The monitoring of must fermentation in a bioreactor, in which the main physico-chemical parameters are tightly controlled, can provide useful analytical information transferable to winemaking on a larger scale. In this experiment, we followed the gro...
The application of bacterial cellulose (BNC) could be widely expanded if the production costs were reduced. This study aims to determine factors simultaneously affecting the yield and tensile strength of BNC in a newly designed surface air-flow biore...
Studying potential dietary exposure to antimicrobial drug residues via meat and dairy products is essential to ensure human health and consumer safety. When studying how antimicrobial residues in food impact the development of antimicrobial drug resi...
Bone grafts represent a multibillion-dollar industry, with over a million grafts occurring each year. Common graft types are associated with issues such as donor site morbidity in autologous grafts and immunological response in allogenic grafts. Bone...
Combining biomaterial scaffolds with cells serves as a promising strategy for engineering critical size defects; however, homogenous cellular growth within large scaffolds is challenging. Mechanical stimuli can enhance bone regeneration by modulating...
Large-scale bioprocesses often suffer from spatial heterogeneities, which impact microbial performance and often lead to phenotypic population heterogeneity. To better understand these effects at the single-cell level, this study applied, for the fir...
Exosomes from cancer cells are implicated in cancer progression and metastasis, carrying immunosuppressive factors that limit the antitumor abilities of immune cells. The development of a real-time, 3D cell/scaffold construct flow perfusion system ha...
An improved second-order moment bubble-liquid two-phase turbulent model is developed to predict the hydrodynamic characteristics of the shallow bioreactor using two height-to-diameter ratios of H/D = 1.4 and H/D = 2.9. The two-phase hydrodynamic para...
Radial flow perfusion of cell-seeded hollow cylindrical porous scaffolds may overcome the transport limitations of pure diffusion and direct axial perfusion in the realization of bioengineered substitutes of failing or missing tissues. Little has bee...
Calcific aortic valve disease is the most common valvular heart disease in industrialized countries. Pulsatile pressure, sheer and bending stress promote initiation and progression of aortic valve degeneration. The aim of this work is to establish an...
Phenol degradation by Pseudomonas putida BCRC 14365 was investigated at 30 °C and a pH of 5.0–9.0 in the batch tests. Experimental results for both free and immobilized cells demonstrated that a maximum phenol degradation rate occurred at an initial...
The perfusion culture of primary hepatocytes has been widely adopted to build bioreactors for various applications. As a drug testing platform, a unique vertical-flow bioreactor (VfB) array was found to create the compaction culture of hepatocytes wh...
Tangential flow microfiltration is easily adapted for batch and continuous bioreactor clarification. The permeate can be introduced directly to the subsequent capture step. However, the commercial use of tangential flow filtration (TFF) is limited by...
A microfluidic bioreactor with an easy to fabricate nano-plasmonic surface is demonstrated for studies of biofilms and their precursor materials via Surface Enhanced Raman Spectroscopy (SERS). The system uses a novel design to induce sheath flow conf...
Fermentative processes are considered one of the most important technological developments in the modern transforming industry, due to this, the applied research to reach high performance standards with a crucial focus on system intensification, whic...
Tissue engineering, with the goal of repairing or replacing damaged tissue and organs, has continued to make dramatic science-based advances since its origins in the late 1980’s and early 1990’s. Such advances are always multi-disciplinar...
Hydrolysis is the heart of the lignocellulose-to-bioethanol conversion process. Using enzymes to catalyze the hydrolysis represents a more environmentally friendly pathway compared to other techniques. However, for the process to be economically feas...
Mixing operations in biological processes is of utmost importance due to its effect on scaling-up and heat and mass transfer. This paper presents the characterization of a bench-top bioreactor with different impeller configurations, agitation and oxy...
The development of energy-efficient solutions for large-scale fermenters demands a deep and comprehensive understanding of hydrodynamic and heat and mass transfer processes. Despite a wide variety of research dedicated to measurements of mass transfe...
Solid–liquid stirred tanks are widely used in multiphase processes, including bioreactors for mesenchymal stem cell (MSC) culture, yet simultaneous experimental data for both dispersed and carrier phases remain limited. Here, a refractive index...
Critical size skeletal defects resulting from trauma and pathological disorders still remain a major clinical problem worldwide. Bone engineering aims at generating unlimited amounts of viable tissue substitutes by interfacing osteocompetent cells of...
Development of energy-efficient and high-performance bioreactors requires progress in methods for assessing the key parameters of the biosynthesis process. With a wide variety of approaches and methods for determining the phase contact area in gas–li...
Three-dimensional porous scaffolds are substitutes for traditional bone grafts in bone tissue engineering (BTE) applications to restore and treat bone injuries and defects. The use of computational modelling is gaining momentum to predict the paramet...
Single-use bioreactors (SUBs) are revolutionizing biotechnology and biopharmaceutical manufacturing by offering cost-efficient, flexible, and scalable alternatives to traditional reusable systems. These bioreactors, made from disposable and pre-steri...
Increasing worldwide milk manufacturing and dairy processing resulted in producing more effluents, and thus effective management of wastewater is now the most important issue. This study used a new design of a pilot plant-scale hybrid anaerobic labyr...
Anaerobic wastewater treatment is, in many cases, a justified alternative to typical activated sludge processes, from a technological, economic, and ecological point of view. The optimisation of fermentation reactors is primarily concerned with incre...
With the limitation of autografts, the development of alternative treatments for bone diseases to alleviate autograft-related complications is highly demanded. In this study, a tissue-engineered bone was formed by culturing rat bone marrow cells (RBM...
We proposed a specially designed sequential injection (SI) amperometric system coupling with a bioreactor for in-line glucose monitoring in cell culture. The system is composed of three main parts which are the bioreactor, SI system, and electrochemi...
The bi-enzymatic synthesis of the antiviral drug vidarabine (arabinosyladenine, ara-A), catalyzed by uridine phosphorylase from Clostridium perfringens (CpUP) and a purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP), was re-designed un...
The corneal endothelium is responsible for pumping fluid out of the stroma in order to maintain corneal transparency, which depends in part on the expression and activity of sodium–potassium pumps. In this study, we evaluated how physiologic pr...
Due to the heterogeneous nature of large-scale fermentation processes they cannot be modelled as ideally mixed reactors, and therefore flow models are necessary to accurately represent the processes. Computational fluid dynamics (CFD) is used more an...
This study presents findings regarding the kinetics of ferrous iron oxidation in solution mediated by Acidithiobacillus ferrooxidans bacteria within a continuous-flow bioreactor employing diverse types of immobilizers. The objective is to augment the...
Due to its high content of organics and nutrients, swine wastewater has become one of the main environment pollution sources. Exploring high-efficient technologies for swine wastewater treatment is urgent and becoming a hot topic in the recent years....
Perfused bioreactor systems are considered to be a promising approach for the 3D culturing of stem cells by improving the quality of the tissue-engineered grafts in terms of better cell proliferation and deeper penetration of used scaffold materials....
Treatment of nitrate loads by denitrifying bioreactors in centralized drainage ditches that receive subsurface tile drainage may offer a more effective alternative to end-of-pipe bioreactors. A paired denitrifying bioreactor design, consisting of an...
To reduce the operating costs of conventional membrane bioreactors (MBRs) and improve the stability and quality of the dynamic membrane bioreactor (DMBR) effluent, a homemade inexpensive filter cloth assembly was connected to an up-flow ultra-lightwe...
Biotechnological processes involving the presence of microorganisms are realized by using various types of stirred tanks or laboratory-scale dual-impeller commercial bioreactor. Hydrodynamics and mass transfer rate are crucial parameters describing t...
Electrochemical impedance spectroscopy (EIS) is a noninvasive, reliable, and efficient method to analyze the barrier integrity of in vitro tissue models. This well-established tool is used most widely to quantify the transendothelial/epithelial resis...
Key operating variables to predict the necessary scour air flowrate in full-scale Membrane Bioreactor (MBR) systems are identified, aiming to optimize energy consumption while avoiding the limiting condition (i.e., rapid increasing total resistance)....
Anaerobic membrane bioreactors (AnMBRs) require biogas recycling to stir the mixed liquid and flush particles away from the membrane surfaces for stable operation. With the fixed gas cycling rate, gas diffuser configuration is an important factor tha...
Many functions in host–microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota. To analyze these ef...
Salvia apiana Jepson is an endemic North American species characterized by a rich phytochemical profile including abietane-type diterpenoids, phenolic acids, flavonoids, and thujone-free essential oil (EO). The current study was aimed at increasing E...
Gastrointestinal (GI) mucus plays a pivotal role in the tissue homoeostasis and functionality of the gut. However, due to the shortage of affordable, realistic in vitro GI models with a physiologically relevant mucus layer, studies with deeper insigh...
The water industry is becoming increasingly aware of the risks associated with urban supplies not meeting demands by 2050. Greywater (GW) recycling for non-potable uses (e.g., urinal and toilet flushing) provides an urban water management strategy to...
Wastewater reuse has become an important part of the urban water supply portfolio in water stressed regions. Effective wastewater treatment processes are critical to protect public health during water reuse practices. However, the microbial removal e...
Production and consumption of confectionery products have increased worldwide, thus, effective management of wastewater produced is now an important issue. The confectionery high-load sewage was explored for biogas production in an innovative-design...
This paper assesses the performance of an integrated multistage laboratory-scale plant, for the treatment of poultry slaughterhouse wastewater (PSW). The system was comprised of an eco-flush dosed bio-physico pre-treatment unit for fats, oil, and gre...
Electroporation is a technique applied both in biomedical and biotechnological fields which uses a high-voltage electric current to temporarily destabilize the plasma membrane of living cells, permitting the introduction of small molecules as well as...
In this study, an innovative bioreactor, named BioAxFlow, particularly suitable for tissue engineering applications, is tested. Unlike traditional bioreactors, it does not rely on mechanical components to agitate the culture medium, but on the unique...
Pseudomonas aeruginosa (P. aeruginosa) is a Gram-negative bacterium quite versatile that grows [...]
of 7