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20 pages, 4886 KB  
Article
Spatiotemporal Variation and Driving Mechanisms of Land Surface Temperature in the Urumqi Metropolitan Area Based on Land Use Change
by Buwajiaergu Shayiti and Alimujiang Kasimu
Land 2025, 14(11), 2252; https://doi.org/10.3390/land14112252 (registering DOI) - 13 Nov 2025
Abstract
Land use change is closely related to land surface temperature (LST). Based on remote sensing data from 2001 to 2020, this study analyzed the spatiotemporal variations and driving mechanisms of daytime and nighttime LST in the Urumqi Metropolitan Area (UMA) by combining traditional [...] Read more.
Land use change is closely related to land surface temperature (LST). Based on remote sensing data from 2001 to 2020, this study analyzed the spatiotemporal variations and driving mechanisms of daytime and nighttime LST in the Urumqi Metropolitan Area (UMA) by combining traditional methods with the eXtreme Gradient Boosting (XGBoost)–SHAP coupled model. Although the average LST trend in the region was one of warming, the pixel-level significance analysis indicated that statistically significant warming (p < 0.05) is concentrated mainly in the urban core (2.65% of the area), while the majority of the region (70%) showed a non-significant warming trend. LST displayed significant spatial clustering, with Moran’s I remaining above 0.990, indicating a positive spatial autocorrelation in spatial distribution. With the advancement of urbanization, the proportion of impervious surfaces increased from 0.87% to 1.14%, while wastelands consistently accounted for approximately 50% of the total area. Different land use types showed distinct effects on the urban heat island (UHI) phenomenon: water bodies, grasslands, and forests played cooling roles, whereas barren land and impervious areas were the main heat contributors. The XGBoost-SHAP analysis further revealed that the importance ranking of driving factors has evolved over time. Among these factors, Elevation dominates, while the influence of population-related factors increased significantly in 2020. This study provides a scientific basis for regulating the thermal environment of cities in arid regions from the perspective of land use. This study provides a scientific basis for regulating the thermal environment of arid-region cities from the perspective of land use. Full article
(This article belongs to the Section Land Innovations – Data and Machine Learning)
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32 pages, 5540 KB  
Review
Silk Fibroin-Derived Smart Living Hydrogels for Regenerative Medicine and Organoid Engineering: Bioactive, Adaptive, and Clinically Translatable Platforms
by Asim Mushtaq, Khai Ly Do, Abdul Wahab, Muhammad Yousaf, Abdul Rahman, Hamid Hussain, Muhammad Ali, Pingfan Du and Miao Su
Gels 2025, 11(11), 908; https://doi.org/10.3390/gels11110908 (registering DOI) - 13 Nov 2025
Abstract
Silk fibroin (SF) has evolved from a traditional biopolymer to a leading regenerative medicine material. Its combination of mechanical strength, biocompatibility, tunable degradation, and molecular adaptability makes SF a unique matrix that is both bioactive and intelligent. Advances in hydrogel engineering have transformed [...] Read more.
Silk fibroin (SF) has evolved from a traditional biopolymer to a leading regenerative medicine material. Its combination of mechanical strength, biocompatibility, tunable degradation, and molecular adaptability makes SF a unique matrix that is both bioactive and intelligent. Advances in hydrogel engineering have transformed SF from a passive scaffold into a smart, living hydrogel. These systems can instruct cell fate, sense microenvironmental signals, and deliver therapeutic signals as needed. By incorporating stem cells, progenitors, or engineered immune and microbial populations, SF hydrogels now serve as synthetic niches for organoid maturation and as adaptive implants for tissue regeneration. These platforms replicate extracellular matrix complexity and evolve with tissue, showing self-healing, shape-memory, and stimuli-responsive properties. Such features are redefining biomaterial–cell interactions. SF hydrogels are used for wound healing, musculoskeletal repair, neural and cardiac patches, and developing scalable organoid models for disease and drug research. Challenges remain in maintaining long-term cell viability, achieving clinical scalability, and meeting regulatory standards. This review explores how advances in SF engineering, synthetic biology, and organoid science are enabling SF-based smart living hydrogels in bridging the gap between research and clinical use. Full article
(This article belongs to the Special Issue Hydrogel-Based Scaffolds with a Focus on Medical Use (3rd Edition))
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20 pages, 7877 KB  
Article
Draft Genome Assembly of Parnassius epaphus Provides New Insights into Transposable Elements That Drive Genome Expansion in Alpine Parnassius butterflies
by Wantao Rong, Nan Wei, Jing Song, Guole Qin and Delong Guan
Diversity 2025, 17(11), 794; https://doi.org/10.3390/d17110794 (registering DOI) - 13 Nov 2025
Abstract
The expansion of genomes is a major evolutionary force, yet its role in facilitating adaptation to extreme environments remains enigmatic. Here, we investigate alpine Parnassius butterflies, a rare genus characterized by exceptionally large genomes, to unravel the interplay between genome architecture and [...] Read more.
The expansion of genomes is a major evolutionary force, yet its role in facilitating adaptation to extreme environments remains enigmatic. Here, we investigate alpine Parnassius butterflies, a rare genus characterized by exceptionally large genomes, to unravel the interplay between genome architecture and high-altitude colonization. We present a new, 1.46 Gb draft genome assembly for Parnassius epaphus and perform a comparative analysis across six species. Our findings reveal a massive 3- to 5-fold genome expansion driven predominantly by Long Interspersed Nuclear Elements (LINEs). Counterintuitively, we discover that larger genomes possess a proportionally smaller fraction of young, active transposable elements (TEs), challenging the prevailing paradigm that recent TE proliferation is the primary driver of genome size. Instead, our temporal analysis demonstrates that this expansion is a legacy of two ancient TE waves (~8 and ~14 Mya), which remarkably coincide with major uplift phases of the Tibetan Plateau. We propose a model where the selective retention of these ancient TEs, mechanistically linked to major geological upheavals, provided the crucial genomic plasticity for colonizing Earth’s most extreme terrestrial habitats. This study re-frames TEs not merely as genomic parasites but as pivotal architects of adaptive genome evolution in response to profound environmental change. Full article
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14 pages, 3122 KB  
Article
Environmentally Friendly Silk Fibroin/Polyethyleneimine High-Performance Triboelectric Nanogenerator for Energy Harvesting and Self-Powered Sensing
by Ziyi Guo, Xinrong Xu, Yue Shen, Menglong Wang, Youzhuo Zhai, Haiyan Zheng and Jiqiang Cao
Coatings 2025, 15(11), 1323; https://doi.org/10.3390/coatings15111323 - 12 Nov 2025
Abstract
Due to the large emissions of greenhouse gases from the burning of fossil fuels and people’s demand for green materials and energy, the development of environmentally friendly triboelectric nanogenerators (TENGs) is becoming increasingly significant. Silk fibroin (SF) is considered an ideal biopolymer candidate [...] Read more.
Due to the large emissions of greenhouse gases from the burning of fossil fuels and people’s demand for green materials and energy, the development of environmentally friendly triboelectric nanogenerators (TENGs) is becoming increasingly significant. Silk fibroin (SF) is considered an ideal biopolymer candidate for fabricating green TENGs due to its biodegradability and renewability. However, its intrinsic brittleness and relatively weak triboelectric performance severely limit its practical applications. In this study, SF was physically blended with poly(ethylenimine) (PEI), a polymer rich in amino groups, to fabricate SF/PEI composite films. The resulting films were employed as tribopositive layers and paired with a poly(tetrafluoroethylene) (PTFE) tribonegative layer to assemble high-performance TENGs. Experimental results revealed that the incorporation of PEI markedly enhanced the flexibility and electron-donating capability of composite films. By optimizing the material composition, the SF/PEI-based TENG achieved an open-circuit voltage as high as 275 V and a short-circuit current of 850 nA, with a maximum output power density of 13.68 μW/cm2. Application tests demonstrated that the device could serve as an efficient self-powered energy source, capable of lighting up 66 LEDs effortlessly through simple hand tapping and driving small electronic components such as timers. In addition, the device can function as a highly sensitive self-powered sensor, capable of generating rapid and distinguishable electrical responses to various human motions. This work not only provides an effective strategy to overcome the intrinsic limitations of SF-based materials but also opens up new avenues for the development of high-performance and environmentally friendly technologies for energy harvesting and sensing. Full article
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13 pages, 3146 KB  
Article
Improved Polymer Membrane for Textile Zinc-Ion Capacitor
by Sheng Yong, Sasikumar Arumugam and Stephen Paul Beeby
Polymers 2025, 17(22), 2995; https://doi.org/10.3390/polym17222995 - 11 Nov 2025
Abstract
This work presents the design, fabrication and characterisation of an improved textile energy storage device implemented in a single layer of polyester cotton and silk fabric. To achieve this, the energy storage device has evolved from an electrical double-layer (EDL) supercapacitor to a [...] Read more.
This work presents the design, fabrication and characterisation of an improved textile energy storage device implemented in a single layer of polyester cotton and silk fabric. To achieve this, the energy storage device has evolved from an electrical double-layer (EDL) supercapacitor to a zinc-ion supercapacitor (ZHSC) with an optimised co-polymer membrane containing a polyethene oxide (PEO) additive and a polyvinylidene (PVDF)-based organic electrolyte. The flexible textile ZHSC achieved an areal capacitance of 159.5 mF cm−2 and an energy density of 52.3 µWh cm−2 (increasing by a factor of 4 and 1.8, respectively, on the previous work) with a power density of 0.27 mW cm−2 and good bending stability. Full article
(This article belongs to the Special Issue Polymeric Materials for Next-Generation Energy Storage)
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15 pages, 5245 KB  
Article
Development and Evaluation of Yarns Made from Mechanically Recycled Textiles
by Kendall Ludwig, Sophia Gupman, Michelle Yatvitskiy, Huantian Cao and Kelly Cobb
Textiles 2025, 5(4), 56; https://doi.org/10.3390/textiles5040056 - 11 Nov 2025
Abstract
Mechanical textile recycling presents a sustainable alternative to linear “take–make–waste” models in the fashion industry. This study intended to develop yarns using textile-to-fiber mechanically recycled fibers. ReSpool mechanically recycled wool, cotton, polyester, silk, and rayon fibers from pre-consumer and post-consumer textiles were acquired [...] Read more.
Mechanical textile recycling presents a sustainable alternative to linear “take–make–waste” models in the fashion industry. This study intended to develop yarns using textile-to-fiber mechanically recycled fibers. ReSpool mechanically recycled wool, cotton, polyester, silk, and rayon fibers from pre-consumer and post-consumer textiles were acquired and blended with new fibers at varying ratios (100% ReSpool fibers, 85% ReSpool fibers, and 65% ReSpool fibers) to make batts, which were spun into yarns. The yarns’ size (Tex), strength (breaking force and tenacity), elongation, and moisture regain were evaluated. ReSpool recycled fibers from both pre-consumer and post-consumer textiles can be used to produce yarns that have appropriate strength for weaving and knitting. It was possible to produce yarns from 100% ReSpool recycled wool, polyester, and silk fibers, but ReSpool recycled cotton and rayon fibers must be blended with new fibers to produce yarns. There was no significant difference among the percentage of ReSpool recycled polyester and cotton fibers in the yarns on the strength and elongation of the yarn. It is recommended to use the higher percentage of ReSpool recycled fibers in yarn development to maximize recycled material utilization. Full article
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24 pages, 1232 KB  
Review
Frugal Innovation and Patent Analysis in Sericulture: Lessons for Sustainable Rural Bioeconomy Systems
by Mónica Fernanda Suárez-Sánchez, Humberto Merritt, Carlos Victor Muñoz-Ruiz, Mauricio Suárez-Sánchez, Ernesto Oregel-Zamudio and Sergio Arias-Martínez
Sustainability 2025, 17(22), 10026; https://doi.org/10.3390/su172210026 - 10 Nov 2025
Viewed by 289
Abstract
Sericulture sustains rural livelihoods in Asia, Africa, and Latin America, where it provides income for women, elderly workers, and smallholder households. Yet this sector faces a critical technological divide: traditional reeling methods remain labor-intensive and uncompetitive, while industrial innovations advance along trajectories that [...] Read more.
Sericulture sustains rural livelihoods in Asia, Africa, and Latin America, where it provides income for women, elderly workers, and smallholder households. Yet this sector faces a critical technological divide: traditional reeling methods remain labor-intensive and uncompetitive, while industrial innovations advance along trajectories that are poorly suited to low-resource contexts. This article presents a patent landscape of silk-reeling technologies retrieved from Espacenet and PATENTSCOPE (2000–2024), comprising 212 unique records. Patents were evaluated against six criteria: resource efficiency, knowledge accessibility, durability and reparability, context adaptability, equity and inclusion, and by-product valorization. This review reveals a strong industrial bias, with most patents emphasizing energy-intensive steaming, mechanized feeding, and digital control, while only a small fraction addresses rural conditions or social inclusion. Current innovations therefore tend to marginalize traditional producers from emerging bio-based value chains. This study contributes to discussions on how technological design can support rural sericulture, highlighting the need for resource-efficient, modular, and socially inclusive solutions. Future research should extend patent analysis to mulberry cultivation, silkworm breeding, and by-product recovery to fully integrate sericulture into the circular bioeconomy. Full article
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29 pages, 2408 KB  
Article
Can Biodiversity Disclosure Improve Stock Liquidity? Evidence from China
by Haonan Lin, Yongliang Yang and Mengmeng Qiang
Sustainability 2025, 17(22), 9950; https://doi.org/10.3390/su17229950 - 7 Nov 2025
Viewed by 400
Abstract
Biodiversity loss poses a threat to corporate performance and social welfare. Biodiversity disclosure enables investors to evaluate firms’ biodiversity status. However, it remains unclear whether and how biodiversity disclosure affects capital market efficiency. In this paper, we employ a binary variable derived from [...] Read more.
Biodiversity loss poses a threat to corporate performance and social welfare. Biodiversity disclosure enables investors to evaluate firms’ biodiversity status. However, it remains unclear whether and how biodiversity disclosure affects capital market efficiency. In this paper, we employ a binary variable derived from a word-frequency analysis of annual reports to determine whether a firm has disclosed biodiversity information. Using a panel of Chinese listed companies from 2011 to 2022, we provide robust evidence that Companies that disclose biodiversity information have experienced sustained improvements in stock liquidity. Furthermore, the effect is significantly amplified after the 2020 UN Biodiversity Summit, suggesting that investors respond positively to biodiversity disclosure. Channel analysis reveals that higher inventory turnover reinforces this positive effect, while greater financing constraints and higher management ownership weaken it. Heterogeneity analysis further indicates that this effect is more pronounced among firms with higher environmental information asymmetry, lower supply chain transparency, and lower patient capital. This study sheds light on how biodiversity disclosure affects market efficiency and offers important insights for future research and policy. Full article
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18 pages, 2286 KB  
Article
Design and Characterization of Cosmetic Creams Based on Natural Oils from the Rosaceae Family
by Katya Hutova, Velichka Andonova, Natalina Panova, Ivan Ivanov, Krastena Nikolova and Viliana Gugleva
Cosmetics 2025, 12(6), 248; https://doi.org/10.3390/cosmetics12060248 - 6 Nov 2025
Viewed by 238
Abstract
Background: Creams are one of the most used cosmetic formulations due to their hydrating and restoring properties, pleasant texture, and the possibility to deliver hydrophobic and hydrophilic active ingredients. The study aims to develop and characterize cosmetic creams based on natural-sourced ingredients—different vegetable [...] Read more.
Background: Creams are one of the most used cosmetic formulations due to their hydrating and restoring properties, pleasant texture, and the possibility to deliver hydrophobic and hydrophilic active ingredients. The study aims to develop and characterize cosmetic creams based on natural-sourced ingredients—different vegetable oils from the Rosacea family: (1) Chaenomelis japonica seed oil, (2) Rosa canina seed oil, (3) Prunus armeniaca kernel oil, alone and in combination (4), along with silk protein (sericin) and astaxanthin. Methods: The creams were prepared by the hot homogenization method and were characterized in terms of rheological properties, spreadability, and antioxidant activity. Results: Two suitable formulations were selected as feasible for cosmetic application—the model containing Chaenomelis japonica seed oil exhibiting the highest antioxidant activity (47% higher vs. the model based on rosehip oil by FRAP method) and the highest spreadability values among the tested formulations (ranging between 61.57 and 69.34 mm). The second selected optimal formulation is the model based on a combination of oils characterized by the most suitable consistency and high antioxidant activity. Conclusions: The differences in their rheological behavior suggest as feasible application of Japanese quince oil cream its use as a daycare antioxidant cream, whereas the mixed oils-based cream model may potentially serve as a night cream for restorative and antioxidant care. Full article
(This article belongs to the Section Cosmetic Formulations)
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17 pages, 6748 KB  
Article
Referenced Transcriptomics Identifies a Core Set of Cytochrome P450 Genes Driving Broad-Spectrum Insecticide Detoxification in Phthonandria atrilineata
by Delong Guan, Jing Song, Yue Qin, Lei Xin, Xiaodong Li and Shihao Zhang
Agronomy 2025, 15(11), 2561; https://doi.org/10.3390/agronomy15112561 - 5 Nov 2025
Viewed by 307
Abstract
Phthonandria atrilineata, also known as the mulberry looper, is a major defoliator of mulberry trees. This feeding behavior directly affects the growth of the trees and reduces the quality and yield of mulberry leaves for its use in sericulture. Despite its importance [...] Read more.
Phthonandria atrilineata, also known as the mulberry looper, is a major defoliator of mulberry trees. This feeding behavior directly affects the growth of the trees and reduces the quality and yield of mulberry leaves for its use in sericulture. Despite its importance the molecular basis of its resistance to insecticides remains poorly understood. Therefore, this study aimed to comprehensively characterize the cytochrome P450 monooxygenases (P450s) gene family in P. atrilineata and identify key effectors responsible for responses to diverse chemical stressors. We integrated genome-wide re-annotation, phylogenetic analysis, and comparative transcriptomics following exposure to five chemically distinct insecticides. We identified a high-confidence set of 70 P450 genes, dominated by the CYP6 and CYP4 families, whose expansion was driven by tandem gene duplication. Transcriptomic analysis revealed a powerful yet highly selective “elite-driven” response, wherein a small subset of P450s was strongly induced by multiple insecticides. Random Forest and Support Vector Machine (SVM) models converged with differential expression data to pinpoint a core trio of P450s as primary drivers of detoxification: two generalists, CYP6(09521) and CYP6(04876), responsive to all compounds, and one potent specialist, CYP4(04803), exhibiting massive induction to a specific subset of insecticides. Our findings uncover a complex, energy-efficient metabolic strategy in P. atrilineata and identify pivotal P450 genes for broad-spectrum detoxification. These genes represent high-priority targets for developing molecular diagnostic tools for resistance monitoring and informing scientifically guided insecticide rotation strategies. Full article
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16 pages, 1929 KB  
Article
Influence of Meteorological Factors and Sowing Dates on Growth and Yield Traits of Summer Maize in Northeastern Sichuan, China
by Yun Long, Yun Yang, Chuan He and Xiaohong Liu
Agriculture 2025, 15(21), 2294; https://doi.org/10.3390/agriculture15212294 - 3 Nov 2025
Viewed by 313
Abstract
This study investigates meteorological factors’ effects on summer maize growth, agronomic traits and yield in northeastern Sichuan, China, under different sowing dates. A five-gradient sowing date experiment was conducted with three varieties from 2023 to 2024. The results showed delayed sowing prolonged total [...] Read more.
This study investigates meteorological factors’ effects on summer maize growth, agronomic traits and yield in northeastern Sichuan, China, under different sowing dates. A five-gradient sowing date experiment was conducted with three varieties from 2023 to 2024. The results showed delayed sowing prolonged total growth period mainly in the joint–tasseling and silking–maturity stages. Early sowing (5th May and 20th May) significantly improved key agronomic traits and increased grain yield, with Xianyu 1171 achieving the highest yield of 9.77 t ha−1 under early sowing. Meteorological factors had limited influence during vegetative growth but strongly affected reproductive growth. Among them, average temperature (AT) and growing degree days (GDDs) were critical throughout the growth cycle, though their effects varied by stage. These findings suggest that adjusting sowing dates to align key growth stages with favorable weather—particularly by avoiding high-temperature stress during flowering and ensuring sufficient warmth during grain filling—can enhance yield stability. This study provides a basis for constructing a climate-resilient cultivation system and promoting stable and high summer maize yields in the hilly areas of northeastern Sichuan. Full article
(This article belongs to the Special Issue Effect of Cultivation Practices on Crop Yield and Quality)
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19 pages, 3033 KB  
Article
Optimizing Nitrogen Fertilization in Maize Production to Improve Yield and Grain Composition Based on NDVI Vegetation Assessment
by Árpád Illés, Csaba Bojtor, Endre Harsányi, János Nagy, Lehel Lengyel and Adrienn Széles
Agriculture 2025, 15(21), 2279; https://doi.org/10.3390/agriculture15212279 - 31 Oct 2025
Viewed by 347
Abstract
Nitrogen fertilization is essential for balancing maize yield, grain composition, and environmental sustainability. This study aimed to evaluate the relationship between nitrogen (N) supply, grain quality traits, and yield potential using UAV-based Normalized Difference Vegetation Index (NDVI) monitoring in a long-term fertilization field [...] Read more.
Nitrogen fertilization is essential for balancing maize yield, grain composition, and environmental sustainability. This study aimed to evaluate the relationship between nitrogen (N) supply, grain quality traits, and yield potential using UAV-based Normalized Difference Vegetation Index (NDVI) monitoring in a long-term fertilization field experiment in Eastern Hungary. Six N levels (0–300 kg ha−1) were tested during two consecutive growing seasons (2023–2024) under varying climatic conditions. The obtained results showed that moderate N doses (120–180 kg ha−1) provided the optimal nutrition level for maize, significantly increasing yield compared to the control (+5.086 t ha−1 in 2024), while excessive fertilization above 180 kg ha−1 did not result in any substantial yield gains; however, it significantly modified grain composition. Higher N supply enhanced protein content (+0.95% between 0 and 300 kg ha−1) and reduced starch percentage, confirming the protein–starch trade-off, whereas oil content was less affected by nitrogen fertilization, similarly to previous results. The strongest correlation between NDVI values and yield was measured at the post-silking stage (112 DAS; R = 0.638 in 2023, R = 0.634 in 2024), indicating the suitability of NDVI monitoring for in-season yield prediction. Overall, NDVI-based monitoring proved effective not just for optimizing N management but also for supporting site specific fertilization strategies to enhance maize productivity and nutrient use efficiency. Full article
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21 pages, 2324 KB  
Article
Analysis of Spatio-Temporal Development Patterns in Key Port Cities Along the Belt and Road Using Nighttime Light Data
by Ronglei Yang, Tiyan Shen, Weiwei Cao, Jidong Zhang and Shuai Jiang
Mathematics 2025, 13(21), 3477; https://doi.org/10.3390/math13213477 - 31 Oct 2025
Viewed by 218
Abstract
The Belt and Road Initiative (BRI) has reshaped global trade and infrastructure, with port cities as key nodes in its Maritime Silk Road. Quantifying their spatiotemporal development is challenging due to data limitations in emerging economies. This study employs VIIRS nighttime light (NTL) [...] Read more.
The Belt and Road Initiative (BRI) has reshaped global trade and infrastructure, with port cities as key nodes in its Maritime Silk Road. Quantifying their spatiotemporal development is challenging due to data limitations in emerging economies. This study employs VIIRS nighttime light (NTL) data from 2013 to 2023 to analyze urbanization patterns in twelve BRI port cities spanning Asia, Africa, Europe, and South America. We compile a 12-city cohort; inferential analyses are conducted for a pre-specified six-city subset, while descriptive NTL trends cover all 12. This study makes three contributions: (i) we assemble a cross-sensor harmonized VIIRS NTL record for 12 BRI port cities during 2013–2023; (ii) we integrate Standard Deviational Ellipse(SDE) parameters with rank-size dynamics as a joint diagnostic of urban hierarchy; and (iii) we triangulate NTL with external indicators (GDP, population, port throughput) to validate interpretation. Three key findings emerge: Asian ports experienced pronounced NTL growth, with Singapore approaching saturation, consistent with the luminosity-ceiling hypothesis; SDE analysis shows varied expansion patterns shaped by geophysical and policy factors; and rank-size trends indicate decentralization during the BRI decade, with |q| declining in most cities, challenging the primate-city model. To optimize development, we highlight polycentric infrastructure investment, institutionalized NTL monitoring, and green port certification aligned with sustainability goals. Full article
(This article belongs to the Special Issue Spatial Statistics: Methods and Applications)
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12 pages, 1242 KB  
Article
Long-Term Stabilization of Dengue Virus RNA at 37 °C for 14 Months Using Silk Fibroin Films
by Nuo Wang, Ping He, Bohan Xu, Hongping Wei and Junping Yu
Viruses 2025, 17(11), 1452; https://doi.org/10.3390/v17111452 - 31 Oct 2025
Viewed by 258
Abstract
Diagnosis of dengue virus infections typically relies on RT-PCR-based methods, for which reliable positive controls are essential. Viral RNA is an ideal positive control, but its inherent instability poses a major challenge. Herein, we report a simple and effective method for stabilizing dengue [...] Read more.
Diagnosis of dengue virus infections typically relies on RT-PCR-based methods, for which reliable positive controls are essential. Viral RNA is an ideal positive control, but its inherent instability poses a major challenge. Herein, we report a simple and effective method for stabilizing dengue virus RNA by immobilizing it onto silk fibroin films (RNA-SFFs). We evaluated various substrate surfaces for RNA-SFFs preparation and found that the inner surface of sealable bags is optimal for uniform film formation and easy harvesting. Screening different silk fibroin concentrations revealed that even low concentrations (2.8%) effectively preserved RNA well and kept Ct constant for up to 16 days at 25 °C, 37 °C, and even 45 °C (extreme weather for transportations). Due to its rapid film formation and ease of peeling, 7% silk fibroin was selected. Notably, the RNA-SFFs demonstrated robust resistance to UV irradiation, with no significant Ct value changes after 4 h of exposure. Long-term stability testing at −20 °C, 25 °C, and 37 °C showed that dengue serotype 1–4 RNA-SFFs remained stable for the entire duration of the study—up to 56 weeks (approximately 14 months)—at all tested temperatures. These results demonstrate that RNA-SFFs are highly stable, portable, and practical as positive controls for dengue diagnostics, with strong potential for use in on-site and resource-limited settings. Full article
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14 pages, 924 KB  
Article
Effects of Mulberry Leaf and Corn Silk Extracts Against α-Amylase and α-Glucosidase In Vitro and on Postprandial Glucose in Prediabetic Individuals: A Randomized Crossover Trial
by You Sun, Xiaokang Niu, Yifan Wang, Qi Zhang, Yan Liu, Jingjing He, Lingling Xu, Ran Wang and Jie Guo
Nutrients 2025, 17(21), 3438; https://doi.org/10.3390/nu17213438 - 31 Oct 2025
Viewed by 401
Abstract
Objective: Postprandial hyperglycemia is a major risk factor for type 2 diabetes and cardiovascular disease. Inhibition of α-amylase and α-glucosidase can attenuate postprandial glycemic response (PPGR). This study aimed to investigate the inhibitory effects of mulberry leaf and corn silk on these enzymes [...] Read more.
Objective: Postprandial hyperglycemia is a major risk factor for type 2 diabetes and cardiovascular disease. Inhibition of α-amylase and α-glucosidase can attenuate postprandial glycemic response (PPGR). This study aimed to investigate the inhibitory effects of mulberry leaf and corn silk on these enzymes in vitro and their impact on postprandial glucose (PG) levels in prediabetic individuals using milk-based matrices. Research Design and Methods: In vitro, enzyme inhibition was assessed using the DNS method (α-amylase) and pNPG method (α-glucosidase). A randomized crossover trial was conducted in 11 prediabetic individuals with four interventions: pure milk; lactose-hydrolyzed milk; lactose-hydrolyzed milk with mulberry leaf, corn silk, and resistant dextrin; and GOS milk with mulberry leaf and corn silk. PPGR was assessed by area under the glucose curve, 1 and 2 h PG, maximum PG, and 2 h glucose excursion. Paired Wilcoxon signed-rank tests were used for comparisons. Results: Mulberry leaf and corn silk extracts inhibited both enzymes dose-dependently, with synergistic effects. No significant differences in PPGR indices were observed across interventions in the overall prediabetic individuals. However, in the overweight subgroup, the combination of GOS milk supplemented with mulberry leaf and corn silk significantly reduced 1 h PG (median difference [P25, P75]: −0.84 mmol/L [−1.05, −0.49]), maximum PG (−0.54 mmol/L [−0.75, −0.25]), and glucose excursion (−0.62 mmol/L [−0.75, −0.24]) compared to pure milk. Conclusions: Mulberry leaf and corn silk extracts inhibit α-amylase and α-glucosidase in vitro and may attenuate postprandial glucose excursions in overweight prediabetic individuals when delivered in a GOS milk matrix. Full article
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