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  • Feature Paper
  • Review
  • Open Access
56 Citations
15,481 Views
52 Pages

Quinoa Phenotyping Methodologies: An International Consensus

  • Clara S. Stanschewski,
  • Elodie Rey,
  • Gabriele Fiene,
  • Evan B. Craine,
  • Gordon Wellman,
  • Vanessa J. Melino,
  • Dilan S. R. Patiranage,
  • Kasper Johansen,
  • Sandra M. Schmöckel and
  • on behalf of the Quinoa Phenotyping Consortium
  • + 18 authors

24 August 2021

Quinoa is a crop originating in the Andes but grown more widely and with the genetic potential for significant further expansion. Due to the phenotypic plasticity of quinoa, varieties need to be assessed across years and multiple locations. To improv...

  • Review
  • Open Access
951 Citations
46,887 Views
34 Pages

A Review of Imaging Techniques for Plant Phenotyping

  • Lei Li,
  • Qin Zhang and
  • Danfeng Huang

24 October 2014

Given the rapid development of plant genomic technologies, a lack of access to plant phenotyping capabilities limits our ability to dissect the genetics of quantitative traits. Effective, high-throughput phenotyping platforms have recently been devel...

  • Review
  • Open Access
25 Citations
4,253 Views
22 Pages

10 August 2023

In response to escalating global demand for poultry, the industry grapples with an array of intricate challenges, from enhancing productivity to improving animal welfare and attenuating environmental impacts. This comprehensive review explores the tr...

  • Article
  • Open Access
14 Citations
4,302 Views
11 Pages

EGPA Phenotyping: Not Only ANCA, but Also Eosinophils

  • Andrea Matucci,
  • Emanuele Vivarelli,
  • Margherita Perlato,
  • Valentina Mecheri,
  • Matteo Accinno,
  • Lorenzo Cosmi,
  • Paola Parronchi,
  • Oliviero Rossi and
  • Alessandra Vultaggio

Background: Eosinophilic Granulomatosis with Polyangiitis (EGPA) is a small-vessel necrotizing vasculitis. The anti-neutrophil cytoplasmic antibodies’ (ANCA) role in defining clinical EGPA phenotypes is well established. Although the role of eo...

  • Review
  • Open Access
266 Citations
19,498 Views
19 Pages

Root Phenotyping for Drought Tolerance: A Review

  • Allah Wasaya,
  • Xiying Zhang,
  • Qin Fang and
  • Zongzheng Yan

31 October 2018

Plant roots play a significant role in plant growth by exploiting soil resources via the uptake of water and nutrients. Root traits such as fine root diameter, specific root length, specific root area, root angle, and root length density are consider...

  • Article
  • Open Access
25 Citations
4,813 Views
22 Pages

10 December 2019

In grapevine breeding, loose grape bunch architecture is one of the most important selection traits, contributing to an increased resilience towards Botrytis bunch rot. Grape bunch architecture is mainly influenced by the berry number, berry size, th...

  • Feature Paper
  • Review
  • Open Access
33 Citations
20,559 Views
83 Pages

Phenotyping of Drosophila Melanogaster—A Nutritional Perspective

  • Virginia Eickelberg,
  • Kai Lüersen,
  • Stefanie Staats and
  • Gerald Rimbach

27 January 2022

The model organism Drosophila melanogaster was increasingly applied in nutrition research in recent years. A range of methods are available for the phenotyping of D. melanogaster, which are outlined in the first part of this review. The methods inclu...

  • Review
  • Open Access
35 Citations
6,558 Views
20 Pages

16 October 2020

Bipolar disorder (BD) is a complex neurobiological disorder characterized by a pathologic mood swing. Digital phenotyping, defined as the ‘moment-by-moment quantification of the individual-level human phenotype in its own environment’, re...

  • Review
  • Open Access
14 Citations
7,086 Views
56 Pages

‘There and Back Again’—Forward Genetics and Reverse Phenotyping in Pulmonary Arterial Hypertension

  • Emilia M. Swietlik,
  • Matina Prapa,
  • Jennifer M. Martin,
  • Divya Pandya,
  • Kathryn Auckland,
  • Nicholas W. Morrell and
  • Stefan Gräf

26 November 2020

Although the invention of right heart catheterisation in the 1950s enabled accurate clinical diagnosis of pulmonary arterial hypertension (PAH), it was not until 2000 when the landmark discovery of the causative role of bone morphogenetic protein rec...

  • Review
  • Open Access
107 Citations
16,304 Views
21 Pages

31 July 2014

The improvements in crop production needed to meet the increasing food demand in the 21st Century will rely on improved crop management and better crop varieties. In the last decade our ability to use genetics and genomics in crop science has been re...

  • Review
  • Open Access
8 Citations
5,355 Views
23 Pages

Phenotyping in Arabidopsis and Crops—Are We Addressing the Same Traits? A Case Study in Tomato

  • Paolo Korwin Krukowski,
  • Jan Ellenberger,
  • Simone Röhlen-Schmittgen,
  • Andrea Schubert and
  • Francesca Cardinale

27 August 2020

The convenient model Arabidopsis thaliana has allowed tremendous advances in plant genetics and physiology, in spite of only being a weed. It has also unveiled the main molecular networks governing, among others, abiotic stress responses. Through the...

  • Review
  • Open Access
20 Citations
6,082 Views
17 Pages

30 August 2020

Wheat was one of the first grain crops domesticated by humans and remains among the major contributors to the global calorie and protein budget. The rapidly expanding world population demands further enhancement of yield and performance of wheat. Phe...

  • Review
  • Open Access
62 Citations
11,339 Views
23 Pages

Image-Based High-Throughput Phenotyping in Horticultural Crops

  • Alebel Mekuriaw Abebe,
  • Younguk Kim,
  • Jaeyoung Kim,
  • Song Lim Kim and
  • Jeongho Baek

22 May 2023

Plant phenotyping is the primary task of any plant breeding program, and accurate measurement of plant traits is essential to select genotypes with better quality, high yield, and climate resilience. The majority of currently used phenotyping techniq...

  • Article
  • Open Access
2 Citations
1,683 Views
23 Pages

21 October 2024

As one of the world’s most crucial food crops, maize plays a pivotal role in ensuring food security and driving economic growth. The diversification of maize variety breeding is significantly enhancing the cumulative benefits in these areas. Pr...

  • Article
  • Open Access
7 Citations
2,985 Views
19 Pages

Relieving the Phenotyping Bottleneck for Grape Bunch Architecture in Grapevine Breeding Research: Implementation of a 3D-Based Phenotyping Approach for Quantitative Trait Locus Mapping

  • Florian Rist,
  • Florian Schwander,
  • Robert Richter,
  • Jennifer Mack,
  • Anna Schwandner,
  • Ludger Hausmann,
  • Volker Steinhage,
  • Reinhard Töpfer and
  • Katja Herzog

In viticulture, winemakers and the industry demand grape bunches that have a reduced degree of bunch compactness. The major aspect is that a loose bunch compactness reduces the risk of severe Botrytis bunch-rot infections. Grapevine breeders focus he...

  • Article
  • Open Access
57 Citations
9,921 Views
14 Pages

An Automated Field Phenotyping Pipeline for Application in Grapevine Research

  • Anna Kicherer,
  • Katja Herzog,
  • Michael Pflanz,
  • Markus Wieland,
  • Philipp Rüger,
  • Steffen Kecke,
  • Heiner Kuhlmann and
  • Reinhard Töpfer

26 February 2015

Due to its perennial nature and size, the acquisition of phenotypic data in grapevine research is almost exclusively restricted to the field and done by visual estimation. This kind of evaluation procedure is limited by time, cost and the subjectivit...

  • Article
  • Open Access
78 Citations
12,952 Views
15 Pages

9 June 2015

To increase the understanding of how the plant phenotype is formed by genotype and environmental interactions, simple and robust high-throughput plant phenotyping methods should be developed and considered. This would not only broaden the application...

  • Review
  • Open Access
350 Citations
26,882 Views
22 Pages

Root Traits and Phenotyping Strategies for Plant Improvement

  • Ana Paez-Garcia,
  • Christy M. Motes,
  • Wolf-Rüdiger Scheible,
  • Rujin Chen,
  • Elison B. Blancaflor and
  • Maria J. Monteros

15 June 2015

Roots are crucial for nutrient and water acquisition and can be targeted to enhance plant productivity under a broad range of growing conditions. A current challenge for plant breeding is the limited ability to phenotype and select for desirable root...

  • Article
  • Open Access
47 Citations
9,996 Views
18 Pages

Phenoliner: A New Field Phenotyping Platform for Grapevine Research

  • Anna Kicherer,
  • Katja Herzog,
  • Nele Bendel,
  • Hans-Christian Klück,
  • Andreas Backhaus,
  • Markus Wieland,
  • Johann Christian Rose,
  • Lasse Klingbeil,
  • Thomas Läbe and
  • Reinhard Töpfer
  • + 4 authors

14 July 2017

In grapevine research the acquisition of phenotypic data is largely restricted to the field due to its perennial nature and size. The methodologies used to assess morphological traits and phenology are mainly limited to visual scoring. Some measureme...

  • Review
  • Open Access
16 Citations
6,051 Views
24 Pages

17 November 2023

High-throughput phenotype monitoring systems for field crops can not only accelerate the breeding process but also provide important data support for precision agricultural monitoring. Traditional phenotype monitoring methods for field crops relying...

  • Article
  • Open Access
3 Citations
1,341 Views
21 Pages

Phenotyping in Green Lettuce Populations Through Multispectral Imaging

  • Jordhanna Marilia Silva,
  • Ana Carolina Pires Jacinto,
  • Ana Luisa Alves Ribeiro,
  • Isadora Rodrigues Damascena,
  • Livia Monteiro Ballador,
  • Paulo Henrique Lacerra,
  • Pablo Forlan Vargas,
  • George Deroco Martins and
  • Renata Castoldi

Lettuce (Lactuca sativa) is the most consumed leafy vegetable in the world, with great economic and social importance in Brazil. In breeding programs, selecting genotypes with high agronomic potential is essential to meet market demands and cultivati...

  • Review
  • Open Access
5 Citations
2,802 Views
28 Pages

13 April 2025

Animal phenotyping recognition is a pivotal component of precision livestock management, holding significant importance for intelligent farming practices and animal welfare assurance. In recent years, with the rapid advancement of deep learning techn...

  • Article
  • Open Access
18 Citations
6,531 Views
18 Pages

30 August 2021

Single-cell sequencing is a fast developing and very promising field; however, it is not commonly used in forensics. The main motivation behind introducing this technology into forensics is to improve mixture deconvolution, especially when a trace co...

  • Article
  • Open Access
3 Citations
2,630 Views
19 Pages

From Organelle Morphology to Whole-Plant Phenotyping: A Phenotypic Detection Method Based on Deep Learning

  • Hang Liu,
  • Hongfei Zhu,
  • Fei Liu,
  • Limiao Deng,
  • Guangxia Wu,
  • Zhongzhi Han and
  • Longgang Zhao

23 April 2024

The analysis of plant phenotype parameters is closely related to breeding, so plant phenotype research has strong practical significance. This paper used deep learning to classify Arabidopsis thaliana from the macro (plant) to the micro level (organe...

  • Article
  • Open Access
944 Views
22 Pages

10 December 2025

A one-size-fits-all approach to fall risk assessment in older adults has critical limitations. This study aimed to overcome this by identifying distinct gait phenotypes and their specific fall mechanisms using foot-mounted IMU waveform data from 146...

  • Feature Paper
  • Article
  • Open Access
41 Citations
9,783 Views
23 Pages

A Review of Automatic Phenotyping Approaches using Electronic Health Records

  • Hadeel Alzoubi,
  • Raid Alzubi,
  • Naeem Ramzan,
  • Daune West,
  • Tawfik Al-Hadhrami and
  • Mamoun Alazab

29 October 2019

Electronic Health Records (EHR) are a rich repository of valuable clinical information that exist in primary and secondary care databases. In order to utilize EHRs for medical observational research a range of algorithms for automatically identifying...

  • Review
  • Open Access
29 Citations
6,510 Views
23 Pages

Phenotyping Brown Rot Susceptibility in Stone Fruit: A Literature Review with Emphasis on Peach

  • Majid Hassan Mustafa,
  • Daniele Bassi,
  • Marie-Noëlle Corre,
  • Leandro Oliveira Lino,
  • Véronique Signoret,
  • Bénédicte Quilot-Turion and
  • Marco Cirilli

Plant disease phenotyping methodologies can vary considerably among testers and often suffer from shortcomings in their procedures and applications. This has been an important challenge in resistance breeding to brown rot, one of the most severe pre-...

  • Article
  • Open Access
6 Citations
3,125 Views
14 Pages

Phenotyping of Potato Plants Using Morphological and Physiological Tools

  • Olga Rozentsvet,
  • Elena Bogdanova,
  • Viktor Nesterov,
  • Alexey Bakunov,
  • Alexey Milekhin,
  • Sergei Rubtsov and
  • Victor Rozentsvet

26 February 2024

Potato (Solanum tuberosum L.) is one of the main non-grain agricultural crops and one of the main sources of food for humanity. Currently, growing potatoes requires new approaches and methods for cultivation and breeding. Phenotyping is one of the im...

  • Article
  • Open Access
3 Citations
2,548 Views
19 Pages

Development of a Quick-Install Rapid Phenotyping System

  • Roberto M. Buelvas,
  • Viacheslav I. Adamchuk,
  • John Lan,
  • Valerio Hoyos-Villegas,
  • Arlene Whitmore and
  • Martina V. Stromvik

25 April 2023

In recent years, there has been a growing need for accessible High-Throughput Plant Phenotyping (HTPP) platforms that can take measurements of plant traits in open fields. This paper presents a phenotyping system designed to address this issue by com...

  • Review
  • Open Access
38 Citations
5,799 Views
13 Pages

High-Throughput Phenotyping Methods for Breeding Drought-Tolerant Crops

  • Minsu Kim,
  • Chaewon Lee,
  • Subin Hong,
  • Song Lim Kim,
  • Jeong-Ho Baek and
  • Kyung-Hwan Kim

Drought is a main factor limiting crop yields. Modern agricultural technologies such as irrigation systems, ground mulching, and rainwater storage can prevent drought, but these are only temporary solutions. Understanding the physiological, biochemic...

  • Article
  • Open Access
11 Citations
5,607 Views
20 Pages

25 September 2023

Plant phenotyping has been widely studied as an effective and powerful tool for analyzing crop status and growth. However, the traditional phenotyping (i.e., manual) is time-consuming and laborious, and the various types of growing structures and lim...

  • Review
  • Open Access
228 Citations
19,736 Views
18 Pages

High-Throughput Field-Phenotyping Tools for Plant Breeding and Precision Agriculture

  • Aakash Chawade,
  • Joost van Ham,
  • Hanna Blomquist,
  • Oscar Bagge,
  • Erik Alexandersson and
  • Rodomiro Ortiz

High-throughput field phenotyping has garnered major attention in recent years leading to the development of several new protocols for recording various plant traits of interest. Phenotyping of plants for breeding and for precision agriculture have d...

  • Review
  • Open Access
1 Citations
1,919 Views
17 Pages

5 July 2023

Interethnic and interindividual variability in in vivo cytochrome P450 (CYP450)-dependent metabolism and altered drug absorption via expressed transport channels such as P-glycoprotein (P-gp) contribute to the adverse drug reactions, drug–drug...

  • Review
  • Open Access
24 Citations
4,969 Views
21 Pages

A Synthetic Review of Various Dimensions of Non-Destructive Plant Stress Phenotyping

  • Dapeng Ye,
  • Libin Wu,
  • Xiaobin Li,
  • Tolulope Opeyemi Atoba,
  • Wenhao Wu and
  • Haiyong Weng

18 April 2023

Non-destructive plant stress phenotyping begins with traditional one-dimensional (1D) spectroscopy, followed by two-dimensional (2D) imaging, three-dimensional (3D) or even temporal-three-dimensional (T-3D), spectral-three-dimensional (S-3D), and tem...

  • Article
  • Open Access
32 Citations
7,675 Views
21 Pages

Tomato Phenotypic Diversity Determined by Combined Approaches of Conventional and High-Throughput Tomato Analyzer Phenotyping

  • Amol N. Nankar,
  • Ivanka Tringovska,
  • Stanislava Grozeva,
  • Daniela Ganeva and
  • Dimitrina Kostova

5 February 2020

Morphological variation in vegetative and fruit traits is a key determinant in unraveling phenotypic diversity. This study was designed to assess phenotypic diversity in tomatoes and examine intra- and intervarietal groups’ variability using 28...

  • Article
  • Open Access
10 Citations
4,614 Views
12 Pages

Ultra-Wideband Microwave Imaging System for Root Phenotyping

  • Xiaodong Shi,
  • Jiaoyang Li,
  • Saptarshi Mukherjee,
  • Srijan Datta,
  • Vivek Rathod,
  • Xinyu Wang,
  • Wei Lu,
  • Lalita Udpa and
  • Yiming Deng

5 March 2022

The roots are a vital organ for plant growth and health. The opaque surrounding environment of the roots and the complicated growth process means that in situ and non-destructive root phenotyping face great challenges, which thus spur great research...

  • Article
  • Open Access
89 Citations
10,889 Views
22 Pages

13 December 2018

Phenotyping is the task of measuring plant attributes for analyzing the current state of the plant. In agriculture, phenotyping can be used to make decisions concerning the management of crops, such as the watering policy, or whether to spray for a c...

  • Article
  • Open Access
3 Citations
4,430 Views
15 Pages

OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision

  • Jiri Lindovsky,
  • Marcela Palkova,
  • Viktoriia Symkina,
  • Miles Joseph Raishbrook,
  • Jan Prochazka and
  • Radislav Sedlacek

22 January 2023

The purpose of the study is to demonstrate coherent optical tomography and electroretinography techniques adopted from the human clinical practice to assess the morphology and function of the mouse retina in a high-throughput phenotyping environment....

  • Review
  • Open Access
51 Citations
7,168 Views
20 Pages

Utilization of Spectral Indices for High-Throughput Phenotyping

  • Rupesh Tayade,
  • Jungbeom Yoon,
  • Liny Lay,
  • Abdul Latif Khan,
  • Youngnam Yoon and
  • Yoonha Kim

28 June 2022

The conventional plant breeding evaluation of large sets of plant phenotypes with precision and speed is very challenging. Thus, consistent, automated, multifaceted, and high-throughput phenotyping (HTP) technologies are becoming increasingly signifi...

  • Article
  • Open Access
10 Citations
6,792 Views
15 Pages

Low-Cost Automated Vectors and Modular Environmental Sensors for Plant Phenotyping

  • Stuart A. Bagley,
  • Jonathan A. Atkinson,
  • Henry Hunt,
  • Michael H. Wilson,
  • Tony P. Pridmore and
  • Darren M. Wells

11 June 2020

High-throughput plant phenotyping in controlled environments (growth chambers and glasshouses) is often delivered via large, expensive installations, leading to limited access and the increased relevance of “affordable phenotyping” soluti...

  • Communication
  • Open Access
1 Citations
2,817 Views
11 Pages

Retinal Phenotyping of a Murine Model of Lafora Disease

  • Ajoy Vincent,
  • Kashif Ahmed,
  • Rowaida Hussein,
  • Zorana Berberovic,
  • Anupreet Tumber,
  • Xiaochu Zhao and
  • Berge A. Minassian

31 March 2023

Lafora disease (LD) is a progressive neurologic disorder caused by biallelic pathogenic variants in EPM2A or EPM2B, leading to tissue accumulation of polyglucosan aggregates termed Lafora bodies (LBs). This study aimed to characterize the retinal phe...

  • Article
  • Open Access
1 Citations
1,069 Views
14 Pages

Genetic Diversity in Coffea canephora Genotypes via Digital Phenotyping

  • Priscila Sousa,
  • Henrique Vieira,
  • Eileen Santos,
  • Alexandre Viana and
  • Fábio Partelli

9 September 2025

C. canephora exhibits high genetic variability, and to estimate this variability, morphological descriptors associated with coffee quality are used. Bean size is a physical trait of great importance for coffee classification. Manual classification is...

  • Review
  • Open Access
2 Citations
2,269 Views
26 Pages

Applications of 3D Reconstruction Techniques in Crop Canopy Phenotyping: A Review

  • Yanzhou Li,
  • Zhuo Liang,
  • Bo Liu,
  • Lijuan Yin,
  • Fanghao Wan,
  • Wanqiang Qian and
  • Xi Qiao

29 October 2025

Amid growing challenges to global food security, high-throughput crop phenotyping has become an essential tool, playing a critical role in genetic improvement, biomass estimation, and disease prevention. Unlike controlled laboratory environments, fie...

  • Article
  • Open Access
40 Citations
7,119 Views
15 Pages

Novel 3D Imaging Systems for High-Throughput Phenotyping of Plants

  • Tian Gao,
  • Feiyu Zhu,
  • Puneet Paul,
  • Jaspreet Sandhu,
  • Henry Akrofi Doku,
  • Jianxin Sun,
  • Yu Pan,
  • Paul Staswick,
  • Harkamal Walia and
  • Hongfeng Yu

27 May 2021

The use of 3D plant models for high-throughput phenotyping is increasingly becoming a preferred method for many plant science researchers. Numerous camera-based imaging systems and reconstruction algorithms have been developed for the 3D reconstructi...

  • Review
  • Open Access
76 Citations
10,242 Views
25 Pages

27 October 2022

Computer vision (CV) combined with a deep convolutional neural network (CNN) has emerged as a reliable analytical method to effectively characterize and quantify high-throughput phenotyping of different grain crops, including rice, wheat, corn, and s...

  • Letter
  • Open Access
35 Citations
5,246 Views
10 Pages

9 February 2020

Enhancing plant breeding to ensure global food security requires new technologies. For wheat phenotyping, only limited seeds and resources are available in early selection cycles. This forces breeders to use small plots with single or multiple row pl...

  • Article
  • Open Access
2,747 Views
12 Pages

Initial Validation of a Behavioral Phenotyping Model for Substance Use Disorder

  • Lori Keyser-Marcus,
  • Tatiana Ramey,
  • James M. Bjork,
  • Caitlin E. Martin,
  • Roy Sabo and
  • F. Gerard Moeller

Standard nosological systems, such as DSM-5 or ICD-10, are relied upon as the diagnostic basis when developing treatments for individuals with substance use disorder (SUD). Unfortunately, the vast heterogeneity of individuals within a given SUD diagn...

  • Article
  • Open Access
29 Citations
11,731 Views
15 Pages

Novel Low Cost 3D Surface Model Reconstruction System for Plant Phenotyping

  • Suxing Liu,
  • Lucia M. Acosta-Gamboa,
  • Xiuzhen Huang and
  • Argelia Lorence

18 September 2017

Accurate high-resolution three-dimensional (3D) models are essential for a non-invasive analysis of phenotypic characteristics of plants. Previous limitations in 3D computer vision algorithms have led to a reliance on volumetric methods or expensive...

  • Article
  • Open Access
86 Citations
9,359 Views
19 Pages

Phenotyping of Corn Plants Using Unmanned Aerial Vehicle (UAV) Images

  • Wei Su,
  • Mingzheng Zhang,
  • Dahong Bian,
  • Zhe Liu,
  • Jianxi Huang,
  • Wei Wang,
  • Jiayu Wu and
  • Hao Guo

28 August 2019

Phenotyping provides important support for corn breeding. Unfortunately, the rapid detection of phenotypes has been the major limiting factor in estimating and predicting the outcomes of breeding programs. This study was focused on the potential of p...

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