The continuous increase in sequenced genomes in public repositories makes the choice of interesting bacterial strains for future sequencing projects ever more complicated, as it is difficult to estimate the redundancy between these strains and the al...
The tropospheric and stratospheric temperature trends and uncertainties in the fifth Coupled Model Intercomparison Project (CMIP5) model simulations in the period of 1979–2005 have been compared with satellite observations. The satellite data include...
Full-length SSU rRNA gene sequencing allows species-level identification of the microorganisms present in milk samples. Here, we used bulk-tank raw milk samples of two German dairies and detected, using this method, a great diversity of bacteria, arc...
The RNA polymerase II (Pol II) transcription process is coordinated by the reversible phosphorylation of its largest subunit-carboxy terminal domain (CTD). Ssu72 is identified as a CTD phosphatase with specificity for phosphorylation of Ser5 and Ser7...
In Mexico, Plasmodium vivax transmission has been confined to the northwestern and southern regions since 2000. Parasites from five malaria foci were analyzed using three genetic markers. The circumsporozoite gene was examined by PCR-RFLP and sequenc...
More than 70% of eukaryotic proteins are regulated by phosphorylation. However, the mechanism of dephosphorylation that counteracts phosphorylation is less studied. Phosphatases are classified into 104 distinct groups based on substrate-specific feat...
While nuclear small subunit ribosomal DNA (nSSU rDNA) is the most commonly-used gene marker in studying phylogeny, ecology, abundance, and biodiversity of microbial eukaryotes, mitochondrial small subunit ribosomal DNA (mtSSU rDNA) provides an altern...
Metabarcoding and high-throughput sequencing methods have greatly improved our understanding of protist diversity. Although the V4 region of small subunit ribosomal DNA (SSU-V4 rDNA) is the most widely used marker in DNA metabarcoding of eukaryotic m...
As qualified microscopy of enteric parasitoses as defined by high diagnostic accuracy is difficult to maintain in non-endemic areas due to scarce opportunities for practicing with positive sample materials, molecular diagnostic options provide less i...
As one of the most imperative antioxidants in higher plants, carotenoids serve as accessory pigments to harvest light for photosynthesis and photoprotectors for plants to adapt to high light stress. Here, we report a small subunit (SSU) of geranylger...
The symbiosis and beneficial effects of arbuscular mycorrhizal fungi (AM fungi) on plants have been widely reported; however, the effects might be unascertained in tomato industry production with coconut coir due to the nutrition solution supply, or...
In this study, we address the criteria for the identification of species and subspecific categories (ribosomal lineages) within the genus Entamoeba using an evolutionary species concept and an integrative taxonomic framework, in which genetic evidenc...
Several control mechanisms of eukaryotic gene expression target the initiation step of mRNA translation. The canonical translation initiation pathway begins with cap-dependent attachment of the small ribosomal subunit (SSU) to the messenger ribonucle...
Three Dysteria species, D. crassipes Claparède & Lachmann, 1859; D. brasiliensis Faria et al., 1922; and D. paracrassipes n. sp., were collected from subtropical coastal waters of the East China Sea, near Ningbo, China. The three species w...
The majority of myxosporean species (Cnidaria: Myxozoa) of the genera Myxobolus (35 species), Henneguya (8 species), and Myxidium (9 species) from freshwater or brackish fish in Japan were recorded more than 30 years ago (accumulatively 81.1% [43/53]...
Two new benthic freshwater species belonging to the genus Achnanthidium were found in Korea. Achnanthidium ovale sp. nov. and A. cavitatum sp. nov. are described as new species based on light and scanning electron microscopy observations and molecula...
A strain of oogamous biflagellate green alga was isolated during a study on soil algal diversity in the Russian Far East (Sakhalin Region, Iturup Island) and examined using an integrative approach. Phylogenetic analyses, based on the SSU rDNA gene, r...
Ciliates of the genus Frontonia have been extensively studied to resolve their phylogenetic and evolutionary history, but challenges remain. This study used molecular analyses of SSU rRNA genes, phylogenetic tree reconstruction, molecular dating, and...
Secondary Users (SUs) are allowed to use the temporarily unused licensed spectrum without disturbing Primary Users (PUs) in Cognitive Radio Ad Hoc Networks (CRAHNs). Existing architectures for CRAHNs impose energy-consuming Cognitive Radios (CRs) on...
Sulfiting agents are common preservatives in the food and beverage industry to inhibit spoilage microorganisms. Sulfite produced by the dissolution of sulfur dioxide (SO2) in water is used as a microbial inhibitor and antioxidant during winemaking. T...
In order to investigate the infection status of Cryptosporidium in O. curzoniae and B. grunniens in Zoige County, Sichuan Province, fecal samples from B. grunniens and gastrointestinal contents from captured O. curzoniae were collected between March...
The small nucleolar RNA snR30 (U17 in humans) plays a unique role during ribosome synthesis. Unlike most members of the H/ACA class of guide RNAs, the small nucleolar ribonucleoprotein (snoRNP) complex assembled on snR30 does not direct pseudouridyla...
A variety of rodent ceca are parasitized by Tritrichomonas muris (T. muris), a flagellated protozoan. To date, there are no ideal methods for the detection of T. muris infections in laboratory mice; thus, new molecular methodologies for its specific...
Nuclear ribosomal and mitochondrial genes have been utilized individually or in combination to identify known species and discriminate closely related species. However, compared with metazoans, genetic diversity within the ciliate order Euplotida is...
Over the years, cases of autochthonous leishmaniasis have been dramatically increasing in Thailand. Recently, several publications have claimed certain species of the phlebotomine sand flies and biting midges potentially serve as natural vectors of L...
Soil ciliates are an important component of the soil micro-food web, playing key roles in organic matter decomposition and nutrient cycling. However, research on the species diversity and taxonomy of this group in the temperate forest soils of China...
Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validit...
Many filamentous and sarcinoid terrestrial or freshwater green algae that were previously assigned to the Chlorophyceae are members of lineages belonging to the class Ulvophyceae. One of these lineages is the Planophila-clade (Ulotrichales). Some gen...
Chlorella-like green algae that reproduce only asexually by immotile autospores or motile zoospores are the most typical inhabitants of non-aquatic environments. They have a simple morphology that hampers their differentiation, but algae of such habi...
A new species, Cryptomonas tropica sp. nov., is described from Cat Tien National Park (Vietnam) based on morphological and molecular data. Strains of the new species were isolated from soil, which is an unusual environment for photosynthetic cryptomo...
Heterolobose amoebae are important members of marine, freshwater, and soil microbial communities, but their diversity remains under-explored. We studied the diversity of Vahlkampfiidae to improve our understanding of heterolobosean relationships and...
In this study we identified single nucleotide polymorphism (SNP) and sequence characteristic amplification region (SCAR) markers for specific identification of antler-shaped Ganoderma lucidum strains. When the partial mitochondrial SSU rDNA gene sequ...
The monotypic genus Prasiolopsis has been known for a long time, but is often overlooked because of difficulties in identification and the morphological variability between uniseriate filaments and cell packages forming pseudoparenchymatic thalli dep...
Although hypersaline environments pose challenges to life because of the low water content (water activity), many such habitats appear to support eukaryotic microbes. This contribution presents brief reviews of our current knowledge on eukaryotes of...
A newly discovered myxosporean parasite was described from the gallbladder of Nemipterus virgatus Houttuyn, 1782 collected from the East China Sea. Mature myxospores are crescent-shaped with shell valves that taper gradually toward rounded ends. Each...
We report an Illumina high-throughput sequencing protocol of eukaryotic microbes in the world’s deepest marine blue hole, Sansha Yongle Blue Hole, Xisha, South China Sea. The variable V9 region of small subunit (SSU) rDNA, was sequenced using t...
Since most studies about community structures of microorganisms in Antarctic terrestrial lakes using molecular biological tools are mainly focused on bacteria, limited information is available about archaeal and eukaryotic microbial diversity. In thi...
Free-living amoebae (FLAs) are widely present in the environment and may be pathogenic for animals and humans. Studies on the prevalence of FLAs in European soils are few in number. This study aimed to molecularly identify the species and genotypes o...
Most marine coccoid and sarcinoid green algal species have traditionally been placed within genera dominated by species from freshwater or soil habitats. For example, the genera Chlorocystis and Halochlorococcum contain exclusively marine species; ho...
29 November 2020
Diabetes mellitus (DM) is a major global public health problem with an increasing prevalence. DM increases the risk of infections caused by bacteria, fungi, viruses, and parasites. We examined the prevalence, subtypes, and risk factors of Blastocysti...
Environmental filtering studies have revealed immense oceanic microbial diversity, yet the Southeast Pacific remains comparatively undersampled. We characterize the molecular diversity of phytoplankton across two biogeographic domains with contrastin...
The genus Pleuronema Dujardin, 1841, with nearly 40 morphospecies, is one of the largest genera in the well-known subclass Scuticociliatia. In the present study, two Pleuronema species were collected from subtropical coastal waters of the East China...
Marine protists are essential for globally critical biological processes, including the biogeochemical cycles of matter and energy. However, compared with their prokaryotic counterpart, it remains largely unclear how environmental factors determine t...
Two new species of free-living marine nematodes, Metachromadora parobscura sp. nov. and Molgolaimus longicaudatus sp. nov., from mangrove wetlands of Beihai, Guangxi province in China, are described. Metachromadora parobscura sp. nov. is characterize...
Traditionally, the order Ulotrichales comprised green algae of an unbranched, uniseriate, filamentous morphology. However, since the establishment of ultrastructural features, the circumscription of this order has dramatically changed. Some genera an...
Harmful algal blooms (HABs) occur worldwide, causing health problems and economic damages to fisheries and tourism. Monitoring agencies are therefore essential, yet monitoring is based only on time-consuming light microscopy, a level at which a corre...
Fine nematodes of the family Capillariidae parasitize various organs and tissues in fish, amphibians, reptiles, avians, and mammals. Currently classified into more than 20 genera, these nematodes are primarily distinguished based on the caudal struct...
Distribution of blood fluke Aporocotyle spp. parasitizing Merluccius species from the coasts of South America (Peru, Chile and Argentina) constitutes an excellent opportunity to evaluate the geographical amplitude in which a parasite can exploit the...
Microsporidia are natural pathogens of arthropods and have been used as biological control against insect pests. In the United States, efforts to control the invasive Red Imported Fire Ant, Solenopsis invicta, and Black Imported Fire Ant, Solenopsis ...
The green sunfish, Lepomis cyanellus, is a common centrarchid that has been previously reported to harbor several myxosporeans. In May 2022, six L. cyanellus were collected from the Caddo River, Montgomery County, Arkansas, USA, and had their gills,...
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