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Brief Report

Sample Preparation for Laser-Microdissection of Soybean Shoot Apical Meristem

Plant Molecular Biology and Biotechnology Group, ARC Centre of Excellence for Integrative Legume Research, Melbourne School of Land and Environment, The University of Melbourne, Parkville, VIC 3010, Victoria
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Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2012, 3(1), e3; https://doi.org/10.4081/pb.2012.e3
Submission received: 19 October 2011 / Revised: 12 March 2012 / Accepted: 6 April 2012 / Published: 16 October 2012

Abstract

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next generation sequencing permits an in-depth analysis of molecular events associated with specific cell type of interest. Sample preparation is the most critical step in ensuring good quality RNA to be extracted from samples following laser-microdissection. Here, we optimized the sample preparation for a major legume crop, soybean. We used Farmer’s solution as a fixative and paraffin as the embedding medium for soybean shoot apical meristem tissue without the use of any specialized equipment. Shorter time for tissue fixation (two days) was found to be critical for the preservation of RNA in soybean shoot apical meristem. We further demonstrated the utility of this method for different tissues derived from soybean and rice. The method outlined here shall facilitate studies on crop plants involving laser-microdissection.
Keywords: laser-microdissection; shoot apical meristem; soybean laser-microdissection; shoot apical meristem; soybean

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MDPI and ACS Style

Wong, C.E.; Singh, M.B.; Bhalla, P.L. Sample Preparation for Laser-Microdissection of Soybean Shoot Apical Meristem. Int. J. Plant Biol. 2012, 3, e3. https://doi.org/10.4081/pb.2012.e3

AMA Style

Wong CE, Singh MB, Bhalla PL. Sample Preparation for Laser-Microdissection of Soybean Shoot Apical Meristem. International Journal of Plant Biology. 2012; 3(1):e3. https://doi.org/10.4081/pb.2012.e3

Chicago/Turabian Style

Wong, Chui E., Mohan B. Singh, and Prem L. Bhalla. 2012. "Sample Preparation for Laser-Microdissection of Soybean Shoot Apical Meristem" International Journal of Plant Biology 3, no. 1: e3. https://doi.org/10.4081/pb.2012.e3

APA Style

Wong, C. E., Singh, M. B., & Bhalla, P. L. (2012). Sample Preparation for Laser-Microdissection of Soybean Shoot Apical Meristem. International Journal of Plant Biology, 3(1), e3. https://doi.org/10.4081/pb.2012.e3

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