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  • Case Report
  • Open Access

Adverse Reactions to Yeast Beta-Glucan Supplementation in Two Dogs with Inflammatory Bowel Disease

  • Andressa Rodrigues Amaral,
  • Pedro Henrique Marchi and
  • Thiago Henrique Annibale Vendramini
  • + 5 authors

Beta-glucans are immunomodulatory compounds known to act as adjuvants in the beneficial regulation of various allergic conditions, particularly atopic dermatitis, as well as in the modulation of dysbiosis in patients with chronic gastrointestinal disorders. However, certain underlying conditions, such as inflammatory bowel disease (IBD) and the associated loss of oral tolerance, may alter the expected outcomes of supplementation and lead to dysregulated immune responses. This study reports cases of cutaneous allergic reactions in dogs with IBD following the oral administration of yeast-derived beta-glucan. Two dogs with controlled IBD received 9.13 ± 1.14 mg/kg of beta-glucan in a double-blind study designed to investigate its effects on the intestinal microbiota in IBD. Both animals were withdrawn from the study due to adverse effects, including cutaneous allergic manifestations and intense pruritus. These findings suggest that patients with impaired oral tolerance may develop cutaneous reactions following the ingestion of yeast-derived prebiotics.

1 February 2026

Animal 1 in the ventrodorsal position, showing papules, erythema, and pustules on the abdomen (a) and vulvar region (b).

Brachycephalic dogs commonly suffer from breathing difficulties and other chronic health problems, yet strong consumer demand is widely reported. Whether the popularity of brachycephalic breeds persists across different contexts is largely untested. Census data from 1,510,085 canine consultations conducted at a single veterinary hospital from 2004 to 2023 in Hong Kong were collected and analysed. Individual dogs (n = 96,777) were counted once, in the first year of presentation. Males (53.5% to 57.7%) and purebreds (86.2% to 91.2%) predominated each year. Neutered dogs increased from 52% to 70% annually. Poodles were the most popular breed from 2009. Brachycephalic breeds, collectively, declined progressively from 36.3% to 15.4% of the annual population. This trend was exaggerated in dogs < 1-year-old (27.4% to 11.7%). Absolute numbers of common brachycephalic breeds declined, except for French Bulldogs and Pugs. A historical cohort study was conducted with a subset of the data (2016–2023) to investigate the hypothesis that veterinary service use would be higher in brachycephalic breeds compared with other groups. The hypothesis was proven. Relative demand for brachycephalic breeds in Hong Kong is decreasing, with the exception of French Bulldogs and Pugs. Investigating drivers of these observations will inform behaviour change strategies, and facilitate comparisons across different regions.

1 February 2026

Annual trend in the proportion of dogs at first presention to a veterinary clinic in Hong Kong, 2004 to 2023, categorised by sex and neuter status.

Viper envenomation in dogs represents a significant medical emergency in regions where vipers are endemic. Despite its clinical relevance, detailed data on the haematological and biochemical alterations in canine viper envenomation remain limited. This study aimed to evaluate the clinical presentation and haematological, biochemical and coagulative changes occurring in dogs following bites from the Vipera aspis species, and to assess their diagnostic and prognostic significance. Twelve dogs with suspected Vipera aspis envenomation were encompassed in the study. Clinical data were gathered and blood samples were collected at hospital admission (T1), 24 h (T2) and 48 h later (T3). Complete blood counts, biochemical profiles and coagulation parameters were analysed using standard automated systems. Common clinical signs included local pain and swelling, depression, fever, haematuria and melena. Haematological evaluation revealed progressive anaemia, leucocytosis and thrombocytopenia. Biochemical findings showed elevated alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and creatine kinas (CK), indicating hepatic and muscular injury; however, no consistent evidence of renal failure was found. Coagulation analysis revealed a significant shortening of activated partial thromboplastin time (aPTT) and prothrombin time (PT) over time, alongside marked increases in fibrinogen and antithrombin III. This indicates an inflammatory rather than consumptive coagulopathy. Viper envenomation in dogs induces complex haematological and biochemical alterations, reflecting both direct venom toxicity and systemic inflammatory responses. Early recognition, supportive care and continuous laboratory monitoring are essential for improving prognosis.

20 January 2026

Blood smear of one of the dogs (ID1) included in the study. Venom within 24 h of the event. Sphero-echinocytes (erythrocytes lacking central pallor with a jagged outline and regular spicules along their entire circumference) (grey arrow). A neutrophil with a visible Döhle body inside (blue arrow). There is an almost total absence of platelets. Wright, 100× (immersion).
  • Case Report
  • Open Access

Protein-losing enteropathy (PLE) is a spectrum of gastrointestinal disorders in which protein loss occurs through the gastrointestinal tract. One of the underlying causes is chronic inflammatory enteropathy (CIE). Conventional therapies for CIE often include diet, immunosuppressives, anti-microbials, probiotics, and, recently, fecal microbial transplantation (FMT). This case report highlights the use of lyophilized material-based FMT through oral capsules and enema in a dog with PLE and concurrent protein-losing nephropathy (PLN). The patient initially had a significantly increased dysbiosis index (DI) and required repeated FMT treatments, resulting in a positive clinical response through improvement in body weight, serum albumin concentrations, fecal scores, and normalization of the DI over time. To maintain clinical responses, FMT had to be performed monthly. Approximately 1 year after starting FMT therapy, the patient then developed an episode of acute hemorrhagic diarrhea syndrome (AHDS) associated with netF-gene-encoding Clostridium perfringens strains, after which the DI became abnormal again. The patient responded clinically well to monthly FMT treatments again, but it took several months for normalization of the DI after the AHDS episode. In summary, this case report highlights the continued use of adjunct lyophilized FMT in a dog with PLE resulting in improved clinical control over time.

19 January 2026

Fecal dysbiosis and bacterial taxa. The dysbiosis index (A) and five bacterial taxa showing the greatest temporal variation (B–F) are displayed. Each sample was collected prior to a fecal microbiota transplantation (FMT) procedure. The patient experienced an episode of acute hemorrhagic diarrhea syndrome from 12 to 15 April 2024, indicated by the red shaded area. The gray shaded area represents the reference intervals. For P. hiranonis (B), the dashed line at log DNA 4 marks the bile acid conversion cutoff, with values below associated with abnormal conversion of primary to secondary unconjugated bile acids.

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Pets - ISSN 2813-9372