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Advanced Cell Culture Techniques for Cancer Drug Discovery

Discovery Biology, Griffith University, N27, Don Young Road, Nathan, Queensland, 4111, Australia
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Biology 2014, 3(2), 345-367; https://doi.org/10.3390/biology3020345
Received: 24 February 2014 / Revised: 16 May 2014 / Accepted: 22 May 2014 / Published: 30 May 2014
(This article belongs to the Special Issue Screening for Biologically Active Compounds)
Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment. Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely characterizes the disease state have been undertaken by a number of laboratories around the world. These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor cells. In addition, a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two-dimensional monolayer conditions do not respond to cancer therapeutics/compounds in a similar manner. Recent studies have demonstrated the potential of utilizing 3D cell culture models in drug discovery programs; however, it is evident that further research is required for the development of more complex models that incorporate the majority of the cellular and physical properties of a tumor. View Full-Text
Keywords: 3D culture; microenvironment; drug resistance; tumor models; high-throughput screening 3D culture; microenvironment; drug resistance; tumor models; high-throughput screening
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Lovitt, C.J.; Shelper, T.B.; Avery, V.M. Advanced Cell Culture Techniques for Cancer Drug Discovery. Biology 2014, 3, 345-367.

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