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Micromachines
Special Issues
Biosensors for Biomedical, Agricultural and Environmental Applications
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Biosensors for Biomedical, Agricultural and Environmental Applications

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B1: Biosensors".

Deadline for manuscript submissions: 31 July 2025 | Viewed by 1146

Special Issue Editor

Dr. Hamdi Ben Halima

E-Mail Website
Guest Editor
Institut UTINAM, Université de Franche-Comté, CNRS, F-25000 Besançon, France
Interests: biosensors; immunosensors; electrochemistry

Special Issue Information

Dear Colleagues,

We are glad to announce a Special Issue of Micromachines focusing on "Biosensors for Biomedical, Agricultural and Environmental Applications".

Biosensors are powerful tools for detecting biomarkers, pollutants, xenobiotics, pesticides and contaminants, and their evolution can significantly improve environmental health monitoring and medical diagnostic processes.

Biosensors can be categorized depending on their sensing element, the relevant support, the transduction mechanism or their intended application. Great scientific effort has been devoted to improving and optimizing all of these aspects, aiming to enhance the selectivity and sensitivity of existing sensors while exploring routes to develop novel devices. Contributions stemming from chemistry, biology, physics, engineering, computation and medicine intertwine and complement one another, propelling research in this multidisciplinary, vibrant and dynamic field.

This Special Issue aims to provide an overview of recent advances in the design and production of novel biosensors, with a special focus on novel sensing elements, immobilization techniques, detection strategies and miniaturization. Particular emphasis is placed on aspects related to the micro- and nano-conception of these devices.

Dr. Hamdi Ben Halima
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • environmental sensors
  • biomedical sensors
  • food sensors
  • quality control
  • nanosystems
  • nanomaterials
  • immobilization technologies
  • aptamer-based biosensors
  • enzyme-based biosensors
  • cell-based biosensors
  • DNA-based biosensors
  • immunosensors
  • Lab on a chip (LOC)
  • miniaturization
  • microstructured sensors
  • nanostructured sensors
  • biomarker detection
  • pollutant detection
  • contaminant detection
  • xenobiotic detection

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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13 pages, 6629 KiB  
Article
A Disposable Dopamine Sensor Based on Oxidized Cellulose Nanofibril-Modified SPCE
by Feriel Boussema, Sondes Bourigua, Zayneb Jebali, Hatem Majdoub, Nicole Jaffrezic-Renault and Hamdi Ben Halima
Micromachines 2025, 16(7), 743; https://doi.org/10.3390/mi16070743 - 25 Jun 2025
Viewed by 161
Abstract
Dopamine is a major catecholamine neurotransmitter that plays an essential role in the functioning of the human central nervous system. Imbalances in dopamine levels are associated with neurological disorders and depression. Thus, measuring the concentration of DA in human body fluids is significantly [...] Read more.
Dopamine is a major catecholamine neurotransmitter that plays an essential role in the functioning of the human central nervous system. Imbalances in dopamine levels are associated with neurological disorders and depression. Thus, measuring the concentration of DA in human body fluids is significantly important. In this work, TEMPO-oxidized cellulose nanofibrils (TOCNFs) extracted from marram grass (Ammophilia arenaria), harvested in the central western part of Tunisia, were utilized to modify disposable screen-printed carbon electrodes (SPCEs) for the sensitive detection of dopamine in biological fluids. Differential pulse voltammetry (DPV) measurements displayed a sensitivity of 7.92 µA/µM and a detection limit of 10 nM. The disposable TOCNF-modified SPCE presents a charge transfer coefficient, α, comparable to that of a TOCNF/graphene/AgNP composite-modified GCE. Moreover, it exhibits good repeatability (RSD = 1.9%), good reproducibility (RSD = 2.3%), and appreciable storage stability (91% of its initial response after 3 weeks). The prepared disposable sensor showed satisfactory recovery of dopamine in human urine samples. Full article
(This article belongs to the Special Issue Biosensors for Biomedical, Agricultural and Environmental Applications)
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16 pages, 4491 KiB  
Article
An Electrochemical Immunosensor for Sensitive Detection of Exosomes Based on Au/MXenes and AuPtPdCu
by Jie Gao, Rong Yang, Xiaorui Zhu, Jiling Shi, Sufei Wang and Aihua Jing
Micromachines 2025, 16(3), 280; https://doi.org/10.3390/mi16030280 - 27 Feb 2025
Viewed by 818
Abstract
Exosomes are important biomarkers for liquid biopsy in early cancer screening which play important roles in many biological processes, including apoptosis, inflammatory response, and tumor metastasis. In this study, an electrochemical aptamer immunosensor based on Au/MXene and AuPtPdCu was constructed for the sensitive [...] Read more.
Exosomes are important biomarkers for liquid biopsy in early cancer screening which play important roles in many biological processes, including apoptosis, inflammatory response, and tumor metastasis. In this study, an electrochemical aptamer immunosensor based on Au/MXene and AuPtPdCu was constructed for the sensitive detection of colorectal cancer-derived exosomes. AuNPs were deposited in situ on the surface of MXenes as a sensing platform due to their large specific area, excellent conductivity, and higher number of active sites for aptamer immobilization. The aptamer CD63 immobilized on Au/MXene can specifically capture target exosomes. Therefore, the AuPtPdCu-Apt nanoprobe further enhanced the sensitivity and accuracy of the immunosensor. A low limit of detection of 19 particles μL−1 was achieved in the linear range of 50 to 5 × 104 particles μL−1 under optimal conditions. The immunosensor developed herein showed satisfactory electrochemical stability and anti-interference ability for the detection of exosomes in real serum samples. Full article
(This article belongs to the Special Issue Biosensors for Biomedical, Agricultural and Environmental Applications)
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