Improving nonlinear optics efficiency is currently one of the hotspots in modern optical research. Moreover, with the maturity of nonlinear optical microscope systems, more and more biology, materials, medicine, and other related disciplines have hig...
Scanning nonlinear dielectric microscopy (SNDM) is a near-field microwave-based scanning probe microscopy method with a wide variety of applications, especially in the fields of dielectrics and semiconductors. This microscopy method has often been co...
Differential polarization nonlinear optical microscopy has the potential to become an indispensable tool for structural investigations of ordered biological assemblies and microcrystalline aggregates. Their microscopic organization can be probed thro...
Keratins are one of the main fluorophores of the skin. Keratinization disorders can lead to alterations in the optical properties of the skin. We set out to investigate a rare form of keratinopathic ichthyosis caused by KRT1 mutation with two differe...
Nonlinear microscopy (NM) enables us to investigate the morphology or monitor the physiological processes of the skin through the use of ultrafast lasers. Fiber (or fiber-coupled) lasers are of great interest because they can easily be combined with...
This study describes the adaptation of non-linear microscopy for single-particle tracking (SPT), a method commonly used in biology with single-photon fluorescence. Imaging moving objects with non-linear microscopy raises difficulties due to the scann...
The structure of magnetic domains is an exciting research object that shows an enormous variety of delightful patterns. Epitaxial garnet is one of the most studied magnetic dielectrics with well-recognized bulk domains, while the magnetic composition...
Here we report on a non-linear spectroscopic method for visualization of cold atmospheric plasma (CAP)-induced changes in tissue for reaching a new quality level of CAP application in medicine via online monitoring of wound or cancer treatment. A com...
Optical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observ...
Nonlinear optical (NLO) imaging has emerged as a promising plant cell imaging technique due to its large optical penetration, inherent 3D spatial resolution, and reduced photodamage; exogenous nanoprobes are usually needed for nonsignal target cell a...
Micro- and nanoscale chemical and structural heterogeneities, whether they are intrinsic material properties like grain boundaries or intentionally encoded via nanoscale fabrication techniques, pose a challenge to current material characterization me...
A range of sophisticated imaging techniques have been developed in recent years that can reveal the surface structure of cultural heritage objects with varying precision. In combination with various spectroscopic methods, they allow the study of the...
This article covers selected properties of organic–inorganic thin films of hybrid perovskites with the summary formulas CH3NH3MI3, where M = Pb, Cd, Ge, Sn, Zn. The paper discusses not only the history, general structure, applications of perovs...
In this paper, the modelling of a cutting process of a cold-rolled steel sheet using a symmetrical cutting tool is presented. The fast-changing nonlinear dynamic cutting process was elaborated by means of the finite element method and the computer sy...
Piezoelectric actuators (PEAs) are extensively used for scanning and positioning in scanning probe microscopy (SPM) due to their high precision, simple construction, and fast response. However, there are significant challenges for instrument designer...
The non-invasive depth-resolved imaging of pictorial layers in paintings by means of linear optical techniques represents a challenge in the field of Cultural Heritage (CH). The presence of opaque and/or highly-scattering materials may obstruct the p...
Three-dimensional culture systems and suitable substrates topographies demonstrated to drive stem cell fate in vitro by mechanical conditioning. For example, the Nichoid 3D scaffold remodels stem cells and shapes nuclei, thus promoting stem cell expa...
Nonlinear optical microscopic imaging techniques have advanced for chemically sensitive imaging of solid and liquid samples but lack advancements for gaseous samples. In this work, wide-field three-color ultrafast coherent anti-Stokes Raman scatterin...
Increasing the imaging rate of atomic force microscopy (AFM) without impairing of the imaging quality is a challenging task, since the increase in the scanning speed leads to a number of artifacts related to the limited mechanical bandwidth of the AF...
Ultrafast lasers operating at 740 nm and 820 nm have attracted widespread attention as two-photon light sources for the detection of biological metabolism. Here, we report on a solid-like quartz-encapsulated femtosecond laser with a repetition rate o...
Lately the nonlinear optical third-harmonic generation (THG) microscopy is starting to emerge as a laboratory standard for label-free studies in biological samples. In this study, the THG signals produced from corn starch granules are investigated. I...
We present a novel approach to second harmonic microscopy combining variable temperature and photon counting. This innovative method aims to generate Second Harmonic Generation (SHG) images by scanning the same area multiple times with short pixel dw...
The ability to respond to injury with tissue repair is a fundamental property of all multicellular organisms. The extracellular matrix (ECM), composed of fibrillar collagens as well as a number of other components is dis-regulated during repair in ma...
Although the use of lasers in medical diagnosis and therapies, as well as in fundamental biomedical research is now almost routine, advanced laser sources and new laser-based methods continue to emerge. Due to the unique ability of ultrashort laser p...
This paper modelled the cutting process of a bundle consisted of ultra-thin cold-rolled steel sheets using a guillotine. The geometry of a cutting tool with given dimensions was assumed. A bundle of sheets being cut was modelled as deformable, the cu...
The evolution of laser technologies and the invention of ultrashort laser pulses have resulted in a sharp jump in laser applications in life sciences. Developmental biology is no exception. The unique ability of ultrashort laser pulses to deposit ene...
Fabry disease (FD) is a multisystemic X-linked lysosomal storage disease that presents with angiokeratomas (AKs). Our objective was to investigate the clinical and morphologic features of AKs and to present two experimental techniques, multispectral...
Stimulated Raman Scattering Microscopy (SRS) is a powerful tool for label-free detailed recognition and investigation of the cellular and subcellular structures of living cells. Determining subcellular protein localization from the cell level of SRS...
The precise knowledge of the point spread function is central for any imaging system characterization. In fluorescence microscopy, point spread function (PSF) determination has become a common and obligatory task for each new experimental device, mai...
Photodynamic therapy (PDT) provides a potential therapeutic approach for killing malignant cell/solid tumors, but currently approved photosensitizers (PSs) are generally excited by visible light, limiting the penetration depth in tissues. It is neces...
In this study, we revealed a peculiar morphological feature of 50B11 nociceptive sensory neurons in in vitro culture related to the forskolin-induced differentiation of these cells growing upside-down on cover glass supports. Multi-photon non-linear...
We demonstrate a multimodal superresolution microscopy technique based on a phase masked excitation beam in combination with spatially filtered detection. The theoretical foundation for calculating the focus from a non-paraxial beam with an arbitrary...
Near-infrared (NIR) marker-based imaging is of growing importance for deep tissue imaging and is based on a considerable reduction of optical losses at large wavelengths. We aim to extend the range of NIR excitation wavelengths particularly to values...
Due to the increase in the average age of humans, Alzheimer’s disease (AD) has become one of the disorders with the highest incidence worldwide. Abnormal amyloid β protein (Aβ) accumulation is believed to be the most common cause of AD. Therefore, di...
The CIPHR group, Institute of Physics, University of Tartu, Estonia, and Optical Sciences Center, Swinburne University of Technology, Australia, jointly organized the interdisciplinary online conference “Holography Meets Advanced Manufacturing&...
An innovative two-photon microscope exploiting a line-shaped illumination has been recently devised and then implemented. Such configuration allows to carry out a real-time detection by means of standard CCD cameras and is able to maintain the same r...
Nonlinear plasmonics requires the use of high-intensity laser sources in the visible and near/mid-infrared spectral ranges to characterise the potential enhancement of the vibrational fingerprint of chemically functionalised nanostructured interfaces...
Alzheimer’s disease (AD) is a multifactorial, irreversible, and incurable neurodegenerative disease. The main pathological feature of AD is the deposition of misfolded β-amyloid protein (Aβ) plaques in the brain. The abnormal accumulation of Aβ plaqu...
Mechanical sensors provide core keys for high-end research in quantitative understanding of fundamental phenomena and practical applications such as the force or pressure sensor, accelerometer and gyroscope. In particular, in situ sensitive and relia...
We describe an advanced DIVER (Deep Imaging Via Emission Recovery) detection system for two-photon fluorescence microscopy that allows imaging in multiple scattering media, including biological tissues, up to a depth of a few mm with micron resolutio...
Polarization sensitive second harmonic generation (pSHG) microscopy is an imaging technique able to provide, in a non-invasive manner, information related to the molecular structure of second harmonic generation (SHG) active structures, many of which...
To induce a Raman-active transition in a material, stimulated Raman scattering (SRS) spectroscopy/microscopy implementations typically rely on two pulsed laser sources. One of their limitations is that not all of the regions of Raman spectra can be i...
Alterations in the dynamical properties of an atomic force microscope microcantilever beam system in tapping mode can appreciably impact its measurement precision. Understanding the influence mechanism of dynamic parameter changes on the system&rsquo...
Stimulated Raman scattering (SRS) microscopy is a high-speed imaging modality based on intrinsic molecular vibrations, producing chemical maps in living systems. Such capability, allowing for direct visualization without the perturbation of biologica...
The Fourier transform infrared microspectroscopy (FTIRm) system of the Australian Synchrotron has a unique optical configuration with a peculiar beam profile consisting of two parallel lines. The beam is tightly focused using a 36× Schwarzschil...
The ability to create complex three-dimensional microstructures has reached an unprecedented level of sophistication in the last 15 years. For the most part, this is the result of a steady development of the additive manufacturing technique named two...
Background/Objectives: Wilms tumors, the most common pediatric renal tumors, are heterogeneous and consist of varying amounts of three components: blastema, epithelium, and stroma. Postoperative chemotherapy is tailored based on risk group classifica...
Background/Objectives: Pediatric gonadal tumors are rare tumors, and germ cell tumors (GCTs) are the most common subgroup. GCTs are heterogeneous tumors and have different subtypes that can be either benign or malignant. Therefore, extensive sampling...
Alterations of the β-adrenergic signalling, structural remodelling, and electrical failure of T-tubules are hallmarks of heart failure (HF). Here, we assess the effect of β-adrenoceptor activation on local Ca2+ release in electrically coupled and unc...
D-mannitol is a common polyol that is used as additive in pharmaceutical and personal care product formulations. We investigated its effect on the microstructure and rheology of novel non-aqueous Carbopol dispersions employing traditional and time-re...
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