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  • Article
  • Open Access
22 Citations
5,555 Views
12 Pages

Wastewater-Based Epidemiology for Viral Surveillance from an Endemic Perspective: Evidence and Challenges

  • Marco Verani,
  • Alessandra Pagani,
  • Ileana Federigi,
  • Giulia Lauretani and ,
  • Nebiyu Tariku Atomsa,
  • Virginia Rossi,
  • Luca Viviani and
  • Annalaura Carducci
  • + 3 authors+ 0 author

20 March 2024

Wastewater-based epidemiology (WBE) is currently used to monitor not only the spread of the viral SARS-CoV-2 pandemic but also that of other viruses in endemic conditions, particularly in the absence of syndromic surveillance. The continuous monitori...

(This article belongs to the Special Issue Virology in Italy 2023: National Congress of the Italian Society for Virology)
  • Review
  • Open Access
23 Citations
7,400 Views
26 Pages

Wastewater-based epidemiology (WBE) has emerged as a transformative approach for community-level health monitoring, particularly during the COVID-19 pandemic. This review critically examines the methodological framework of WBE systems through the fol...

(This article belongs to the Special Issue The Molecular Epidemiology of Infectious Diseases)
  • Review
  • Open Access
10 Citations
3,460 Views
14 Pages

Passive Sampler Technology for Viral Detection in Wastewater-Based Surveillance: Current State and Nanomaterial Opportunities

  • Alberto Aguayo-Acosta,
  • Mildred G. Jiménez-Rodríguez,
  • Fernando Silva-Lance,
  • Mariel Araceli Oyervides-Muñoz and ,
  • Arnoldo Armenta-Castro,
  • Orlado de la Rosa,
  • Antonio Ovalle-Carcaño,
  • Elda M. Melchor-Martínez,
  • Zahra Aghalari and
  • Juan Eduardo Sosa-Hernández
  • + 6 authors+ 1 author

16 September 2023

Although wastewater-based surveillance (WBS) is an efficient community-wide surveillance tool, its implementation for pathogen surveillance remains limited by ineffective sample treatment procedures, as the complex composition of wastewater often int...

(This article belongs to the Special Issue Nanotechnological Applications in Virology 2023)
  • Article
  • Open Access
3 Citations
2,830 Views
21 Pages

High-Resolution Wastewater-Based Surveillance of Three Influenza Seasons (2022–2025) Reveals Distinct Seasonal Patterns of Viral Activity in Munich, Germany

  • Jessica Neusser,
  • Astrid Zierer,
  • Anna Riedl,
  • Jasmin Javanmardi and ,
  • Raquel Rubio-Acero,
  • Elisabeth Waldeck,
  • Thomas Kletke,
  • Annemarie Bschorer,
  • Stefanie Huber and
  • Andreas Wieser
  • + 14 authors+ 9 authors

In the Northern Hemisphere, annual waves of influenza disease with varying degrees of spread and severity are observed each winter. With wastewater-based surveillance (WBS), including both centralized (one wastewater treatment plant, WWTP) and decent...

(This article belongs to the Special Issue Surveillance of Health-Relevant Pathogens Employing Wastewater)
  • Review
  • Open Access
21 Citations
7,552 Views
21 Pages

14 August 2024

This review article will present a comprehensive examination of the use of modeling, spatial analysis, and geographic information systems (GIS) in the surveillance of viruses in wastewater. With the advent of global health challenges like the COVID-1...

(This article belongs to the Special Issue Viruses in Water)
  • Article
  • Open Access
16 Citations
7,869 Views
17 Pages

Effective Inhibitor Removal from Wastewater Samples Increases Sensitivity of RT-dPCR and Sequencing Analyses and Enhances the Stability of Wastewater-Based Surveillance

  • Nico Linzner,
  • Alexander Bartel,
  • Vera Schumacher,
  • José Horacio Grau and ,
  • Emanuel Wyler,
  • Henrike Preuß,
  • Sonja Garske,
  • Julia Bitzegeio,
  • Elisabeth Barbara Kirst and
  • Uta Böckelmann
  • + 12 authors+ 7 authors

Wastewater-based surveillance (WBS) is a proven tool for monitoring population-level infection events. Wastewater contains high concentrations of inhibitors, which contaminate the total nucleic acids (TNA) extracted from these samples. We found that...

(This article belongs to the Special Issue Surveillance of SARS-CoV-2 Employing Wastewater)
  • Article
  • Open Access
2 Citations
3,005 Views
15 Pages

Culture-Based Wastewater Surveillance for the Detection and Monitoring of Antimicrobial Resistance in Staphylococcal Species

  • Douha Shouqair,
  • Rashed Alghafri,
  • Mohammed Naji,
  • Abdulla Albastaki and ,
  • Rania Nassar,
  • Lobna Mohamed,
  • Bisola Aloba,
  • Bayan S. Awad,
  • Fatima Al Dhaheri and
  • Abiola Senok
  • + 15 authors+ 10 authors

23 December 2025

Wastewater-based surveillance (WBS) is valuable for monitoring antimicrobial resistance (AMR). Staphylococci are key targets, as wastewater can facilitate gene transfer and resistance emergence. Data on WBS for population-level AMR in the Arabian-Gul...

  • Article
  • Open Access
25 Citations
3,809 Views
9 Pages

Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance

  • Oran Erster,
  • Itay Bar-Or,
  • Virginia Levy,
  • Rachel Shatzman-Steuerman and ,
  • Danit Sofer,
  • Leah Weiss,
  • Rinat Vasserman,
  • Ilana S. Fratty,
  • Klil Kestin and
  • Merav Weil
  • + 10 authors+ 5 authors

9 May 2022

Enterovirus D68 (EVD68) was recently identified as an important cause of respiratory illness and acute flaccid myelitis (AFM), mostly in children. Here, we examined 472 pediatric patients diagnosed with severe respiratory illness and screened for EVD...

(This article belongs to the Topic Wastewater-Based Epidemiology)
  • Article
  • Open Access
1 Citations
2,159 Views
15 Pages

Wastewater-Based Surveillance of Respiratory Viruses in the SARS-CoV-2 Post-Pandemic Period in Mexico

  • Oscar Uriel Ulloa-Medina,
  • Pedro Gerardo Hernández-Sánchez,
  • Gabriel Mata-Moreno,
  • Luis Rubén Jaime-Rocha and ,
  • Sara del Carmen Alatorre-Camacho,
  • Ignacio Lara-Hernández,
  • Mauricio Comas-García,
  • Juan Carlos Muñoz-Escalante,
  • Ana María González-Ortiz and
  • Daniel E. Noyola
  • + 6 authors+ 1 author

17 February 2026

In recent years, wastewater (WW)-based epidemiology has been increasingly used for surveillance of SARS-CoV-2 and has emerged as a potential tool for monitoring other respiratory viruses. Most evidence on the use of WW for detecting multiple respirat...

(This article belongs to the Special Issue Wastewater-Based Epidemiology and Viral Surveillance)
  • Editorial
  • Open Access
519 Views
12 Pages

Wastewater-based epidemiology (WBE) and the closely related practice of wastewater-based surveillance (WBS) have moved from specialized environmental monitoring approaches to increasingly important components of public health surveillance [...]

(This article belongs to the Special Issue Wastewater-Based Epidemiology Assessment and Surveillance)
  • Article
  • Open Access
1 Citations
1,161 Views
18 Pages

Comparative Genomics of Human- and Wastewater-Derived CPE Isolates in The Netherlands Reveals Shared and Complementary Characteristics

  • Hetty Blaak,
  • Sandra Witteveen,
  • Angela de Haan,
  • Marga G. van Santen-Verheuvel and ,
  • Merel A. Kemper,
  • Ana Maria de Roda Husman,
  • Antoni P. A. Hendrickx,
  • Heike Schmitt and
  • The Dutch CPE Surveillance Study Group
  • + 4 authors+ 0 author

Wastewater-based surveillance is gaining interest worldwide as a complementary tool informing human surveillance of pathogens, among which are antibiotic-resistant bacteria. The current study investigated whether CPE detected within the Dutch human C...

(This article belongs to the Special Issue From Sewers to Solutions: Wastewater Surveillance in the Fight Against Antimicrobial Resistance)
  • Article
  • Open Access
14 Citations
5,676 Views
12 Pages

Extensive Wastewater-Based Epidemiology as a Resourceful Tool for SARS-CoV-2 Surveillance in a Low-to-Middle-Income Country through a Successful Collaborative Quest: WBE, Mobility, and Clinical Tests

  • Juan Eduardo Sosa-Hernández,
  • Mariel Araceli Oyervides-Muñoz,
  • Elda M. Melchor-Martínez,
  • Erin M. Driver and ,
  • Devin A. Bowes,
  • Simona Kraberger,
  • Sofia Liliana Lucero-Saucedo,
  • Rafaela S. Fontenele,
  • Lizeth Parra-Arroyo and
  • Roberto Parra-Saldívar
  • + 19 authors+ 14 authors

8 June 2022

The COVID-19 pandemic has challenged healthcare systems worldwide. Efforts in low-to-middle-income countries (LMICs) cannot keep stride with infection rates, especially during peaks. A strong international collaboration between Arizona State Universi...

(This article belongs to the Special Issue Pathogen Detection and Identification in Wastewater)
  • Review
  • Open Access
6 Citations
5,762 Views
22 Pages

A Narrative Review of High Throughput Wastewater Sample Processing for Infectious Disease Surveillance: Challenges, Progress, and Future Opportunities

  • Bhuvanesh Kumar Shanmugam,
  • Maryam Alqaydi,
  • Degan Abdisalam,
  • Monika Shukla and ,
  • Helio Santos,
  • Ranya Samour,
  • Lawrence Petalidis,
  • Charles Matthew Oliver,
  • Grzegorz Brudecki and
  • Wael Elamin
  • + 6 authors+ 1 author

During the recent COVID-19 pandemic, wastewater-based epidemiological (WBE) surveillance played a crucial role in evaluating infection rates, analyzing variants, and identifying hot spots in a community. This expanded the possibilities for using wast...

(This article belongs to the Special Issue Pandemic Preparedness: Lessons Learned from COVID-19)
  • Systematic Review
  • Open Access
2 Citations
2,588 Views
38 Pages

Wastewater as Sentinel for Emerging Viral Diseases in Livestock: A Systematic Review

  • Mishuk Shaha,
  • Ashutosh Das,
  • Joyshri Saha,
  • Md. Mizanur Rahaman and ,
  • Mukta Das Gupta,
  • Saranika Talukder and
  • Subir Sarker
  • + 2 authors+ 0 author

19 March 2026

The accelerating frequency of emerging infectious diseases (EIDs) in livestock poses a significant threat to global food security, as well as to animal and public health. While wastewater-based surveillance (WBS) has advanced significantly for human...

(This article belongs to the Section Animal Viruses)
  • Article
  • Open Access
7 Citations
2,067 Views
20 Pages

Evaluating Interlaboratory Variability in Wastewater-Based COVID-19 Surveillance

  • Arianna Azzellino,
  • Laura Pellegrinelli,
  • Ramon Pedrini,
  • Andrea Turolla and ,
  • Barbara Bertasi,
  • Sandro Binda,
  • Sara Castiglioni,
  • Clementina E. Cocuzza,
  • Fabio Ferrari and
  • Francesca Malpei
  • + 20 authors+ 15 authors

Wastewater-based environmental surveillance enables the monitoring of SARS-CoV-2 dynamics within populations, offering critical epidemiological insights. Numerous workflows for tracking SARS-CoV-2 have been developed globally, underscoring the need f...

(This article belongs to the Special Issue Surveillance of SARS-CoV-2 Employing Wastewater)
  • Article
  • Open Access
13 Citations
3,097 Views
13 Pages

Wastewater-Based Epidemiology as a Tool to Detect SARS-CoV-2 Circulation at the Community Level: Findings from a One-Year Wastewater Investigation Conducted in Sicily, Italy

  • Carmelo Massimo Maida,
  • Fabio Tramuto,
  • Giovanni Maurizio Giammanco,
  • Roberta Palermo and ,
  • Walter Priano,
  • Simona De Grazia,
  • Giuseppa Purpari,
  • Giuseppina La Rosa,
  • Elisabetta Suffredini and
  • Walter Mazzucco
  • + 12 authors+ 7 authors

Wastewater-based epidemiology is a well-established tool for detecting and monitoring the spread of enteric pathogens and the use of illegal drugs in communities in real time. Since only a few studies in Italy have investigated the correlation betwee...

(This article belongs to the Special Issue Epidemiology of Emerging Infectious Disease: Importance of Surveillance and Detection in Public Health Initiatives)
  • Review
  • Open Access
27 Citations
8,881 Views
19 Pages

The Application of Passive Sampling Devices in Wastewater Surveillance

  • Andreana G. Shakallis,
  • Howard Fallowfield,
  • Kirstin E. Ross and
  • Harriet Whiley

31 October 2022

Wastewater-based epidemiology (WBE) is a surveillance approach used to examine chemical and biological targets within a population. Historically, the most common approaches to wastewater sampling include grab sampling and composite sampling, which ca...

(This article belongs to the Section Wastewater Treatment and Reuse)
  • Article
  • Open Access
8 Citations
5,443 Views
18 Pages

Metagenomic Meta-Analysis of Antibiotic-Resistance Genes in Wastewater: A Perspective from the COVID-19 Pandemic

  • Shaima M. Alhazmi,
  • Ala’a BaniMustafa,
  • Abrar R. Alindonosi and
  • Adel F. Almutairi

12 December 2024

Antibiotic resistance is a silent global crisis intensified by the recent pandemic of coronavirus disease 2019 (COVID-19). To address this growing threat, wastewater-based surveillance (WBS) is emerging as a promising public health tool for monitorin...

(This article belongs to the Section Wastewater Treatment and Reuse)
  • Article
  • Open Access
47 Citations
7,507 Views
12 Pages

Expanding the Pathogen Panel in Wastewater Epidemiology to Influenza and Norovirus

  • Rudolf Markt,
  • Fabian Stillebacher,
  • Fabiana Nägele,
  • Anna Kammerer and ,
  • Nico Peer,
  • Maria Payr,
  • Christoph Scheffknecht,
  • Silvina Dria,
  • Simon Draxl-Weiskopf and
  • Andreas Otto Wagner
  • + 14 authors+ 9 authors

17 January 2023

Since the start of the 2019 pandemic, wastewater-based epidemiology (WBE) has proven to be a valuable tool for monitoring the prevalence of SARS-CoV-2. With methods and infrastructure being settled, it is time to expand the potential of this tool to...

(This article belongs to the Special Issue Wastewater-Based Epidemiology (WBE) in COVID-19 Pandemics)
  • Perspective
  • Open Access
15 Citations
5,598 Views
16 Pages

A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries

  • Mohammad Shehryaar Khan,
  • Christian Wurzbacher,
  • Anna Uchaikina,
  • Boris Pleshkov and ,
  • Olga Mirshina and
  • Jörg E. Drewes
  • + 1 author+ 0 author

Geographical variations in infectious diseases create differences in public health priorities between high- and low-income countries. Low- and middle-income countries (LMICs) face resource constraints that limit adherence to international monitoring...

(This article belongs to the Special Issue Surveillance of SARS-CoV-2 Employing Wastewater)
  • Systematic Review
  • Open Access
726 Views
22 Pages

In Africa, the disease burden of diarrheal and respiratory diseases is amplified by limited surveillance capacity, diagnostic limitations, and socioeconomic inequalities. In rapidly urbanizing settings such as Kigali (Rwanda), integrating wastewater-...

  • Article
  • Open Access
1,175 Views
13 Pages

Wastewater-Based Surveillance of SARS-CoV-2 for Early Warning of COVID-19 Infection Dynamics

  • Qiuyan Zhao,
  • Xinye Zhang,
  • Jing Peng,
  • Xiaoyan Ma and ,
  • Yongxing Wang,
  • Jun Luo,
  • Xiaohan Su,
  • Siyu Yang,
  • Xiaona Yan and
  • Jie Zhang
  • + 6 authors+ 1 author

18 May 2026

Wastewater-based epidemiology has emerged as a valuable complementary tool for population-level monitoring. This study evaluated the early warning value of wastewater surveillance for monitoring SARS-CoV-2 and its correlation with COVID-19 infection...

(This article belongs to the Section Human Virology and Viral Diseases)
  • Article
  • Open Access
2 Citations
3,825 Views
19 Pages

Australian and New Zealand Laboratory Experience and Proposed Future Direction of Wastewater Pathogen Genomic Surveillance

  • Avram Levy,
  • Christina Crachi,
  • Jake Gazeley,
  • Joanne Chapman and ,
  • Anna Brischetto,
  • David Speers,
  • Joanne Hewitt,
  • Amy V. Jennison and
  • The Wastewater Surveillance Working Group, Communicable Diseases Genomics Network of Australia
  • + 4 authors+ 0 author

Wastewater pathogen surveillance was rapidly implemented across Australia and New Zealand as a public health tool during the COVID-19 pandemic. To assess method consistency and identify opportunities for harmonization, we surveyed all Australian and...

(This article belongs to the Special Issue Environments: 10 Years of Science Together)
  • Review
  • Open Access
1 Citations
1,066 Views
27 Pages

11 April 2026

Wastewater-based epidemiology (WBE) has emerged as a powerful approach for population-level monitoring of chemical exposure, health status, and disease transmission by analysing wastewater. Although chromatographic and molecular techniques remain the...

(This article belongs to the Section Biosensors)
  • Article
  • Open Access
12 Citations
3,690 Views
14 Pages

Potential Use of Untreated Wastewater for Assessing COVID-19 Trends in Southern Italy

  • Osvalda De Giglio,
  • Francesco Triggiano,
  • Francesca Apollonio,
  • Giusy Diella and ,
  • Fabrizio Fasano,
  • Pasquale Stefanizzi,
  • Marco Lopuzzo,
  • Silvia Brigida,
  • Carla Calia and
  • Maria Teresa Montagna
  • + 12 authors+ 7 authors

As a complement to clinical disease surveillance, the monitoring of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in wastewater can be used as an early warning system for impending epidemics. This study investigated the dynamics of SAR...

(This article belongs to the Special Issue Environmental Hygiene, Water Quality and Human Health)
  • Article
  • Open Access
9 Citations
3,855 Views
19 Pages

Wastewater-Based Surveillance of SARS-CoV-2 and Modeling of COVID-19 Infection Trends

  • Wenli Wang,
  • Ruoyu Li,
  • Shilin Chen,
  • Liangping Chen and ,
  • Yu Jiang,
  • Jianjun Xiang,
  • Jing Wu,
  • Jing Li,
  • Zhiwei Chen and
  • Chuancheng Wu
  • + 5 authors+ 0 author

Background: This study was performed to evaluate the early warning value of wastewater-based epidemiology (WBE) in monitoring severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its correlation with population-level coronavirus disease 2...

(This article belongs to the Section Infectious Diseases)
  • Systematic Review
  • Open Access
1 Citations
3,310 Views
22 Pages

Methodological Approaches to Dengue Virus Detection in Wastewater: A Systematic Review and Meta-Analysis of Positivity Rate

  • Siti Aishah Rashid,
  • Sakshaleni Rajendiran,
  • Nurul Farehah Shahrir,
  • Nurul Athirah Naserrudin and ,
  • Terence Tan Yew Chin,
  • Janice Chan Sue Wen,
  • Imanul Hassan Abdul Shukor and
  • Nurul Amalina Khairul Hasni
  • + 3 authors+ 0 author

30 April 2026

Dengue fever, with a high proportion of asymptomatic infections, poses a major global public health challenge that traditional surveillance systems frequently underestimate. Wastewater-based epidemiology (WBE) has emerged as a promising approach to m...

(This article belongs to the Section General Virology)
  • Article
  • Open Access
6 Citations
2,597 Views
11 Pages

Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany

  • Marco Verani,
  • Ileana Federigi,
  • Sara Muzio,
  • Giulia Lauretani and ,
  • Piergiuseppe Calà,
  • Fabrizio Mancuso,
  • Roberto Salvadori,
  • Claudia Valentini,
  • Giuseppina La Rosa and
  • Annalaura Carducci
  • + 6 authors+ 1 author

The current pandemic has provided an opportunity to test wastewater-based epidemiology (WBE) as a complementary method to SARS-CoV-2 monitoring in the community. However, WBE infection estimates can be affected by uncertainty factors, such as heterog...

(This article belongs to the Special Issue Public Health Implications Relating to Microbiological Pollution of Water)
  • Article
  • Open Access
2 Citations
2,545 Views
15 Pages

Assessing Virus Concentration Methods for Norovirus and SARS-CoV-2 Detection in Wastewater

  • Rakshya Baral,
  • Daniel A. Nwaubani,
  • Tamunobelema Solomon and
  • Samendra P. Sherchan

Polyethylene Glycol (PEG) precipitation and Nanotrap® Microbiome Particles (NMP) are widely used methods for concentrating viruses in wastewater due to their simplicity, cost-effectiveness, efficiency, and rapid turnaround time. This study compar...

(This article belongs to the Special Issue Wastewater-Based Epidemiology Assessment and Surveillance)
  • Article
  • Open Access
1 Citations
6,250 Views
15 Pages

Correlating Quantitative and Genomic SARS-CoV-2 Wastewater Data with Clinical Metrics in Metropolitan Perth, Western Australia

  • Jake Gazeley,
  • Terence Lee,
  • Daniel R. Knight,
  • Alexander Shivarev and ,
  • Cameron Gordon,
  • David Speers,
  • Dylan D. Barth,
  • Jelena Maticevic,
  • Meredith Hodge and
  • Avram Levy
  • + 8 authors+ 3 authors

Wastewater-based epidemiology (WBE) has emerged as a key method for the continuous monitoring of COVID-19 prevalence including circulating SARS-CoV-2 lineages. WBE addresses the limitations of traditional clinical COVID-19 surveillance such as clinic...

  • Article
  • Open Access
1,214 Views
16 Pages

Wastewater-Based Detection of a Rare SARS-CoV-2 Variant in a Hospital Setting: Implications for Individual-Level Resolution

  • Jeffrey L. Ram,
  • Md Alamin,
  • Azadeh Bahmani,
  • Lance Gable and ,
  • Sneha Ghosh,
  • Katherine Gurdziel,
  • James Hartrick,
  • Natasha Islam,
  • Paul E. Kilgore and
  • Wanqing Liu
  • + 18 authors+ 13 authors

Wastewater-based surveillance is an effective method to monitor community health by detecting pathogens excreted from large populations. Traditionally, wastewater analyses are considered privacy-preserving since samples are pooled. However, advances...

(This article belongs to the Special Issue Wastewater-Based Epidemiology Assessment and Surveillance)
  • Article
  • Open Access
723 Views
20 Pages

Comparative Analysis of Viral Communities in Hospital, University and Urban Wastewater by Shotgun Metagenomic Sequencing

  • Alessandra Nappo,
  • Adeel Mumtaz Abbasi,
  • Giulia Berno,
  • Martina Rueca and ,
  • Flavia Smoquina,
  • Cesare Ernesto Maria Gruber,
  • Lavinia Fabeni,
  • Pietro Giorgio Spezia,
  • Fabrizio Carletti and
  • Fabrizio Maggi
  • + 12 authors+ 7 authors

Wastewater-based surveillance has emerged as a powerful approach for population-level monitoring of pathogen circulation in a timely and non-invasive manner. In this study, shotgun metagenomic sequencing was applied to wastewater samples collected fr...

(This article belongs to the Special Issue The Microbiome: Bridging Human Health and Natural Ecosystems)
  • Article
  • Open Access
1,193 Views
22 Pages

Wastewater Surveillance: A National Concept for Germany—A Refined Approach to Surveillance Site Selection

  • Thomas Exner,
  • Ines Flügel,
  • Timo Greiner,
  • Marcus Lukas and ,
  • Nathan Obermaier,
  • Peter Pütz,
  • Cristina J. Saravia,
  • Alexander Schattschneider,
  • Antje Ullrich and
  • Ulrike Braun
  • + 5 authors+ 0 author

During the coronavirus disease-2019 (COVID-19) pandemic, wastewater-based surveillance (WBS) gained renewed importance by enabling nationwide assessment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) circulation independent of indivi...

(This article belongs to the Special Issue Pathogen Surveillance in Wastewater)
  • Article
  • Open Access
991 Views
16 Pages

19 February 2026

Wastewater-Based Epidemiology (WBE) has emerged as a critical tool for monitoring SARS-CoV-2 circulation at the community level. This study assessed spatiotemporal viral dynamics in Las Heras, Mendoza, Argentina, by comparing wastewater samples from...

(This article belongs to the Special Issue COVID and Public Health)
  • Article
  • Open Access
16 Citations
4,053 Views
13 Pages

SARS-CoV-2 Surveillance in Belgian Wastewaters

  • Raphael Janssens,
  • Sven Hanoteaux,
  • Hadrien Maloux,
  • Sofieke Klamer and ,
  • Valeska Laisnez,
  • Bavo Verhaegen,
  • Catherine Linard,
  • Lies Lahousse,
  • Peter Delputte and
  • Marie Lesenfants
  • + 11 authors+ 6 authors

2 September 2022

Wastewater-based surveillance was conducted by the national public health authority to monitor SARS-CoV-2 circulation in the Belgian population. Over 5 million inhabitants representing 45% of the Belgian population were monitored throughout 42 wastew...

(This article belongs to the Special Issue SARS-CoV-2 Research in Belgium)
  • Systematic Review
  • Open Access
5 Citations
1,856 Views
33 Pages

4 February 2026

Following the COVID-19 pandemic, researchers have increasingly focused on monitoring the spread of the virus and improving methods to detect changes in the SARS-CoV-2 genome. Although clinical surveillance provides direct and reliable results, it has...

(This article belongs to the Special Issue Wastewater-Based Epidemiology and Viral Surveillance)
  • Article
  • Open Access
10 Citations
5,019 Views
16 Pages

SARS-CoV-2 Monitoring in Wastewater Reveals Novel Variants and Biomarkers of Infection

  • Jenna McGowan,
  • Monica Borucki,
  • Hicham Omairi,
  • Merina Varghese and ,
  • Shahnaz Vellani,
  • Sukanya Chakravarty,
  • Shumin Fan,
  • Srestha Chattopadhyay,
  • Mashuk Siddiquee and
  • Saurabh Chattopadhyay
  • + 14 authors+ 9 authors

13 September 2022

Wastewater-based epidemiology (WBE) is a popular tool for the early indication of community spread of infectious diseases. WBE emerged as an effective tool during the COVID-19 pandemic and has provided meaningful information to minimize the spread of...

(This article belongs to the Special Issue Wastewater-Based Epidemiology (WBE) in COVID-19 Pandemics)
  • Article
  • Open Access
4 Citations
2,741 Views
10 Pages

Wastewater-based surveillance (WBS) on SARS-CoV-2 has been proved to be an effective approach to estimate the prevalence of COVID-19 in communities and cities. However, its application was overlooked at smaller scale, such as a single facility. Meat...

(This article belongs to the Special Issue Microbial Exposure Assessments in Different Occupational and Indoor Settings)
  • Article
  • Open Access
18 Citations
11,095 Views
24 Pages

Multifaceted Assessment of Wastewater-Based Epidemiology for SARS-CoV-2 in Selected Urban Communities in Davao City, Philippines: A Pilot Study

  • Maria Catherine B. Otero,
  • Lyre Anni E. Murao,
  • Mary Antoinette G. Limen,
  • Daniel Rev A. Caalim and ,
  • Paul Lorenzo A. Gaite,
  • Michael G. Bacus,
  • Joan T. Acaso,
  • Refeim M. Miguel,
  • Kahlil Corazo and
  • Dann Marie N. Del Mundo
  • + 10 authors+ 5 authors

Over 60 countries have integrated wastewater-based epidemiology (WBE) in their COVID-19 surveillance programs, focusing on wastewater treatment plants (WWTP). In this paper, we piloted the assessment of SARS-CoV-2 WBE as a complementary public health...

(This article belongs to the Special Issue COVID-19: Wastewater-Based Epidemiology)
  • Article
  • Open Access
1,318 Views
21 Pages

Wastewater-Based Estimation of COVID-19 Transmission in California: A Hierarchical Beta-Binomial Model for Estimating the Effective Reproduction Number

  • José Cricelio Montesinos-López,
  • Maria L. Daza-Torres,
  • Abelardo Montesinos-López,
  • Junlin Chen and ,
  • Heather N. Bischel and
  • Miriam Nuño
  • + 1 author+ 0 author

The coronavirus disease 2019 (COVID-19) pandemic highlighted the critical need for scalable, timely, and unbiased methods to monitor disease transmission at the population level. Wastewater-based epidemiology (WBE) provides an effective method for mo...

(This article belongs to the Special Issue Wastewater-Based Epidemiology Assessment and Surveillance)
  • Article
  • Open Access
370 Views
14 Pages

Pilot Monitoring of SARS-CoV-2 RNA in Influent and Treated Effluent of Municipal Wastewater Treatment Plants Across Slovakia During the Autumn 2020 COVID-19 Wave

  • Alexandra Tulipánová,
  • Zuzana Imreová,
  • Michal Tamáš,
  • Ján Krahulec and ,
  • Andrea Šoltýsová,
  • Andrej Ficek,
  • Marek Haššo,
  • Michal Hatala,
  • Jozef Ryba and
  • Tomáš Mackuľak
  • + 8 authors+ 3 authors

15 September 2026

Wastewater-based surveillance can track community SARS-CoV-2, but RNA signals at wastewater treatment plants are affected by sampling, analytical recovery and transfer to solids. We quantified SARS-CoV-2 RNA in influent and biologically treated efflu...

(This article belongs to the Section Water and One Health)
  • Article
  • Open Access
10 Citations
5,254 Views
18 Pages

Wastewater-based epidemiology (WBE) has great potential to monitor community public health, especially during pandemics. However, it faces substantial hurdles in pathogen surveillance through WBE, encompassing data representativeness, spatiotemporal...

(This article belongs to the Section Public Health Research)
  • Article
  • Open Access
4 Citations
2,104 Views
22 Pages

Wastewater Speaks: Evaluating SARS-CoV-2 Surveillance, Sampling Methods, and Seasonal Infection Trends on a University Campus

  • Shilpi Bhatia,
  • Tinyiko Nicole Maswanganye,
  • Olusola Jeje,
  • Danielle Winston and ,
  • Mehdi Lamssali,
  • Dongyang Deng,
  • Ivory Blakley,
  • Anthony A. Fodor and
  • Liesl Jeffers-Francis
  • + 4 authors+ 0 author

Wastewater surveillance has emerged as a cost-effective and equitable approach for tracking the spread of SARS-CoV-2. In this study, we monitored the prevalence of SARS-CoV-2 on a university campus over three years (2021–2023) using wastewater-...

(This article belongs to the Special Issue Surveillance of SARS-CoV-2 Employing Wastewater)
  • Article
  • Open Access
28 Citations
6,735 Views
16 Pages

8 November 2021

Essential components of public health include strengthening the surveillance of infectious diseases and developing early detection and prevention policies. This is particularly important for drug-resistant tuberculosis (DR-TB), which can be explored...

(This article belongs to the Special Issue Antibiotics and Antibiotic Resistance in Aquatic Environments)
  • Article
  • Open Access
4 Citations
3,275 Views
18 Pages

SARS-CoV-2 RNA Detection in Wastewater and Its Effective Correlation with Clinical Data during the Outbreak of COVID-19 in Salamanca

  • Ángel Emilio Martínez de Alba,
  • María Eugenia Morán-Diez,
  • Juan Carlos García-Prieto,
  • Juan García-Bernalt Diego and ,
  • Pedro Fernández-Soto,
  • Esteban Serrano León,
  • Víctor Monsalvo,
  • Marta Casao,
  • María Belén Rubio and
  • Enrique Monte
  • + 8 authors+ 3 authors

Wastewater treatment plants (WWTPs) are the final stage of the anthropogenic water cycle where a wide range of chemical and biological markers of human activity can be found. In COVID-19 disease contexts, wastewater surveillance has been used to infe...

(This article belongs to the Section Molecular Microbiology)
  • Article
  • Open Access
2 Citations
1,243 Views
12 Pages

Variabilities in N2 and E Gene Concentrations in a SARS-CoV-2 Wastewater Multiplex Assay

  • Ashley Green,
  • Aiswarya Rani Pappu,
  • Melanie Oakes,
  • Suzanne Sandmeyer and ,
  • Matthew Hileman and
  • Sunny Jiang
  • + 1 author+ 0 author

Wastewater can serve as both a source of pathogens that pose risks to human health and a valuable resource for tracking and predicting disease prevalence through wastewater-based surveillance (WBS). In WBS for SARS-CoV-2, both nucleocapsid-specific (...

(This article belongs to the Special Issue Water Microorganisms Associated with Human Health, 2nd Edition)
  • Article
  • Open Access
3 Citations
1,745 Views
13 Pages

Longitudinal Wastewater-Based Epidemiology Reveals the Spatiotemporal Dynamics and Genotype Diversity of Diarrheal Viruses in Urban Guangdong, China

  • Shuling Li,
  • Jiadian Cao,
  • Yuxi Yan,
  • Wenwen Deng and ,
  • Yuwei He,
  • Siling Xiang,
  • Chuting Zeng,
  • Heshi Long,
  • Shuxian Li and
  • Jing Lu
  • + 9 authors+ 4 authors

8 January 2026

Following the normalization of the COVID-19 pandemic, the focus of wastewater-based epidemiology (WBE) must be broadened from SARS-CoV-2 to encompass surveillance of other major infectious diseases, particularly for pathogens where conventional clini...

(This article belongs to the Special Issue Enteric Viruses in Environment and Humans: Identification, Surveillance and Control)
  • Article
  • Open Access
7 Citations
2,297 Views
19 Pages

Swift Realisation of Wastewater-Based SARS-CoV-2 Surveillance for Aircraft and Airports: Challenges from Sampling to Variant Detection

  • Cristina J. Saravia,
  • Kira Zachmann,
  • Natalie Marquar,
  • Ulrike Braun and ,
  • Claus Gerhard Bannick,
  • Timo Greiner,
  • Peter Pütz,
  • Susanne Lackner and
  • Shelesh Agrawal
  • + 4 authors+ 0 author

International air traffic has contributed to the global spread of SARS-CoV-2 and its variants. In early 2023, wastewater-based epidemiology (WBE) has been implemented at airports as a surveillance tool to detect emerging variants at short notice. Thi...

(This article belongs to the Special Issue Surveillance of SARS-CoV-2 Employing Wastewater)
  • Article
  • Open Access
3 Citations
3,035 Views
16 Pages

Wastewater Surveillance of SARS-CoV-2 in Slovenia: Key Public Health Tool in Endemic Time of COVID-19

  • Natalija Kranjec,
  • Andrej Steyer,
  • Tjaša Cerar Kišek,
  • Tom Koritnik and ,
  • Tea Janko,
  • Maja Bolješić,
  • Vid Vedlin,
  • Verica Mioč,
  • Barbara Lasecky and
  • An Galičič
  • + 10 authors+ 5 authors

With the reclassification of COVID-19 as an endemic disease and the relaxation of measures, Slovenia needed a complementary system for monitoring SARS-CoV-2 infections. This article provides an overview of the epidemiological situation of SARS-CoV-2...

(This article belongs to the Special Issue Surveillance of SARS-CoV-2 Employing Wastewater)
  • Article
  • Open Access
207 Views
37 Pages

First Longitudinal Untargeted Metagenomic Survey Reveals Spatial and Temporal Structuring of the Microbial Community in Selected Tunisian Wastewater Treatment Plants

  • Fatma Zahra Guerfali,
  • Chiraz Atri,
  • Azimdine Habib,
  • Aymen Bali and ,
  • Elodie Turc,
  • Yousra Akrichi,
  • Lilia Malouche,
  • Inès Brahmi,
  • Hana Trigui and
  • Dhafer Laouini
  • + 10 authors+ 5 authors

24 October 2026

Wastewater-based metagenomics offers a comprehensive, population-level, and unbiased window into circulating microorganisms, complementing clinical surveillance by capturing signals across an entire connected population rather than only individuals s...

(This article belongs to the Special Issue Integrated Surveillance of Pathogens with Pandemic and/or Epidemic Potential)

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