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Communication

Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis

1
Department of Dermatology, Venereology and Dermatooncology, Semmelweis University, 1083 Budapest, Hungary
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Department of Dermatology, Szent Janos Hospital, 1125 Budapest, Hungary
3
1st Department of Pathology and Experimental Cancer Research, Semmelweis University, 1083 Budapest, Hungary
4
Institute of Atomic Physics and Spectroscopy, University of Latvia, LV-1586 Riga, Latvia
*
Author to whom correspondence should be addressed.
Academic Editor: Changho Lee
Sensors 2021, 21(4), 1105; https://doi.org/10.3390/s21041105
Received: 10 December 2020 / Revised: 22 January 2021 / Accepted: 2 February 2021 / Published: 5 February 2021
Keratins are one of the main fluorophores of the skin. Keratinization disorders can lead to alterations in the optical properties of the skin. We set out to investigate a rare form of keratinopathic ichthyosis caused by KRT1 mutation with two different optical imaging methods. We used a newly developed light emitting diode (LED) based device to analyze autofluorescence signal at 405 nm excitation and diffuse reflectance at 526 nm in vivo. Mean autofluorescence intensity of the hyperkeratotic palmar skin was markedly higher in comparison to the healthy control (162.35 vs. 51.14). To further assess the skin status, we examined samples from affected skin areas ex vivo by nonlinear optical microscopy. Two-photon excited fluorescence and second-harmonic generation can visualize epidermal keratin and dermal collagen, respectively. We were able to visualize the structure of the epidermis and other skin changes caused by abnormal keratin formation. Taken together, we were able to show that such imaging modalities are useful for the diagnosis and follow-up of keratinopathic diseases. View Full-Text
Keywords: KRT1; keratin; epidermolytic ichthyosis; nonlinear microscopy; multiphoton microscopy; LED; diffuse reflectance; hyperkeratosis; autofluorescence; histopathology KRT1; keratin; epidermolytic ichthyosis; nonlinear microscopy; multiphoton microscopy; LED; diffuse reflectance; hyperkeratosis; autofluorescence; histopathology
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MDPI and ACS Style

Anker, P.; Fésűs, L.; Kiss, N.; Noll, J.; Becker, K.; Kuroli, E.; Mayer, B.; Bozsányi, S.; Lőrincz, K.; Lihacova, I.; Lihachev, A.; Lange, M.; Wikonkál, N.; Medvecz, M. Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis. Sensors 2021, 21, 1105. https://doi.org/10.3390/s21041105

AMA Style

Anker P, Fésűs L, Kiss N, Noll J, Becker K, Kuroli E, Mayer B, Bozsányi S, Lőrincz K, Lihacova I, Lihachev A, Lange M, Wikonkál N, Medvecz M. Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis. Sensors. 2021; 21(4):1105. https://doi.org/10.3390/s21041105

Chicago/Turabian Style

Anker, Pálma, Luca Fésűs, Norbert Kiss, Judit Noll, Krisztina Becker, Enikő Kuroli, Balázs Mayer, Szabolcs Bozsányi, Kende Lőrincz, Ilze Lihacova, Alexey Lihachev, Marta Lange, Norbert Wikonkál, and Márta Medvecz. 2021. "Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis" Sensors 21, no. 4: 1105. https://doi.org/10.3390/s21041105

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