The tumor microenvironment is a complex ecosystem containing various cell types, such as immune cells, fibroblasts, and endothelial cells, which interact with the tumor cells. In recent decades, the cancer research field has gained insight into the c...
Rapid multiplex cell surface marker analysis can expedite investigations in which large number of antigens need to be analyzed. Simultaneous analysis of multiple surface antigens at the same level of sensitivity is however limited in the current gold...
While the bone marrow attracts tumor cells in many solid cancers leading to poor outcome in affected patients, comprehensive analyses of bone marrow metastases have not been performed on a single-cell level. We here set out to capture tumor heterogen...
This paper studied the general mechanism of spatial-frequency-shift (SFS) super-resolution imaging based on multiplex illumination modulation. The theory of SFS joint intensity was first proposed. Experiments on parallel slots with discrete spatial f...
This paper introduces an innovative approach to integral volumetric imaging employing time and polarization multiplexing techniques to present volumetric three-dimensional images. Traditional integral volumetric imaging systems with a coarse lens arr...
To enhance imaging quality, singular value decomposition (SVD) has been applied to single-wavelength ghost imaging (GI) or color GI. In this paper, we extend the application of SVD to wavelength-multiplexing ghost imaging (WMGI) for reducing the redu...
Multiplexed imaging of Förster Resonance Energy Transfer (FRET)-based biosensors potentially presents a powerful approach to monitoring the spatio-temporal correlation of signalling pathways within a single live cell. Here, we discuss the potential o...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers. PDAC is characterized by a complex tumor microenvironment (TME), that plays a pivotal role in disease progression and resistance to therapy. Investigating the spatial distribu...
We propose an angle multiplexing method for optics-based image encryption using a phase-only computer-generated hologram (POCGH) in the tilted Fresnel transform (TFrT) domain. Modified Gerchberg-Saxton algorithms, based on the three types of rotation...
Targeting of the programmed cell death protein (PD-1)/programmed death-ligand 1 (PD-L1) axis with checkpoint inhibitors has changed clinical practice in non-small cell lung cancer (NSCLC). However, clinical assessment remains complex and ambiguous. W...
Spectral imaging detection using acousto-optical tunable filters (AOTFs) faces a significant challenge of low throughput due to the traditional design that only receives a single polarization light. To overcome this issue, we propose a novel polariza...
Since the discovery of antibiotics, the emergence of antibiotic resistance has become a global issue that is threatening society. In the era of antibiotic resistance, finding the proper antibiotics through antibiotic susceptibility testing (AST) is c...
High-throughput protein assays are crucial for modern diagnostics, drug discovery, proteomics, and other fields of biology and medicine. It allows simultaneous detection of hundreds of analytes and miniaturization of both fabrication and analytical p...
An approach is suggested to recover and then locate the original object image in off-axis digital holography by the multiplexing of hologram (OADHM). The recording configuration is designed by introducing one more plane wave in the interference frame...
Complex clinical procedures and small-animal research procedures can benefit from dual-site imaging provided by multiple endoscopic devices. Here, an endoscopic system is proposed which enables multiple fluorescence microendoscopes to be spectrally m...
Two-photon microscopy enables monitoring cellular dynamics and communication in complex systems, within a genuine environment, such as living tissues and, even, living organisms. Particularly, its application to understand cellular interactions in th...
A reversible data hiding in the encrypted domain (RDH-ED) aims to hide data within encrypted images, protecting its content, while allowing additional information to be distributed. This paper presents a complete separable RDH-ED scheme, whose main c...
Multiplex immunofluorescence (mIF) and digital image analysis (DIA) have transformed the ability to analyse multiple biomarkers. We aimed to validate a clinical workflow for quantifying PD-L1 in non-small cell lung cancer (NSCLC). NSCLC samples were...
The orthogonal frequency-division multiplexing (OFDM) transmission technique is well known to be efficient for data transmission but is susceptible to performance degradation due to factors such as high-order modulation schemes, multipath fading, and...
Ultrafast laser processing can produce micro/nanostructures, which is of great interest in advanced manufacturing. Ultrafast laser-induced events include non-equilibrium dynamic phenomena, occurring on the femtosecond to picosecond time scale and nan...
pH (low) insertion peptides (pHLIPs) have been developed for cancer imaging and therapy targeting the acidic extracellular microenvironment. However, the characteristics of intratumoral distribution (ITD) of pHLIPs are not yet fully understood. This...
We aimed to compare the geometric distortion (GD) correction performance and apparent diffusion coefficient (ADC) measurements of single-shot diffusion-weighted echo-planar imaging (SS-DWEPI), multiplexed sensitivity encoding (MUSE)-DWEPI, and MUSE-D...
Combined hepato-cholangiocarcinomas (cHCC-CCA) belong to the spectrum of primary liver carcinomas, which include hepatocellular carcinomas (HCC) and intrahepatic cholangiocarcinomas (iCCA) at both ends of the spectrum. Mainly due to the high intratum...
Immune checkpoint inhibitors, including those concerning programmed cell death 1 (PD-1) and its ligand (PD-L1), have revolutionised the cancer therapy approach in the past decade. However, not all patients benefit from immunotherapy equally. The pred...
Three-dimensional (3D) culture of tumor spheroids (TSs) within the extracellular matrix (ECM) represents a microtumor model that recapitulates human solid tumors in vivo, and is useful for 3D multiplex phenotypic analysis. However, the low efficiency...
Multiplexed positron emission tomography (PET) imaging provides perfectly registered simultaneous functional and molecular imaging of more than one biomarker. However, the separation of the multiplexed PET signals within a single PET scan is challeng...
Hyperspectral (HS) imaging involves the sensing of a scene’s spectral properties, which are often redundant in nature. The redundancy of the information motivates our quest to implement Compressive Sensing (CS) theory for HS imaging. This artic...
DNA point accumulation for imaging in nanoscale topography (PAINT) is a rapidly developing fluorescence super-resolution technique, which allows for reaching spatial resolutions below 10 nm. It also enables the imaging of multiple targets in the same...
Background/Objectives: Performing hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) on the same specimen slide provides advantages that include specimen conservation and the ability to combine the H&E context with biomarker...
Multiplexed platforms for multiple epitope detection have emerged in the last years as very powerful tools to study tumor tissues. These revolutionary technologies provide important visual techniques for tumor examination in formalin-fixed paraffin-e...
Background: Metastasis continues to be a leading cause of mortality in osteosarcoma (OS) among pediatric and young adult populations, with few effective therapeutic options available. Despite immunotherapy advancements, its efficacy in OS is hindered...
While label-free multiplex sensor technology enables “mixing and matching” of different capture molecules in principle, in practice this has been rarely (if ever) demonstrated. To fill this gap, we developed protocols for the preparation...
Diffraction-limited resolution and low penetration depth are fundamental constraints in optical microscopy and in vivo imaging. Recently, liquid-jet X-ray technology has enabled the generation of X-rays with high-power intensities in laboratory setti...
The tumor immune microenvironment (TIME) is an important player in breast cancer pathophysiology. Surrogates for antitumor immune response have been explored as predictive biomarkers to immunotherapy, though with several limitations. Immunohistochemi...
Technological breakthroughs have fundamentally changed our understanding on the complexity of the tumor microenvironment at the single-cell level. Characterizing the immune cell composition in relation to spatial distribution and histological changes...
Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD l...
To image 4-plex immunofluorescence-stained tissue samples at a low cost with cellular level resolution and sensitivity and dynamic range required to detect lowly and highly abundant targets, here we describe a robust, inexpensive (<$9000), 3D prin...
Genetically encoded fluorescent protein (FP)-based biosensors have revolutionized cell biology research by enabling real-time monitoring of molecular activities in live cells with exceptional spatial and temporal resolution. Multiplexed biosensing ad...
Although immune checkpoint inhibitors (ICIs) have significantly improved the oncological outcomes, about one-third of patients affected by clear cell renal cell carcinoma (ccRCC) still experience recurrence. Current prognostic algorithms, such as the...
Recent super-resolution imaging technologies enable tracing chromatin conformation with nanometer-scale precision at the single-cell level. They revealed, for example, that human chromosomes fold into a complex three-dimensional structure within the...
There is a growing trend towards the development of high resolution and multiplexing metasurface holograms. In this paper, we propose the reconstruction of polarization multiplexing terahertz (THz) holographic images based on transmissive metasurface...
Single-shot ultrafast multi-frame imaging technology plays a crucial role in the observation of laser-induced plasma. However, there are many challenges in the application of laser processing, such as technology fusion and imaging stability. To provi...
Background/Objectives: Triple-negative breast cancer (TNBC) exhibits high immune infiltration yet remains clinically aggressive. Although immune checkpoint blockade benefits a subset of patients, the molecular programs enabling concurrent immune acti...
Background: Mucopolysaccharidoses (MPSs) are rare lysosomal storage disorders characterized by multisystem involvement; notably, skeletal abnormalities known as dysostosis multiplex and varying degrees of central nervous system impairment. Accurate r...
Aimed at the problems of optical frequency combs, such as their large number of comb lines, their high flatness, and their lack of ease in generating, as well as the fact that the channelization efficiency of the scheme based on optical frequency com...
A new family of fluorescent thiophene and thienothiophene-containing squaraine dyes is described with tunable wavelengths that cover the absorption/emission range of 600–800 nm. The deep-red and near-infrared fluorescent compounds were easily p...
A large-aperture radar composed of a multiple-input-multiple-output (MIMO) planar array can complete 3D through-wall imaging (TWI), but the simultaneous work of the multiple transceiver channels leads to difficulties in designing the hardware. In eng...
Both light emitting diode (LED) characteristics for illumination and communication simultaneously have made visible light communication-orthogonal frequency division multiplexing (VLC-OFDM) a strong competitive to radio frequency (RF). In this junctu...
Immune complexity status in the TME has been linked to clinical outcomes in pancreatic ductal adenocarcinoma (PDAC) patients. TME assessments with current cell marker and cell density-based analyses do not identify the original phenotypes of single c...
Anthrax lethal factor (LF) is one of the enzymatic components of the anthrax toxin responsible for the pathogenic responses of the anthrax disease. The ability to screen multiplexed ligands against LF and subsequently estimate the effective kinetic r...
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