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5,303 Results Found

  • Article
  • Open Access
14 Citations
3,491 Views
19 Pages

Effect of Sample Collection (Manual Expression vs. Pumping) and Skimming on the Microbial Profile of Human Milk Using Culture Techniques and Metataxonomic Analysis

  • Maricela Rodríguez-Cruz,
  • Claudio Alba,
  • Marina Aparicio,
  • María Ángeles Checa,
  • Leonides Fernández and
  • Juan Miguel Rodríguez

Human milk microbiota is a unique bacterial community playing a relevant role in infant health, but its composition depends on different factors (woman health, lactation stage, and geographical lactation). However, information is lacking regarding so...

  • Review
  • Open Access
3 Citations
3,117 Views
14 Pages

28 December 2024

This article explores recent advancements in non-destructive spectroscopic techniques for identifying pigments in polychrome cultural relics, emphasizing their significance for sustainability in cultural heritage preservation. Techniques such as infr...

  • Feature Paper
  • Review
  • Open Access
25 Citations
6,288 Views
15 Pages

Neutrons for Cultural Heritage—Techniques, Sensors, and Detection

  • Giulia Festa,
  • Giovanni Romanelli,
  • Roberto Senesi,
  • Laura Arcidiacono,
  • Claudia Scatigno,
  • Stewart F. Parker,
  • M. P. M. Marques and
  • Carla Andreani

16 January 2020

Advances in research in Cultural Heritage see increasing application of a multidisciplinary approach and the combined use of physical and chemical characterization of artefacts that can be used to define their structure and their state of conservatio...

  • Article
  • Open Access
8 Citations
7,683 Views
21 Pages

13 March 2019

The Seediq tribe is one of Taiwan’s Indigenous peoples, and they have very traditional weaving techniques. Women of the Seediq weave clothes and quilts for their families as they believe that only women with good weaving skill can pass through...

  • Review
  • Open Access
78 Citations
7,257 Views
13 Pages

4 October 2019

Various tissue engineering techniques have been created in research spanning two centuries, resulting in new opportunities for growing cells in culture and the creation of 3-D tissue-like constructs. These techniques are classified as scaffold-based...

  • Review
  • Open Access
63 Citations
27,268 Views
33 Pages

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

  • Hasan Mehbub,
  • Ayasha Akter,
  • Mst. Arjina Akter,
  • Mohammad Shamim Hasan Mandal,
  • Md. Ashraful Hoque,
  • Monika Tuleja and
  • Hasan Mehraj

23 November 2022

Ornamentals come in a variety of shapes, sizes, and colors to suit a wide range of climates, landscapes, and gardening needs. Compared to demand, a shortage of plant materials and diversity force the search for solutions for their constant acquisitio...

  • Article
  • Open Access
16 Citations
7,194 Views
17 Pages

31 July 2015

This paper proposes to visualize acoustic comfort along tourist routes. Route-based tourism is crucial to the sustainability of tourism development in historic areas. Applying the concept of route-based tourism to guide tourists rambling along cultur...

  • Article
  • Open Access
13 Citations
6,622 Views
16 Pages

19 June 2023

Digital technology can be used to enable the living preservation and heritage innovation of the intangible cultural heritage of Chinese traditional textile skills for sustainable development. Taking the “Wu Leno” weaving technique in Suzh...

  • Article
  • Open Access
1 Citations
1,472 Views
15 Pages

Effects of Saline Solutions on Paper-Based Cultural Heritage: Non-Invasive Techniques for Studying Flooded Ancient Books

  • Tullia Carla David,
  • Francesca Assunta Pisu,
  • Stefania Porcu,
  • Carlo Maria Carbonaro,
  • Jarmila Kodric and
  • Daniele Chiriu

24 January 2025

Archival materials are increasingly vulnerable to damage from chemical, physical, biological, and environmental factors, including climate change-related extreme weather events such as torrential rains and flash floods. These conditions pose signific...

  • Article
  • Open Access
20 Citations
4,688 Views
19 Pages

14 February 2020

A range of sophisticated imaging techniques have been developed in recent years that can reveal the surface structure of cultural heritage objects with varying precision. In combination with various spectroscopic methods, they allow the study of the...

  • Review
  • Open Access
2 Citations
2,875 Views
22 Pages

Development Trend in Non-Destructive Techniques for Cultural Heritage: From Material Characterization to AI-Driven Diagnosis

  • Mingrui Zhang,
  • Suchi Liu,
  • Haojian Shao,
  • Zonghuan Ba,
  • Jie Liu,
  • Mǎdǎlina Georgiana Albu Kaya,
  • Keyong Tang and
  • Guohe Han

16 September 2025

Cultural heritage (CH) relics are irreplaceable records of human civilization, encompassing diverse historical, technological, and artistic achievements. Extracting their structural and compositional information without affecting their physical integ...

  • Feature Paper
  • Article
  • Open Access
3 Citations
4,958 Views
28 Pages

Cultural Heritage Architecture and Climate Adaptation: A Socio-Environmental Analysis of Sustainable Building Techniques

  • Victoria Sanagustín-Fons,
  • Polina Stavrou,
  • José Antonio Moseñe-Fierro,
  • Francisco Escario Sierra,
  • Guido Castrolla,
  • Cândida Rocha and
  • Ester Bazco Nogueras

8 May 2025

This research investigates how historical architectural practices offer valuable solutions for contemporary climate adaptation challenges. Through systematic documentary analysis, we examine how European builders across centuries developed sophistica...

  • Feature Paper
  • Review
  • Open Access
41 Citations
9,076 Views
24 Pages

2 March 2021

Techniques to provide in vitro tissue culture have undergone significant changes during the last decades, and current applications involve interactions of cells and organoids, three-dimensional cell co-cultures, and organ/body-on-chip tools. Efficien...

  • Communication
  • Open Access
4 Citations
2,353 Views
9 Pages

7 June 2023

Due to globally increasing problems concerning biodeterioration of paints, it is worthwhile to enhance the determination of colony forming units (CFU) as a gold standard method via more rapid and culture-independent techniques. Here, we combined trad...

  • Feature Paper
  • Review
  • Open Access
29 Citations
19,154 Views
34 Pages

Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review

  • Luísa Custódio,
  • Gilbert Charles,
  • Christian Magné,
  • Gregorio Barba-Espín,
  • Abel Piqueras,
  • José A. Hernández,
  • Karim Ben Hamed,
  • Viana Castañeda-Loaiza,
  • Eliana Fernandes and
  • Maria João Rodrigues

27 December 2022

Halophytes are plants able to thrive in environments characterized by severe abiotic conditions, including high salinity and high light intensity, drought/flooding, and temperature fluctuations. Several species have ethnomedicinal uses, and some are...

  • Feature Paper
  • Review
  • Open Access
20 Citations
11,118 Views
53 Pages

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part II: Systems and Applications

  • Brigitte Altmann,
  • Christoph Grün,
  • Cordula Nies and
  • Eric Gottwald

23 December 2020

In this second part of our systematic review on the research area of 3D cell culture in micro-bioreactors we give a detailed description of the published work with regard to the existing micro-bioreactor types and their applications, and highlight im...

  • Systematic Review
  • Open Access
3,892 Views
23 Pages

Bioengineering Human Upper Respiratory Mucosa: A Systematic Review of the State of the Art of Cell Culture Techniques

  • Davaine Joel Ndongo Sonfack,
  • Clémence Tanguay Boivin,
  • Lydia Touzel Deschênes,
  • Thibault Maurand,
  • Célina Maguemoun,
  • François Berthod,
  • François Gros-Louis and
  • Pierre-Olivier Champagne

Background: The upper respiratory mucosa plays a crucial role in both the physical integrity and immunological function of the respiratory tract. However, in certain situations such as infections, trauma, or surgery, it might sustain damage. Tissue e...

  • Review
  • Open Access
41 Citations
7,991 Views
22 Pages

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

  • Tomasz Górnicki,
  • Jakub Lambrinow,
  • Afsaneh Golkar-Narenji,
  • Krzysztof Data,
  • Dominika Domagała,
  • Julia Niebora,
  • Maryam Farzaneh,
  • Paul Mozdziak,
  • Maciej Zabel and
  • Paweł Antosik
  • + 5 authors

16 March 2024

Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditi...

  • Review
  • Open Access
3 Citations
4,361 Views
18 Pages

29 December 2023

Over the last two decades, there has been a concerted effort by researchers to mass propagate Eurycoma longifolia and improve the yield of its very important and sought-after anti-cancer and aphrodisiac bioactive compounds. To achieve this, vari...

  • Article
  • Open Access
7 Citations
2,663 Views
11 Pages

Data-Mining Techniques: A New Approach to Identifying the Links among Hybrid Strains of Pleurotus with Culture Media

  • Fabricio Guevara-Viejó,
  • Juan Diego Valenzuela-Cobos,
  • Purificación Vicente-Galindo and
  • Purificación Galindo-Villardón

19 October 2021

In this study, a data set of mycelial and cultural characteristics of hybrid strains of Pleurotus ostreatus and Pleurotus djamor were analyzed using three data-mining techniques: the K-medoids clustering algorithm, PCA biplot and the association rule...

  • Feature Paper
  • Review
  • Open Access
13 Citations
6,338 Views
24 Pages

15 December 2020

Bioreactors have proven useful for a vast amount of applications. Besides classical large-scale bioreactors and fermenters for prokaryotic and eukaryotic organisms, micro-bioreactors, as specialized bioreactor systems, have become an invaluable tool...

  • Article
  • Open Access
4 Citations
3,307 Views
15 Pages

Candida albicans overgrowth at various mucosal sites is an ongoing and complex clinical concern involving interactions with indigenous microbiota and therapeutic or preventive measures superimposed on the pathogen-microbiome interaction. In this pape...

  • Review
  • Open Access
57 Citations
19,362 Views
30 Pages

23 August 2019

Alkaline phosphatase (ALP), which catalyzes the dephosphorylation process of proteins, nucleic acids, and small molecules, can be found in a variety of tissues (intestine, liver, bone, kidney, and placenta) of almost all living organisms. This enzyme...

  • Review
  • Open Access
7 Citations
5,823 Views
41 Pages

MatriGrid® Based Biological Morphologies: Tools for 3D Cell Culturing

  • Patrick Mai,
  • Jörg Hampl,
  • Martin Baca,
  • Dana Brauer,
  • Sukhdeep Singh,
  • Frank Weise,
  • Justyna Borowiec,
  • André Schmidt,
  • Johanna Merle Küstner and
  • Maren Klett
  • + 7 authors

Recent trends in 3D cell culturing has placed organotypic tissue models at another level. Now, not only is the microenvironment at the cynosure of this research, but rather, microscopic geometrical parameters are also decisive for mimicking a tissue...

  • Review
  • Open Access
73 Citations
9,545 Views
21 Pages

11 November 2021

Multicellular tumor spheroids (MCTs) have been employed in biomedical fields owing to their advantage in designing a three-dimensional (3D) solid tumor model. For controlling multicellular cancer spheroids, mimicking the tumor extracellular matrix (E...

  • Article
  • Open Access
5 Citations
3,377 Views
14 Pages

The purpose of this study was to find a way for modern tourists to enjoy increased well-being while being provided with high-quality information about cultural assets. In order for tourists to enjoy well-being, cultural tourism guides must provide qu...

  • Review
  • Open Access
16 Citations
9,853 Views
20 Pages

The Transformative Role of 3D Culture Models in Triple-Negative Breast Cancer Research

  • Xavier S. Bittman-Soto,
  • Evelyn S. Thomas,
  • Madeline E. Ganshert,
  • Laura L. Mendez-Santacruz and
  • J. Chuck Harrell

13 May 2024

Advancements in cell culturing techniques have allowed the development of three-dimensional (3D) cell culture models sourced directly from patients’ tissues and tumors, faithfully replicating the native tissue environment. These models provide...

  • Article
  • Open Access
16 Citations
5,285 Views
16 Pages

Are Business Graduates’ Employability Skills and Learning/Teaching Techniques Universal? Exploring the Role of Culture: A Comparative Study among Australia, China, Pakistan, and Saudi Arabia

  • Muhammad Awais Bhatti,
  • Suzanie Adina Mat Saat,
  • Megbel M. Aleidan,
  • Ghadah Hassan Mohammed Al Murshidi,
  • Mansour Alyahya and
  • Ariff Syah Juhari

6 March 2022

The purpose of this study is to explore the employability skills required in different countries/cultures and to further explore how cultural differences influence teaching/learning techniques required to equip students with required employability sk...

  • Communication
  • Open Access
12 Citations
4,065 Views
10 Pages

The lipidomic analysis of immortalized human meibomian gland epithelial cells (HMGECs) has been proposed as a preclinical model to study meibomian gland dysfunction. An in vitro study was conducted to evaluate neutral lipid recovery following three h...

  • Review
  • Open Access
2 Citations
928 Views
17 Pages

5 September 2025

Plant gums have long served as essential binding media in polychrome cultural heritage, contributing to pigment adhesion, surface cohesion, and long-term stability. This review evaluates recent advances in analytical technologies, including FTIR, Ram...

  • Article
  • Open Access
44 Citations
8,624 Views
16 Pages

Scaling-Up Techniques for the Nanofabrication of Cell Culture Substrates via Two-Photon Polymerization for Industrial-Scale Expansion of Stem Cells

  • Davide Ricci,
  • Michele M. Nava,
  • Tommaso Zandrini,
  • Giulio Cerullo,
  • Manuela T. Raimondi and
  • Roberto Osellame

13 January 2017

Stem-cell-based therapies require a high number (106–109) of cells, therefore in vitro expansion is needed because of the initially low amount of stem cells obtainable from human tissues. Standard protocols for stem cell expansion are currently based...

  • Review
  • Open Access
326 Citations
23,492 Views
26 Pages

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

  • Francisco Javier Ruiz-Ojeda,
  • Azahara Iris Rupérez,
  • Carolina Gomez-Llorente,
  • Angel Gil and
  • Concepción María Aguilera

Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are c...

  • Review
  • Open Access
1,249 Views
12 Pages

Current Progress on Passiflora caerulea L. In Vitro Culturing

  • Pervin Halkoglu-Hristova,
  • Alexandra Garmidolova,
  • Teodora Yaneva and
  • Vasil Georgiev

1 July 2025

Passiflora caerulea L., commonly known as the blue passionflower, is traditionally grown as an ornamental plant, but has a diverse chemical composition resulting in a wide range of biological activities that determine its pharmacological properties a...

  • Article
  • Open Access
2 Citations
3,785 Views
18 Pages

Bulge-Derived Epithelial Cells Isolated from Human Hair Follicles Using Enzymatic Digestion or Explants Result in Comparable Tissue-Engineered Skin

  • Bettina Cattier,
  • Rina Guignard,
  • Israël Martel,
  • Christian Martel,
  • Carolyne Simard-Bisson,
  • Danielle Larouche,
  • Béatrice Guiraud,
  • Sandrine Bessou-Touya and
  • Lucie Germain

21 February 2025

Hair follicle stem cells, located in the bulge region of the outer root sheath, are multipotent epithelial stem cells capable of differentiating into epidermal, sebaceous gland, and hair shaft cells. Efficient culturing of these cells is crucial for...

  • Article
  • Open Access
3 Citations
8,494 Views
11 Pages

Gram Stain and Culture of Sputum Samples Detect Only Few Pathogens in Community-Acquired Lower Respiratory Tract Infections: Secondary Analysis of a Randomized Controlled Trial

  • Mariana B. Cartuliares,
  • Helene Skjøt-Arkil,
  • Christian B. Mogensen,
  • Thor A. Skovsted,
  • Steen L. Andersen,
  • Andreas K. Pedersen and
  • Flemming S. Rosenvinge

Identification of the bacterial etiology of lower respiratory tract infections (LRTI) is crucial to ensure a narrow-spectrum, targeted antibiotic treatment. However, Gram stain and culture results are often difficult to interpret as they depend stron...

  • Article
  • Open Access
2,222 Views
13 Pages

Enhancing Rumen-Undegradable Protein via Processing Techniques in a Dual-Flow Continuous Culture System

  • K. E. Loregian,
  • M. J. Silva,
  • S. B. Dourado,
  • J. Guimarães,
  • B. R. Amâncio,
  • E. Magnani,
  • T. H. Silva,
  • R. H. Branco,
  • P. Del Bianco Benedeti and
  • E. M. Paula

The use of processing techniques to increase the rumen-undegradable protein (RUP) content of protein meals aims to enhance the nutritional performance of high-performance animals. This study evaluated the effects of various processing techniques appl...

  • Article
  • Open Access
8 Citations
3,020 Views
12 Pages

31 August 2022

Unique Indigenous cultures have become increasingly attractive and prevalent in the tourism market. More and more Indigenous tribes wish to improve their economic situation by developing a tourism industry with ethnic culture as the core attraction....

  • Review
  • Open Access
1 Citations
10,008 Views
52 Pages

Detection and Identification of Food-Borne Yeasts: An Overview of the Relevant Methods and Their Evolution

  • Mónika Kovács,
  • Andrea Pomázi,
  • Andrea Taczman-Brückner,
  • Gabriella Kiskó,
  • Viktória Dobó,
  • Tamás Kocsis,
  • Csilla Mohácsi-Farkas and
  • Ágnes Belák

The presence of yeasts in food is not unexpected, as they are part of the microbiota of raw materials, employed as starter cultures in numerous fermentation processes, and also play a role in spontaneous fermentation. Nevertheless, they have the pote...

  • Review
  • Open Access
1 Citations
4,119 Views
27 Pages

12 August 2025

Bacterial endophytes have emerged as critical components of plant microbiomes, offering multifaceted benefits ranging from growth promotion to stress resilience. This review synthesizes two decades of research, from 2004 to 2024, on bacterial endophy...

  • Review
  • Open Access
22 Citations
4,808 Views
29 Pages

Imaging Cultural Heritage at Different Scales: Part I, the Micro-Scale (Manufacts)

  • Luca Piroddi,
  • Nasser Abu Zeid,
  • Sergio Vincenzo Calcina,
  • Patrizia Capizzi,
  • Luigi Capozzoli,
  • Ilaria Catapano,
  • Marilena Cozzolino,
  • Sebastiano D’Amico,
  • Rosa Lasaponara and
  • Deodato Tapete

15 May 2023

Applications of non-invasive sensing techniques to investigate the internal structure and surface of precious and delicate objects represent a very important and consolidated research field in the scientific domain of cultural heritage knowledge and...

  • Review
  • Open Access
38 Citations
10,644 Views
12 Pages

Adipose and Muscle Cell Co-Culture System: A Novel In Vitro Tool to Mimic the In Vivo Cellular Environment

  • Palaniselvam Kuppusamy,
  • Dahye Kim,
  • Ilavenil Soundharrajan,
  • Inho Hwang and
  • Ki Choon Choi

24 December 2020

A co-culture system allows researchers to investigate the complex interactions between two cell types under various environments, such as those that promote differentiation and growth as well as those that mimic healthy and diseased states, in vitro....

  • Article
  • Open Access
26 Citations
1 Views
5 Pages

Modulation of Mice Fecal Microbiota by Administration of Casein Glycomacropeptide

  • Qingsen Chen,
  • Jinyi Cao,
  • Yuchen Jia,
  • Xueji Liu,
  • Yali Yan and
  • Guangchang Pang

21 December 2012

Casein glycomacropeptide (GMP) is known to promote the in vitro growth of Bifidobacteria and Lactobacilli. In this paper, we used conventional culture techniques and fluorescent in situ hybridization (FISH) techniques to investigate the effect of cas...

  • Article
  • Open Access
8 Citations
5,374 Views
18 Pages

Quantitative and Qualitative Image Analysis of In Vitro Co-Culture 3D Tumor Spheroid Model by Employing Image-Processing Techniques

  • Mukta Sharma,
  • Venkanagouda S. Goudar,
  • Manohar Prasad Koduri,
  • Fan Gang Tseng and
  • Mahua Bhattacharya

19 May 2021

This work proposes a novel region-estimation (RE) algorithm using the quantification of colon-cancer (HCT-8) and fibroblasts (NIH3T3) cells to estimate the densest region of colon-cancer cells in in vitro 3D co-cultured spheroids. Cells were labelled...

  • Project Report
  • Open Access
6 Citations
3,602 Views
16 Pages

28 December 2022

The actions for the preservation of cultural heritage must work on the identity and specificity of the places, paying the utmost attention to the context relationships. The analysis of these elements is fundamental to the recognition of the intrinsic...

  • Article
  • Open Access
2 Citations
2,817 Views
17 Pages

Multi-Technique Characterization of Cartonnage and Linen Samples of an Egyptian Mummy from the Roman Period

  • Francis Sanches,
  • Isis Franzi,
  • Josiane Cavalcante,
  • Roberta Borges,
  • Anderson de Paula,
  • Alessandra Machado,
  • Raysa Nardes,
  • Ramon Santos,
  • Hamilton Gama Filho and
  • Renato Freitas
  • + 4 authors

The historical and cultural significance of artistic works and archaeological artifacts underscores the imperative use of non-destructive testing methods in cultural heritage objects. Analyzing pigments in artwork poses a specific analytical challeng...

  • Article
  • Open Access
1 Citations
2,486 Views
15 Pages

β-carotene possesses antioxidant properties and holds significant research value. In our study, we have successfully identified a strain of Pantoea dispersa MSC14 which has the capability to produce β-carotene. By incorporating corn steep l...

  • Review
  • Open Access
240 Citations
34,958 Views
23 Pages

Advanced Cell Culture Techniques for Cancer Drug Discovery

  • Carrie J. Lovitt,
  • Todd B. Shelper and
  • Vicky M. Avery

30 May 2014

Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment. Research into dev...

  • Communication
  • Open Access
90 Citations
20,131 Views
14 Pages

Assessing MITRE ATT&CK Risk Using a Cyber-Security Culture Framework

  • Anna Georgiadou,
  • Spiros Mouzakitis and
  • Dimitris Askounis

9 May 2021

The MITRE ATT&CK (Adversarial Tactics, Techniques, and Common Knowledge) Framework provides a rich and actionable repository of adversarial tactics, techniques, and procedures. Its innovative approach has been broadly welcomed by both vendors and...

  • Article
  • Open Access
15 Citations
3,653 Views
21 Pages

Documentation and Virtualisation of Vernacular Cultural Heritage: The Case of Underground Wine Cellars in Atauta (Soria)

  • Tomás Ramón Herrero-Tejedor,
  • Miguel Ángel Maté-González,
  • Enrique Pérez-Martín,
  • Serafín López-Cuervo,
  • Juan López de Herrera,
  • Luis Javier Sánchez-Aparicio and
  • Paula Villanueva Llauradó

5 July 2023

The cultural heritage associated with rural systems is extremely valuable and is a sign of identity for cultures that are disappearing or undergoing transformation. This paper explains and illustrates the series of procedures that have been carried o...

  • Article
  • Open Access
6 Citations
4,348 Views
14 Pages

23 October 2021

The progression of time and shifts in the environment have brought significant changes to traditional arts and crafts of intangible cultural heritage. Consequently, enhancing and objectively assessing the sustainable value of traditional arts and cra...

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