Next Article in Journal
Acknowledgement to Reviewers of Microorganisms in 2018
Previous Article in Journal
Exploitation of Three Non-Conventional Yeast Species in the Brewing Process
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Microorganisms 2019, 7(1), 12; https://doi.org/10.3390/microorganisms7010012

SNP and SCAR Markers for Specific Discrimination of Antler-Shaped Ganoderma lucidum

1
Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun 565-851, Korea
2
Department of Integrated Biosciences, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University, Chungju-si 27478, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 1 December 2018 / Revised: 31 December 2018 / Accepted: 8 January 2019 / Published: 9 January 2019
(This article belongs to the Section Molecular Microbiology)
Full-Text   |   PDF [3492 KB, uploaded 9 January 2019]   |  

Abstract

In this study we identified single nucleotide polymorphism (SNP) and sequence characteristic amplification region (SCAR) markers for specific identification of antler-shaped Ganoderma lucidum strains. When the partial mitochondrial SSU rDNA gene sequence of various antler- and kidney-shaped G. lucidum strains were analyzed and aligned, an SNP was found only in the antler-shaped G. lucidum strain at position 456 bp. In addition, this SNP of antler-shaped strains was digested by HinfI restriction enzyme. We further analyzed the polymorphism of various G. lucidum strains by random amplified polymorphic DNA (RAPD) analysis. In RAPD analysis, we isolated and sequenced a fragment, specific for antler-shaped G. lucidum strains. Based on this specific fragment sequence, two sets of specific primer pairs for antler-shaped G. lucidum strains were designed. PCR analysis revealed that two specific bands were observed only from antler-shaped strains. These two molecular markers will be helpful for identification of morphological characteristics of G. lucidum. View Full-Text
Keywords: antler-shape; Ganoderma lucidum; kidney-shape; mitochondrial SSU rDNA; SCAR marker; SNP marker antler-shape; Ganoderma lucidum; kidney-shape; mitochondrial SSU rDNA; SCAR marker; SNP marker
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Kwon, O.-C.; Lee, C.-S.; Park, Y.-J. SNP and SCAR Markers for Specific Discrimination of Antler-Shaped Ganoderma lucidum. Microorganisms 2019, 7, 12.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top