Search Results (10,492)

Background: The high incidence and severe health threat of Toxoplasma gondii (T. gondii) infection, particularly in immunocompromised patients, underscore the urgent need for the development of a safe and effective vaccine. The aim of this study was to develop a novel multi-epitope vaccine (USM.TOXOII) incorporating the T. gondii GRA14, SAG1, and GRA1 antigens, and to assess its immunogenicity in BALB/c mice. Methods: Using bioinformatics approach, the USM.TOXOII was designed and evaluated. The encoding gene (471 bp) was then constructed and cloned into the pET-30a (+) plasmid before being transformed into E. coli expression system. The recombinant USM.TOXOII protein was subsequently expressed and purified. Finally, an animal study was performed to assess the vaccine’s immunogenicity. Results: The USM.TOXOII protein (17.27 kDa) was soluble and contained a His tag protein. Immunization of BALB/c mice with USM.TOXOII significantly elevated serum levels of total IgG, IgG1, and IgG2a (p < 0.05). Cytokine analysis revealed a significant increase in IFN-γ production, whereas IL-4 levels remained unchanged, suggesting a Th1-biased immune response. Conclusions: Collectively, these findings indicate that USM.TOXOII possesses immunogenic potential and is capable of inducing both humoral and cellular immune responses in BALB/c mice. Future challenge studies with live T. gondii tachyzoites are warranted to evaluate its protective efficacy in vivo. Full article

Background and Clinical Significance: Many diagnostic radiopharmaceuticals are excreted in the urine. This can pose a diagnostic challenge when urine-containing structures are in atypical locations, particularly in review of planar imaging without anatomical details from cross-sectional imaging. This case highlights a challenging ^99mTc-methylene diphosphonate (^99mTc-MDP) bone scan in a patient with an inguinal hernia containing a portion of the urinary bladder. Subsequently, we review diagnostic challenges on conventional and molecular imaging following surgical repair of the inguinal hernia. Case Presentation: A 79-year-old man with prostate cancer underwent initial staging prior to prostatectomy with ^99mTc-MDP bone scintigraphy. Anterior and posterior images showed focal uptake overlying the pubic symphysis. Lateral views showed that the activity was extraosseous. Follow-up CT urography showed a bladder hernia as the cause of the abnormality on bone scan. Prostatectomy and inguinal hernia repair were performed as a combination case. Four years postoperatively, follow-up ⁶⁸Ga-PSMA-11 positron emission tomography/computed tomography (PET/CT) showed no recurrence. The CT component of the exam showed an intermediate-density focus at the right inguinal hernia repair site, corresponding to a plugoma related to a polypropylene mesh plug, and a hyperattenuating Gore-Tex mesh repair of the left inguinal hernia. Conclusions: This case highlights the importance of lateral projections in resolving scintigraphic pitfalls and recognizing mesh-related imaging appearances to prevent misinterpretation. Full article

β-Galactosidase is an important enzyme for lactose hydrolysis because it catalyzes the conversion of lactose into glucose and galactose. In this study, Aspergillus niger C18, which showed β-galactosidase-producing ability during preliminary screening, was selected as the gene source. A β-galactosidase gene from this strain was cloned into the pET28a vector and heterologously expressed in Escherichia coli. Solid-state fermentation conditions were optimized to produce the native enzyme as a reference for comparison. The enzymatic properties of the recombinant enzyme were then systematically characterized and compared with those of the native enzyme. The recombinant β-galactosidase exhibited favorable thermal and pH stability. After incubation for 2 h at its optimal pH and optimal temperature, the recombinant enzyme retained 88.9% and 94.1% of its initial activity, respectively; specifically, 88.9% corresponded to pH stability and 94.1% corresponded to thermal stability. These results indicate favorable stability of the recombinant enzyme under the tested conditions. Thin-layer chromatography and high-performance liquid chromatography analyses confirmed that the recombinant enzyme efficiently hydrolyzed lactose in a model lactose solution, achieving more than 99.0% lactose degradation after 12 h of reaction. These findings suggest that β-galactosidase derived from A. niger C18 is a promising candidate for lactose hydrolysis. Full article

Background/Objectives: Differentiating recurrent disease from postsurgical changes on ¹⁸F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) remains challenging in malignant pleural mesothelioma (MPM). This study aimed to characterize the temporal patterns of postsurgical FDG uptake and evaluate the diagnostic performance of FDG PET/CT for detecting recurrent disease after radical surgery. Methods: We retrospectively analyzed 91 postsurgical PET/CT scans from 45 patients with MPM who underwent extrapleural pneumonectomy (EPP; n = 29) or pleurectomy/decortication (P/D; n = 16). Scans were stratified into four postoperative time intervals: <6 months, 6 to <12 months, 12 to <24 months, and ≥24 months. FDG uptake in the postsurgical bed and local recurrent lesions was quantified using maximum standardized uptake value ratios normalized to the mediastinal blood pool and liver. Recurrence was confirmed by histopathology or follow-up imaging. Results: Postsurgical FDG uptake showed a time-dependent decline, with significantly lower uptake beyond 24 months postoperatively (p < 0.05). EPP patients demonstrated significantly higher postsurgical FDG uptake than P/D patients (p < 0.01). FDG PET/CT identified occult recurrence in 23.4% of CT-negative scans. Local recurrent lesions showed significantly higher FDG uptake than postsurgical changes across all postoperative intervals (p < 0.001). Conclusions: Postsurgical FDG uptake in MPM demonstrates a time-dependent decline, and surgical extent is an important determinant of background metabolic activity. Despite this variable background, FDG PET/CT demonstrated high diagnostic accuracy for detecting recurrent disease, including CT-occult recurrences. Incorporating surgical type and postoperative interval into PET/CT interpretation may improve diagnostic accuracy in postoperative MPM surveillance. Full article

For students in animal science-related disciplines, animal welfare education may be associated with their understanding of human–animal relationships and willingness to pursue animal-related careers. However, empirical research from developing countries such as China remains limited. To address this gap, we conducted a survey among undergraduates at Northeast Agricultural University to examine the associations of demographic characteristics, educational exposure, and animal-related experiences with animal welfare awareness and career aspirations. A total of 346 valid responses were obtained. The results showed that students who encountered animal welfare through school demonstrated significantly higher levels of self-reported animal welfare awareness (p < 0.001). Binary logistic regression further confirmed that the channel remained significantly associated with the level of awareness (OR = 8.714, p < 0.001). Furthermore, gender and pet-keeping experience were significantly associated with career aspirations in both univariate and logistic regression analysis. In addition, although 50.00% of respondents considered primary school to be the optimal stage for animal welfare education, 81.48% of those exposed through school channels reported their first exposure at university. These findings highlight the need for a progressive animal welfare education framework, transitioning from life education and human–animal relationship training at the primary level to professional practice education in higher education. This approach may help students develop more stable and scientifically informed understanding of animal welfare, as well as a stronger sense of professional identity. Full article

Waste-to-energy (WtE) systems are frequently proposed as complementary waste-management strategies; however, their climate performance depends on the interaction between thermodynamic efficiency, material circularity, and electricity-system characteristics. Existing life-cycle assessments generally provide static comparisons between landfill and WtE but rarely identify the operating conditions under which WtE remains environmentally competitive. To address this gap, a parametric life cycle–energy framework was developed by integrating attributional LCA with an analytical energy model capable of evaluating critical efficiency thresholds under varying recovery rates and electricity-grid conditions. Four representative thermoplastics (PET, HDPE, PP, and LDPE) were evaluated using ReCiPe 2016 Midpoint (H) in SimaPro under Mexican electricity conditions ($E{F}_{\mathrm{grid}}=0.444$ kg CO₂eq/kWh). Results indicate that total life-cycle climate impacts are dominated by upstream polymer production, whereas end-of-life management contributes only marginally to overall GWP. Critical-efficiency analysis revealed strong sensitivity to both recovery rate and electricity-grid carbon intensity. For PET, the minimum efficiency required for WtE to outperform landfill increased from 13.1% to 73.5% across the evaluated scenarios, whereas HDPE remained competitive at efficiencies below 1.3%. Monte Carlo simulations (10,000 realizations) further demonstrated that avoided emissions decline systematically with increasing recovery rates, with LDPE exhibiting the highest mean avoided emissions (1735 kg CO₂eq) and PET the lowest (811 kg CO₂eq). These results demonstrate that WtE climate performance is governed primarily by residual waste availability and electricity-system evolution rather than thermodynamic efficiency alone. Consequently, WtE should be interpreted as a transitional residual-waste management strategy whose long-term climate relevance decreases as material circularity and electricity-grid decarbonization advance. Full article

Background: A significant proportion of individuals with Takayasu arteritis (TA) experience relapses notwithstanding standard treatment with glucocorticoids, and conventional synthetic or biologic disease-modifying antirheumatic drugs (DMARDs). As the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway contributes to the pathogenesis of TA, JAK inhibitors (JAKi) could represent a viable therapeutic alternative. This study assessed the effectiveness of JAKi in patients with relapsing TA within a real-world setting in a country with a low incidence of TA such as Spain and included a comprehensive review of the literature. Methods: we conducted a retrospective analysis of TA patients managed with JAKi for recurrent disease across three Spanish centers. Evaluated outcomes comprised clinical remission, clinical and analytical remission, glucocorticoid-sparing effect, improvement in imaging techniques, and adverse events. A systematic literature search was performed to identify further cases of TA treated with JAKi. Results: six patients (83.3% females) with a mean age 48.5 years and relapsing TA received JAKi therapy: baricitinib (n = 2); tofacitinib (n = 2), and upadacitinib (n = 2). Before JAKi therapy, all (100%) patients had received conventional synthetic immunosuppressants, and four (66.7%) biologics. Clinical remission was achieved in 2/6 (33.3%), 3/5 (60%), 3/5 (60%), 2/3 (66.7%), and 2/2 (100%) patients at 1, 3, 6, 12 and 18 months, respectively. Clinical + analytical remission was observed in 1/6 (16.7%), 2/5 (40%), 2/5 (40%), 2/3 (66.7%), and 2/2 (100%) patients, respectively. Two patients who underwent a follow-up PET/CT imaging showed partial improvement in both. After a median (IQR) follow-up of 9.5 (6.0–16.7) months, one (16.7%) patient discontinued the initial JAKi due to no improvement and one patient discontinued it because was diagnosed with tonsillar neoplasia. The literature search identified another 166 JAKi-treated TA cases with clinical improvement reported for the majority of them. Conclusions: this real-world analysis and literature review suggest that JAKi could be effective in the management of TA, including for those patients who have failed established glucocorticoid-sparing strategies. Full article

In this study, twin screw extruded Polyetherimide (PEI)/Poly(ethylene terephthalate) (PET) polymer blends (90/10, 70/30, 50/50 w/w%) were investigated to elucidate the composition–property relationship governed by morphological, structural, rheological, thermomechanical, mechanical, and electromagnetic shielding (EMI) performance behavior. Among other polymer blends, the 70/30 blend exhibits superior thermomechanical stability with a significant glass transition temperature of 132.7 °C, where a robust confinement effect effectively restricts the mobility of PET chains. This morphology, characterized by a domain size of 562 nm, provides proof of concept for interface-driven attenuation, reaching a maximum EMI shielding effectiveness of 2.54 dB within the investigated blends. This performance is primarily governed by Maxwell–Wagner–Sillars polarization at the immiscible boundaries, alongside an optimized dielectric loss of tan δ ≈ 0.065. The design of these high-temperature PEI blends provides a proof of concept for interface-driven attenuation and demonstrates their potential for developing advanced EMI shielding matrices. Full article

Glycolysis of poly(ethylene terephthalate) (PET) offers a promising route for chemical recycling, yet conventional homogeneous catalysts often suffer from low selectivity, severe side reactions (especially diethylene glycol, DEG formation), and detrimental metal residues that compromise the quality of recycled products. To address these challenges, we herein develop dipotassium phenylphosphonate (PPOA-K) as an efficient homogeneous catalyst for PET glycolysis. Under optimized conditions (1 wt% catalyst, 197 °C, EG/PET mass ratio 3:1, 90 min, atmospheric pressure), PPOA-K achieves 100% PET depolymerization and a high BHET yield of 86.0%, and the reaction follows apparent first-order kinetics with an activation energy of 70.3 kJ·mol⁻¹. Beyond its high catalytic activity, PPOA-K effectively suppresses the acid-catalyzed etherification of ethylene glycol to DEG, a common side reaction that reduces monomer purity and degrades recycled polyester properties. Remarkably, the trace amount of PPOA-K remaining in the recovered BHET (17.3 ppm) is not detrimental; instead, it continues to inhibit DEG formation during repolymerization and acts as a thermal stabilizer, improving the melting point and thermal stability of recycled PET. The advantages of PPOA-K are further demonstrated in a partial (in situ) glycolysis–repolymerization process, where it reduces the DEG content in the final rPET to 1.78% (vs. 2.25% for conventional Zn(OAc)₂), yielding rPET with a higher melting point, higher crystallinity, and better color. This work demonstrates that dipotassium phenylphosphonate uniquely combines high catalytic activity, side reaction suppression, and beneficial residue effects, offering a new catalyst design strategy for high-quality PET recycling. Full article

Aquatic envenomations may cause severe tissue injury, neurologic morbidity, and even mortality among those whose leisure and/or occupational activities expose them to marine and freshwater animals. The Motoro ray, or Potamotrygon motoro (also known as an ocellate river stingray) is endemic to freshwater tributaries throughout Brazil, and is a frequent source of severe envenoming of local fisherman and those residing near waterways. Local wound management including immersion in warm water, wound cleaning and debridement, as well as antibiotics are mainstays of treatment, as are local anesthetics (e.g., nerve blocks) and systemic opioid analgesics; however, high-quality evidence supporting such interventions is lacking. We present a case of a Canadian who was envenomed by his pet Motoro ray, and describe his clinical presentation and evolution of symptoms over the subsequent months. With the ever-increasing trade of exotic wildlife, clinicians, public health authorities, and those within the broader wildlife regulatory ecosystem should be attuned for unanticipated adverse consequences, such as those described herein. We further situate this case within the existing published literature around this particular species of ray, which is not typically considered an ornamental fish. Full article

Alzheimer’s disease is a progressive neurodegenerative disorder in which early detection remains limited by the cost and invasiveness of positron emission tomography and cerebrospinal fluid testing. We evaluated whether plasma proteomic profiles could distinguish amyloid PET-positive from amyloid PET-negative individuals using the Bio-Hermes cohort. After quality control and missing-data filtering, 988 participants and 295 proteins were analysed; 31 proteins showing group differences were used for supervised classification. Random Forest, Gradient Boosting, and Neural Network models were trained across four train/test splits with repeated cross-validation and class downsampling. Amyloid-positive and amyloid-negative groups differed across a subset of proteins, with five remaining significant after false discovery rate correction. Tree-based models performed most consistently, with Random Forest and Gradient Boosting achieving AUC values of 0.79–0.81 and balanced accuracy of 0.68–0.73. Eight proteins (SERPINA1, C3, CRP, APOE4, CFH, VTN, C1QTNF5, and PON1) emerged as recurring high-importance features. These findings indicate that discovery-driven plasma proteomics can identify multi-protein signatures associated with amyloid status and can complement established single-analyte blood biomarkers by adding pathway-level information. Full article

Focusing on contrasting climate and land use pathways, this analysis explores the changing trajectories of water conservation function over time. An integrated framework combining the PLUS and InVEST models with Spearman’s correlation analysis and geographically weighted regression (GWR) was applied to examine the spatiotemporal heterogeneity and underlying drivers of water conservation function in the Chengdu–Chongqing Economic Zone during the period 2000–2020. Thus, it further predicted the evolution trend under two scenarios, namely SSP1-1.9 (Sustainable Development Pathway) and SSP2-4.5 (Medium Development Pathway), for the period 2030–2050. The findings reveal that: (1) Between 2000 and 2020, the spatial distribution of water conservation function shifted markedly, with low-value areas contracting and high-value zones expanding, alongside a progressive transition toward a predominantly medium-to-high functional structure. (2) In mountainous and hilly transition zones, precipitation (PRE) and forest cover proportion (FCP) exhibited notably positive effects, whereas evapotranspiration (PET) exerted a negative effect. In contrast, in plain and urbanized areas, built-up land proportion (BUP), population density (POP), and gross domestic product density (GDP) demonstrated pronounced negative effects. (3) Future simulations indicate that under the sustainable development pathway (SSP1-1.9), the combined area of high and extreme functional zones will recover by 2050, whereas under the moderate development pathway (SSP2-4.5), such extreme functional zones will be nearly eliminated. These results underscore the substantial impact of development pathways on regional water security and sustainability. Full article

Recent advancements in computer vision, particularly in detection, segmentation, and classification, have significantly impacted various domains. However, these advancements are still strongly tied to RGB-based systems, which are insufficient for applications in industries such as waste sorting, pharmaceuticals, and defence, where material characterization beyond shape or visible colour is necessary. Hyperspectral (HS) imaging captures spatial and spectral information for each pixel and therefore offers a promising route for material-level classification. This study evaluates the potential of combining HS imaging with deep learning for plastic material classification. The work includes: (i) the design of an experimental setup with a HS line-scan camera, conveyor, and controlled illumination; (ii) the construction of an object-disjoint dataset of HDPE, PET, PP, and PS samples with semi-automated mask generation and Raman spectroscopy-based labelling; and (iii) the development of P1CH, a lightweight pixel-wise 1D convolutional hyperspectral classifier. On object-disjoint test images, P1CH achieved 97.44% all-pixel accuracy. A boundary sensitivity analysis, reported separately because semi-automated labels are uncertain at material/background interfaces, yielded 99.94% accuracy after excluding a pre-defined two-pixel border band. Additional ablation, baseline, and robustness analyses show that the proposed pixel-wise spectral approach is effective for small fragments, visually similar plastics, and overlapping materials, while black or very dark plastics remain challenging under the present camera and illumination configuration. Full article

Background: Nutrition is a key determinant of skin barrier function and coat quality in cats, but controlled studies comparing commercial diets are limited. Methods: Eighteen healthy adult cats (3.44 ± 0.69 years) were randomly assigned to receive either a standard commercial chicken-based diet (ORI, Instinct Petfood, Visalia, CA, USA, n = 9) or a nutrient-enriched formula designed for skin and coat health (ULT, Instinct Petfood, Little Falls, MN, USA, n = 9) for 12 weeks. Body weight (BW) and body condition score (BCS) were recorded weekly. Visual coat scoring, quantitative descriptive analysis (QDA), and skin biophysical parameters (hydration, sebum, transepidermal water loss) were assessed on days 0, 21, 42, 63 and 84. Results: Both diets maintained stable BW and BCS. Visual scoring showed skin and hair remained within the normal healthy ranges in both groups throughout the study. QDA revealed significant improvements in coat glossiness, softness, and overall scores over time in both diet groups (p < 0.05). Back hydration increased over time, and in the ORI group, values at days 42, 63, and 84 were higher than baseline (44.96, 43.10, and 41.26 vs. 33.85; p < 0.05). Ear hydration showed a significant time × group interaction (p < 0.05). In the ULT group, ear hydration was higher at days 21, 42, 63, and 84 than at baseline (24.17, 35.21, 28.69, and 34.19 vs. 17.87; p < 0.05). Ear TEWL also showed a significant time × group interaction (p < 0.05) and displayed a biphasic pattern in the ULT group, with values at days 63 and 84 higher than at day 42 (15.51 and 14.48 vs. 11.77 g/m²/h; p < 0.05). Compared with the ORI group, the ULT group showed a smaller change in ear TEWL from baseline at day 42 (−2.54 vs. 2.98 g/m²/h; p < 0.05) and a greater increase in ear hydration from baseline at day 84 (16.32 vs. 9.31; p < 0.05). Conclusions: These findings indicate that both diets supported healthy skin and coat conditions in cats, while the nutrient-enriched formula may provide additional benefits for skin hydration. Full article

This retrospective quantitative data analysis study aimed to investigate sex- and age-related differences in the physiological distribution of [¹⁸F]FDOPA uptake in long axial field-of-view (LAFOV) PET images across a range of organs and tissues. A retrospective quantitative data analysis study of 106 anonymized PET/CT images acquired from vertex to mid-thigh with minimal abnormalities, divided in two gender groups and two age groups was used for this study. The mean and max lean body mass weighted standardized uptake values ($SU{L}_{mean}$, $SU{L}_{max}$), target-to-background ratios ($TBR$), and coefficients of variation ($CoV$) were used to quantify tracer uptake. Sex- and age-related differences in uptake were organ- and metric-specific. Most organs showed comparable uptake between males and females. However, males exhibited higher absolute uptake in metabolically active organs and females showed greater intra-organ heterogeneity. Aging was generally associated with increased tracer uptake and variability, especially in women, with the hip showing higher uptake in younger individuals. Statistically significant differences were most prominent in women and varied by organ and metric. In conclusion, both sex and age significantly influence [¹⁸F]FDOPA PET tracer uptake and variability in an organ- and metric-specific manner. Incorporating sex- and age-adjusted reference values may improve the accuracy and personalization of PET imaging in clinical and research settings. Full article