Pets

Bile acids (BAs) are important signaling molecules in the gastrointestinal (GI) tract and are associated with health and disease in humans and animals. Intestinal bacteria transform BA through deconjugation, dehydroxylation, and epimerization reactions, producing various isoforms, many of which have not been investigated in companion animal diseases. We aimed to develop and analytically validate a novel liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the quantification of 30 BAs in dog feces, with a simple extraction procedure and on-line solid-phase extraction. Validation demonstrated good accuracy, precision, sensitivity, spiking recovery, dilution, and stability for 29 BAs. The method was applied to fecal samples from healthy dogs (H; n = 121) and dogs with chronic enteropathy (CE; n = 58). The immediate and downstream products of bacterial 7α-dehydroxylation reactions with cholic acid were lower in concentration in dogs with CE when compared to healthy dogs (deoxycholic acid, 3-oxo-deoxycholic acid, and 12-oxo-lithocholic acid; q < 0.001). Across all fecal samples, the products of hydroxysteroid dehydrogenase (including oxo- and iso-BA) made up an average of 30% of the total measured fecal BA pool (glycine-BA, 0.1%; taurine-BA, 2.2%; unconjugated BA, 53%). Full article

Many stressors have been identified for shelter dogs, and while stress is a natural physical response, exposure to shelter stressors has been implicated in poor dog welfare including the formation of maladaptive behaviors that manifest as unappealing responses toward humans. Inappropriate behavioral reactions to humans lead to longer lengths of stay in the shelter, so identifying ways to reduce these responses to human presence would not only benefit the immediate welfare of the dog but could also decrease the amount of time the dog spends in the shelter. Previous studies have shown that response-independent food paired with an auditory stimulus reduced behaviors that likely prolong time to adoption; however, these interventions used an explicit discriminative stimulus where a more easily deployed design would be effective without requiring an additional stimulus to precede the food. The purpose of the present study was to extend that work to evaluate the effect of noncontingent reinforcement on reducing behavioral reactions to humans in the presence both of the experimenter and other people. Overall, the intervention was effective at decreasing human-directed behavioral responses but did not decrease the dogs’ length of stay. Changes in reactive behaviors appear to be limited to the presence of the experimenter during the first and last trials, with improvements generalizing to non-experimenters only for two behaviors: staying in the front half of the kennel and facing the kennel front. Our recommendation for shelters is that this intervention would be most effective at improving behaviors directed at a person delivering treats to the dog. Full article

Background: In veterinary medicine, fecal microbiota transplantation (FMT) shows promise for treating chronic enteropathy (CE) in dogs, but standardized protocols for dosage, preparation, and administration are lacking. This study aimed to evaluate the efficacy of freeze-dried FMT capsules (cFMT) and to investigate the existence of a possible optimal dosage for dogs with CE. Methods: A multicenter prospective study was conducted on 171 dogs with CE, treated with freeze-dried FMT capsules (100 mg for dogs ≤ 10 kg, 200 mg for dogs > 10 kg). The dosage of freeze-dried FMT material was expressed in different ways, to investigate the effect of putative active principles. Clinical outcomes were assessed by classifying dogs as responders (R) or non-responders (NR) based on veterinary evaluations from a questionnaire, along with changes in the CIBDAI score and variations in 15 clinical signs of chronic enteropathy (CE). Data were collected before and 15 days after treatment. Results: Of the 111 dogs included in the final analysis, 82% showed a positive clinical response, with no significant differences in clinical response between capsule sizes or dosage, irrespective of how it was expressed. Conclusion: Effective dosage range for cFMT administration in dogs affected by CE was defined. The oral administration of 100 mg of freeze-dried cFMT daily for a month was shown to be sufficient to achieve an 80% response rate. Further studies are needed to explore additional factors that may influence the overall effectiveness of cFMT in treating CE. Full article

In recent years, the European companion animal veterinary care market has experienced significant growth, with 166 million households (50%) owning one or more of Europe’s 352 million companion animals. This expansion is accompanied by a notable market trend toward the corporatisation of veterinary practices, particularly in urban areas and certain countries. Corporatisation has reshaped the profession, offering benefits such as economies of scale, advanced veterinary treatments, and structured career pathways for early-career veterinarians. However, this shift also prompted discussions on the impact it has on rising costs of care, professional autonomy constraints, and potential reductions in rural practice attractiveness. Our comprehensive study, combining a large-scale survey of European veterinarians, semi-structured questionnaires, and an extensive literature review, maps the current state of corporatisation in Europe, identifies key players, and examines its impact on the veterinary profession and broader society. The findings underscore the rapid expansion of corporate veterinary practices and the accompanying competitive pressure on independent vet-owned clinics. To ensure a sustainable future for the profession, our research highlights the need for further investigation to fully comprehend the effects of corporatisation. Addressing these challenges is essential to protect the interests of animals and their caretakers, veterinarians, and the wider society. Full article

Plant extracts, such as olive extract (OE), have been used in human and pet foods for their biological benefits; however, no available data have demonstrated OE’s effect on palatability in dogs. The current study aimed to evaluate acceptance of dry and canned dog foods with differing inclusions of OE as a flavor component. Flavor compounds in OE were analyzed by gas chromatography–mass spectrometry and high-pressure liquid chromatography, detecting 137 volatile compounds, including acetic acid and hydroxytyrosol. Dog kibbles were coated with liquid commercial palatants containing OE that resulted in application rates of 0 (control), 120, 200, and 500 ppm of OE in the diets. OE was also added at 0 (control), 120, 200, and 500 ppm into a wet food formulation with a commercial palatant before retort processing. Two separate panels of adult beagles were used for monadic testing to determine acceptance rates for kibble (5/treatment; 20 total) and canned foods (4/treatment; 16 total) in a 4 × 4 Latin square design. None of the tested inclusions impacted food acceptance in this preliminary study (p > 0.05). As a flavor ingredient, OE can be added into dry or wet dog food up to 500 ppm without deterring effects on palatability. Full article

Olive extract (OE) has been used in human foods for its nutraceutical effects, making it a product of interest for pet food. However, OE’s effect on palatability has not been examined. The study objective was to evaluate the palatability of dry cat foods with OE applied at differing inclusions within liquid or dry palatants. Twenty-seven volatile compounds were identified by gas chromatography–mass spectrometry for a potentially earthy or fruit-like flavor profile. Liquid palatants were formulated to supply 0 (control), 15, 30, 50, 75, and 150 ppm OE, and dry palatants were formulated to provide 0, 100, 200, 400, and 600 ppm OE when coated onto kibble. Palatability was evaluated using two-day, two-bowl testing of OE-containing versus control rations in adult cats (n = 20) with two-tailed t-tests to determine if OE affected intake ratio (IR). The observed IR of rations with OE were 0.45 to 0.56. The only preference was the 200 ppm treatment (IR = 0.56; p = 0.01) while the other OE rations were not different from the control (p ≥ 0.05). These findings indicate that palatant formulations can supply kibble diets with up to 150 ppm OE for liquid and 600 ppm for dry applications without negatively impacting cat food palatability. Full article

Children with psychosocial, developmental or physical impairments benefit from equine-assisted therapy (EAT) in multiple ways. However, to date, the animal perspective of such interventions has received comparatively less scientific dedication. Thus, heart rate (HR) and heart rate variability (HRV) of seven therapy horses that lived in an open stable environment and participated in therapeutic group sessions with children were monitored within 60 min prior to and within 90 min after EAT. Moreover, cardiovascular activity was compared to a control condition on a day without any EAT sessions. No significant differences in HR or HRV were found, neither before nor after EAT nor under the control condition. These findings do not give rise to any concern regarding horse welfare in the studied setting but cannot be generalized to a broader population of equines serving in EAT. Given the enormous heterogeneity in EAT, more in-depth research is warranted using behavioral and physiological indices of equine health and wellbeing. Full article

Human–animal relationships have long been idealized as universally positive, but recent studies highlight the complexities and contradictions inherent in this bond. This study re-analyzes previously published data to examine the nuanced effects of pet ownership on human well-being, particularly under stressful circumstances such as the COVID-19 pandemic. Using a mixed-methods approach, data from three independent surveys (n = 840) were re-analyzed to explore the bio-psychosocial implications of pet companionship. Key instruments included the Quality of Life Questionnaire (WHOQOL-BREF), Beck Depression Inventory (BDI-II), and Lexington Attachment to Pets Scale (LAPS). Results revealed that pet ownership was significantly associated with higher levels of psychosocial stress, challenging the assumption that pets universally improve quality of life. Notably, individuals with current pet ownership reported lower quality of life compared to non-owners. However, qualitative analyses illustrated perceived emotional and social support among pet owners, reflecting the “pet-effect paradox.” Mental health variables such as depression and loneliness emerged as stronger predictors of well-being than pet attachment. This study introduces the “Aunty Phenomenon” as a conceptual framework to describe the idealization of pet relationships, while acknowledging the responsibilities they entail. These findings underscore the importance of a holistic approach to human–animal bond research. Full article

Background: The growth of the older adult population calls for innovative and cost-effective ways of promoting their physical, psychological, and cognitive health. Human–animal interaction, including pet ownership, is related to positive and negative aspects of human health. Not all pet owners respond in the same way. The levels of pet attachment and pets’ influence on their owners’ lives could moderate the relationship between psychological status and health outcomes. Purpose: We examined the moderating role of pet attachment in the relationships of psychological status (mental wellbeing, happiness, anxiety, depression) to physical function (physical wellbeing, usual- and rapid-gait speeds, physical performance battery) in community-residing older adult pet owners. Methods: A cross-sectional, secondary analysis of pet-owning older adult participants in the Baltimore Longitudinal Study of Aging (n = 178). Results: In regression analyses, controlling for age, gender, and comorbidities, pet attachment and pet influence moderated the relationships of physical wellbeing to mental wellbeing and anxiety (p < 0.05). Pet influence also moderated the relationship between anxiety and usual gait speed (p < 0.05). Conclusions: Greater attachment and influence buffer the relationship of perceptions of poor mental function with perceptions of poor physical wellbeing suggesting one mechanism for health benefits of human-animal interaction. Full article

Several recent studies have reported a significantly greater prevalence of Clostridium perfringens encoding the novel pore-forming netF toxin gene in dogs with acute hemorrhagic diarrhea syndrome. However, the presence of netF in other canine diarrheal diseases remains poorly characterized. This retrospective, cross-sectional study aimed to describe the prevalence and abundance of netF-positive C. perfringens in fecal samples from 352 dogs with acute and chronic gastrointestinal diseases. Dogs were divided into five groups: acute hemorrhagic diarrhea syndrome (AHDS), acute diarrhea (AD), chronic enteropathy (CE), exocrine pancreatic insufficiency (EPI), and healthy controls (HCs). The abundances of C. perfringens 16S rRNA, the C. perfringens enterotoxin gene and the C. perfringens netF gene in fecal samples were analyzed by quantitative polymerase chain reaction. In total, 7 of 15 (46.7%) dogs with AHDS, 10 of 75 (13.3%) dogs with AD, 2 of 120 (1.7%) dogs with CE, 1 of 12 (8.3%) dogs with EPI, and 1 of 130 (0.8%) HC dogs tested positive for netF. This study provides further evidence that NetF may be a significant contributor to the etiology of AHDS and potentially to a subset of acute nonhemorrhagic diarrhea cases, while it was only rarely detected in chronic gastrointestinal disease phenotypes. Full article

(1) Each year, thousands of students leave their pets behind to attend university, often causing separation anxiety and losing a vital stress-coping resource. While many universities offer animal visitation programs (AVPs), their effectiveness in supporting student wellbeing during this transition remains unclear. This randomized controlled trial evaluated psychological mood risk and resilience in a randomly selected sample of first-year university students (n = 145) separated from their childhood pets. (2) Participants were randomly assigned to receive access to a seven-session, biweekly 2 h drop-in program (n = 77) featuring unstructured interactions with therapy dogs or a waitlist control group (n = 68). Assessments of wellbeing were conducted at the start, middle, and end of the semester including depression, anxiety, worry, stress, cognitive reappraisal, expressive suppression, and self-compassion. (3) Regression analyses showed that access to the semester-long drop-in program significantly flattened trajectories of depression (B = −3.05, p = 0.01, d = 0.514), worry (B = −3.92, p = 0.04, d = 0.416), and stress (B = −1.94, p = 0.05, d = 0.386) compared to the control group. Students in experimental conditions also showed improvements in self-compassion (B = 4.03, p < 0.001, d = 0.605). (4) These findings suggest regular access to unstructured drop-in programs featuring therapy dogs may provide valuable psychological support for students adjusting to university life. Full article

Toxoplasma gondii is a protozoan parasite in cats that can be a cause of zoonotic concern due to sheding infectious and highly resistant oocysts. Clinical toxoplasmosis is rare in cats and can occur with mild to severe clinical signs or even fatal outcomes. T. gondii immunoglobulin M (IgM) or IgG is an indicator of ongoing or prior infection. Information about T. gondii occurrence in the domestic cat population, primary clinical concerns about affected cats, and potential risk factors is scarce in Germany. This retrospective study explored data from 174 feline patients (≥12 weeks old) presented to a tertiary veterinary care facility in central Germany between 2012 and 2023. T. gondii serological status, determined as part of the cats’ routine diagnostic evaluation, was assessed in relation to patient characteristics, environmental factors, medical history data, and clinical diagnosis. Neurological (57%), locomotor (25%), and ocular signs (11%) were common, and older age was associated with the presence of IgG (p = 0.008). Sex had no significant effects on T. gondii antibody positivity (p = 0.865). More outdoor cats than indoor-only cats were T. gondii IgG-positive (p = 0.021), and IgG-seropositive animals received a canned/wet diet more often than a dry kibble diet (p < 0.0001). Clinical representations aligned with previous studies, and age, choice of food, and outdoor living (p = 0.021) were significant predisposing factors. Full article

Animal shelters play a pivotal role in protecting pets in crisis and bringing families together. This paper adds to the work of Hoy-Gerlach and colleagues which outlined a three-tier framework for developing and training veterinary social workers (VSWs) to serve in animal shelters. Our research questions sought to explore the VSWs’ self-identified role (RQ1), and how VSWs self-identify their scope of practice (RQ2). Through these exploratory questions, our research aimed to advance knowledge about the state of VSWs in the animal-shelter setting. Methods: Using a purposive sampling strategy, an anonymous community practice survey (CPS) was emailed to VSWs working in animal shelters. Inclusion criteria included being over the age of 18, being currently employed in an animal shelter, and cognitive fluency in English. The survey yielded 25 responses with 14 included in the data analysis. Findings: The VSWs identified their role as supporting multiple groups, including clients and their practice community (i.e., colleagues). While most of their time was devoted to individuals from the community, they also supported the mental wellbeing of their team. Their work ranged from case management and psychoeducation to crisis intervention and suicide risk assessment. As there are no standards of practice for VSWs, each respondent used unique language to explain their role and scope of practice. Conclusions: The CPS was helpful in illuminating developing trends within the field, though a larger sample size is needed to gain a more nuanced understanding of self-identified needs of VSWs practicing in animal shelters and other related spaces. Full article

Service dogs (SDs) have been shown to provide multi-faceted benefits for autistic children, with some benefits also extending to family members. The main objectives of this study were to explore the relationships between psychosocial well-being, family functioning, parent anxiety, and parent–child relationships in families of autistic children, and to investigate the effects of integration of an SD. A cross-sectional approach was used to collect data from three groups of parents of autistic children: (1) families approved for an SD but waitlisted, (2) families in the training process but not yet paired with an SD, and (3) families with a child matched with an SD. Parents completed an online questionnaire including validated tools. Children’s autism-related symptoms correlated with different aspects of family experience and quality of life, and parents’ anxiety scores were related to children’s social anxiety and difficulties associated with parenting an autistic child. Family functioning was associated with children’s autistic characteristics, parents’ anxiety, and the quality of the parent–child relationship. In families of children paired with an SD, children’s autistic symptoms were related to parental attachment with the SD. These findings underscore the need for further research on the assessment and implications of autism-related symptoms for children acquiring an SD. Full article

Background: The Dutch branch organization for pet products promised the public that it will improve the quality of raw meat-based diets (RMBDs) for dogs after several diagnoses of tuberculosis, brucellosis, and hyperthyroidism in dogs fed RMBDs. Objective: The objective of this study was to re-evaluate the risk factors of commercially available raw meat diets for dogs in The Netherlands. Methods: Seven commercial brands of RMBDs that were previously investigated were re-tested, as well as a newly introduced high-pressure processing (HPP) product. Raw beef sausage for humans was included for comparison. In total, 40 animal RMBDs (five batches per product) were tested for the presence of colony-forming units (CFUs), Salmonella spp., and Escherichia coli directly after defrosting and 4 h later, as well as thyroid hormone. Results: Exceeded EU standards for CFUs and Salmonella bacteria were present in several samples. In the HPP product, bacteria were still present; however, the counts were lower. There were no differences in CFUs directly after defrosting and 4 h later. The human raw meat product was negative for bacteria. Thyroid hormone could be detected in 20 out of 37 samples. In seven of these samples, the levels were >0.75 µg/g, which have been associated with hyperthyroidism. Conclusions: The hygiene (including the use of HPP production) and accurate removal of thyroid tissue during the production of RMBDs still need attention to prevent the presence of zoonotic bacteria, high CFUs, and diet-induced hyperthyroidism. Full article

The purpose of this study was to assess the practical implications of supplementing soluble fiber in the diet of dogs. Dogs with a history of managed or active chronic enteropathy were randomized to receive either wheat dextrin (fiber group) or maltodextrin (placebo group) mixed with food once daily for 28 days. Owners recorded a daily fecal score one week prior to and during the supplementation period. Shallow shotgun sequencing, quantitative PCR abundances of core bacterial taxa, and short-chain fatty acid (SCFA) concentrations via gas chromatography/mass spectrometry were performed on fecal samples collected before and after supplementation. Seventeen dogs completed the study (fiber group: nine dogs; placebo group: eight dogs). The change in fecal score differed between groups, with the fiber group developing softer stools (p = 0.03). Alpha diversity, quantified PCR abundances of the SCFA-producing taxa, and fecal SCFA concentrations were not different after supplementation in either group. Fecal microbial communities differed between baseline and day 28 for fiber and placebo groups (p = 0.02, respectively); however, the size effect (ANOSIM R = 0.18 and R = 0.26, respectively) was minimal. In this small group of dogs fed variable commercial diets, the additional intake of wheat dextrin powder supplement was well accepted, but had minimal discernable clinical benefit, and could soften stools. Full article

Despite an increase in research into cat and dog cognition, behavior, and welfare in recent decades, it is unclear whether pet owners are aware of recent advances in our understanding of cats and dogs. Misunderstandings about the reasons for animal behavior can lead to negative welfare outcomes for the animal, and potentially distress for the owner, so it is important for owners to understand the latest science on animal behavior. Current and former pet owners (N = 224) completed an online survey rating their agreement with a series of statements about cat and dog behavior. After completing the survey, participants were provided with a document describing the latest scientific knowledge about each of the statements in the survey. For both species, participant beliefs generally accord with the latest scientific knowledge, but there is evidence of remaining beliefs about aspects of dominance training theory in dogs, and the belief that cats are low-maintenance pets. These findings can be used by trainers and behaviorists to help educate owners about pet needs, working from the owner’s baseline knowledge and debunking persistent myths. Full article

This study provides an overview of the new Greek legislative framework and highlights its progressive nature in tackling one of the most populous stray animal populations in the world. We present the newly established law and discuss its provisions, aims, and challenges. We also examine the reception of the law by key stakeholders such as veterinary practitioners, animal welfare organisations, and the Hellenic Kennel Club. With the post-implementation review scheduled for 2026, its evaluation has yet to be conducted. Full article

Adult-onset spontaneous feline hypothyroidism (SH) is considered rare, but its prevalence is unknown. This study aimed to screen laboratory submissions for cats with laboratory suspected SH (LSSH) and to identify laboratory abnormalities associated with LSSH. Submissions to a commercial laboratory in Germany between January 2022 and April 2023 were prospectively screened for cats aged 3–12 years with low total thyroxine (TT4); in these cats, thyrotropin (TSH) was measured. Iatrogenic hypothyroidism was excluded by contacting submitting veterinarians. Creatinine, triglyceride and cholesterol concentration and red blood cell count (RBC) of cats with LSSH (low TT4, TSH > 0.53 ng/mL; i.e., [one sided TSH reference change value (76%) of TSH upper reference interval (RI)] + [TSH upper RI] = 0.53) were compared to euthyroid cats (TT4 within RI) and cats with suspected non-thyroidal illness (SNTIS) (low TT4, normal TSH (<0.3 ng/mL; upper RI)) by non-parametric tests. p < 0.05 was significant. In total, 31,572 submissions of cats were included, 25,169 (79.7%) were euthyroid, 3818 (12.1%) had SNTIS and 61 (0.2%) had LSSH. Cats with LSSH had higher creatinine (p = 0.002) and lower RBC count (p < 0.0001) than euthyroid cats as well as higher creatinine (p = 0.00035) than cats with SNTIS. Azotaemia (creatinine > 140 µmol/L) and anaemia (RBC < 7.2 × 10¹²/L) were present in 28/49 (57%) and 19/44 (43%) cats with LSSH, respectively. There was no difference between LSSH and SNTIS for the remaining parameters. In cats with low TT4, azotaemia and anaemia might indicate the presence of SH and reinforce the need for TSH testing. Hypercholesterolaemia and hypertriglyceridaemia are not indicators of SH. Full article