, NIPT based on fetal cells faces substantial technical challenges. (2) Methods: Fetal nucleated red blood cells (FnRBCs) were first identified in maternal circulation in a landmark study published in The Lancet in 1959. Due to their fetal origin and presence in maternal peripheral blood, FnRBCs represent an ideal target for non-invasive prenatal testing (NIPT). In this study, we introduce a novel self-assembled cell array (SACA) chip system, a microfluidic-based platform designed to efficiently settle and align cells into a monolayer at the chip’s base within five minutes using lateral flow dynamics and gravity. This system is integrated with a fully automated, multi-channel fluorescence scanning module, enabling the real-time imaging and molecular profiling of fetal cells through fluorescence-tagged antibodies. By employing a combination of Hoechst+/CD71+/HbF+/CD45− markers, the platform achieves the precise enrichment and isolation of FnRBCs at the single-cell level from maternal peripheral blood. (3) Results: The SACA chip system effectively reduces the displacement of non-target cells by 31.2%, achieving a single-cell capture accuracy of 97.85%. This isolation and enrichment system for single cells is well suited for subsequent genetic analysis. Furthermore, the platform achieves a high purity of isolated cells, overcoming the concentration detection limit of short tandem repeat (STR) analysis, demonstrating its capability for reliable non-invasive prenatal testing. (4) Conclusions: This study demonstrates that the SACA chip, combined with an automated image positioning system, can efficiently isolate single fetal nucleated red blood cells (FnRBCs) from 50 million PBMCs in 2 mL of maternal blood, completing STR analysis within 120 min. With higher purification efficiency compared to existing NIPT methods, this platform shows great promise for prenatal diagnostics and potential applications in other clinical fields. Full article
(This article belongs to the Special Issue Application of Microfluidic Technology in Bioengineering)
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9 pages, 2483 KiB  
Article
PET-PZT Dielectric Polarization: Electricity Harvested from Photon Energy
by Alex Nikolov, Sohail Murad and Jongju Lee
Micromachines 2024, 15(12), 1505; https://doi.org/10.3390/mi15121505 - 18 Dec 2024
Cited by 1 | Viewed by 794
Abstract
The effect of residual stress or heat on ferroelectrics used to convert photons into electricity was investigated. The data analysis reveals that when the PET-PZT piezoelectric transducer is UV-irradiated with a 405 nm wavelength, it becomes a photon–heat–stress electric energy converter and capacitator. [...] Read more.
The effect of residual stress or heat on ferroelectrics used to convert photons into electricity was investigated. The data analysis reveals that when the PET-PZT piezoelectric transducer is UV-irradiated with a 405 nm wavelength, it becomes a photon–heat–stress electric energy converter and capacitator. Our objective was to evaluate the PET-PZT photon–heat–stress electric energy conversion performance and the role of the light’s wavelength and intensity. Converting waste energy from energy-intensive processes and systems is crucial to reducing the environmental impact and achieving net-zero emissions. To achieve these, innovative materials are needed to efficiently convert ambient energy into electricity through various physical mechanisms, such as the photovoltaic effect, thermoelectricity, piezoelectricity, triboelectricity, and radiofrequency wireless power transfer. Full article
(This article belongs to the Collection Piezoelectric Transducers: Materials, Devices and Applications)
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21 pages, 4915 KiB  
Review
A Review of Cascaded Metasurfaces for Advanced Integrated Devices
by Lingyun Zhang, Zeyu Zhao, Leying Tao, Yixiao Wang, Chi Zhang, Jianing Yang, Yongqiang Jiang, Huiqi Duan, Xiaoguang Zhao, Shaolong Chen and Zilun Wang
Micromachines 2024, 15(12), 1482; https://doi.org/10.3390/mi15121482 - 10 Dec 2024
Cited by 1 | Viewed by 1825
Abstract
This paper reviews the field of cascaded metasurfaces, which are advanced optical devices formed by stacking or serially arranging multiple metasurface layers. These structures leverage near-field and far-field electromagnetic (EM) coupling mechanisms to enhance functionalities beyond single-layer metasurfaces. This review comprehensively discusses the [...] Read more.
This paper reviews the field of cascaded metasurfaces, which are advanced optical devices formed by stacking or serially arranging multiple metasurface layers. These structures leverage near-field and far-field electromagnetic (EM) coupling mechanisms to enhance functionalities beyond single-layer metasurfaces. This review comprehensively discusses the physical principles, design methodologies, and applications of cascaded metasurfaces, focusing on both static and dynamic configurations. Near-field-coupled structures create new resonant modes through strong EM interactions, allowing for efficient control of light properties like phase, polarization, and wave propagation. Far-field coupling, achieved through greater interlayer spacing, enables traditional optical methods for design, expanding applications to aberration correction, spectrometers, and retroreflectors. Dynamic configurations include tunable devices that adjust their optical characteristics through mechanical motion, making them valuable for applications in beam steering, varifocal lenses, and holography. This paper concludes with insights into the potential of cascaded metasurfaces to create multifunctional, compact optical systems, setting the stage for future innovations in miniaturized and integrated optical devices. Full article
(This article belongs to the Special Issue Terahertz and Infrared Metamaterial Devices, 3nd Edition)
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18 pages, 6309 KiB  
Article
Near-Field Direct Writing Based on Piezoelectric Micromotion for the Programmable Manufacturing of Serpentine Structures
by Xun Chen, Xuanzhi Zhang, Jianfeng Sun, Rongguang Zhang, Xuanyang Liang, Jiecai Long, Jingsong Yao, Xin Chen, Han Wang, Yu Zhang, Jiewu Leng and Renquan Lu
Micromachines 2024, 15(12), 1478; https://doi.org/10.3390/mi15121478 - 7 Dec 2024
Viewed by 1210
Abstract
Serpentine microstructures offer excellent physical properties, making them highly promising in applications in stretchable electronics and tissue engineering. However, existing fabrication methods, such as electrospinning and lithography, face significant challenges in producing microscale serpentine structures that are cost-effective, efficient, and controllable. These methods [...] Read more.
Serpentine microstructures offer excellent physical properties, making them highly promising in applications in stretchable electronics and tissue engineering. However, existing fabrication methods, such as electrospinning and lithography, face significant challenges in producing microscale serpentine structures that are cost-effective, efficient, and controllable. These methods often struggle with achieving precise control over fiber morphology and scalability. In this study, we developed a near-field direct writing (NFDW) technique incorporating piezoelectric micromotion to enable the precise fabrication of serpentine micro-/nanofibers by incorporating micromotion control with macroscopic movement. Modifying the fiber structure allowed for adjustments to the mechanical properties, including tunable extensibility and distinct characteristics. Through the control of the frequency and amplitude of the piezoelectric signal, the printing errors were reduced to below 9.48% in the cycle length direction and 6.33% in the peak height direction. A predictive model for the geometrical extensibility of serpentine structures was derived from Legendre’s incomplete elliptic integral of the second kind and incorporated an error correction factor, which significantly reduced the calculation errors in predicting geometric elongation, by 95.85%. The relationship between microstructure bending and biomimetic non-linear mechanical behavior was explored through tensile testing. By controlling the input electrical signals, highly ordered serpentine microstructures were successfully fabricated, demonstrating potential for use in biomimetic mechanical scaffolds. Full article
(This article belongs to the Special Issue Advanced Manufacturing Technology and Systems, 3rd Edition)
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23 pages, 11501 KiB  
Review
Advances in 2D Molybdenum Disulfide Transistors for Flexible and Wearable Electronics
by Kyoungwon Kwak, Hyewon Yoon, Seongin Hong and Byung Ha Kang
Micromachines 2024, 15(12), 1476; https://doi.org/10.3390/mi15121476 - 5 Dec 2024
Cited by 1 | Viewed by 1519
Abstract
As the trajectory of developing advanced electronics is shifting towards wearable electronics, various methods for implementing flexible and bendable devices capable of conforming to curvilinear surfaces have been widely investigated. In particular, achieving high-performance and stable flexible transistors remains a significant technical challenge, [...] Read more.
As the trajectory of developing advanced electronics is shifting towards wearable electronics, various methods for implementing flexible and bendable devices capable of conforming to curvilinear surfaces have been widely investigated. In particular, achieving high-performance and stable flexible transistors remains a significant technical challenge, as transistors are fundamental components of electronics, playing a key role in overall performance. Among the wide range of candidates for flexible transistors, two-dimensional (2D) molybdenum disulfide (MoS2)-based transistors have emerged as potential solutions to address these challenges. Unlike other 2D materials, the 2D MoS2 offers numerous advantages, such as high carrier mobility, a tunable bandgap, superior mechanical strength, and exceptional chemical stability. This review emphasizes the novel techniques of the fabrication process, structure, and material to achieve flexible MoS2 transistor-based applications. Furthermore, the distinctive feature of this review is its focus on studies published in high-impact journals over the past decade, emphasizing their methods for developing MoS2 transistors into various applications. Finally, the review addresses technical challenges and provides an outlook for flexible and wearable MoS2 transistors. Full article
(This article belongs to the Special Issue New Advances in Wearable and Flexible Sensor Devices and Their Future Prospects)
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59 pages, 20006 KiB  
Review
Magnetoelectric BAW and SAW Devices: A Review
by Bin Luo, Prasanth Velvaluri, Yisi Liu and Nian-Xiang Sun
Micromachines 2024, 15(12), 1471; https://doi.org/10.3390/mi15121471 - 3 Dec 2024
Cited by 2 | Viewed by 2046
Abstract
Magnetoelectric (ME) devices combining piezoelectric and magnetostrictive materials have emerged as powerful tools to miniaturize and enhance sensing and communication technologies. This paper examines recent developments in bulk acoustic wave (BAW) and surface acoustic wave (SAW) ME devices, which demonstrate unique capabilities in [...] Read more.
Magnetoelectric (ME) devices combining piezoelectric and magnetostrictive materials have emerged as powerful tools to miniaturize and enhance sensing and communication technologies. This paper examines recent developments in bulk acoustic wave (BAW) and surface acoustic wave (SAW) ME devices, which demonstrate unique capabilities in ultra-sensitive magnetic sensing, compact antennas, and quantum applications. Leveraging the mechanical resonance of BAW and SAW modes, ME sensors achieve the femto- to pico-Tesla sensitivity ideal for biomedical applications, while ME antennas, operating at acoustic resonance, allow significant size reduction, with high radiation gain and efficiency, which is suited for bandwidth-restricted applications. In addition, ME non-reciprocal magnetoacoustic devices using hybrid magnetoacoustic waves present novel solutions for RF isolation, which have also shown potential for the efficient control of quantum defects, such as negatively charged nitrogen-vacancy (NV−) centers. Continued advancements in materials and device structures are expected to further enhance ME device performance, positioning them as key components in future bio-sensing, wireless communication, and quantum information technologies. Full article
(This article belongs to the Special Issue Novel Surface and Bulk Acoustic Wave Devices)
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29 pages, 10952 KiB  
Review
Resolution Enhancement Strategies in Photoacoustic Microscopy: A Comprehensive Review
by Jinying Zhang, Yifan Shi, Yexiaotong Zhang, Haoran Liu, Shihao Li and Linglu Liu
Micromachines 2024, 15(12), 1463; https://doi.org/10.3390/mi15121463 - 30 Nov 2024
Viewed by 4504
Abstract
Photoacoustic imaging has emerged as a promising modality for medical imaging since its introduction. Photoacoustic microscopy (PAM), which is based on the photoacoustic effect, combines the advantages of both optical and acoustic imaging modalities. PAM facilitates high-sensitivity, high-resolution, non-contact, and non-invasive imaging by [...] Read more.
Photoacoustic imaging has emerged as a promising modality for medical imaging since its introduction. Photoacoustic microscopy (PAM), which is based on the photoacoustic effect, combines the advantages of both optical and acoustic imaging modalities. PAM facilitates high-sensitivity, high-resolution, non-contact, and non-invasive imaging by employing optical absorption as its primary contrast mechanism. The ability of PAM to specifically image parameters such as blood oxygenation and melanin content makes it a valuable addition to the suite of modern biomedical imaging techniques. This review aims to provide a comprehensive overview of the diverse technical approaches and methods employed by researchers to enhance the resolution of photoacoustic microscopy. Firstly, the fundamental principles of the photoacoustic effect and photoacoustic imaging will be presented. Subsequently, resolution enhancement methods for both acoustic-resolution photoacoustic microscopy (AR-PAM) and optical-resolution photoacoustic microscopy (OR-PAM) will be discussed independently. Finally, the aforementioned resolution enhancement methods for photoacoustic microscopy will be critically evaluated, and the current challenges and future prospects of this technology will be summarized. Full article
(This article belongs to the Special Issue Photoacoustic-Based Sensing Systems: Advances, Applications, and Innovative Measurement Strategies)
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14 pages, 4290 KiB  
Article
A Thermal Cycler Based on Magnetic Induction Heating and Anti-Freezing Water Cooling for Rapid PCR
by Yaping Xie, Qin Jiang, Chang Chang, Xin Zhao, Haochen Yong, Xingxing Ke and Zhigang Wu
Micromachines 2024, 15(12), 1462; https://doi.org/10.3390/mi15121462 - 30 Nov 2024
Cited by 2 | Viewed by 1349
Abstract
Distinguished by its exceptional sensitivity and specificity, Polymerase Chain Reaction (PCR) is a pivotal technology for pathogen detection. However, traditional PCR instruments that employ thermoelectric cooling (TEC) are often constrained by cost, efficiency, and performance variability resulting from the fluctuations in ambient temperature. [...] Read more.
Distinguished by its exceptional sensitivity and specificity, Polymerase Chain Reaction (PCR) is a pivotal technology for pathogen detection. However, traditional PCR instruments that employ thermoelectric cooling (TEC) are often constrained by cost, efficiency, and performance variability resulting from the fluctuations in ambient temperature. Here, we present a thermal cycler that utilizes electromagnetic induction heating at 50 kHz and anti-freezing water cooling with a velocity of 0.06 m/s to facilitate rapid heating and cooling of the PCR reaction chamber, significantly enhancing heat transfer efficiency. A multi-physics theoretical heat transfer model, developed using the digital twin approach, enables precise temperature control through advanced algorithms. Experimental results reveal average heating and cooling rates of 14.92 °C/s and 13.39 °C/s, respectively, significantly exceeding those of conventional methods. Compared to commercial PCR instruments, the proposed system further optimizes cost, efficiency, and practicality. Finally, PCR experiments were successfully performed using cDNA (Hepatitis B virus) at various concentrations. Full article
(This article belongs to the Special Issue Feature Papers of Micromachines in Biology and Biomedicine 2024)
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13 pages, 8436 KiB  
Article
Series-Fed Microstrip Patch Antenna Array with Additive-Manufactured Foldable Honeycomb-Shaped Substrate
by Sima Noghanian, Yi-Hsiang Chang, Patricio Guerron and Reena Dahle
Micromachines 2024, 15(12), 1449; https://doi.org/10.3390/mi15121449 - 29 Nov 2024
Viewed by 1211
Abstract
This paper presents a novel foldable S-band microstrip patch antenna array operating in the 2.4–2.45 GHz band. The substrate is designed to allow the array to be folded and arranged in tiles, forming a versatile, reconfigurable antenna array. Additive manufacturing is used to [...] Read more.
This paper presents a novel foldable S-band microstrip patch antenna array operating in the 2.4–2.45 GHz band. The substrate is designed to allow the array to be folded and arranged in tiles, forming a versatile, reconfigurable antenna array. Additive manufacturing is used to fabricate the substrate for ease of fabrication and flexibility in its design. The major challenge in this type of design is creating a proper method of feeding the elements while maintaining the array’s optimal performance. A novel hinge design that can hold a coaxial cable for the series-fed array is introduced. The hinge provides the capability of folding the array from a flat orientation into various folded orientations. In this paper, a 2 × 1 microstrip array unit is presented as proof of concept. The antenna was fabricated and measured, and the results of the measurements are in close agreement with the simulations. The antenna can provide a gain as high as 7.72 dBi in flat conditions. Full article
(This article belongs to the Special Issue Exploring the Potential of 5G and Millimeter-Wave Array Antennas)
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11 pages, 4563 KiB  
Article
A Spectroscopy Solution for Contactless Conductivity Detection in Capillary Electrophoresis
by Tomas Drevinskas, Audrius Maruška, Hirotaka Ihara, Makoto Takafuji, Linas Jonušauskas, Domantas Armonavičius, Mantas Stankevičius, Kristina Bimbiraitė-Survilienė, Elzbieta Skrzydlewska, Ona Ragažinskienė, Yutaka Kuwahara, Shoji Nagaoka, Vilma Kaškonienė and Loreta Kubilienė
Micromachines 2024, 15(12), 1430; https://doi.org/10.3390/mi15121430 - 28 Nov 2024
Viewed by 1139
Abstract
This paper introduces a novel contactless single-chip detector that utilizes impedance-to-digital conversion technology to measure impedance in the microfluidic channel or capillary format analytical device. The detector is designed to operate similarly to capacitively coupled contactless conductivity detectors for capillary electrophoresis or chromatography [...] Read more.
This paper introduces a novel contactless single-chip detector that utilizes impedance-to-digital conversion technology to measure impedance in the microfluidic channel or capillary format analytical device. The detector is designed to operate similarly to capacitively coupled contactless conductivity detectors for capillary electrophoresis or chromatography but with the added capability of performing frequency sweeps up to 200 kHz. At each recorded data point, impedance and phase-shift data can be extracted, which can be used to generate impedance versus frequency plots, or phase-shift versus frequency plots. Real and imaginary parts can also be calculated from the data, allowing for the generation of Nyquist diagrams. This detector represents the first of its kind in the contactless conductivity class to provide spectrum-type data, as demonstrated in capillary electrophoresis experiments. Full article
(This article belongs to the Special Issue Flows in Micro- and Nano-Systems)
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11 pages, 2125 KiB  
Article
Novel Deposition Technique for Fabricating Films with Customized Thickness Profiles
by Chi-Yung Hsieh, Yu-Chi Lin, Xuan-Shan Huang, Jing-Ting Lin and Cheng-Sheng Huang
Micromachines 2024, 15(12), 1412; https://doi.org/10.3390/mi15121412 - 23 Nov 2024
Viewed by 898
Abstract
This study introduces a novel deposition technique capable of depositing thin films with any arbitrary thickness profile. The apparatus consists of a fixed shadow mask and a rotating sample carrier plate. The shadow mask features a specifically designed opening curve that corresponds to [...] Read more.
This study introduces a novel deposition technique capable of depositing thin films with any arbitrary thickness profile. The apparatus consists of a fixed shadow mask and a rotating sample carrier plate. The shadow mask features a specifically designed opening curve that corresponds to the particular thickness profile of the deposited film. We successfully designed two shadow masks and used them to deposit films with linear thickness gradients of 49.3 and 86.8 Å/mm and films with sinusoidal thickness profiles with a period of 40 mm. Furthermore, a linear variable filter was designed on the basis of a quarter-wavelength stack of Si3N4 and SiO2, combined with a TiO2 cavity layer with a linearly varying thickness. By coaxially rotating the sample carrier plate relative to the shadow mask, films with the desired thickness profiles could be fabricated in a single deposition step without the need for additional rotational or translational devices inside the deposition chamber. By rotating the carrier plate, the chips attached at different circumferential positions can achieve consistent thickness profiles, making this method well-suited for mass production. Full article
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15 pages, 5497 KiB  
Article
Spatial Discrimination Limit Analysis of Macrophage Phagocytosis Between Target Antigens and Non-Target Objects Using Microcapillary Manipulation Assay
by Maiha Ando, Dan Horonushi, Haruka Yuki, Shinya Kato, Amane Yoshida and Kenji Yasuda
Micromachines 2024, 15(11), 1394; https://doi.org/10.3390/mi15111394 - 18 Nov 2024
Viewed by 1080
Abstract
During phagocytosis, the FcGR–IgG bond is thought to be necessary to promote cell-membrane extension as the zipper mechanism. However, does this zipper mechanism provide a spatial antigen discrimination capability that allows macrophages to selectively phagocytose only antigens, especially for clusters with a mixture [...] Read more.
During phagocytosis, the FcGR–IgG bond is thought to be necessary to promote cell-membrane extension as the zipper mechanism. However, does this zipper mechanism provide a spatial antigen discrimination capability that allows macrophages to selectively phagocytose only antigens, especially for clusters with a mixture of antigens and non-antigens? To elucidate the ability and limitation of the zipper mechanism, we fed a coupled 2 μm IgG-coated and 4.5 μm non-coated polystyrene bead mixtures to macrophages and observed their phagocytosis. Macrophage engulfed the mixed clusters, including the 4.5 μm non-coated polystyrene part, indicating that the non-coated particles can be engulfed even without the zipper mechanism as far as coupled to the opsonized particles. In contrast, when the non-opsonized particle part was held by the microcapillary manipulation assay, macrophages pinched off the non-coated polystyrene particle part and internalized the opsonized particle part only. The results suggest that (1) an IgG-coated surface is needed to anchor phagocytosis by cell-membrane protrusion; however, (2) once the antibody-dependent cell phagocytosis is started, phagocytosis can proceed with the uncoated objects as the followers of the internalizing opsonized particles even without the support of the zipper mechanism. They may also indicate the concern of misleading the immune system to target unexpected objects because of their aggregation with target pathogens and the possibility of new medical applications to capture the non-opsonized target objects by the aggregation with small antigens to activate an immune response. Full article
(This article belongs to the Section B：Biology and Biomedicine)
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16 pages, 9416 KiB  
Article
An Image Processing Approach to Quality Control of Drop-on-Demand Electrohydrodynamic (EHD) Printing
by Yahya Tawhari, Charchit Shukla and Juan Ren
Micromachines 2024, 15(11), 1376; https://doi.org/10.3390/mi15111376 - 14 Nov 2024
Viewed by 1019
Abstract
Droplet quality in drop-on-demand (DoD) Electrohydrodynamic (EHD) inkjet printing plays a crucial role in influencing the overall performance and manufacturing quality of the operation. The current approach to droplet printing analysis involves manually outlining/labeling the printed dots on the substrate under a microscope [...] Read more.
Droplet quality in drop-on-demand (DoD) Electrohydrodynamic (EHD) inkjet printing plays a crucial role in influencing the overall performance and manufacturing quality of the operation. The current approach to droplet printing analysis involves manually outlining/labeling the printed dots on the substrate under a microscope and then using microscope software to estimate the dot sizes by assuming the dots have a standard circular shape. Therefore, it is prone to errors. Moreover, the dot spacing information is missing, which is also important for EHD DoD printing processes, such as manufacturing micro-arrays. In order to address these issues, the paper explores the application of feature extraction methods aimed at identifying characteristics of the printed droplets to enhance the detection, evaluation, and delineation of significant structures and edges in printed images. The proposed method involves three main stages: (1) image pre-processing, where edge detection techniques such as Canny filtering are applied for printed dot boundary detection; (2) contour detection, which is used to accurately quantify the dot sizes (such as dot perimeter and area); and (3) centroid detection and distance calculation, where the spacing between neighboring dots is quantified as the Euclidean distance of the dot geometric centers. These stages collectively improve the precision and efficiency of EHD DoD printing analysis in terms of dot size and spacing. Edge and contour detection strategies are implemented to minimize edge discrepancies and accurately delineate droplet perimeters for quality analysis, enhancing measurement precision. The proposed image processing approach was first tested using simulated EHD printed droplet arrays with specified dot sizes and spacing, and the achieved quantification accuracy was over 98% in analyzing dot size and spacing, highlighting the high precision of the proposed approach. This approach was further demonstrated through dot analysis of experimentally EHD-printed droplets, showing its superiority over conventional microscope-based measurements. Full article
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