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Open AccessArticle

Phase Mask-Based Multimodal Superresolution Microscopy

Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, MD 20899, USA
Author to whom correspondence should be addressed.
Photonics 2017, 4(3), 39;
Received: 2 May 2017 / Revised: 22 June 2017 / Accepted: 30 June 2017 / Published: 6 July 2017
(This article belongs to the Special Issue Superresolution Optical Microscopy)
We demonstrate a multimodal superresolution microscopy technique based on a phase masked excitation beam in combination with spatially filtered detection. The theoretical foundation for calculating the focus from a non-paraxial beam with an arbitrary azimuthally symmetric phase mask is presented for linear and two-photon excitation processes as well as the theoretical resolution limitations. Experimentally this technique is demonstrated using two-photon luminescence from 80 nm gold particle as well as two-photon fluorescence lifetime imaging of fluorescent polystyrene beads. Finally to illustrate the versatility of this technique we acquire two-photon fluorescence lifetime, two-photon luminescence, and second harmonic images of a mixture of fluorescent molecules and 80 nm gold particles with <120 nm resolution ( λ /7). Since this approach exclusively relies on engineering the excitation and collection volumes, it is suitable for a wide range of scanning-based microscopies. View Full-Text
Keywords: microscopy; superresolution; nonlinear microscopy microscopy; superresolution; nonlinear microscopy
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MDPI and ACS Style

Beams, R.; Woodcock, J.W.; Gilman, J.W.; Stranick, S.J. Phase Mask-Based Multimodal Superresolution Microscopy. Photonics 2017, 4, 39.

AMA Style

Beams R, Woodcock JW, Gilman JW, Stranick SJ. Phase Mask-Based Multimodal Superresolution Microscopy. Photonics. 2017; 4(3):39.

Chicago/Turabian Style

Beams, Ryan; Woodcock, Jeremiah W.; Gilman, Jeffrey W.; Stranick, Stephan J. 2017. "Phase Mask-Based Multimodal Superresolution Microscopy" Photonics 4, no. 3: 39.

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