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890 Results Found

  • Article
  • Open Access
7 Citations
1,713 Views
13 Pages

23 February 2025

Quercus robur L., also referred to as “summer oak” or “English oak”, is an esthetically pleasing species, making it an excellent choice for street trees and gardens. Raising Quercus presents several challenges, including its l...

  • Communication
  • Open Access
4 Citations
3,901 Views
10 Pages

The current study investigated the impacts of light quality and different levels of fertility on mineral nutrient concentrations in the shoot and root tissues of Chinese kale (Brassica oleracea var. alboglabra). “Green Lance” Chinese kale was grown u...

  • Article
  • Open Access
28 Citations
6,123 Views
16 Pages

Enhancing the competence for plant regeneration in tissue culture studies is an important issue not only for efficient genetic transformation of commercial crops but also for the reproducibility of scientific reports. In this study, we investigated o...

  • Article
  • Open Access
2 Citations
1,348 Views
12 Pages

The Establishment of a Highly Efficient In Vitro Regeneration System for Viburnum opulus L. ‘Roseum’

  • Yajing Ning,
  • Hao Dong,
  • Xinxin Zhang,
  • Yanhua Li,
  • Chengpeng Cui and
  • Shujuan Li

26 January 2025

Viburnum opulus L. ‘Roseum’ is a highly valuable ornamental plant for landscaping, but it has a long propagation cycle and low propagation coefficient. In this study, stem segments with axillary buds from Viburnum opulus L. ‘Roseum&...

  • Communication
  • Open Access
18 Citations
4,600 Views
9 Pages

4 July 2019

The Arabidopsis high-affinity K+ transporter (AtHKT1;1) plays roles in salt tolerance by unloading Na+ from the root xylem to the xylem parenchyma cells and/or uploading Na+ from the shoot/leaf xylem to the xylem parenchyma cells. To use this promote...

  • Article
  • Open Access
17 Citations
3,369 Views
19 Pages

Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development

  • Nitsan Lugassi,
  • Ofer Stein,
  • Aiman Egbaria,
  • Eduard Belausov,
  • Hanita Zemach,
  • Tal Arad,
  • David Granot and
  • Nir Carmi

11 April 2022

Sucrose synthase (SuSy) and fructokinase (FRK) work together to control carbohydrate flux in sink tissues. SuSy cleaves sucrose into fructose and UDP-glucose; whereas FRK phosphorylates fructose. Previous results have shown that suppression of the SU...

  • Article
  • Open Access
3 Citations
2,246 Views
20 Pages

14 January 2025

Haworthia truncata, a species native to South Africa, is characterized by its limited growth and scarcity, contributing to high production costs. Countries like China and Turkey are known for exporting Haworthia globally. Tissue culture offers an eff...

  • Article
  • Open Access
3 Citations
7,310 Views
22 Pages

10 September 2024

Producing uniform Cannabis sativa (Cannabis) for medicinal/recreational flower production through sexual propagation has been problematic, leading to dominance of clonal propagation from “mother plants” in the cannabinoid industry, which...

  • Article
  • Open Access
6 Citations
2,149 Views
14 Pages

Shoot Organogenesis and Regeneration from Leaf Seedlings of Diospyros oleifera Cheng

  • Yang Liu,
  • Naifu Zhou,
  • Chengrui Luo,
  • Qi Zhang,
  • Peng Sun,
  • Jianmin Fu,
  • Shuzhan Li and
  • Ze Li

9 October 2023

Persimmons (Diospyros) are economically important trees that are widely cultivated for wood production in China, and Diospyros oleifera Cheng is the main persimmon grafting stock. However, an efficient tissue culture system has not been perfected for...

  • Article
  • Open Access
2,108 Views
14 Pages

An Efficient and Rapid Protocol for Somatic Shoot Organogenesis from Juvenile Hypocotyl-Derived Callus of Castor Bean cv. Zanzibar Green

  • Danaya V. Demidenko,
  • Nataliya V. Varlamova,
  • Taisiya M. Soboleva,
  • Aleksandra V. Shitikova and
  • Marat R. Khaliluev

Aseptic seedlings of different ages derived from surface-sterilized mature seeds were applied as an explant source. Various explants such as 7- and 21-day-old hypocotyl fragments, 42-day-old nodal stem segments, and transverse nodal segments of stem,...

  • Article
  • Open Access
3 Citations
2,339 Views
15 Pages

Network of miR396-mRNA in Tissue Differentiation in Moso Bamboo (Phyllostachys edulis)

  • Ying Li,
  • Naresh Vasupalli,
  • Ou Cai,
  • Xiaofang Lin and
  • Hongyu Wu

1 March 2023

MiR396 plays an essential role in various developmental processes. However, the miR396-mRNA molecular network in bamboo vascular tissue differentiation during primary thickening has not been elucidated. Here, we revealed that three of the five member...

  • Article
  • Open Access
564 Views
24 Pages

27 October 2025

This paper presents an analysis of the phenomena that occur during photodynamic therapy (PDT). For this purpose, models of laser energy deposition, bioheat transfer, and reactions occurring during the PDT process were used. Light distribution was est...

  • Article
  • Open Access
21 Citations
4,453 Views
23 Pages

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

  • O. New Lee,
  • Gunes Ak,
  • Gokhan Zengin,
  • Zoltán Cziáky,
  • József Jekő,
  • Kannan R.R. Rengasamy,
  • Han Yong Park,
  • Doo Hwan Kim and
  • Iyyakkannu Sivanesan

26 October 2020

This study aimed to investigate the impact of plant growth regulators, sucrose concentration, and the number of subcultures on axillary shoot multiplication, in vitro flowering, and somaclonal variation and to assess the phytochemical composition, an...

  • Article
  • Open Access
780 Views
15 Pages

Generation of state-of-the-art highly productive cabbage genotypes (Brassica oleracea convar. capitata (L.) Alef.) with improved agronomic traits is attainable using modern biotechnological approaches. However, capitata cabbage is relatively recalcit...

  • Article
  • Open Access
379 Views
13 Pages

Establishment of an In Vitro Regeneration System Using Shoot Tips of Iris setosa

  • Ruoqi Liu,
  • Siyu Lu,
  • Ying Qian,
  • Yuan Meng,
  • Yunwei Zhou,
  • Xue Yang and
  • Yun Bai

Iris setosa is a characteristic perennial wild herbaceous flower in the Changbai Mountain region of China, boasting significant ornamental and medicinal values. Given the increasing scarcity of its wild resources, this study developed an efficient in...

  • Article
  • Open Access
26 Citations
4,936 Views
13 Pages

Zinc (as an essential phytonutrient) and cadmium (as a toxic but readily bioavailable nonessential metal for plants) share similar routes for crossing plant biomembranes, although with a substantially different potential for translocation into above-...

  • Review
  • Open Access
62 Citations
6,305 Views
19 Pages

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

  • Min-Rui Wang,
  • Wenlu Bi,
  • Mukund R. Shukla,
  • Li Ren,
  • Zhibo Hamborg,
  • Dag-Ragnar Blystad,
  • Praveen K. Saxena and
  • Qiao-Chun Wang

13 September 2021

Cryopreservation is considered an ideal strategy for the long-term preservation of plant genetic resources. Significant progress was achieved over the past several decades, resulting in the successful cryopreservation of the genetic resources of dive...

  • Article
  • Open Access
4 Citations
1,840 Views
16 Pages

19 March 2024

Plants can utilize different N forms, including organic and inorganic N resources, and show great differences in the utilization efficiency of each N form among species and genotypes within a species. Previously, we found that the Tibetan wild barley...

  • Article
  • Open Access
1 Citations
1,691 Views
11 Pages

Tissue Culture Response and In Vitro Plant Regeneration of Malus ‘Baiyun’ (a New Cultivar of Ornamental Crabapple)

  • Jingze Ma,
  • Junjun Fan,
  • Wangxiang Zhang,
  • Ruomiao Zhou,
  • Yiting Shen,
  • Qin Peng,
  • Huimin Li and
  • Cong Lei

26 July 2024

Malus ‘Baiyun’ (registration no. 20210210), a new crabapple cultivar, was registered in 2021 by the Nanjing Forestry Unversity. However, the difficult rooting has greatly limited the production of high-quality M. ‘Baiyun’ in i...

  • Article
  • Open Access
5 Citations
3,718 Views
16 Pages

11 February 2023

Persimmon (Diospyros) is an economically important tree widely cultivated for woody grain production in China, and Diospyros lotus is mainly used as the grafting stock of persimmon. However, the breeding of stress-resistant rootstocks of D. lotus usi...

  • Essay
  • Open Access
6 Citations
2,978 Views
11 Pages

Rhododendron decorum is a woody species with high ornamental and medical value. Herein, we introduce a novel in vitro regeneration method for R. decorum. We used flower buds to develop an efficient and rapid plant regeneration protocol. Sterile flowe...

  • Technical Note
  • Open Access
20 Citations
5,172 Views
10 Pages

A New Clonal Propagation Protocol Develops Quality Root Systems in Chestnut

  • Patrícia Fernandes,
  • Sara Tedesco,
  • Inês Vieira da Silva,
  • Carmen Santos,
  • Helena Machado and
  • Rita Lourenço Costa

29 July 2020

There is, at the present time, a great demand for chestnut rootstocks with improved resistance to Phytophthora cinnamomi Rands in the nurseries. New genotypes are emerging from European chestnut breeding programs and the production of thriving plants...

  • Article
  • Open Access
5 Citations
5,047 Views
15 Pages

Phytochemical Profiling, Antioxidant and Tyrosinase Regulatory Activities of Extracts from Herb, Leaf and In Vitro Culture of Achillea millefolium (Yarrow)

  • Karolina Czech,
  • Katarzyna Gaweł-Bęben,
  • Agnieszka Szopa,
  • Wirginia Kukula-Koch,
  • Thomas Jakschitz,
  • Günther Bonn,
  • Shah Hussain,
  • Paweł Kubica,
  • Halina Ekiert and
  • Kazimierz Głowniak

15 June 2023

Achillea millefolium L. is one of the most known medicinal plants with a broad spectrum of applications in the treatment of inflammation, pain, microbial infections and gastrointestinal disorders. In recent years, the extracts from A. millefolium hav...

  • Feature Paper
  • Article
  • Open Access
5 Citations
2,604 Views
16 Pages

Studies on Seed Germination and Micropropagation of Ebenus sibthorpii, an Endemic Shrub of Greece with Potential Ornamental Use

  • Konstantinos Bertsouklis,
  • Dimitra Vazaka-Vodena,
  • Apostolos-Emmanouil Bazanis and
  • Maria Papafotiou

The introduction of new ornamental species is a challenge for the floriculture industry. Ebenus sibthorpii is an endemic species of Greece, with a strong ornamental potential. To the best of our knowledge, there are no studies on the in vitro propaga...

  • Article
  • Open Access
3 Citations
1,172 Views
20 Pages

Optimizing In Vitro Propagation of “Ladania” (Cistus creticus L.) Through Interaction of Light Spectra and Plant Growth Regulators

  • Chrysanthi Evangelia Katsanou,
  • Stefanos Kostas,
  • Filippos Bantis,
  • Konstantinos Bertsouklis and
  • Stefanos Hatzilazarou

22 March 2025

This study investigates the impact of light conditions on the growth, rooting, and photosynthetic performance of in vitro cultivated Cistus creticus L. explants. Initially, different plant growth regulators were tested for multiple shoot growth [5 an...

  • Article
  • Open Access
12 Citations
5,396 Views
10 Pages

14 April 2020

A number of Aloe species are facing an extremely high risk of extinction due to habitat loss and over-exploitation for medicinal and ornamental trade. The last global assessment of Aloe peglerae Schönland (in 2003) ranked its global conservation...

  • Article
  • Open Access
329 Views
14 Pages

In Vitro Propagation of Sweet Rowanberry Cultivar Discolor as an Alternative Fruit Crop Resource

  • Jiří Sedlák,
  • Martin Mészáros,
  • Liliia Pavliuk,
  • Michaela Marklová and
  • Boris Krška

7 December 2025

Developing reliable in vitro propagation methods for rowanberry genotypes is essential for their use in breeding and horticultural programs. While different rowanberry species and cultivars are primarily valued for their ornamental and forestry prope...

  • Article
  • Open Access
2,609 Views
12 Pages

23 October 2022

Carpesium divaricatum Sieb. & Zucc. is a plant species rich in terpenoids of anti-inflammatory and cytotoxic activity, especially germacranolides of potential medicinal value. The present study describes in vitro multiplication of C. divaric...

  • Article
  • Open Access
3 Citations
2,695 Views
20 Pages

7 November 2023

The Jerusalem artichoke (Helianthus tuberosus) is a tuberous plant with considerable nutrient and bioactive compounds. The optimization of the in vitro clonal propagation protocol is critical for large-scale reproduction and biotechnological applicat...

  • Article
  • Open Access
2 Citations
1,553 Views
11 Pages

9 December 2024

Optimizing concentrations of minerals and phytohormones is essential when culturing a new plant species. The objective of this study was to use Response Surface Methodology (RSM) to evaluate combinations of selected minerals (N, Ca, and P) along with...

  • Article
  • Open Access
6 Citations
5,239 Views
15 Pages

Temporary Immersion Bioreactor (TIB) System for Large-Scale Micropropagation of Musa sp. cv Kluai Numwa Pakchong 50

  • Sudarat Thanonkeo,
  • Haruthairat Kitwetcharoen,
  • Pornthap Thanonkeo and
  • Preekamol Klanrit

27 September 2024

Conventional in vitro propagation using semisolid Murashige and Skoog (MS) culture systems is costly, labor-intensive, and requires substantial space for large-scale plant production. This study investigated the application of a temporary immersion b...

  • Article
  • Open Access
1 Citations
1,098 Views
15 Pages

Role of Plant Growth Regulators in Adventitious Populus Tremula Root Development In Vitro

  • Miglė Vaičiukynė,
  • Jonas Žiauka,
  • Valentinas Černiauskas and
  • Iveta Varnagirytė-Kabašinskienė

5 August 2025

Eurasian aspen (Populus tremula L.) is a tree species with recognised ecological and economic importance for both natural and plantation forests. For the fast cloning of selected aspen genotypes, the method of plant propagation through in vitro cultu...

  • Article
  • Open Access
5 Citations
2,377 Views
19 Pages

Fine-Tuning of Arabidopsis thaliana Response to Endophytic Colonization by Gluconacetobacter diazotrophicus PAL5 Revealed by Transcriptomic Analysis

  • Fabiano Silva Soares,
  • Ana Lídia Soares Rangel de Souza,
  • Suzane Ariádina de Souza,
  • Luciano de Souza Vespoli,
  • Vitor Batista Pinto,
  • Lucia Matiello,
  • Felipe Rodrigues da Silva,
  • Marcelo Menossi and
  • Gonçalo Apolinário de Souza Filho

21 June 2024

Gluconacetobacter diazotrophicus is a diazotrophic endophytic bacterium that promotes the growth and development of several plant species. However, the molecular mechanisms activated during plant response to this bacterium remain unclear. Here, we us...

  • Article
  • Open Access
150 Citations
10,475 Views
15 Pages

31 August 2015

Glucosinolate (GSL) profiles and concentrations in various tissues (seeds, sprouts, mature root, and shoot) were determined and compared across nine Brassica species, including cauliflower, cabbage, broccoli, radish, baemuchae, pakchoi, Chinese cabba...

  • Article
  • Open Access
39 Citations
7,609 Views
13 Pages

A Study of Proline Metabolism in Canola (Brassica napus L.) Seedlings under Salt Stress

  • Mubshara Saadia,
  • Amer Jamil,
  • Nudrat Aisha Akram and
  • Muhammad Ashraf

16 May 2012

Expression analysis of crop plants has improved our knowledge about the veiled underlying mechanisms for salt tolerance. In order to observe the time course effects of salinity stress on gene expression for enzymes regulating proline metabolism, we c...

  • Article
  • Open Access
12 Citations
3,926 Views
12 Pages

Optimization of High-Efficiency Tissue Culture Regeneration Systems in Gray Poplar

  • Huanhuan Li,
  • Hang Wang,
  • Lianke Guan,
  • Zihui Li,
  • Hua Wang and
  • Jie Luo

11 September 2023

A series of tissue culture regeneration protocols were conducted on gray poplar (P. tremula × P. alba) to select the most efficient callus induction medium, adventitious shoot differentiation medium, shoot elongation medium and rooting medium,...

  • Article
  • Open Access
4 Citations
2,673 Views
18 Pages

Enhanced Carbonylation of Photosynthetic and Glycolytic Proteins in Antibiotic Timentin-Treated Tobacco In Vitro Shoot Culture

  • Elena Andriūnaitė,
  • Rytis Rugienius,
  • Inga Tamošiūnė,
  • Perttu Haimi,
  • Jurgita Vinskienė and
  • Danas Baniulis

14 June 2022

Antibiotics are used in plant in vitro tissue culture to eliminate microbial contamination or for selection in genetic transformation. Antibiotic timentin has a relatively low cytotoxic effect on plant tissue culture; however, it could induce an endu...

  • Review
  • Open Access
68 Citations
12,746 Views
35 Pages

Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs

  • Martin Raspor,
  • Václav Motyka,
  • Abdul Rasheed Kaleri,
  • Slavica Ninković,
  • Ljiljana Tubić,
  • Aleksandar Cingel and
  • Tatjana Ćosić

De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media supplemented with the plant hormones cytokinin (CK) and auxin, which...

  • Article
  • Open Access
5 Citations
3,940 Views
9 Pages

Optimized Regeneration of Petunia Protoplast and Its Association with Tissue Identity Regulators

  • Luhua Tu,
  • Saminathan Subburaj,
  • Kayoun Lee,
  • Yongsam Jeon,
  • Fanzhuang Yan,
  • Jian Yao,
  • Young-Sun Kim,
  • Ok-Jae Koo and
  • Geung-Joo Lee

The popular ornamental plant Petunia is also a valuable model plant in tissue culture. Cellular conversions during differentiation and regeneration have been investigated using various combinations of phytohormones; however, studies on genes for repr...

  • Article
  • Open Access
10 Citations
2,580 Views
12 Pages

The Response of Thiols to Cadmium Stress in Spinach (Spinacia Oleracea L.)

  • Ya Gao,
  • Haipu Li,
  • Yang Song,
  • Fenglin Zhang and
  • Zhaoguang Yang

28 July 2022

The aim of this study is to examine the thiol species for the high cadmium (Cd) tolerance of spinach and provide information for the improvement of soil utilization. The spinach was cultured in aqueous solution with concentrations of Cd ranging from...

  • Communication
  • Open Access
884 Views
10 Pages

7 April 2025

In October 2024, a large-scale blight was found on tender shoots (young green shoots) of Juniperus formosana in Kaili City, Guizhou Province, China. The pathogen was isolated and purified from the diseased tissues by the tissue isolation technique. T...

  • Article
  • Open Access
12 Citations
5,557 Views
13 Pages

Gluconacetobacter diazotrophicus Inoculation of Two Lettuce Cultivars Affects Leaf and Root Growth under Hydroponic Conditions

  • Ryan L. Sebring,
  • Sjoerd W. Duiker,
  • Robert D. Berghage,
  • John M. Regan,
  • Joshua D. Lambert and
  • Ray B. Bryant

1 February 2022

The growth-promoting effects of Gluconacetobacter diazotrophicus inoculation on the leaf lettuce (Lactuca sativa L.) cultivars “Black Seeded Simpson” and “Bibb/Limestone” were investigated. Plants of each cultivar were grown h...

  • Article
  • Open Access
8 Citations
4,825 Views
18 Pages

Enduring Effect of Antibiotic Timentin Treatment on Tobacco In Vitro Shoot Growth and Microbiome Diversity

  • Inga Tamošiūnė,
  • Elena Andriūnaitė,
  • Jurgita Vinskienė,
  • Vidmantas Stanys,
  • Rytis Rugienius and
  • Danas Baniulis

21 March 2022

Plant in vitro cultures initiated from surface-sterilized explants often harbor complex microbial communities. Antibiotics are commonly used to decontaminate plant tissue culture or during genetic transformation; however, the effect of antibiotic tre...

  • Article
  • Open Access
12 Citations
3,639 Views
15 Pages

Tracking Permeation of Dimethyl Sulfoxide (DMSO) in Mentha × piperita Shoot Tips Using Coherent Raman Microscopy

  • Heidi D. Kreckel,
  • Fionna M. D. Samuels,
  • Remi Bonnart,
  • Gayle M. Volk,
  • Dominik G. Stich and
  • Nancy E. Levinger

8 June 2023

Cryopreservation has emerged as a low-maintenance, cost-effective solution for the long-term preservation of vegetatively propagated crops. Shoot tip cryopreservation often makes use of vitrification methods that employ highly concentrated mixtures o...

  • Article
  • Open Access
8 Citations
3,190 Views
15 Pages

Lingonberry (Vaccinium vitis-idaea L.) is an important and valuable horticultural crop due to its high antioxidant properties. Plant tissue culture is an advanced propagation system employed in horticultural crops. However, the progeny derived using...

  • Article
  • Open Access
21 Citations
5,086 Views
13 Pages

Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

  • Han Yong Park,
  • Doo Hwan Kim,
  • Ramesh Kumar Saini,
  • Judy Gopal,
  • Young-Soo Keum and
  • Iyyakkannu Sivanesan

The goal of this study was to establish an efficient protocol for the large-scale propagation of Mertensia maritima (L.) Gray, and evaluate the carotenoid, fatty acid, and tocopherol contents in the leaves of in vitro regenerated shoots. Surface-disi...

  • Brief Report
  • Open Access
1 Citations
3 Pages

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next ge...

  • Article
  • Open Access
31 Citations
3,982 Views
12 Pages

5 May 2021

Plant damage caused by defoliating insects has a long-term negative effect on plant growth and productivity. Consequently, the restoration of plant growth after exposure to pathogens or pests is the main indicator of the effectiveness of the implemen...

  • Article
  • Open Access
2 Citations
2,839 Views
12 Pages

4 July 2023

Cacti have a distinct adaptation to arid conditions with a massive water storing tissue surrounding a weak central woody cylinder. However, they have not been studied as extensively as other plants have been. Selenicereus undatus is a hemi-epiphytic...

  • Article
  • Open Access
6 Citations
5,056 Views
19 Pages

Plant Regeneration Protocol for Recalcitrant Passionflower (Passiflora quadrangularis L.)

  • Paula Boboc Oros,
  • Maria Cantor,
  • Mirela Irina Cordea and
  • Corina Cătană

This research was designed to provide the first protocol to establish an efficient solution for direct organogenesis regeneration in Passiflora quadrangularis using nodal explants from young shoots. Passifloraceae tissue culture has been associated w...

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