Polymers, Volume 15, Issue 6
2023 March-2 - 264 articles
Cover Story: Enzyme-loaded submicrometer-sized polyion complex vesicles with tunable semi-permeability show high potential for enzyme prodrug therapy (EPT) as a nanoreactor. This nanoreactor is suitable for the local activation of prodrugs; however, it suffers from a low loading efficiency, which hinders practical applications. In this study, we developed a stepwise crosslinking (SWCL) method to improve the enzyme loading amount. Enzyme-loaded PICsomes prepared using the SWCL method resulted in several times higher enzyme loading—an increase in double figures—than that obtained through conventional methods. It also showed excellent antitumor efficacy and reduced side effects in tumor-bearing mice, confirming the significance of PICsome-based EPT. View this paper - Issues are regarded as officially published after their release is announced to the table of contents alert mailing list .
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