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Comparison of Different Polarization Sensitive Second Harmonic Generation Imaging Techniques

1
Department of Physics, University of Kurdistan, Sanandaj 66177-15175, Iran
2
ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Barcelona, Spain
3
UOC, Universitat Oberta de Catalunya, 08018 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Methods Protoc. 2019, 2(2), 49; https://doi.org/10.3390/mps2020049
Received: 14 May 2019 / Revised: 3 June 2019 / Accepted: 5 June 2019 / Published: 7 June 2019
(This article belongs to the Special Issue Technical Advances in Light Microscopy)
Polarization sensitive second harmonic generation (pSHG) microscopy is an imaging technique able to provide, in a non-invasive manner, information related to the molecular structure of second harmonic generation (SHG) active structures, many of which are commonly found in biological tissue. The process of acquiring this information by means of pSHG microscopy requires a scan of the sample using different polarizations of the excitation beam. This process can take considerable time in comparison with the dynamics of in vivo processes. Fortunately, single scan polarization sensitive second harmonic generation (SS-pSHG) microscopy has also been reported, and is able to generate the same information at a faster speed compared to pSHG. In this paper, the orientation of second harmonic active supramolecular assemblies in starch granules is obtained on by means of pSHG and SS-pSHG. These results are compared in the forward and backward directions, showing a good agreement in both techniques. This paper shows for the first time, to the best of the authors’ knowledge, data acquired using both techniques over the exact same sample and image plane, so that they can be compared pixel-to-pixel. View Full-Text
Keywords: medical and biological imaging; nonlinear microscopy; polarization; second harmonic generation medical and biological imaging; nonlinear microscopy; polarization; second harmonic generation
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MDPI and ACS Style

Alizadeh, M.; Ghotbi, M.; Loza-Alvarez, P.; Merino, D. Comparison of Different Polarization Sensitive Second Harmonic Generation Imaging Techniques. Methods Protoc. 2019, 2, 49.

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