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32 Results Found

  • Article
  • Open Access
1 Citations
2,093 Views
16 Pages

17 February 2024

We have described a new route for the preparation of partially methylated polygalacturonic acid containing hydrolyzed (acidic) and unhydrolyzed (methyl esterified) carboxylate groups in a ratio of 1:1 (PGA, compound 1), and one of its basic FeIII&mda...

  • Feature Paper
  • Article
  • Open Access
965 Views
18 Pages

14 May 2025

Polygalacturonic acid (PGA), derived from the natural plant polysaccharide, pectin, has been suggested as a biomaterial for implantable medical devices and tissue engineering; particularly in the field of bone implant materials. As a negatively charg...

  • Article
  • Open Access
2 Citations
5,036 Views
9 Pages

24 February 2025

Candida colonization and biofilms are significant contributors to impaired wound healing. Consequently, improved treatments are needed to eradicate Candida biofilms in wounds. Wounds present complex biofilm extracellular matrix environments, with mic...

  • Article
  • Open Access
17 Citations
3,897 Views
13 Pages

The Effect of a New Coating on the Drying Performance of Fruit and Vegetables Products: Experimental Investigation and Artificial Neural Network Modeling

  • S. M. Atiqure Rahman,
  • Ahmed M. Nassef,
  • Mujahed Al-Dhaifallah,
  • Mohammad Ali Abdelkareem and
  • Hegazy Rezk

9 March 2020

A study on mass transfer using new coating materials (namely alginic acid and polygalacturonic acid) during osmotic dehydration—and hence in a laboratory-scale convective dryer to evaluate drying performance—was carried out. Potato and ap...

  • Article
  • Open Access
1,430 Views
14 Pages

Toluidine Blue for the Determination of Binding of Anionic Polysaccharides to Lipid Raft Domains by Absorption Spectroscopy

  • Sandra Gębczyńska,
  • Julia Gdowska,
  • Agata Mikos,
  • Iga Gawrońska,
  • Teresa Janas,
  • Aleksander Czogalla and
  • Tadeusz Janas

The complexes of negatively charged polysaccharides with lipid vesicles have been shown to have applications in medicine, bioremediation, water purification, and construction of nano-biosensors. This article presents research on the formation of thes...

  • Article
  • Open Access
6 Citations
2,419 Views
16 Pages

Characterization of Two Novel Rumen-Derived Exo-Polygalacturonases: Catalysis and Molecular Simulations

  • Qian Deng,
  • Xiaobao Sun,
  • Deying Gao,
  • Yuting Wang,
  • Yu Liu,
  • Nuo Li,
  • Zhengguang Wang,
  • Mingqi Liu,
  • Jiakun Wang and
  • Qian Wang

Pectinases are a series of enzymes that degrade pectin and have been used extensively in the food, feed, and textile industries. The ruminant animal microbiome is an excellent source for mining novel pectinases. Two polygalacturonase genes, IDSPga28-...

  • Article
  • Open Access
7 Citations
2,586 Views
10 Pages

Cloning and Functional Identification of SlPG49 in Solanum lycopersicum

  • Weiqiang Li,
  • Liai Xu,
  • Rui Xia,
  • Ying Shen,
  • Zhujun Zhu,
  • Youjian Yu and
  • Yunxiang Zang

3 December 2021

The modification and degradation of pectin in cell walls are necessary for the fruit softening process, which involves a series of pectin-modifying enzymes. Polygalacturonases (PGs) are a major group of pectin-hydrolyzing enzymes, which participate i...

  • Article
  • Open Access
29 Citations
8,605 Views
13 Pages

Polymeric Nano-Micelles as Novel Cargo-Carriers for LY2157299 Liver Cancer Cells Delivery

  • Nemany Abdelhamid Nemany Hanafy,
  • Alessandra Quarta,
  • Marzia Maria Ferraro,
  • Luciana Dini,
  • Concetta Nobile,
  • Maria Luisa De Giorgi,
  • Sonia Carallo,
  • Cinzia Citti,
  • Antonio Gaballo and
  • Stefano Leporatti
  • + 3 authors

LY2157299 (LY), which is very small molecule bringing high cancer diffusion, is a pathway antagonist against TGFβ. LY dosage can be diluted by blood plasma, can be captured by immune system or it might be dissolved during digestion in gastrointestina...

  • Article
  • Open Access
9 Citations
2,665 Views
14 Pages

28 July 2023

This study aimed to compare the in vitro fermentation characteristics of polysaccharides from Bergamot and Laoxianghuang (fermented 1, 3, and 5 years from bergamot) using the stable in vitro human gut fermentation model. Results showed that bergamot...

  • Article
  • Open Access
25 Citations
7,355 Views
13 Pages

Cloning, Expression and Characterization of a Novel Thermophilic Polygalacturonase from Caldicellulosiruptor bescii DSM 6725

  • Yanyan Chen,
  • Dejun Sun,
  • Yulai Zhou,
  • Liping Liu,
  • Weiwei Han,
  • Baisong Zheng,
  • Zhi Wang and
  • Zuoming Zhang

3 April 2014

We cloned the gene ACM61449 from anaerobic, thermophilic Caldicellulosiruptor bescii, and expressed it in Escherichia coli origami (DE3). After purification through thermal treatment and Ni-NTA agarose column extraction, we characterized the proper...

  • Review
  • Open Access
16 Citations
8,588 Views
18 Pages

Source, Extraction, Properties, and Multifunctional Applications of Pectin: A Short Review

  • Le Yi,
  • Lifeng Cheng,
  • Qi Yang,
  • Ke Shi,
  • Fengbo Han,
  • Wei Luo and
  • Shengwen Duan

12 October 2024

Pectin, a heteropolysaccharide derived from plant cell walls, is essential in the food, pharmaceutical, and environmental industries. Currently, citrus and apple peels are the primary sources for commercial pectin production. The yield and quality of...

  • Article
  • Open Access
29 Citations
5,859 Views
19 Pages

4 November 2021

Despite more than a century of research on the hydration of biomolecules, the hydration of carbohydrates is insufficiently studied. An approach to studying dynamic hydration shells of carbohydrates in aqueous solutions based on terahertz time-domain...

  • Article
  • Open Access
29 Citations
4,380 Views
14 Pages

26 October 2018

Pectate lyase (EC 4.2.2.2) catalyzes the cleavage of α-1,4-glycosidic bonds of pectin polymers, and it has potential uses in the textile industry. In this study, a novel pectate lyase belonging to polysaccharide lyase family 10 was screened fro...

  • Review
  • Open Access
78 Citations
9,381 Views
21 Pages

Pectin: A Bioactive Food Polysaccharide with Cancer Preventive Potential

  • Talha Bin Emran,
  • Fahadul Islam,
  • Saikat Mitra,
  • Shyamjit Paul,
  • Nikhil Nath,
  • Zidan Khan,
  • Rajib Das,
  • Deepak Chandran,
  • Rohit Sharma and
  • Raquel P. F. Guiné
  • + 4 authors

31 October 2022

Pectin is an acidic heteropolysaccharide found in the cell walls and the primary and middle lamella of land plants. To be authorized as a food additive, industrial pectins must meet strict guidelines set forth by the Food and Agricultural Organizatio...

  • Article
  • Open Access
6 Citations
3,583 Views
16 Pages

Rice RBH1 Encoding A Pectate Lyase is Critical for Apical Panicle Development

  • Dong He,
  • Rui Liang,
  • Tuan Long,
  • Ying Yang and
  • Changyin Wu

30 January 2021

Panicle morphology is one of the main determinants of the rice yield. Panicle abortion, a typical panicle morphological defect results in yield reduction due to defective spikelet development. To further elucidate the molecular mechanism of panicle a...

  • Article
  • Open Access
7 Citations
4,250 Views
16 Pages

Partial Purification and Characterization of Exo-Polygalacturonase Produced by Penicillium oxalicum AUMC 4153

  • Shamsan A. Almowallad,
  • Ghedeir M. Alshammari,
  • Muneer M. Alsayadi,
  • Naofel Aljafer,
  • Ekram A. Al-Sanea,
  • Mohammed Abdo Yahya and
  • Laila Naif Al-Harbi

14 February 2022

Pectinase enzymes are important industrial enzymes having considerable applications in several industries, especially in food processing. Pectinases contribute 25% of global food enzyme sales. Therefore, the demand for a commercial enzyme with desira...

  • Article
  • Open Access
33 Citations
7,978 Views
20 Pages

Production and Properties of a Thermostable, pH—Stable Exo-Polygalacturonase Using Aureobasidium pullulans Isolated from Saharan Soil of Algeria Grown on Tomato Pomace

  • Leila Bennamoun,
  • Serge Hiligsmann,
  • Scheherazad Dakhmouche,
  • Amel Ait-Kaki,
  • Fatima-Zohra Kenza Labbani,
  • Tahar Nouadri,
  • Zahia Meraihi,
  • Benedetta Turchetti,
  • Pietro Buzzini and
  • Philippe Thonart

29 October 2016

Polygalacturonase is a valuable biocatalyst for several industrial applications. Production of polygalacturonase using the Aureobasidium pullulans stain isolated from Saharan soil of Algeria was investigated. Its capacity to produce polygalacturonase...

  • Article
  • Open Access
2 Citations
3,080 Views
15 Pages

26 January 2022

Fluorescently labeled, solute-binding proteins that change their fluorescent output in response to ligand binding are frequently used as biosensors for a wide range of applications. We have previously developed a “Computational Identification o...

  • Article
  • Open Access
10 Citations
3,566 Views
15 Pages

Genome-Wide Association Study Identifies Rice Panicle Blast-Resistant Gene Pb4 Encoding a Wall-Associated Kinase

  • Yunxin Fan,
  • Lu Ma,
  • Xiaoqian Pan,
  • Pujiang Tian,
  • Wei Wang,
  • Kunquan Liu,
  • Ziwei Xiong,
  • Changqing Li,
  • Zhixue Wang and
  • Yongmei Bao
  • + 2 authors

Rice blast is one of the most devastating diseases, causing a significant reduction in global rice production. Developing and utilizing resistant varieties has proven to be the most efficient and cost-effective approach to control blasts. However, du...

  • Article
  • Open Access
1 Citations
1,409 Views
17 Pages

4 September 2025

Alkaline pectate lyases hold significant promise for various industrial applications, including the degumming processes in papermaking and textiles. In this study, a novel pectinase, PelA, derived from a strain of Paenibacillus borealis, was characte...

  • Article
  • Open Access
634 Views
13 Pages

Effects of Polysaccharides Extracted from Stem Barks on the Spontaneous Contractile Activity of the Ileal Smooth Muscle

  • Ericka Lorleil Mayindza Ekaghba,
  • Olivier Perruchon,
  • Patrice Lerouge and
  • Line Edwige Mengome

28 July 2025

Decoctions of stem barks from Aucoumea klaineana, Canarium schweinfurthii, Pentadesma butyracea and Scorodophloeus zenkeri are used against affections of irritable bowel syndrome in Gabonese traditional medicine. In the present study, we aim to deter...

  • Article
  • Open Access
16 Citations
3,407 Views
12 Pages

Overexpression and Biochemical Characterization of an Endo-α-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris

  • Hua Xu,
  • Pengfei Zhang,
  • Yuchen Zhang,
  • Zebin Liu,
  • Xuebing Zhang,
  • Zhimin Li,
  • Jian-Jun Li and
  • Yuguang Du

Pectinases have many applications in the industry of food, paper, and textiles, therefore finding novel polygalacturonases is required. Multiple sequence alignment and phylogenetic analysis of AnEPG (an endo-α-1,4-polygalacturonase from Aspergi...

  • Project Report
  • Open Access
4 Citations
1,336 Views
13 Pages

5 November 2024

The study aimed to investigate the effects of thifluzamide (2.67 mg/L) on ‘Huangguan’ pear fruit rot caused by Rhizoctonia solani during storage, as well as the activities of polygalacturonase (PG), pectin methylesterase (PME), polygalact...

  • Article
  • Open Access
7 Citations
4,223 Views
18 Pages

Therapeutic Effect of Polymeric Nanomicelles Formulation of LY2157299-Galunisertib on CCl4-Induced Liver Fibrosis in Rats

  • Elisa Panzarini,
  • Stefano Leporatti,
  • Bernardetta Anna Tenuzzo,
  • Alessandra Quarta,
  • Nemany A. N. Hanafy,
  • Gianluigi Giannelli,
  • Camilla Moliterni,
  • Diana Vardanyan,
  • Carolina Sbarigia and
  • Luciana Dini
  • + 2 authors

1 November 2022

Hepatic fibrosis (HF) is a major cause of liver-related disorders and together with cancer-associated fibroblasts can favor liver cancer development by modulating the tumor microenvironment. Advanced HF, characterized by an excess of extracellular ma...

  • Article
  • Open Access
12 Citations
7,376 Views
13 Pages

3 April 2015

Fusarium wilt (Panama disease) caused by Fusarium oxysporum f. sp. cubense (FOC) represents a significant threat to banana (Musa spp.) production. Musa AAB is susceptible to Race 1 (FOC1) and Race 4 (FOC4), while Cavendish Musa AAA is found to be res...

  • Article
  • Open Access
14 Citations
4,075 Views
12 Pages

Characterization of Novel Pectinolytic Enzymes Derived from the Efficient Lignocellulose Degradation Microbiota

  • Qin Miao,
  • Xiaoling Zhang,
  • Yitong Wang,
  • Xiaoqi Li,
  • Zheng Wang,
  • Lingmin Tian,
  • Lingbo Qu and
  • Yongjun Wei

29 September 2022

Diverse pectinolytic enzymes are widely applied in the food, papermaking, and other industries, and they account for more than 25% of the global industrial enzyme demands. Efficient lignocellulose degradation microbiota are reservoirs of pectinolytic...

  • Article
  • Open Access
19 Citations
4,185 Views
18 Pages

Membrane Vesicles of Pectobacterium as an Effective Protein Secretion System

  • Joanna Jonca,
  • Malgorzata Waleron,
  • Paulina Czaplewska,
  • Aleksandra Bogucka,
  • Aleksandra Steć,
  • Szymon Dziomba,
  • Jacek Jasiecki,
  • Michał Rychłowski and
  • Krzysztof Waleron

22 November 2021

Bacteria of genus Pectobacterium are Gram-negative rods of the family Pectobacteriaceae. They are the causative agent of soft rot diseases of crops and ornamental plants. However, their virulence mechanisms are not yet fully elucidated. Membrane vesi...

  • Article
  • Open Access
17 Citations
3,616 Views
19 Pages

Cationic Lignocellulose Nanofibers from Agricultural Waste as High-Performing Adsorbents for the Removal of Dissolved and Colloidal Substances

  • Liangyi Yao,
  • Xiangyuan Zou,
  • Shuqi Zhou,
  • Hongxiang Zhu,
  • Guoning Chen,
  • Shuangfei Wang,
  • Xiuyu Liu and
  • Yan Jiang

24 February 2022

The accumulation of dissolved and colloidal substances (DCS) in the increasingly closed paper circulating water system can seriously lower the productivity and safety of papermaking machines, and it has been a challenge to develop an adsorbent with l...

  • Article
  • Open Access
3 Citations
1,512 Views
15 Pages

Multi-Functional Alginate Lyase AlgVR7 from Vibrio rumoiensis: Structural Insights and Catalytic Mechanisms

  • Zhe Huang,
  • Shuai Liang,
  • Wulong Jiang,
  • Li Wang,
  • Yuan Wang,
  • Hua Wang,
  • Lianshun Wang,
  • Yuting Cong,
  • Yanan Lu and
  • Guojun Yang

13 March 2025

In this study, we identified AlgVR7, a novel bifunctional alginate lyase from Vibrio rumoiensis and characterized its biochemical properties and substrate specificity. Sequence alignment analysis inferred the key residues K267, H162, N86, E189, and T...

  • Article
  • Open Access
3 Citations
2,581 Views
9 Pages

Characterization of Major Cell-Wall-Degrading Enzymes Secreted by Diaporthe spp. Isolate Z1-1N Causing Postharvest Fruit Rot in Kiwifruit in China

  • Li-Zhen Ling,
  • Ling-Ling Chen,
  • Jia-Yu Ma,
  • Chao-Yue Li,
  • Dong-Ru Zhang,
  • Xiao-Di Hu and
  • Shu-Dong Zhang

2 December 2024

Pathogen-induced fruit decay is a significant threat to the kiwifruit industry, leading to considerable economic losses annually. The cell-wall-degrading enzymes (CWDEs) secreted by these pathogens are crucial for penetrating the cell wall and access...

  • Article
  • Open Access
18 Citations
4,183 Views
19 Pages

Macrofungal Mediated Biosynthesis of Silver Nanoparticles and Evaluation of Its Antibacterial and Wound-Healing Efficacy

  • Gayathri Vijayakumar,
  • Hyung Joo Kim,
  • Jeong Wook Jo and
  • Senthil Kumaran Rangarajulu

Recently, the utilization of biological agents in the green synthesis of nanoparticles has been given interest. In this study, silver nanoparticles were synthesized from an aqueous extract of macrofungus (mushroom), namely Phellinus adamantinus,...

  • Review
  • Open Access
5 Citations
2,575 Views
54 Pages

Calorimetric (from both isothermal micro-calorimetry and DSC), chiro-optical, viscometric and rheological data on aqueous solutions of pectic acid and low-methoxyl pectin (LMP), published over decades from different laboratories, have been comparativ...