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15 pages, 1444 KiB  
Article
Touchscreen Tasks for Cognitive Testing in Domestic Goats (Capra hircus): A Pilot Study Using Odd-Item Search Training
by Jie Gao, Yumi Yamanashi and Masayuki Tanaka
Animals 2025, 15(14), 2115; https://doi.org/10.3390/ani15142115 (registering DOI) - 17 Jul 2025
Abstract
The cognition of large farm animals is important for understanding how cognitive abilities are shaped by evolution and domestication. Valid testing methods are needed with the development of cognitive studies in more species. Here, a step-by-step method for training four naïve domestic goats [...] Read more.
The cognition of large farm animals is important for understanding how cognitive abilities are shaped by evolution and domestication. Valid testing methods are needed with the development of cognitive studies in more species. Here, a step-by-step method for training four naïve domestic goats to use a touchscreen in cognitive tests is described. The goats made accurate touches smoothly after training. Follow-up tests were conducted to confirm that they could do cognitive tests on a touchscreen. In the pilot test of odd-item search, all the goats had above-chance level performances in some conditions. In the subsequent odd-item search tasks using multiple novel stimulus sets, one goat could achieve the criterion and complete several stages, and the results showed a learning effect. These suggest a potential ability to learn the rule of odd-item search. Not all goats could pass the criteria, and there were failures in the transfer, indicating a perceptual strategy rather than using the odd-item search rule. The experiment confirmed that goats could use the touchscreen testing system for cognitive tasks and demonstrated their approaches in tackling this problem. We also hope that these training methods will help future studies training and testing naïve animals. Full article
(This article belongs to the Section Animal System and Management)
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22 pages, 761 KiB  
Review
Insights from Mass Spectrometry-Based Proteomics on Cryptococcus neoformans
by Jovany Jordan Betancourt and Kirsten Nielsen
J. Fungi 2025, 11(7), 529; https://doi.org/10.3390/jof11070529 (registering DOI) - 17 Jul 2025
Abstract
Cryptococcus neoformans is an opportunistic fungal pathogen and causative agent of cryptococcosis and cryptococcal meningitis (CM). Cryptococcal disease accounts for up to 19% of AIDS-related mortalities globally, warranting its label as a pathogen of critical priority by the World Health Organization. Standard treatments [...] Read more.
Cryptococcus neoformans is an opportunistic fungal pathogen and causative agent of cryptococcosis and cryptococcal meningitis (CM). Cryptococcal disease accounts for up to 19% of AIDS-related mortalities globally, warranting its label as a pathogen of critical priority by the World Health Organization. Standard treatments for CM rely heavily on high doses of antifungal agents for long periods of time, contributing to the growing issue of antifungal resistance. Moreover, mortality rates for CM are still incredibly high (13–78%). Attempts to create new and effective treatments have been slow due to the complex and diverse set of immune-evasive and survival-enhancing virulence factors that C. neoformans employs. To bolster the development of better clinical tools, deeper study into host–Cryptococcus proteomes is needed to identify clinically relevant proteins, pathways, antigens, and beneficial host response mechanisms. Mass spectrometry-based proteomics approaches serve as invaluable tools for investigating these complex questions. Here, we discuss some of the insights into cryptococcal disease and biology learned using proteomics, including target proteins and pathways regulating Cryptococcus virulence factors, metabolism, and host defense responses. By utilizing proteomics to probe deeper into these protein interaction networks, new clinical tools for detecting, diagnosing, and treating C. neoformans can be developed. Full article
(This article belongs to the Special Issue Proteomic Studies of Pathogenic Fungi and Hosts)
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18 pages, 871 KiB  
Review
Artificial Intelligence-Assisted Selection Strategies in Sheep: Linking Reproductive Traits with Behavioral Indicators
by Ebru Emsen, Muzeyyen Kutluca Korkmaz and Bahadir Baran Odevci
Animals 2025, 15(14), 2110; https://doi.org/10.3390/ani15142110 (registering DOI) - 17 Jul 2025
Abstract
Reproductive efficiency is a critical determinant of productivity and profitability in sheep farming. Traditional selection methods have largely relied on phenotypic traits and historical reproductive records, which are often limited by subjectivity and delayed feedback. Recent advancements in artificial intelligence (AI), including video [...] Read more.
Reproductive efficiency is a critical determinant of productivity and profitability in sheep farming. Traditional selection methods have largely relied on phenotypic traits and historical reproductive records, which are often limited by subjectivity and delayed feedback. Recent advancements in artificial intelligence (AI), including video tracking, wearable sensors, and machine learning (ML) algorithms, offer new opportunities to identify behavior-based indicators linked to key reproductive traits such as estrus, lambing, and maternal behavior. This review synthesizes the current research on AI-powered behavioral monitoring tools and proposes a conceptual model, ReproBehaviorNet, that maps age- and sex-specific behaviors to biological processes and AI applications, supporting real-time decision-making in both intensive and semi-intensive systems. The integration of accelerometers, GPS systems, and computer vision models enables continuous, non-invasive monitoring, leading to earlier detection of reproductive events and greater breeding precision. However, the implementation of such technologies also presents challenges, including the need for high-quality data, a costly infrastructure, and technical expertise that may limit access for small-scale producers. Despite these barriers, AI-assisted behavioral phenotyping has the potential to improve genetic progress, animal welfare, and sustainability. Interdisciplinary collaboration and responsible innovation are essential to ensure the equitable and effective adoption of these technologies in diverse farming contexts. Full article
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488 KiB  
Proceeding Paper
Digital Twins for Circular Economy Optimization: A Framework for Sustainable Engineering Systems
by Shubham Gupta
Proceedings 2025, 121(1), 4; https://doi.org/10.3390/proceedings2025121004 (registering DOI) - 16 Jul 2025
Abstract
This paper introduces sustainable engineering systems built using digital twin technology and circular economy principles. This research presents a framework for monitoring, modeling, and making decisions in real timusing virtual replicas of physical products, processes, and systems in product lifecycles. A digital twin [...] Read more.
This paper introduces sustainable engineering systems built using digital twin technology and circular economy principles. This research presents a framework for monitoring, modeling, and making decisions in real timusing virtual replicas of physical products, processes, and systems in product lifecycles. A digital twin was used to show that through a digital twin, waste was reduced by 27%, energy consumption was reduced by 32%, and the resource recovery rate increased to 45%. The proposed approach under the framework employs various machine learning algorithms, IoT sensor networks, and advanced data analytics to support closed-loop flows of materials. The results show how digital twins can enhance progress toward the goals the circular economy sets to identify inefficiencies, predict maintenance needs, and optimize the use of resources. This integration is a promising industry approach that will introduce more sustainable operations and maintain economic viability. Full article
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35 pages, 1123 KiB  
Article
AI-Based Bankruptcy Prediction for Agricultural Firms in Central and Eastern Europe
by Dominika Gajdosikova, Jakub Michulek and Irina Tulyakova
Int. J. Financial Stud. 2025, 13(3), 133; https://doi.org/10.3390/ijfs13030133 - 16 Jul 2025
Abstract
The agriculture sector is increasingly challenged to maintain productivity and sustainability amidst environmental, marketplace, and geopolitical pressures. While precision agriculture enhances physical production, the financial resilience of agricultural firms has been understudied. In this study, machine learning (ML) methods, including logistic regression (LR), [...] Read more.
The agriculture sector is increasingly challenged to maintain productivity and sustainability amidst environmental, marketplace, and geopolitical pressures. While precision agriculture enhances physical production, the financial resilience of agricultural firms has been understudied. In this study, machine learning (ML) methods, including logistic regression (LR), decision trees (DTs), and artificial neural networks (ANNs), are employed to predict the bankruptcy risk for Central and Eastern European (CEE) farming firms. All models consistently showed high performance, with AUC values exceeding 0.95. DTs had the highest overall accuracy (95.72%) and F1 score (0.9768), LR had the highest recall (0.9923), and ANNs had the highest discrimination power (AUC = 0.960). Visegrad, Balkan, Baltic, and Eastern Europe subregional models featured economic and structural heterogeneity, reflecting the need for local financial risk surveillance. The results support the development of AI-based early warning systems for agricultural finance, enabling smarter decision-making, regional adaptation, and enhanced sustainability in the sector. Full article
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50 pages, 763 KiB  
Review
A Comprehensive Review on Sensor-Based Electronic Nose for Food Quality and Safety
by Teodora Sanislav, George D. Mois, Sherali Zeadally, Silviu Folea, Tudor C. Radoni and Ebtesam A. Al-Suhaimi
Sensors 2025, 25(14), 4437; https://doi.org/10.3390/s25144437 (registering DOI) - 16 Jul 2025
Abstract
Food quality and safety are essential for ensuring public health, preventing foodborne illness, reducing food waste, maintaining consumer confidence, and supporting regulatory compliance and international trade. This has led to the emergence of many research works that focus on automating and streamlining the [...] Read more.
Food quality and safety are essential for ensuring public health, preventing foodborne illness, reducing food waste, maintaining consumer confidence, and supporting regulatory compliance and international trade. This has led to the emergence of many research works that focus on automating and streamlining the assessment of food quality. Electronic noses have become of paramount importance in this context. We analyze the current state of research in the development of electronic noses for food quality and safety. We examined research papers published in three different scientific databases in the last decade, leading to a comprehensive review of the field. Our review found that most of the efforts use portable, low-cost electronic noses, coupled with pattern recognition algorithms, for evaluating the quality levels in certain well-defined food classes, reaching accuracies exceeding 90% in most cases. Despite these encouraging results, key challenges remain, particularly in diversifying the sensor response across complex substances, improving odor differentiation, compensating for sensor drift, and ensuring real-world reliability. These limitations indicate that a complete device mimicking the flexibility and selectivity of the human olfactory system is not yet available. To address these gaps, our review recommends solutions such as the adoption of adaptive machine learning models to reduce calibration needs and enhance drift resilience and the implementation of standardized protocols for data acquisition and model validation. We introduce benchmark comparisons and a future roadmap for electronic noses that demonstrate their potential to evolve from controlled studies to scalable industrial applications. In doing so, this review aims not only to assess the state of the field but also to support its transition toward more robust, interpretable, and field-ready electronic nose technologies. Full article
(This article belongs to the Special Issue Sensors in 2025)
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18 pages, 282 KiB  
Article
A Qualitative Descriptive Study of Teachers’ Beliefs and Their Design Thinking Practices in Integrating an AI-Based Automated Feedback Tool
by Meerita Kunna Segaran and Synnøve Heggedal Moltudal
Educ. Sci. 2025, 15(7), 910; https://doi.org/10.3390/educsci15070910 (registering DOI) - 16 Jul 2025
Abstract
In this post-digital age, writing assessment has been markedly influenced by advancements in artificial intelligence (AI), emphasizing the role of automated formative feedback in supporting second language (L2) writing. This study investigates how Norwegian teachers use an AI-driven automated feedback tool, the Essay [...] Read more.
In this post-digital age, writing assessment has been markedly influenced by advancements in artificial intelligence (AI), emphasizing the role of automated formative feedback in supporting second language (L2) writing. This study investigates how Norwegian teachers use an AI-driven automated feedback tool, the Essay Assessment Technology (EAT), in process writing for the first time. Framed by the second and third-order barriers framework, we looked at teachers’ beliefs and the design level changes that they made in their teaching. Data were collected in Autumn 2022, during the testing of EAT’s first prototype. Teachers were first introduced to EAT in a workshop. A total of 3 English as a second language teachers from different schools were informants in this study. Teachers then used EAT in the classroom with their 9th-grade students (13 years old). Through individual teacher interviews, this descriptive qualitative study explores teachers’ perceptions, user experiences, and pedagogical decisions when incorporating EAT into their practices. The findings indicate that teachers’ beliefs about technology and its role in student learning, as well as their views on students’ responsibilities in task completion, significantly influence their instructional choices. Additionally, teachers not only adopt AI-driven tools but are also able to reflect and solve complex teaching and learning activities in the classroom, which demonstrates that these teachers have applied design thinking processes in integrating technology in their teaching. Based on the results in this study, we suggest the need for targeted professional development to support effective technology integration. Full article
22 pages, 3659 KiB  
Article
A Proposal of Integration of Universal Design for Learning and Didactic Suitability Criteria
by Alicia Sánchez, Carlos Ledezma and Vicenç Font
Educ. Sci. 2025, 15(7), 909; https://doi.org/10.3390/educsci15070909 (registering DOI) - 16 Jul 2025
Abstract
Given the growing relevance of issues of educational inclusion at an international level, educational curricula have pointed out the need to address the diversity of students in the classroom. In this article, a theoretical reflection is proposed around the Universal Design for Learning [...] Read more.
Given the growing relevance of issues of educational inclusion at an international level, educational curricula have pointed out the need to address the diversity of students in the classroom. In this article, a theoretical reflection is proposed around the Universal Design for Learning (UDL) guideline—as inclusive principles for generic teaching and learning processes—and Didactic Suitability Criteria (DSC) guideline—as specific principles for mathematical teaching and learning processes—to establish relationships and seek complementarities between both references. To this end, firstly, a document analysis of literature about UDL was conducted; secondly, UDL and DSC guidelines were contrasted, relating UDL principles and verification points to DSC components and indicators to design a first proposal of an integrated guideline between both references; and, thirdly, an expert validation was conducted with researchers familiar with DSC to adjust the guideline originally proposed. As a main result, a proposal of integration of the UDL and DSC guidelines was designed, which intends to organise the reflection of (prospective and practising) mathematics teachers on their teaching practice. This integrated proposal not only seeks to address current curricular needs, but also to delve deeper into theoretical development that contributes to refining existing tools to encourage reflection on teaching practice. Full article
(This article belongs to the Special Issue Innovation, Didactics, and Education for Sustainability)
16 pages, 2946 KiB  
Article
AI-Driven Comprehensive SERS-LFIA System: Improving Virus Automated Diagnostics Through SERS Image Recognition and Deep Learning
by Shuai Zhao, Meimei Xu, Chenglong Lin, Weida Zhang, Dan Li, Yusi Peng, Masaki Tanemura and Yong Yang
Biosensors 2025, 15(7), 458; https://doi.org/10.3390/bios15070458 (registering DOI) - 16 Jul 2025
Abstract
Highly infectious and pathogenic viruses seriously threaten global public health, underscoring the need for rapid and accurate diagnostic methods to effectively manage and control outbreaks. In this study, we developed a comprehensive Surface-Enhanced Raman Scattering–Lateral Flow Immunoassay (SERS-LFIA) detection system that integrates SERS [...] Read more.
Highly infectious and pathogenic viruses seriously threaten global public health, underscoring the need for rapid and accurate diagnostic methods to effectively manage and control outbreaks. In this study, we developed a comprehensive Surface-Enhanced Raman Scattering–Lateral Flow Immunoassay (SERS-LFIA) detection system that integrates SERS scanning imaging with artificial intelligence (AI)-based result discrimination. This system was based on an ultra-sensitive SERS-LFIA strip with SiO2-Au NSs as the immunoprobe (with a theoretical limit of detection (LOD) of 1.8 pg/mL). On this basis, a negative–positive discrimination method combining SERS scanning imaging with a deep learning model (ResNet-18) was developed to analyze probe distribution patterns near the T line. The proposed machine learning method significantly reduced the interference of abnormal signals and achieved reliable detection at concentrations as low as 2.5 pg/mL, which was close to the theoretical Raman LOD. The accuracy of the proposed ResNet-18 image recognition model was 100% for the training set and 94.52% for the testing set, respectively. In summary, the proposed SERS-LFIA detection system that integrates detection, scanning, imaging, and AI automated result determination can achieve the simplification of detection process, elimination of the need for specialized personnel, reduction in test time, and improvement of diagnostic reliability, which exhibits great clinical potential and offers a robust technical foundation for detecting other highly pathogenic viruses, providing a versatile and highly sensitive detection method adaptable for future pandemic prevention. Full article
(This article belongs to the Special Issue Surface-Enhanced Raman Scattering in Biosensing Applications)
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22 pages, 2278 KiB  
Article
Super-Resolution for Renewable Energy Resource Data with Wind from Reanalysis Data and Application to Ukraine
by Brandon N. Benton, Grant Buster, Pavlo Pinchuk, Andrew Glaws, Ryan N. King, Galen Maclaurin and Ilya Chernyakhovskiy
Energies 2025, 18(14), 3769; https://doi.org/10.3390/en18143769 - 16 Jul 2025
Abstract
With a potentially increasing share of the electricity grid relying on wind to provide generating capacity and energy, there is an expanding global need for historically accurate, spatiotemporally continuous, high-resolution wind data. Conventional downscaling methods for generating these data based on numerical weather [...] Read more.
With a potentially increasing share of the electricity grid relying on wind to provide generating capacity and energy, there is an expanding global need for historically accurate, spatiotemporally continuous, high-resolution wind data. Conventional downscaling methods for generating these data based on numerical weather prediction have a high computational burden and require extensive tuning for historical accuracy. In this work, we present a novel deep learning-based spatiotemporal downscaling method using generative adversarial networks (GANs) for generating historically accurate high-resolution wind resource data from the European Centre for Medium-Range Weather Forecasting Reanalysis version 5 data (ERA5). In contrast to previous approaches, which used coarsened high-resolution data as low-resolution training data, we use true low-resolution simulation outputs. We show that by training a GAN model with ERA5 as the low-resolution input and Wind Integration National Dataset Toolkit (WTK) data as the high-resolution target, we achieved results comparable in historical accuracy and spatiotemporal variability to conventional dynamical downscaling. This GAN-based downscaling method additionally reduces computational costs over dynamical downscaling by two orders of magnitude. We applied this approach to downscale 30 km, hourly ERA5 data to 2 km, 5 min wind data for January 2000 through December 2023 at multiple hub heights over Ukraine, Moldova, and part of Romania. With WTK coverage limited to North America from 2007–2013, this is a significant spatiotemporal generalization. The geographic extent centered on Ukraine was motivated by stakeholders and energy-planning needs to rebuild the Ukrainian power grid in a decentralized manner. This 24-year data record is the first member of the super-resolution for renewable energy resource data with wind from the reanalysis data dataset (Sup3rWind). Full article
28 pages, 5813 KiB  
Article
YOLO-SW: A Real-Time Weed Detection Model for Soybean Fields Using Swin Transformer and RT-DETR
by Yizhou Shuai, Jingsha Shi, Yi Li, Shaohao Zhou, Lihua Zhang and Jiong Mu
Agronomy 2025, 15(7), 1712; https://doi.org/10.3390/agronomy15071712 - 16 Jul 2025
Abstract
Accurate weed detection in soybean fields is essential for enhancing crop yield and reducing herbicide usage. This study proposes a YOLO-SW model, an improved version of YOLOv8, to address the challenges of detecting weeds that are highly similar to the background in natural [...] Read more.
Accurate weed detection in soybean fields is essential for enhancing crop yield and reducing herbicide usage. This study proposes a YOLO-SW model, an improved version of YOLOv8, to address the challenges of detecting weeds that are highly similar to the background in natural environments. The research stands out for its novel integration of three key advancements: the Swin Transformer backbone, which leverages local window self-attention to achieve linear O(N) computational complexity for efficient global context capture; the CARAFE dynamic upsampling operator, which enhances small target localization through context-aware kernel generation; and the RTDETR encoder, which enables end-to-end detection via IoU-aware query selection, eliminating the need for complex post-processing. Additionally, a dataset of six common soybean weeds was expanded to 12,500 images through simulated fog, rain, and snow augmentation, effectively resolving data imbalance and boosting model robustness. The experimental results highlight both the technical superiority and practical relevance: YOLO-SW achieves 92.3% mAP@50 (3.8% higher than YOLOv8), with recognition accuracy and recall improvements of 4.2% and 3.9% respectively. Critically, on the NVIDIA Jetson AGX Orin platform, it delivers a real-time inference speed of 59 FPS, making it suitable for seamless deployment on intelligent weeding robots. This low-power, high-precision solution not only bridges the gap between deep learning and precision agriculture but also enables targeted herbicide application, directly contributing to sustainable farming practices and environmental protection. Full article
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21 pages, 31160 KiB  
Article
Local Information-Driven Hierarchical Fusion of SAR and Visible Images via Refined Modal Salient Features
by Yunzhong Yan, La Jiang, Jun Li, Shuowei Liu and Zhen Liu
Remote Sens. 2025, 17(14), 2466; https://doi.org/10.3390/rs17142466 - 16 Jul 2025
Abstract
Compared to other multi-source image fusion tasks, visible and SAR image fusion faces a lack of training data in deep learning-based methods. Introducing structural priors to design fusion networks is a viable solution. We incorporated the feature hierarchy concept from computer vision, dividing [...] Read more.
Compared to other multi-source image fusion tasks, visible and SAR image fusion faces a lack of training data in deep learning-based methods. Introducing structural priors to design fusion networks is a viable solution. We incorporated the feature hierarchy concept from computer vision, dividing deep features into low-, mid-, and high-level tiers. Based on the complementary modal characteristics of SAR and visible, we designed a fusion architecture that fully analyze and utilize the difference of hierarchical features. Specifically, our framework has two stages. In the cross-modal enhancement stage, a CycleGAN generator-based method for cross-modal interaction and input data enhancement is employed to generate pseudo-modal images. In the fusion stage, we have three innovations: (1) We designed feature extraction branches and fusion strategies differently for each level based on the features of different levels and the complementary modal features of SAR and visible to fully utilize cross-modal complementary features. (2) We proposed the Layered Strictly Nested Framework (LSNF), which emphasizes hierarchical differences and uses hierarchical characteristics, to reduce feature redundancy. (3) Based on visual saliency theory, we proposed a Gradient-weighted Pixel Loss (GWPL), which dynamically assigns higher weights to regions with significant gradient magnitudes, emphasizing high-frequency detail preservation during fusion. Experiments on the YYX-OPT-SAR and WHU-OPT-SAR datasets show that our method outperforms 11 state-of-the-art methods. Ablation studies confirm each component’s contribution. This framework effectively meets remote sensing applications’ high-precision image fusion needs. Full article
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32 pages, 6589 KiB  
Article
Machine Learning (AutoML)-Driven Wheat Yield Prediction for European Varieties: Enhanced Accuracy Using Multispectral UAV Data
by Krstan Kešelj, Zoran Stamenković, Marko Kostić, Vladimir Aćin, Dragana Tekić, Tihomir Novaković, Mladen Ivanišević, Aleksandar Ivezić and Nenad Magazin
Agriculture 2025, 15(14), 1534; https://doi.org/10.3390/agriculture15141534 - 16 Jul 2025
Abstract
Accurate and timely wheat yield prediction is valuable globally for enhancing agricultural planning, optimizing resource use, and supporting trade strategies. Study addresses the need for precision in yield estimation by applying machine-learning (ML) regression models to high-resolution Unmanned Aerial Vehicle (UAV) multispectral (MS) [...] Read more.
Accurate and timely wheat yield prediction is valuable globally for enhancing agricultural planning, optimizing resource use, and supporting trade strategies. Study addresses the need for precision in yield estimation by applying machine-learning (ML) regression models to high-resolution Unmanned Aerial Vehicle (UAV) multispectral (MS) and Red-Green-Blue (RGB) imagery. Research analyzes five European wheat cultivars across 400 experimental plots created by combining 20 nitrogen, phosphorus, and potassium (NPK) fertilizer treatments. Yield variations from 1.41 to 6.42 t/ha strengthen model robustness with diverse data. The ML approach is automated using PyCaret, which optimized and evaluated 25 regression models based on 65 vegetation indices and yield data, resulting in 66 feature variables across 400 observations. The dataset, split into training (70%) and testing sets (30%), was used to predict yields at three growth stages: 9 May, 20 May, and 6 June 2022. Key models achieved high accuracy, with the Support Vector Regression (SVR) model reaching R2 = 0.95 on 9 May and R2 = 0.91 on 6 June, and the Multi-Layer Perceptron (MLP) Regressor attaining R2 = 0.94 on 20 May. The findings underscore the effectiveness of precisely measured MS indices and a rigorous experimental approach in achieving high-accuracy yield predictions. This study demonstrates how a precise experimental setup, large-scale field data, and AutoML can harness UAV and machine learning’s potential to enhance wheat yield predictions. The main limitations of this study lie in its focus on experimental fields under specific conditions; future research could explore adaptability to diverse environments and wheat varieties for broader applicability. Full article
(This article belongs to the Special Issue Applications of Remote Sensing in Agricultural Soil and Crop Mapping)
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25 pages, 765 KiB  
Review
The Latest Advances in Omics Technology for Assessing Tissue Damage: Implications for the Study of Sudden Cardiac Death
by Raluca-Maria Căținaș and Sorin Hostiuc
Int. J. Mol. Sci. 2025, 26(14), 6818; https://doi.org/10.3390/ijms26146818 - 16 Jul 2025
Abstract
Sudden cardiac death (SCD) is a major public health concern, being a leading cause of death worldwide. SCD is particularly alarming for individuals with apparently good health, as it often occurs without preceding warning signs. Unfortunately, traditional autopsy methods frequently fail to identify [...] Read more.
Sudden cardiac death (SCD) is a major public health concern, being a leading cause of death worldwide. SCD is particularly alarming for individuals with apparently good health, as it often occurs without preceding warning signs. Unfortunately, traditional autopsy methods frequently fail to identify the precise cause of death in these cases, highlighting the need for advanced techniques to elucidate underlying mechanisms. Recent advances in molecular biology over the past few years, particularly in proteomics, transcriptomics, and metabolomics techniques, have led to an expanded understanding of gene expression, protein activity, and metabolic changes, offering valuable insights into fatal cardiac events. Combining multi-omics methods with bioinformatics and machine learning algorithms significantly enhances our ability to uncover the processes behind lethal cardiac dysfunctions by identifying new useful biomarkers (like cardiac myosin-binding protein C, acylcarnitines, or microRNAs) to reveal molecular pathways linked to SCD. This narrative review summarizes the role of multi-omics approaches in forensic diagnosis by exploring current applications in unexplained cases and the benefits of integrating merged techniques in otherwise negative autopsies. We also discuss the potential for developing personalized and preventive forensic medicine, the technical limitations of currently available methods, and the ethical considerations arising from these advancements. Full article
(This article belongs to the Special Issue Molecular Biological Determination of Physical Injury)
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13 pages, 223 KiB  
Article
Training of Future Teachers in the Binomial Universal Design for Learning and Technologies for Inclusive Education
by Rosalía Romero-Tena, Raquel Martínez-Navarro and Antonio León-Garrido
Sustainability 2025, 17(14), 6504; https://doi.org/10.3390/su17146504 - 16 Jul 2025
Abstract
Teacher education plays a key role in promoting inclusion and educational equity, especially in contexts characterised by increasing socio-cultural diversity and technological advancement. In this framework, Universal Design for Learning (UDL) and digital technologies are presented as complementary and innovative strategies to create [...] Read more.
Teacher education plays a key role in promoting inclusion and educational equity, especially in contexts characterised by increasing socio-cultural diversity and technological advancement. In this framework, Universal Design for Learning (UDL) and digital technologies are presented as complementary and innovative strategies to create accessible, flexible, and motivating learning environments for all students. The study analysed the impact of UDL-focused learning activities and integrated Information and Communication Technologies (ICT). A comparative tool was applied before and after the intervention to measure the level of knowledge, perception, and digital competence of prospective teachers. Statistical analyses were carried out to evaluate the changes obtained. Findings reveal significant improvements in knowledge about UDL, as well as positive perceptions of ICT as a resource for inclusion. Participants demonstrated a greater understanding of UDL principles and strengthened their digital competences to design educational proposals adapted to diversity. The research confirms the value of integrating UDL and ICT in teacher training, fostering inclusive educational practices. It highlights the need to strengthen training programmes that respond to the current challenges of the education system. Full article
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