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Article

Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development

1
Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Rishon LeZion 7505101, Israel
2
The Robert H. Smith Faculty of Agriculture, Food and Environment, The Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel
*
Author to whom correspondence should be addressed.
Academic Editor: Mikihisa Umehara
Plants 2022, 11(8), 1035; https://doi.org/10.3390/plants11081035
Received: 9 March 2022 / Revised: 7 April 2022 / Accepted: 9 April 2022 / Published: 11 April 2022
(This article belongs to the Section Plant Development and Morphogenesis)
Sucrose synthase (SuSy) and fructokinase (FRK) work together to control carbohydrate flux in sink tissues. SuSy cleaves sucrose into fructose and UDP-glucose; whereas FRK phosphorylates fructose. Previous results have shown that suppression of the SUS1,3&4 genes by SUS-RNAi alters auxin transport in the shoot apical meristems of tomato plants and affects cotyledons and leaf structure; whereas antisense suppression of FRK2 affects vascular development. To explore the joint developmental roles of SuSy and FRK, we crossed SUS-RNAi plants with FRK2-antisense plants to create double-mutant plants. The double-mutant plants exhibited novel phenotypes that were absent from the parent lines. About a third of the plants showed arrested shoot apical meristem around the transition to flowering and developed ectopic meristems. Use of the auxin reporter DR5::VENUS revealed a significantly reduced auxin response in the shoot apical meristems of the double-mutant, indicating that auxin levels were low. Altered inflorescence phyllotaxis and significant disorientation of vascular tissues were also observed. In addition, the fruits and the seeds of the double-mutant plants were very small and the seeds had very low germination rates. These results show that SUS1,3&4 and FRK2 enzymes are jointly essential for proper meristematic and vascular development, and for fruit and seed development. View Full-Text
Keywords: auxin; phyllotaxis; shoot apical meristem; sugar metabolism; trichomes density; vascular tissues development auxin; phyllotaxis; shoot apical meristem; sugar metabolism; trichomes density; vascular tissues development
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MDPI and ACS Style

Lugassi, N.; Stein, O.; Egbaria, A.; Belausov, E.; Zemach, H.; Arad, T.; Granot, D.; Carmi, N. Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development. Plants 2022, 11, 1035. https://doi.org/10.3390/plants11081035

AMA Style

Lugassi N, Stein O, Egbaria A, Belausov E, Zemach H, Arad T, Granot D, Carmi N. Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development. Plants. 2022; 11(8):1035. https://doi.org/10.3390/plants11081035

Chicago/Turabian Style

Lugassi, Nitsan, Ofer Stein, Aiman Egbaria, Eduard Belausov, Hanita Zemach, Tal Arad, David Granot, and Nir Carmi. 2022. "Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development" Plants 11, no. 8: 1035. https://doi.org/10.3390/plants11081035

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