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Probiotics, prebiotics, and postbiotics are of interest for their potential gastrointestinal and immunological benefits in pet health. This study aimed to assess whether a unique blend of Bacillus subtilis, Bacillus clausii, Bacillus coagulans (Weizmannia coagulans), FOS, GOS, and a postbiotic yeast extract could provide beneficial gut and immunological effects when fed to healthy, adult dogs. Twenty-four healthy adult beagle dogs (mean age 5.17 yrs) were fed the probiotic, prebiotic, and yeast chew (PPYC) or control chew (CC) supplement for 31 days, accompanied by fecal and blood sampling. Following 31 days, PPYC fed dogs had decreased (p < 0.05) fecal calprotectin concentration, a biomarker indicative of reduced intestinal inflammation, compared with dogs receiving the CC. In the PPYC group, blood C-reactive protein levels, an indicator of tissue inflammation, tended (p = 0.11) to be reduced. In addition, dogs receiving the PPYC supplement showed an increase in the IL-17a cytokine (p < 0.05). Despite dogs being in a clinically healthy state, changes in some dysbiosis-related bacterial strains were observed. There was an increase (p < 0.05) in the % of total bacteria of Blautia in the PPYC group by the end of the study, as well as an increase in the percent change from Day 0 of C. hiranosis (p < 0.05). Increased alpha diversity, a measure related to the resilience to environmental change, was observed in the PPYC group (p < 0.05). These results suggest that after consuming a supplement containing probiotics, prebiotics and a postbiotic yeast extract, markers of gut and systemic health were improved in otherwise healthy dogs.

4 January 2026

Mean food consumption as expressed per unit of body weight held constant for PPYC but increased for CC. Note: There was a significant difference between the slopes (p &lt; 0.01) but not at time 0 (p &gt; 0.20). Further, the slope of CC was significantly different (p &lt; 0.05) from Time 0 but not for PPYC.
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DEA 1 Prevalence and Theoretical Transfusion Mismatch Estimates in Dogs from Southeastern Mexico

  • Carlos Antonio Arcos-Roa,
  • Luis Arturo Ortíz-Carbajal and
  • Jose Luis Bravo-Ramos
  • + 6 authors

Ensuring blood group compatibility is essential in canine transfusion medicine, and Dog Erythrocyte Antigen (DEA) 1 is the most clinically relevant erythrocyte antigen due to its immunogenicity. However, information on its prevalence in Mexico is scarce. This study assessed the distribution of DEA 1 in client-owned dogs from Veracruz–Boca del Río, southeastern Mexico, and applied antigen frequencies to generate theoretical incompatibility scenarios in untyped populations. A cross-sectional study was conducted using blood samples from 192 clinically healthy dogs. DEA 1 typing was performed using a commercial immunochromatographicassay. Frequencies were compared using chi-square or Fisher’s exact test, and 95% confidence intervals were calculated with the Wilson method. Of all dogs sampled, 60.9% (95% CI: 53.6–67.8) were DEA 1-positive. No statistically significant differences were observed by sex or municipality (p > 0.05). Although frequencies varied among breeds, no significant association between breed and DEA 1 status was detected (p > 0.05). Based on observed antigen proportions, mathematical estimations were calculated to illustrate how DEA 1 distribution alone may contribute to potential donor–recipient mismatching when typing is not performed. These estimations are theoretical values and do not represent clinically documented sensitization or transfusion reactions. This study provides the first report of DEA 1 distribution in southeastern Mexico and establishes baseline information that may support future research on canine transfusion practices and donor selection.

18 December 2025

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In recent years, requests for animal-assisted interventions (AAI) from medical institutions and welfare facilities have increased. Dogs are the most commonly used animals in AAI. Dogs that pass the “therapy dog” aptitude test can work in AAI. In previous research, we identified the Canine Behavior Assessment and Research Questionnaire (C-BARQ) factors common among dogs that passed the aptitude test. Using these factors, we developed the TC-BARQ, a screening questionnaire for therapy dogs that included 51 C-BARQ surveys. In this study, we conducted TC-BARQ screenings and compared the characteristics of dogs that passed and failed the aptitude test. We collected TC-BARQ data points from aptitude test examinees of the local AAI Dog Association. Each dog is identified by its breed, sex, neutering status, and whether it lives with another dog at home. For each question, we identified factors that differed between dogs that passed and those that failed. As a result, differences emerged in the presence of family dogs, particularly in behaviors related to aggression toward strangers and other dogs, as well as excitability toward people and situations. Continued surveillance is essential, but this study provides important information on selecting “therapy dogs”.

6 December 2025

Family dog presence and pass rate. Passed dogs: with family dogs n = 42 (61%); without family dogs n = 27 (39%); Failed dogs: with family dogs n = 11 (35%); without family dogs n = 20 (65%).

Myocutaneous Flaps and Muscle Flaps for Management of Limbs’ Defects in Dogs and Cats: A Review

  • Mandalena Markou,
  • Eleftheria Dermisiadou and
  • Konstantina Karagianni
  • + 2 authors

The objective of the present study is to review the anatomical considerations, surgical techniques, clinical applications, and outcomes of myocutaneous and muscle flaps used in the reconstruction of limb defects in dogs and cats. Limb wounds in small animals often result from trauma, neoplasia, or infection and can involve significant soft tissue loss. Reconstruction of these defects is challenging due to limited local skin availability, particularly in distal regions, and the need to preserve function while preventing complications. Muscle and myocutaneous flaps provide well-vascularized tissue suitable for covering complex wounds, especially those with exposed bone, joints, or tendons. This review synthesizes current literature on commonly used flaps—including latissimus dorsi, cutaneous trunci, trapezius, sartorius, semitendinosus, and flexor carpi ulnaris; focusing on their anatomical basis, vascular supply, arc of rotation, surgical technique, indications, and complication rates. Comparative data between dogs and cats are highlighted, and experimental as well as clinical applications are discussed. Myocutaneous flaps offer durable and reliable coverage with lower infection and necrosis rates compared to skin grafts, particularly in contaminated or poorly vascularized wounds. Common complications include distal flap necrosis, wound dehiscence, seroma, and, occasionally, functional deficits. Muscle and myocutaneous flaps remain essential tools in limb reconstruction. Successful outcomes require careful flap planning, surgical expertise, and vigilant postoperative care. Further prospective studies are needed to optimize flap selection and reduce complication rates in both species.

5 December 2025

Anatomic landmarks of the latissimus dorsi myocutaneous; including the ventral border of the acromion, the caudal border of the triceps muscle, the head of the last rib, and the distal third of the humerus and arc of rotation to the forelimb.

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Pets - ISSN 2813-9372