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Efficient co-utilization of mixed sugars from lignocellulosic hydrolysates is often hindered by carbon catabolite repression and pretreatment-derived inhibitors. In this study, a co-fermentation strategy using Saccharomyces cerevisiae (S. cerevisiae)...

In this research, cattle manure, macroalgae, and cheese whey were mixed in various proportions (cattle manure:macroalgae:cheese whey ratios of 50:30:20, 30:20:50 and 20:50:30) and subjected to co-digestion under laboratory conditions at two different...

The massive annual production of tea generates substantial underutilized by-products, leading to resource waste. This study aimed to develop an efficient process for converting these by-products into high-quality feed via fermentation with Lentilacto...

Background: Staurosporine is a potent broad-spectrum alkaloid antibiotic originally isolated from Streptomyces sp. It is renowned for its strong inhibitory activity against protein kinases by competitively binding to their ATP-binding sites. Therefor...

The increasing demand for alcohol-free craft beers with functional properties and distinctive sensory attributes has motivated the brewing industry to investigate alternative production strategies, such as the application of non-Saccharomyces yeasts,...

Yeasts isolated from fermented foods have attracted increasing attention for their probiotic potential; however, studies on yeast-derived postbiotics remain limited. In this study, endogenous yeast strains belonging to Kluyveromyces marxianus (n = 3)...

Mezcal is an ancestral beverage from Oaxaca, Mexico, produced using traditional biotechnological procedures without artificial inoculation. The role of bacteria in fermentation has been studied less. Our work aimed to analyze the bacterial diversity...

Lignocellulosic biomass is one of the most abundant renewable carbon resources available, currently used predominantly for energy generation through direct combustion, yet still underutilized as a feedstock for higher-value biochemical conversion. It...

Bacterial extracellular vesicles (BEVs) are nanosized (10–400 nm), membrane-enclosed particles naturally secreted by both Gram-negative and Gram-positive bacteria. Initially characterized as virulence factors in pathogenic species, BEVs are now...

Anaerobic digestion under a Waste-to-Energy (WtE-AD) framework represents a sustainable alternative for managing organic waste and generating bioenergy in developing countries. However, most life cycle assessment (LCA) studies implicitly assume stabl...

Coronaviruses are worldwide-distributed RNA viruses with zoonotic potential and the ability to jump from one host species to another, including humans. Even after the COVID-19 pandemic, the search for new, biologically active substances with anti-cor...

The complex polysaccharide pullulan is characterized as a glucose-containing biopolymer that is both water-soluble and neutral in polarity. A variety of commercial applications exist for pullulan, including its utilization as a flocculant, a blood pl...

Pulque is a traditional Mexican fermented beverage produced by the spontaneous fermentation of the sap (aguamiel) produced by several Agave (maguey) species. Pulque fermentation starts with the addition of freshly collected aguamiel (harvested twice...

The genus Rhodococcus is relevant for its biosynthetic capabilities and metabolic versatility, resulting in the production of different metabolites to adapt to harsh environmental conditions. Exopolysaccharides are secreted by different members of Rh...

Cupriavidus necator is a well-studied microorganism with potential application in bioregenerative life support systems for single-cell protein and bioplastic production. Most studies have been carried out in autotrophy or heterotrophy, requiring O₂ a...

This study focuses on the development of refrigerated doughs without chemical preservatives to obtain a clean-label product. Sourdough-based strategies were applied to replace conventional preservatives, using both spontaneous flour fermentation and...

Agave bagasse is a lignocellulosic residue generated after the extraction of fermentable sugars from agave hearts during tequila production. More than 0.5 million tons are generated annually, accumulating on a massive scale and posing a serious envir...

The selection of an optimal antifoam is critical for efficient fermentation, as industrial agents often have detrimental side effects like growth inhibition, while some can enhance productivity. We studied the efficacy of novel silicone–polyol...

A parallel-stirred tank bioreactor system on a 10 mL-scale automated with a liquid handling station introduces significant benefits in bioprocess analysis and design regarding preserving time, cost, and workload, thereby enabling quick generation of...

Peroxisomes are dynamic organelles involved in multiple metabolic pathways that respond to cellular and environmental conditions. Yeasts are a useful model for peroxisome studies, as their growth in media containing peroxisome proliferators, such as...

The family Lactobacillaceae, reclassified in 2020 into 25 genera comprising 261 species, remains one of the most extensively studied groups of lactic acid bacteria (LAB) due to its wide distribution in fermented products, commensal presence in the ga...

This study investigated the generation of α-glucosidase inhibitory peptides from whey protein fermented by the marine-derived probiotic Lacticaseibacillus casei DS31 (isolated from the intestinal microbiota of the large yellow croaker, Larimich...

The food industry generates large volumes of nutrient-rich by-products that remain underutilised despite their considerable biochemical potential. These materials originate predominantly from the fruit and vegetable, dairy, meat, and fish and seafood...

Fengycin, a lipopeptide synthesized by Bacillus species, exhibits pronounced antifungal activity; however, its low production titer remains a primary constraint to broader application. Global regulatory factors constitute key actionable targets for e...

Protists represent a vast yet underexplored reservoir of enzymatic diversity across the eukaryotic tree of life. In this study, we established axenic strains of diverse protists from four major eukaryotic supergroups using single-cell isolation and g...

Biological upgrading of biogas to biomethane is a promising power-to-gas technology with a low environmental footprint. However, due to the lower conversion rates, long-term investigations on mesophilic trickle-bed reactors (TBRs) remain scarce. This...

Intracellular metabolites markedly change in yeast during fermentation, especially under various stresses in beer post-fermentation. To address the current limitations in understanding the regulatory mechanisms in this complex environment, industrial...

The excessive use of chemical fertilizers raised concerns regarding environmental sustainability and soil degradation, prompting increasing interest in biofertilizers as eco-friendly alternatives. Among these, a compound that is effective in stimulat...

Daqu is the core saccharifying and fermenting starter for strong-flavor Baijiu, and its quality is strongly shaped by the workshop microenvironment. Here, mature Daqu from a newly built workshop and a long-established workshop within the same distill...

Chymosin is an aspartyl protease widely used in the food industry for milk coagulation during cheesemaking. Although recombinant production has replaced natural extraction from rennet, current heterologous expression systems still face significant ch...

The aims of this study were to evaluate the impact of probiotics (added as a starter sourdough and microcapsules) on gluten-free (GF) rice–amaranth steamed bread (SB) regarding physicochemical characteristics, sensory attributes, probiotic viab...

Excessive application of chemical fertilisers has led to soil phosphorus immobilisation and aquatic eutrophication, making the development of highly efficient acid/neutral phosphatases crucial for sustainable phosphorus utilisation. In this study, we...

Squalene, a hydrocarbon with several industrial applications, is obtained from plants, animals, and microorganisms. Oleaginous thraustochytrids are also potential sources of squalene. In eukaryotes, squalene, an intermediary in the sterol/cholesterol...

This study investigated the effect of fermentation time (24 and 48 h) on the pH, titratable acidity (TTA), phytochemicals, antioxidants, phenolic compounds, colour, functional, pasting, and thermal properties of flours from selected legumes (mung bea...

Corn fermentation in biorefineries produces residual biomass and by-products, particularly corn kernel fiber and outgassed carbon dioxide (CO₂), that have value-added potential for improving sugar and bioethanol conversions. Recovered corn kernel fib...

In this study, the effects of electric-field intensity on N transformation during aerobic composting of biochar/pig manure were investigated. Four experimental groups were established under different applied voltages: 0 V (Group CK); 2 V (Group L); 4...

The global shift toward antibiotic-free poultry production has created an urgent need for sustainable feed additives that promote gut health and productivity. This study aimed to develop and evaluate a novel double-layered microencapsulated phytosynb...

Fermentation technology has long been applied in animal nutrition worldwide, with its application primarily focused on animal feed [...]

Reducing the alcohol content of wines has received increasing attention, and various strategies have been proposed for this aim. In this study, non-Saccharomyces yeasts isolated from Uruguayan vineyards were screened to identify strains with low etha...

Cassava is a major starch crop in Africa, generating substantial amounts of solid (peels and fibres) and liquid (process press water) residues that remain underutilised, particularly in smallholder and semi-industrial processing units. In Mozambique,...

This study aimed to evaluate in situ ruminal nutrient degradation, fractional disappearance rate, and effective ruminal degradation of hydroponically sprouted barley, wheat, and triticale. Two ruminally canulated lactating cows were used in a complet...

Harnessing nature’s ingenuity with microorganisms for industrial production is an attractive solution to today’s climate concerns. Nature’s innate diversity allows the production of many value-added chemicals and can be expanded on...

Glucagon-like peptide-1 (GLP-1) is an incretin hormone and therapeutic agent for Type II diabetes mellitus. However, recombinant production in E. coli yields insufficient quantities, increasing manufacturing costs and limiting patient access. Improvi...

Fermented forest litter (FFL) is a biofertilizer obtained by anaerobic fermentation of forest litter combined with agricultural by-products. Its production involves an initial one-month solid-state fermentation of oak litter mixed with whey, molasses...

BslA (Biofilm surface layer protein A), a highly hydrophobic lipoprotein from Bacillus spp., self-assembles at fluid interfaces to form a crystalline film that reduces surface tension. In this study, we selected Pichia pastoris as a eukaryotic system...

The global prevalence of antibiotic-resistant bacteria, such as Staphylococcus aureus, presents a substantial challenge to public health, necessitating the development of innovative therapeutic strategies to combat these infections. This study examin...

Recent studies have shown that the use of non-Saccharomyces yeasts, either alone or in co-fermentation with Saccharomyces cerevisiae, can enhance the development of specialty beers with distinctive compositional characteristics. This study aimed to e...

Driven by the energy transition within the framework of the United Nations Framework Convention on Climate Change, second-generation (2G) ethanol stands out as a technical and sustainable alternative to fossil fuels. Although first-generation ethanol...

Silage is a core roughage resource for ruminant production, but aerobic deterioration caused by microorganisms severely reduces its nutritional value and increases microbial risk. This study aimed to isolate and identify specific spoilage organisms (...

Fermented sucuk is a fermented food product widely consumed and enjoyed by people in Türkiye. Spices and other additives are used in the production of fermented sucuk. Due to the increasing demand for natural and healthy food consumption, the ne...