Next Article in Journal
Mechanical and Metallurgical Evaluation of 3 Different Nickel-Titanium Rotary Instruments: An In Vitro and In Laboratory Study
Previous Article in Journal
Human-Origin iPSC-Based Recellularization of Decellularized Whole Rat Livers
Previous Article in Special Issue
Automated Analysis of Acetaminophen Toxicity on 3D HepaRG Cell Culture in Microbioreactor
 
 
Review

MatriGrid® Based Biological Morphologies: Tools for 3D Cell Culturing

1
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany
2
Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747 Jena, Germany
3
Biophysics Division, GSI Helmholtzzentrum für Schwerionenforschung, 64291 Darmstadt, Germany
4
Institute of Environmental Toxicology, Martin-Luther-University Halle-Wittenberg, 06097 Halle, Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Current address: Lonza AG, Quality Control Microbiology—Cleaning Validation, 3930 Visp, Switzerland.
§
Current address: Department of Bioelectronics (BEL), MINES Saint-Étienne, CEDEX 2, 42023 Saint-Étienne, France.
Academic Editors: Boštjan Vihar, Jan Rožanc and Uroš Maver
Bioengineering 2022, 9(5), 220; https://doi.org/10.3390/bioengineering9050220
Received: 8 April 2022 / Revised: 6 May 2022 / Accepted: 11 May 2022 / Published: 20 May 2022
(This article belongs to the Special Issue Analytical Approaches in 3D in vitro Systems)
Recent trends in 3D cell culturing has placed organotypic tissue models at another level. Now, not only is the microenvironment at the cynosure of this research, but rather, microscopic geometrical parameters are also decisive for mimicking a tissue model. Over the years, technologies such as micromachining, 3D printing, and hydrogels are making the foundation of this field. However, mimicking the topography of a particular tissue-relevant substrate can be achieved relatively simply with so-called template or morphology transfer techniques. Over the last 15 years, in one such research venture, we have been investigating a micro thermoforming technique as a facile tool for generating bioinspired topographies. We call them MatriGrid®s. In this research account, we summarize our learning outcome from this technique in terms of the influence of 3D micro morphologies on different cell cultures that we have tested in our laboratory. An integral part of this research is the evolution of unavoidable aspects such as possible label-free sensing and fluidic automatization. The development in the research field is also documented in this account. View Full-Text
Keywords: scaffolds for 3D cell culture; hepatocyte culture; scaffold manufacturing; manipulation of organoids; stem cell niches; neurons and cerebral bodies; automated cell culturing unit; 3D micropattern technique; microcontact printing; thermoforming; cell adhesion; extracellular matrix scaffolds for 3D cell culture; hepatocyte culture; scaffold manufacturing; manipulation of organoids; stem cell niches; neurons and cerebral bodies; automated cell culturing unit; 3D micropattern technique; microcontact printing; thermoforming; cell adhesion; extracellular matrix
Show Figures

Figure 1

MDPI and ACS Style

Mai, P.; Hampl, J.; Baca, M.; Brauer, D.; Singh, S.; Weise, F.; Borowiec, J.; Schmidt, A.; Küstner, J.M.; Klett, M.; Gebinoga, M.; Schroeder, I.S.; Markert, U.R.; Glahn, F.; Schumann, B.; Eckstein, D.; Schober, A. MatriGrid® Based Biological Morphologies: Tools for 3D Cell Culturing. Bioengineering 2022, 9, 220. https://doi.org/10.3390/bioengineering9050220

AMA Style

Mai P, Hampl J, Baca M, Brauer D, Singh S, Weise F, Borowiec J, Schmidt A, Küstner JM, Klett M, Gebinoga M, Schroeder IS, Markert UR, Glahn F, Schumann B, Eckstein D, Schober A. MatriGrid® Based Biological Morphologies: Tools for 3D Cell Culturing. Bioengineering. 2022; 9(5):220. https://doi.org/10.3390/bioengineering9050220

Chicago/Turabian Style

Mai, Patrick, Jörg Hampl, Martin Baca, Dana Brauer, Sukhdeep Singh, Frank Weise, Justyna Borowiec, André Schmidt, Johanna Merle Küstner, Maren Klett, Michael Gebinoga, Insa S. Schroeder, Udo R. Markert, Felix Glahn, Berit Schumann, Diana Eckstein, and Andreas Schober. 2022. "MatriGrid® Based Biological Morphologies: Tools for 3D Cell Culturing" Bioengineering 9, no. 5: 220. https://doi.org/10.3390/bioengineering9050220

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop