Search Results (4,026)

Seed coating technology is regarded as one of the optimal strategies to promote sustainable agricultural development. It can effectively optimize the physical and physiological characteristics of seeds, improve germplasm quality, and enhance crop resistance to abiotic and biotic stresses. Saline–alkali soils, characterized by high salinity and alkalinity, severely restrict plant growth and development. However, alfalfa, a high-quality leguminous forage, faces substantial challenges in large-scale popularization and cultivation in saline–alkali regions. At present, research on the application of microbial seed coating technology in alfalfa production under saline–alkali conditions remains insufficient, and relevant techniques and formulations still require optimization. Under field conditions, this study used a randomized complete block design with alfalfa as the research material. Different coating treatments combining plant growth-promoting rhizobacteria (PGPR), rhizobia, and extracellular polysaccharides (EPSs) were established to systematically investigate the effects of various coating formulations on alfalfa yield, nutritional quality, root system architecture, and rhizosphere soil properties. Meanwhile, high-throughput sequencing was employed to analyze shifts in rhizosphere soil microbial community structure. The results demonstrated that all microbial coating treatments exerted significant growth-promoting effects on alfalfa grown in saline–alkali soils, among which the T8 treatment (combined coating of rhizobia + PGPR + EPS) performed the best. This treatment not only significantly improved alfalfa yield and nutritional quality but also modified root system architecture and enhanced soil enzyme activities, soil nutrient contents, and soil physical structure, thereby creating a favorable growth environment for plants. Among the single microbial coating treatments, the combined coating of rhizobia and EPS outperformed other single treatments and exhibited favorable application potential. Sequencing results revealed that microbial seed coating treatments significantly increased the relative abundance of beneficial soil bacteria, decreased the abundance of harmful fungi, regulated rhizosphere microbial community structure, and consequently promoted improvements in alfalfa yield and quality by optimizing the plant growth microenvironment. The findings of this study provide important theoretical support for the popularization and application of microbial seed coating technology in crop cultivation in saline–alkali soils, offer a key reference for optimizing alfalfa-specific seed coating formulations for saline–alkali conditions, and are of great significance for promoting the efficient utilization of saline–alkali land resources and the development of ecological agriculture. Full article

This study investigated the combined effects of microbial-inoculated biochar and nitrogen (N) on rice growth and soil properties under saline conditions. A randomized complete block design with three replications was employed to evaluate three factors: (i) salinity level (non-saline, S0; saline, 0.4% NaCl, S1), (ii) biochar type (20 t/ha BC, BF, BB, and BFB), and (iii) nitrogen application rate (60 and 120 kg ha⁻¹). Soil physicochemical and biological properties, along with rice root development, were assessed. Salinity significantly reduced soil organic matter (OM) by 9%, nitrate nitrogen (NO₃⁻-N) by 16%, ammonium nitrogen (NH₄⁺-N) by 8.18%, and available phosphorus (AP) by 6.81%. Soil enzyme activities, including catalase (CAT), acid phosphatase (ACP), polyphenol oxidase (POX), and β-D-glucosidase (BG), decreased by 32.69%, 29%, 39.18%, and 19.44%, respectively, resulting in suppressed root growth compared with non-saline conditions. The combined treatment of microbial biochar (BFB) and N at 120 kg ha⁻¹ (BFB + N120) markedly improved saline soil quality and rice root performance by maintaining a favorable K⁺/Na⁺ balance in roots. Specifically, BFB+N120 increased OM by 145% and 120% compared with N120 and BC alone, respectively, and enhanced NO₃⁻-N, NH₄⁺-N, and soil enzyme activities (CAT, ACP, POX, and BG). These improvements were strongly associated with enhanced root development. Under saline conditions, BFB+N120 increased root dry mass by 429% and 1185.71%, and root length by 63% and 83%, compared with N120 and BC alone, respectively, in the cultivar Jing Liang You 534. Overall, the results demonstrate that microbial-modified biochar combined with nitrogen fertilizer mitigates salt-induced soil degradation by improving physicochemical and biological properties, thereby enhancing nutrient availability, ionic homeostasis, and root growth. This study provides mechanistic insights into the combined role of microbial biochar and nitrogen in the remediation of saline soils. Full article

Approximately 20% of China’s land area is desertified or highly desertifiable, where loose sandy soil and low nutrient availability restrict plant growth. Microbial inoculants, as an emerging ecological restoration technology, play a key role in plant growth and soil nutrient activation in sandy regions. However, a systematic understanding of functional differences among microorganisms isolated from different stressed environments remains insufficient. Nine functional microbial strains from three stressed habitats, including sandy land, coastal saline-alkali soil, and heavy metal mining areas, were selected to conduct a three-month pot experiment, investigating their effects on soil nutrient activation, plant growth and microbial communities. Results showed that all inoculants increase plant biomass (by 4.15~25.59%), with KS-33, KS-36, SD-13 and SD-3 significantly promoting biomass in different plant parts (p < 0.05), and with YJ-15 remarkably enhancing root growth (root length increased by 70.83%, p < 0.01). Inoculation reduced bacterial Chao1 by 27.18~53.97%, but increased fungal Chao1 by 12.77~28.38% (except SD-30). Bacterial generalist species proportion increased from 61.12% to 83.78~93.99% after inoculation, higher than the variation degree of the fungal community. Mantel analysis revealed a reverse trend between soil nutrients, water content and plant growth. This may be associated with the increased consumption by plants and microorganisms. In summary, microbial inoculants enhance nutrient cycling processes and plant growth by reshaping soil microbial communities. Performance of microbial inoculants is more likely governed by their inherent ecological functions rather than being entirely determined by their original environments. Despite varying mechanisms, these inoculants can effectively enhance sandy soil microbial communities, providing a theoretical basis for regional ecological restoration. Full article

Soil salinity severely threatens global ecosystems and agriculture, making accurate monitoring an ongoing priority. Currently, efficiently utilizing multi-source datasets to enhance monitoring accuracy while minimizing computational resources remains a critical challenge. This study evaluated several modeling strategies, including full-dataset modeling, variance inflation factor (VIF), Boruta, particle swarm optimization, ant colony optimization and recursive feature elimination (RFE), and validated results across diverse regions (Almaty, Kazakhstan; Shandong, China). We further validated the results using multiple algorithms, including linear regression, partial least squares regression, extreme gradient boosting, k-nearest neighbor and random forest (RF), with topsoil (0–20 cm) electrical conductivity inverted via the optimal method. Results indicate that input feature numbers substantially impact model performance: regional-scale feature selection is indispensable, with RFE outperforming full-dataset modeling (R² improves by up to 0.28, while RMSE decreases by 2.21 dS m⁻¹) and VIF performing the worst. Transferability is also demonstrated in Almaty and Shandong. Additionally, the RF algorithm shows superior performance in soil salinity mapping (overall accuracy = 0.73; kappa coefficient = 0.65). And, the RFE and SHAP results highlight CRSI, BI, and MSAVI2 as particularly important predictors for estimating soil salinity in our study area. Collectively, this study highlights the critical importance of feature optimization and interpretability in soil attribute mapping through the integration of multi-source remote sensing data. Full article

Groundwater is a strategic resource for maintaining ecological balance and supporting human development in arid inland basins. However, under the dual pressures of climate change and human activities, it faces threats in both quantity and quality. This study selects the Chahan Usu River watershed in the eastern Qaidam Basin, a typical arid inland basin on the Tibetan Plateau, to assess the current quality of groundwater resources and reveal the formation mechanisms and material sources of its hydrochemistry. The results show that the groundwater in the watershed is generally weakly alkaline, with some areas exhibiting high salinity. The dominant cations and anions are Na⁺ and Cl⁻, respectively. The hydrochemical type is mainly Cl-Na, with a minority being mixed Cl-Mg·Ca. Overall, the groundwater in the watershed is suitable for domestic use. However, in the middle and lower reaches of the Chahan Usu River, nitrate and ammonia nitrogen contamination reduce its suitability. Meanwhile, although long-term use of this groundwater would not lead to soil degradation, its widespread high salinity and high sodium content make it unsuitable for irrigation. Water–rock interactions with evaporites and silicate rocks are the main mechanisms controlling groundwater chemistry in the watershed. Among them, halite minerals contribute most of the Na⁺ and Cl⁻, while sulfate minerals provide Ca²⁺ and SO₄²⁻. In addition, cation exchange is widespread. This study provides a reference for ensuring the security and sustainable development of groundwater resources on the plateau. Full article

Saline–alkali soils suffer from severe deficiencies in available phosphorus, and externally added phosphorus is readily immobilized by metal ions in the soil. Therefore, activating inorganic phosphorus in the soil represents a significant challenge. In this study, 35 salt–alkali-tolerant bacteria were isolated from rhizosphere soils (pH 9.20–9.68). Three phosphate-solubilizing strains (HA2, HPA5, and KA1) capable of growing under severe saline–alkali stress conditions (pH 10, 5% NaCl) and possessing multiple plant growth-promoting traits (nitrogen fixation, potassium solubilization, siderophore production, and IAA secretion) were screened and co-cultured to form the microbial consortium HHK. It was hypothesized that this consortium might exhibit synergistic effects, resulting in significantly higher phosphorus solubilization capacity compared to individual strains. The results showed that under saline–alkali stress, the phosphate solubilization capacity of HHK (484.59 ± 15.79 mg/L) was significantly higher than that of any single strain (285.59 ± 12.60 mg/L). Non-targeted metabolomics and enzyme assays indicated that HHK solubilizes P via organic acids (e.g., citric, L-malic acid) and synergistically modulates core metabolic pathways, including ABC transport, TCA cycle, and glycolysis, alleviating oxidative damage and maintaining cellular homeostasis. Short-term soil incubation confirmed that HHK significantly increased available phosphorus (53.67%) and soil fertility, indicating its potential as a biofertilizer. Full article

This paper analyses the impact of climate change on fruit species, synthesizing evidence of how abiotic stresses—such as extreme temperatures, drought, salinity, and water fluctuations—influence the physiology, metabolism, phenology, and productivity of fruit trees. It examines both direct effects on flowering, fruit set, growth, and quality, as well as indirect impacts on nutrient availability, soil health, and vulnerability to pests and diseases. The article highlights the role of hormones and secondary metabolites in mediating stress responses, alongside the critical importance of cellular and antioxidant protection mechanisms. Adaptive strategies across physiological, biochemical, molecular, and agronomic levels are discussed, including the selection of tolerant varieties and rootstocks, irrigation adjustments, microclimatic management, and the use of biotechnological approaches and biostimulants to enhance fruit resilience and quality. In conclusion, the article underscores the necessity of an integrated approach to ensure the sustainability and productivity of orchards in the face of climate change. Full article

Soil salinization severely threatens agricultural sustainability in saline–alkali regions, and high-throughput, efficient screening of salt-tolerant rice varieties is critical to mitigating this threat. Traditional evaluation methods are constrained by low throughput, limited spatiotemporal resolution, and the lack of standardized indicators. To address these gaps, this study established a multi-scale spectral phenotyping framework integrating ground-based hyperspectral, UAV-borne multispectral, and Sentinel-2 satellite remote sensing data for high-throughput screening of salt-tolerant rice. Field experiments were conducted with 12 rice lines at five key growth stages in Ningxia, China, with synchronous ground spectral measurements and UAV image acquisition on the same day for each stage. Five feature selection methods were employed to screen salt stress-sensitive hyperspectral bands, with classification accuracy validated via a Support Vector Machine (SVM) model. The results showed that: (1) rice spectral characteristics varied dynamically across growth stages, and first-order differential transformation effectively amplified subtle spectral variations in stress-sensitive regions; (2) the Minimum Redundancy–Maximum Relevance (mRMR) method outperformed other methods, achieving 100% classification accuracy at key growth stages, with sensitive bands dominated by red edge bands (58.33%); (3) the constructed Salt Stress Index (SIR) showed strong correlations with classical vegetation indices and rice yield, and could clearly distinguish salt-tolerant and salt-sensitive rice varieties, with stable performance against field environmental noise; and (4) band matching between UAV and Sentinel-2 data enabled multi-scale data fusion and regional-scale salt stress monitoring. This framework realizes the transformation from qualitative spectral description to quantitative salt tolerance evaluation, providing standardized technical support for salt-tolerant rice breeding and precision management of saline–alkali lands. Full article

In irrigated areas around the world, the recommendation for the use of subsurface drainage is also associated with controlling salinity problems. Due to the high implementation cost, the search for solutions that make this requirement more flexible is necessary. Among the options to be investigated is the hypothesis that the height and salinity of the water table in plots located at the highest points of a toposequence are lower and do not compromise plant development, even without underground drainage systems. In this context, the present work was developed to monitor and evaluate the variation in water level or mottling over twelve months, as well as to measure and analyze the electrical conductivity and average pH of the water table during this period and its possible impact on plants. For this purpose, three lots in toposequence were selected in the Senador Nilo Coelho Public Irrigation Project, Petrolina—PE, with previously defined characteristics: soil classification (Plinthic Yellow—Ultisol), crop planted (Mangifera indica L.) and irrigation system used (micro-sprinkler). Precipitation, reference evapotranspiration and volume of water applied via irrigation were monitored by an automatic weather station and hydrometers in each lot. In each plot, nine observation wells were installed, distributed in a grid, with the aim of make monthly measurements of the water table level or mottling. The electrical conductivity and pH of the groundwater were also measured to obtain the average monthly value for each lot. Illustrative 3D maps of the water table level in relation to the ground surface were created using the simple kriging method, in the UTM SIRGAS 2000 24S projection system. The absence and presence of groundwater in the upper and lower hillslope lots, respectively, were favored by the toposequence. The decision to install underground drainage or not can be made on a case-by-case basis; this must take into account, among other aspects, changes in physical characteristics along the soil profile, possible occurrence of mottling, the quality of water for irrigation, the irrigation management adopted and the position of the lot in the toposequence. Full article

This Special Issue, entitled “Digital Soil Mapping for Agri-Environmental Management and Sustainability”, gathers nine studies from around the globe that illustrate how digital soil mapping (DSM) is being applied to support agri-environmental management and sustainability. Field- and farm-scale studies are emphasized, where informed decisions are essential for efficient day-to-day management and profitability. The articles highlight the integration of remote/proximal sensing, along with modern machine learning techniques, to produce high-resolution soil maps, soil fertility and nutrient management zoning, and to monitor salinity and soil moisture to inform irrigation and land management. Another key focus is improving sampling strategies and assessing prediction uncertainty and model interpretability. This collection sets future DSM priorities, including cost-effective sampling, robust uncertainty assessments, and reliable cost–benefit and risk assessment approaches that link map accuracy/uncertainty to management outcomes and economic performance. Full article

Due to limited freshwater availability for winter wheat and summer maize, grain production in the annual double-cropping system of the low plain surrounding the Bohai Sea in North China is strongly influenced by inter-annual rainfall variability. The relatively abundant saline water resources in this region offer a potential source for irrigation. This study aimed to evaluate the effects of additional saline water irrigation under deficit irrigation on the crop yields and water productivity of winter wheat and its following crop maize, as well as to determine the soil salinity dynamics and annual salt balance under saline irrigation. A two-year field experiment (2023–2025) was conducted using six irrigation treatments, namely rainfed (I0), one freshwater irrigation (If), one saline irrigation (Is), combinations of freshwater and saline irrigation (Is + If, If + Is), and two freshwater applications (If2) to evaluate the effects of an additional saline water irrigation event, compared with the commonly used freshwater irrigation regime, on crop yields, water productivity, and the soil salt balance. The results showed that a single saline irrigation event (70 mm) increased the wheat yield by 18–38% under rainfed conditions and by 7–10% under limited freshwater irrigation. In contrast, the maize yield was not affected by the additional saline irrigation applied during the winter wheat season. Although salt accumulation occurred in the topsoil following the saline irrigation of winter wheat, it did not impair maize growth, owing to salt leaching during irrigation for maize emergence and concentrated summer rainfall. Within the two-year observation period, no progressive salt accumulation was observed in the top 1 m soil profile. These findings indicate that the strategic use of saline water to supplement the crop water supply can enhance crop production under deficit irrigation, provided that soil salinity is effectively managed. Full article

Soil salinization and heavy metal pollution represent significant global challenges to farmland sustainability and food security. Globally, over 800 million hectares of land are affected by salinity, with approximately 17% of cultivated land exhibiting concentrations of at least one heavy metal exceeding established agricultural safety thresholds. Microbially Induced Calcium Carbonate Precipitation (MICP) is an innovative biogeochemical process that harnesses microbial metabolic activities to facilitate soil mineralization. The core mechanism involves ureolytic microorganisms hydrolyzing urea to produce carbonate ions (CO₃²⁻). These ions subsequently react with environmental calcium ions (Ca²⁺) to form insoluble calcium carbonate (CaCO₃) precipitates. This review synthesizes recent research progress on the application of MICP technology for the remediation of heavy metal pollution. It elucidates the mechanistic pathways by which MICP immobilizes heavy metal ions and critically evaluates its potential application for ameliorating heavy metal contamination specifically within saline–alkaline soils. Key challenges impeding the broader practical deployment of MICP are analyzed, particularly concerning salt-alkali stress tolerance and the management of ammonia emissions during urea hydrolysis. Emerging strategies, such as the synergistic integration of MICP with biochar amendments, offer promising solutions. Biochar can provide a protective microenvironment for microbial consortia and potentially mitigate ammonia volatilization, thereby enhancing the overall efficacy and feasibility of this remediation approach for contaminated saline–alkaline lands. Full article

Fungal endophytes are microorganisms that inhabit plant tissues without causing disease and emerge as critical mediators of plant stress tolerance, nutrient acquisition, and ecosystem resilience under diverse climate change scenarios. Their unique position within the host allows them to modulate physiological responses more closely than external microbiota. This review explores how endophytic fungi contribute to plant adaptation under climate-induced stresses such as heat, salinity, drought, pollution, and nutrient limitation, with a focus on molecular crosstalk, functional trait modules, and metabolic trade-offs. Key findings emphasize multilayered signaling systems, including MAMP/DAMP recognition, phytohormone regulation, immune tuning, ROS dynamics, and effector deployment, while emerging mechanisms such as cross-kingdom RNA and extracellular vesicle (EV)-mediated exchange are discussed as promising but currently limited in empirical validation within many endophytic systems. Endophytes also enhance nutrient exchange through conditional carbon-for-benefit trade and may shape rhizosphere microbiota and soil activities through plant-mediated inputs. Integrative multi-omics approaches provide predominantly correlational insights into the mechanistic basis of these effects, linking molecular function to ecosystem and community outcomes. These insights have potential applications in climate-resilient agriculture, phytoremediation, and ecosystem restoration; however, their large-scale implementation requires further field-based validation and context-specific assessment. Future priorities should focus on trait-based selection, ecological modeling, and biosafety evaluation to translate microbial functions into reliable field-level strategies that support sustainable crop performance under accelerating environmental stress. Full article

In permanently submerged coastal wetlands, interactions between biogeochemical processes and microbial communities strongly influence greenhouse gas (GHG) fluxes. To improve our understanding of how redox-driven processes shape GHG dynamics in these ecosystems, we investigated the relationships among iron (Fe) pools, microbial dynamics, and the potential GHG production in subaqueous soils from an interdunal wetland in San Vitale Park (Italy), permanently submerged and affected by seasonal oscillations of the saline water table. Two subaqueous soil columns (WAS-2 and WAS-4), collected from similar settings, were analyzed. Surface layers of WAS-4 showed higher salinity and carbonate content, whereas WAS-2 was characterized by overall higher Fe concentrations. Distinct vertical distributions of organic matter and sulfur (S) were shown along depth. Laboratory incubations revealed that nitrous oxide (N₂O) production was up to ten times higher in WAS-2 than in WAS-4, with peaks in the top 13–14 cm, consistent with more active nitrification-denitrification in surface layers. Methane (CH₄) and carbon dioxide (CO₂) fluxes decreased with depth, reflecting reduced availability of labile carbon. Methanomicrobiales dominated CH₄-producing layers, indicating hydrogenotrophic methanogenesis, while amoA-carrying Nitrosomonadales and Thaumarchaeota, occurred in shallow, organic-rich layers where ammonia supported nitrification and denitrification. Denitrifiers mainly belonged to α- and β-Proteobacteria, consistent with their direct contribution to N₂O peaks. Spearman’s correlations showed N₂O positively correlated to sulfur and labile carbon (C), supporting denitrification under moderately reducing conditions. CH₄ and CO₂ positively correlated with organic C (Corg), total nitrogen (TN), and reactive Fe forms, reflecting redox-mediated microbial respiration and methanogenesis. Trace elements (B, Cr, Cu, Ni) acted as micronutrients or inhibitors depending on concentration. Canonical correspondence analysis indicated depth-structured links among gas fluxes, soil chemistry (Corg, TN, S/C, CaCO₃, P), and microbial distributions: surface layers, rich in labile C and nutrients, supported active bacteria and archaea involved in decomposition, nitrification, and denitrification, whereas deeper layers hosted oligotrophic archaea adapted to inorganic substrates. Overall, Fe pools appeared to be associated with soil processes relevant to GHG dynamics, although the extent of their regulatory role remains uncertain due to potential alterations of redox-sensitive Fe fractions during sample handling. These results contribute to broader efforts to predict GHG emissions in submerged wetland soils by linking redox stratification, inorganic chemistry, and microbial functional groups. Full article

Soil salinization is a major constraint on sustainable agriculture worldwide, highlighting the need for stress-tolerant plant growth-promoting rhizobacteria (PGPR) for salt-affected soils. A moderately halophilic and alkali-tolerant bacterium, Staphylococcus succinus NYN-1, was isolated from the rhizosphere soil of the halophyte Suaeda dendroides collected from a highly salinized site in Xinjiang, China. This study aimed to evaluate its salt–alkali tolerance and plant growth-promoting potential through integrated phenotypic characterization, pot experiments, and whole-genome analysis. NYN-1 grew over a broad salinity range [0–15% (w/v)] and pH range (6.0–11.0), and showed plant growth-promoting activities including organic phosphorus mineralization, inorganic phosphate solubilization, potassium solubilization, and NH₄⁺ production. In pot experiments under 300 mM NaCl, inoculation with NYN-1 significantly improved the growth performance of maize (Zea mays L.), cotton (Gossypium hirsutum L.), and sunflower (Helianthus annuus L.). Genome analysis identified multiple Na⁺/H⁺ antiporter-related genes and genes encoding compatible-solute transport systems that are consistent with adaptation to salt–alkali stress. The genome also harbors a broad set of genes related to phosphorus metabolism, as well as other plant growth-promoting functions, including potassium solubilization-related pathways and siderophore biosynthesis. Collectively, these findings identify S. succinus NYN-1 as a promising native halophilic PGPR candidate and a potential microbial resource for developing inoculant strategies in salt-affected agricultural systems. Full article