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39 Results Found

  • Article
  • Open Access
1,497 Views
16 Pages

Use of Relay Method for Enhancing Comb Construction by Apis cerana cerana Utilizing Apis mellifera ligustica

  • Shunhua Yang,
  • Shanglun Ge,
  • Yiqiu Liu,
  • Danyin Zhou,
  • Xueyang Gong and
  • Kun Dong

8 January 2025

Italian honey bees (IHBs, Apis mellifera ligustica) exhibit superior comb-building abilities compared with Chinese honey bees (CHBs, Apis cerana cerana), which often fail to fully utilize wax foundations, resulting in incomplete comb structures. The...

  • Article
  • Open Access
924 Views
15 Pages

Azoxystrobin and Picoxystrobin Lead to Decreased Fitness of Honey Bee Drones (Apis mellifera ligustica)

  • Wenlong Tong,
  • Lizhu Wang,
  • Bingfang Tao,
  • Huanjing Yao,
  • Huiping Liu,
  • Shaokang Huang,
  • Jianghong Li,
  • Xiaolan Xu and
  • Xinle Duan

Honey bees (Apis mellifera ligustica) are essential pollinators in both ecosystems and agricultural production. However, their populations are declining due to various factors, including pesticide exposure. Despite their importance, the reproductive...

  • Article
  • Open Access
1 Citations
1,998 Views
14 Pages

Monitoring Immune Modulation in Season Population: Identifying Effects and Markers Related to Apis mellifera ligustica Honey Bee Health

  • Olga Frunze,
  • Hyunjee Kim,
  • Byung-ju Kim,
  • Jeong-Hyeon Lee,
  • Mustafa Bilal and
  • Hyung-Wook Kwon

22 December 2023

Honey bees play a significant role in ecology, producing biologically active substances used to promote human health. However, unlike humans, the molecular markers indicating honey bee health remain unknown. Unfortunately, numerous reports of honey b...

  • Article
  • Open Access
903 Views
16 Pages

Honeybee olfaction can influence foraging behavior and affect crop pollination. Odor-binding proteins play a vital role in honeybee olfactory perception. A previous study based on the antennal transcriptome of Apis mellifera ligustica in melon and to...

  • Article
  • Open Access
5 Citations
2,848 Views
22 Pages

29 August 2023

This study aimed to assess the impact of oleic acid (OA) supplementation on the biosynthesis of 10-hydroxy-2-decenoic acid (10-HDA) in Apis mellifera ligustica. In experiment 1, varying concentrations of OA (2%, 4%, 6% and 8%) were added to an artifi...

  • Communication
  • Open Access
8 Citations
4,281 Views
10 Pages

Histopathological Findings in Testes from Apparently Healthy Drones of Apis mellifera ligustica

  • Karen Power,
  • Manuela Martano,
  • Gennaro Altamura and
  • Paola Maiolino

2 September 2020

It is well known that factors acting on the decrease of population of honeybees, can act on the male and female reproductive system, compromising the fertility of queens and drones. While there are many studies on female fertility, only a few studies...

  • Article
  • Open Access
25 Citations
5,250 Views
11 Pages

Microbial Profile of the Ventriculum of Honey Bee (Apis mellifera ligustica Spinola, 1806) Fed with Veterinary Drugs, Dietary Supplements and Non-Protein Amino Acids

  • Giovanni Cilia,
  • Filippo Fratini,
  • Elena Tafi,
  • Barbara Turchi,
  • Simone Mancini,
  • Simona Sagona,
  • Antonio Nanetti,
  • Domenico Cerri and
  • Antonio Felicioli

The effects of veterinary drugs, dietary supplements and non-protein amino acids on the European honey bee (Apis mellifera ligustica Spinola, 1806) ventriculum microbial profile were investigated. Total viable aerobic bacteria, Enterobacteriaceae, st...

  • Article
  • Open Access
1 Citations
4,084 Views
14 Pages

Two areas affected by cement plant emissions, in an industrial district of Central Italy, were investigated by foraging honeybees (Apis mellifera ligustica) on the return to their hives, as an in situ biomonitor. The contamination was compared with t...

  • Feature Paper
  • Article
  • Open Access
2,011 Views
19 Pages

Comparing Morphometric and Mitochondrial DNA Data from Honeybees and Honey Samples for Identifying Apis mellifera ligustica Subspecies at the Colony Level

  • Valeria Taurisano,
  • Anisa Ribani,
  • Valerio Joe Utzeri,
  • Dalal Sami,
  • Kate Elise Nelson Johnson,
  • Giovanni Formato,
  • Marcella Milito,
  • Giuseppina Schiavo,
  • Samuele Bovo and
  • Luca Fontanesi
  • + 1 author

12 June 2025

The ability to differentiate between different Apis mellifera subspecies can have significant implications for their conservation. In this study, we explored the possibility of obtaining multi-level information that could be useful in assigning a col...

  • Article
  • Open Access
4 Citations
3,062 Views
21 Pages

First Characterization and Regulatory Function of piRNAs in the Apis mellifera Larval Response to Ascosphaera apis Invasion

  • Minghui Sun,
  • Xiaoxue Fan,
  • Qi Long,
  • He Zang,
  • Yiqiong Zhang,
  • Xiaoyu Liu,
  • Peilin Feng,
  • Yuxuan Song,
  • Kunze Li and
  • Rui Guo
  • + 3 authors

15 November 2023

piRNAs are a class of small non-coding RNAs that play essential roles in modulating gene expression and abundant biological processes. To decode the piRNA-regulated larval response of western honeybees (Apis mellifera) to Ascosphaera apis infection,...

  • Article
  • Open Access
6 Citations
4,087 Views
20 Pages

Nutrition is a key aspect influencing honey bee health and overwintering. Since honey bee seasonality in southern temperate climates represents a significant research gap, this study conducted long-term monitoring of honey bees in the Mediterranean (...

  • Article
  • Open Access
40 Citations
4,349 Views
17 Pages

Inter- and Intra-Species Diversity of Lactic Acid Bacteria in Apis mellifera ligustica Colonies

  • Massimo Iorizzo,
  • Gianfranco Pannella,
  • Silvia Jane Lombardi,
  • Sonia Ganassi,
  • Bruno Testa,
  • Mariantonietta Succi,
  • Elena Sorrentino,
  • Sonia Petrarca,
  • Antonio De Cristofaro and
  • Patrizio Tremonte
  • + 1 author

Lactic acid bacteria could positively affect the health of honey bees, including nutritional supplementation, immune system development and pathogen colonization resistance. Based on these considerations the present study evaluated predominant Lactic...

  • Article
  • Open Access
11 Citations
2,602 Views
16 Pages

Bioaccumulation of Trace Elements along the Body Longitudinal Axis in Honey Bees

  • Enzo Goretti,
  • Matteo Pallottini,
  • Gianandrea La Porta,
  • Antonia Concetta Elia,
  • Tiziano Gardi,
  • Chiara Petroselli,
  • Paola Gravina,
  • Federica Bruschi,
  • Roberta Selvaggi and
  • David Cappelletti

7 June 2023

We present a survey on the environmental contamination of the Alviano Lake territory (Central Italy) based on Apis mellifera ligustica samples collected in two annual samplings (2019–2020). Concentrations of 30 elements were determined in the w...

  • Article
  • Open Access
44 Citations
5,685 Views
26 Pages

Genome-Wide Identification of Long Non-Coding RNAs and Their Regulatory Networks Involved in Apis mellifera ligustica Response to Nosema ceranae Infection

  • Dafu Chen,
  • Huazhi Chen,
  • Yu Du,
  • Dingding Zhou,
  • Sihai Geng,
  • Haipeng Wang,
  • Jieqi Wan,
  • Cuiling Xiong,
  • Yanzhen Zheng and
  • Rui Guo

9 August 2019

Long non-coding RNAs (lncRNAs) are a diverse class of transcripts that structurally resemble mRNAs but do not encode proteins, and lncRNAs have been proven to play pivotal roles in a wide range of biological processes in animals and plants. However,...

  • Communication
  • Open Access
1 Citations
4,394 Views
9 Pages

The mass rearing of high-quality queen bees is an essential beekeeping practice for producing new queens to maintain colony productivity. A strain of high royal-jelly-producing bees (RJBs; Apis mellifera ligustica) in China exhibits high potential fo...

  • Article
  • Open Access
3 Citations
1,633 Views
12 Pages

24 April 2024

Honeybees are prone to poisoning, also known as jujube flower disease, after collecting nectar from jujube flowers, resulting in the tumultuous demise of foragers. The prevalence of jujube flower disease has become one of the main factors affecting t...

  • Article
  • Open Access
3 Citations
1,790 Views
8 Pages

First Description of the Occurrence of Slow Bee Paralysis Virus-1 and Deformed Wing Virus B in Apis mellifera ligustica Honeybee in Italy

  • Eleonora Leti Maggio,
  • Silvia Tofani,
  • Anna Granato,
  • Giovanni Formato,
  • Gabriele Pietrella,
  • Raffaella Conti,
  • Marcella Milito,
  • Marco Pietropaoli,
  • Antonella Cersini and
  • Maria Teresa Scicluna

11 January 2024

Among the causes of bee colony death, viruses are among the number of pathogens that can contribute to the ill health of a colony. This study focuses on two of the several honeybee viruses, Slow Bee Paralysis Virus-1 (SBPV-1) and Deformed Wing Virus...

  • Article
  • Open Access
6 Citations
3,924 Views
15 Pages

Transcriptome-Wide Characterization of piRNAs during the Developmental Process of European Honey-Bee Larval Guts

  • Ya-Jing Xu,
  • Qi Long,
  • Xiao-Xue Fan,
  • Ya-Ping Ye,
  • Kai-Yao Zhang,
  • Jia-Xin Zhang,
  • Hao-Dong Zhao,
  • Yu-Tong Yao,
  • Zhong-Min Fu and
  • Zhe-Guang Lin
  • + 3 authors

17 October 2022

piRNAs play pivotal roles in maintaining genome stability, regulating gene expression, and modulating development and immunity. However, there are few piRNA-associated studies on honey-bees, and the regulatory role of piRNAs in the development of bee...

  • Article
  • Open Access
2 Citations
6,965 Views
13 Pages

16 October 2012

Unravelling the role of neuromessenger processes in learning and memory has long interested researchers. We investigated the effects of an acetylcholinesterase blocker, Methyl Parathion (MeP), on honeybee learning. We used visual and olfactory tasks...

  • Article
  • Open Access
6 Citations
4,175 Views
11 Pages

Geometric Morphometric Analysis of Wing Shape to Identify Populations of Apis mellifera in Camagüey, Cuba

  • Diego Masaquiza,
  • Mario Octavio Ferrán,
  • Santiago Guamán,
  • Edwin Naranjo,
  • Maritza Vaca,
  • Lino Marcelo Curbelo and
  • Amilcar Arenal

22 March 2023

A total of 45 Apis mellifera colonies were sampled from nine centers for rearing queens in the Camagüey province, Cuba. Wing geometric morphometric analysis was used to determine the ancestry and identify Africanization processes at different al...

  • Article
  • Open Access
43 Citations
6,986 Views
15 Pages

New Viruses from the Ectoparasite Mite Varroa destructor Infesting Apis mellifera and Apis cerana

  • Sofia Levin,
  • Noa Sela,
  • Tal Erez,
  • David Nestel,
  • Jeffery Pettis,
  • Peter Neumann and
  • Nor Chejanovsky

24 January 2019

Varroa destructor is an ectoparasitic mite of Asian or Eastern honeybees Apis cerana (A. cerana) which has become a serious threat to European subspecies of Western honeybees Apis mellifera (A. mellifera) within the last century. V. destructor and it...

  • Article
  • Open Access
8 Citations
5,069 Views
13 Pages

Whole-Genome Sequence Analysis of Italian Honeybees (Apis mellifera)

  • Giulietta Minozzi,
  • Barbara Lazzari,
  • Maria Grazia De Iorio,
  • Cecilia Costa,
  • Emanuele Carpana,
  • Paola Crepaldi,
  • Rita Rizzi,
  • Elena Facchini,
  • Gustavo Gandini and
  • Giulio Pagnacco
  • + 1 author

2 May 2021

At the end of the last glaciation, Apis mellifera was established in northern Europe. In Italy, Apis melliferaligustica adapted to the mild climate and to the rich floristic biodiversity. Today, with the spread of Varroa destructor and with the incre...

  • Article
  • Open Access
3 Citations
5,312 Views
20 Pages

The Carniolan Honeybee from Slovenia—A Complete and Annotated Mitochondrial Genome with Comparisons to Closely Related Apis mellifera Subspecies

  • Ajda Moškrič,
  • Andraž Marinč,
  • Polonca Ferk,
  • Brane Leskošek,
  • Mai-Britt Mosbech,
  • Ignas Bunikis,
  • Olga Vinnere Pettersson,
  • Lucile Soler and
  • Janez Prešern

22 April 2022

The complete mitochondrial genome of the Carniolan honeybee (Apis mellifera carnica) from Slovenia, a homeland of this subspecies, was acquired in two contigs from WGS data and annotated. The newly obtained mitochondrial genome is a circular closed l...

  • Article
  • Open Access
6 Citations
2,368 Views
12 Pages

Haplotypes of the tRNAleu-COII mtDNA Region in Russian Apis mellifera Populations

  • Milyausha D. Kaskinova,
  • Luisa R. Gaifullina and
  • Elena S. Saltykova

24 July 2023

Analysis of the mtDNA tRNAleu-COII locus is a widely used tool to establish belonging to a particular evolutionary lineage of Apis mellifera L. (lineages A, M, C, O, and Y). In Russia, most of the area was once inhabited by Apis mellifera mellifera f...

  • Article
  • Open Access
20 Citations
3,895 Views
18 Pages

Using the Software DeepWings© to Classify Honey Bees across Europe through Wing Geometric Morphometrics

  • Carlos Ariel Yadró García,
  • Pedro João Rodrigues,
  • Adam Tofilski,
  • Dylan Elen,
  • Grace P. McCormak,
  • Andrzej Oleksa,
  • Dora Henriques,
  • Rustem Ilyasov,
  • Anatoly Kartashev and
  • Maria Alice Pinto
  • + 2 authors

8 December 2022

DeepWings© is a software that uses machine learning to automatically classify honey bee subspecies by wing geometric morphometrics. Here, we tested the five subspecies classifier (A. m. carnica, Apis mellifera caucasia, A. m. iberiensis, Apis me...

  • Article
  • Open Access
8 Citations
5,333 Views
17 Pages

Unveiling the circRNA-Mediated Immune Responses of Western Honey Bee Larvae to Ascosphaera apis Invasion

  • Yaping Ye,
  • Xiaoxue Fan,
  • Zongbing Cai,
  • Ying Wu,
  • Wende Zhang,
  • Haodong Zhao,
  • Sijia Guo,
  • Peilin Feng,
  • Qiming Li and
  • Rui Guo
  • + 4 authors

29 December 2022

Western honey bee (Apis mellifera), a eusocial insect with a superior economic and ecological value, is widely used in the beekeeping industry throughout the world. As a new class of non-coding RNAs (ncRNAs), circular RNAs (circRNAs) participate in t...

  • Article
  • Open Access
2,407 Views
16 Pages

Varroa Volatiles Offer Chemical Cues to Honey Bees for Initial Parasitic Recognition

  • Qinglong Zhao,
  • Xinning Wang,
  • Ahsan Mustafa,
  • Ying Wang,
  • Hongfang Wang,
  • Xuepeng Chi,
  • Baohua Xu and
  • Zhenguo Liu

6 January 2025

Olfaction mediated by the antennae is a vital sensory modality for arthropods and could be applied as a tool in pest control. The ectoparasitic mite Varroa destructor poses a significant threat to the health of the honey bee Apis mellifera worldwide...

  • Article
  • Open Access
3 Citations
3,008 Views
16 Pages

Dynamic Evolution of Repetitive Elements and Chromatin States in Apis mellifera Subspecies

  • Nick Panyushev,
  • Max Selitskiy,
  • Vasilina Melnichenko,
  • Egor Lebedev,
  • Larisa Okorokova and
  • Leonid Adonin

11 January 2024

In this study, we elucidate the contribution of repetitive DNA sequences to the establishment of social structures in honeybees (Apis mellifera). Despite recent advancements in understanding the molecular mechanisms underlying the formation of honeyb...

  • Article
  • Open Access
4 Citations
2,473 Views
14 Pages

Investigations on Beekeeping and Breeding of Apis cerana in China

  • Xinying Qu,
  • Xinru Zhang,
  • Guiqian Zhang,
  • Hanrong Qin,
  • Huixia Zhang,
  • Huiyu Tian and
  • Xiao Chen

25 December 2024

The worldwide value of the honey bee as an agricultural animal is increasingly being recognized. Not only does the honey bee directly produce useful agricultural products, but also large portions of crops are dependent on the pollination activities o...

  • Article
  • Open Access
2,195 Views
15 Pages

Investigation and Study on the Biology and Morphology of Apis florea and Apis dorsata in Southern China

  • Xinying Qu,
  • Xinru Zhang,
  • Tian Sun,
  • Zequn Qiu,
  • Qihuang Lu,
  • Zhenghui Bi,
  • Hanrong Qin,
  • Junjun Hu,
  • Peng Tang and
  • Xiao Chen
  • + 1 author

21 February 2025

Honey bees are crucial pollinators that play a vital role in maintaining ecological balance. The colonies of managed honey bees in China increased rapidly in the past 20 years. Whether the rapid increase in managed bee populations would affect the su...

  • Article
  • Open Access
8 Citations
3,355 Views
13 Pages

Analysis of Complementary Sex-Determiner (csd) Allele Diversity in Different Honeybee Subspecies from Italy Based on NGS Data

  • Gianluigi Paolillo,
  • Maria Grazia De Iorio,
  • Joel F. Soares Filipe,
  • Federica Riva,
  • Alessandra Stella,
  • Gustavo Gandini,
  • Giulio Pagnacco,
  • Barbara Lazzari and
  • Giulietta Minozzi

31 May 2022

Sexual regulation in Apis mellifera is controlled by the complementary sex-determiner (csd) gene: females (queens and workers) are heterozygous at this locus and males (drones) are hemizygous. When homozygous diploid drones develop, they are eaten by...

  • Article
  • Open Access
3 Citations
3,252 Views
11 Pages

27 August 2021

The Western honeybee (Apis mellifera L.) is one of the most widespread insects with numerous subspecies in its native range. How far adaptation to local habitats has affected the cognitive skills of the different subspecies is an intriguing question...

  • Article
  • Open Access
8 Citations
3,047 Views
26 Pages

Deciphering the CircRNA-Regulated Response of Western Honey Bee (Apis mellifera) Workers to Microsporidian Invasion

  • Huazhi Chen,
  • Xiaoxue Fan,
  • Wende Zhang,
  • Yaping Ye,
  • Zongbing Cai,
  • Kaiyao Zhang,
  • Kuihao Zhang,
  • Zhongmin Fu,
  • Dafu Chen and
  • Rui Guo

29 August 2022

Vairimorpha ceranae is a widespread fungal parasite of adult honey bees that leads to a serious disease called nosemosis. Circular RNAs (circRNAs) are newly discovered non-coding RNAs (ncRNAs) that regulate biological processes such as immune defense...

  • Article
  • Open Access
8 Citations
6,148 Views
13 Pages

Horizontal Honey-Bee Larvae Rearing Plates Can Increase the Deformation Rate of Newly Emerged Adult Honey Bees

  • Juyeong Kim,
  • Kyongmi Chon,
  • Bo-Seon Kim,
  • Jin-A Oh,
  • Chang-Young Yoon,
  • Hong-Hyun Park and
  • Yong-Soo Choi

1 July 2021

Rearing honey bee larvae in vitro is an ideal method to study honey bee larval diseases or the toxicity of pesticides on honey bee larvae under standardized conditions. However, recent studies reported that a horizontal position may cause the deforma...

  • Article
  • Open Access
4 Citations
2,949 Views
13 Pages

Expression of Cry1Ab/2Aj Protein in Genetically Engineered Maize Plants and Its Transfer in the Arthropod Food Web

  • Yi Chen,
  • Michael Meissle,
  • Jiabao Xue,
  • Nan Zhang,
  • Shulin Ma,
  • Anping Guo,
  • Biao Liu,
  • Yufa Peng,
  • Xinyuan Song and
  • Yunhe Li
  • + 1 author

2 December 2023

While transgenic Bacillus thuringiensis (Bt) maize provides pest resistance and a reduced application of chemical pesticides, a comprehensive environmental risk assessment is mandatory before its field release. This research determined the concentrat...

  • Article
  • Open Access
1,166 Views
14 Pages

Honeybee Bioaccumulation as a Tool for Assessing the Environmental Quality of an Area Affected by the Activity of a Municipal Waste Sorting Facility (Central Italy)

  • Matteo Pallottini,
  • Enzo Goretti,
  • Tiziano Gardi,
  • Marco Petrarchini,
  • Aron Pazzaglia,
  • Beatrice Castellani,
  • Federica Bruschi,
  • Chiara Petroselli,
  • Roberta Selvaggi and
  • David Cappelletti

6 February 2025

The management of municipal solid waste presents a significant challenge for cities. Facilities dedicated to sorting, treating, and recycling waste (including plastic, glass, metals, aluminium, and wood) play a crucial environmental role in urban are...

  • Article
  • Open Access
4 Citations
2,883 Views
18 Pages

Honey bees are commonly used to study metabolic processes, yet the molecular mechanisms underlying nutrient transformation, particularly proteins and their effects on development, health, and diseases, still evoke varying opinions among researchers....

  • Article
  • Open Access
1 Citations
1,230 Views
14 Pages

Systemic Assessment of Chronic Toxicity of Thiamethoxam on Honeybees (Apis mellifera)

  • Meng-Jia Li,
  • Qi-Bao He,
  • Yi-Fan Wu,
  • Quan Gao,
  • A-Long Wang,
  • Jin-Jing Xiao,
  • Min Liao,
  • Yong Huang,
  • Yao-Hui Wang and
  • Hai-Qun Cao

5 September 2025

Honeybees (Apis mellifera ligustica Spin.) are the most significant pollinating insects, playing a vital role in maintaining biodiversity. In healthy colonies, the reproductive success of the queen and the genetic contribution of drones are essential...

  • Article
  • Open Access
2 Citations
2,942 Views
16 Pages

12 January 2023

The proboscis extension response (PER) assay revealed the responsiveness of three subspecies of the honeybee Apis mellifera [A. m. jemenitica (AMJ), A. m. carnica (AMC), and A. m. ligustica (AML)] to water and different concentrations (0.00001, 0.000...