Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom-up proteomic workflows consist of the following three major step...
Food samples such as milk, beverages, meat and chicken products, fish, etc. are complex and demanding matrices. Various novel materials such as molecular imprinted polymers (MIPs), carbon-based nanomaterials carbon nanotubes, graphene oxide and metal...
Covalent organic frameworks (COFs) can be classified as emerging porous crystalline polymers with extremely high porosity and surface area size, and good thermal stability. These properties have awakened the interests of many areas, opening new horiz...
Oxylipins are potent lipid mediators derived from polyunsaturated fatty acids, which play important roles in various biological processes. Being important regulators and/or markers of a wide range of normal and pathological processes, oxylipins are b...
Ionic liquids (ILs) are a group of non-conventional salts with melting points below 100 °C. Apart from their negligible vapor pressure at room temperature, high thermal stability, and impressive solvation properties, ILs are characterized by thei...
Obesity is associated with alterations in the composition and amounts of lipids. Lipids have over 1.7 million representatives. Most lipid groups differ in composition, properties and chemical structure. These small molecules control various metabolic...
Catecholamines (CAs) and their metabolites play significant roles in many physiological processes. Changes in CAs concentration in vivo can serve as potential indicators for the diagnosis of several diseases such as pheochromocytoma and paraganglioma...
The continuous growth in the production, unsustainable use, and disposal of plastics in recent decades has led to the emergence of a new type of pollutant, microplastics (MPs). In this article, the focus is on the form of MPs, which are produced by t...
In the past decade a large amount of analysis techniques have been scaled down to the microfluidic level. However, in many cases the necessary sample preparation, such as separation, mixing and concentration, remains to be performed off-chip. This re...
Clinical analyses benefit world-wide from rapid and reliable diagnostics tests. New tests are sought with greatest demand not only for new analytes, but also to reduce costs, complexity and lengthy analysis times of current techniques. Among the myri...
The escalating impact and remarkable progress of nanotechnology have shifted the paradigms of medicine and the healthcare system. Nanosystems have emerged, extensively holding the potential to advance disease diagnosis and treatment specificity. The...
To explore the influence of sample preparation methods on the permeability and microstructure of remolded loess, remolded loess collected from Heifangtai was taken as the research object. A total of 40 sets of falling-head permeability tests were con...
Sample preparation is the most critical step in proteomics as it directly affects the subset of proteins and peptides that can be reliably identified and quantified. Although a variety of efficient and reproducible sample preparation strategies have...
Hazard detection systems must be evaluated with appropriate test material concentrations under controlled conditions in order to accurately identify and quantify unknown residues commonly utilized in theater. The existing assortment of hazard referen...
Thin sections are fundamental tools in mineralogy, petrography, archaeometry, paleontology, and heritage science, allowing for the microscopic study of mineral assemblages, rock textures, ceramic fabrics, and fossil microstructures. Although thin-sec...
High-resolution laboratory-based thermal infrared spectroscopy is an up-and-coming tool in the field of geological remote sensing. Its spatial resolution allows for detailed analyses at centimeter to sub-millimeter scales. However, this increase in r...
Ferrofluids (FFs) constitute a type of tunable magnetic material, formed by magnetic nanoparticles suspended in a liquid carrier. The astonishing magnetic properties of these materials and their liquid nature have led to their extended use in differe...
Cryo-electron microscopy (Cryo-EM) has become a routine technology for resolving the structure of biological macromolecules due to the resolution revolution in recent years. The specimens are typically prepared in a very thin layer of vitrified ice s...
Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and b...
Cloud point extraction is a sample preparation technique that involves using surfactants that are not harmful to the environment. It is based on micelle formation in which the extracted compound is encapsulated in the hydrophobic core of the micelles...
Sample preparation is an essential step for nearly every type of biochemical analysis in use today. Among the most important of these analyses is the diagnosis of diseases, since their treatment may rely greatly on time and, in the case of infectious...
In the last decades, a myriad of materials has been synthesized and utilized for the development of sample preparation procedures. The use of their magnetic analogues has gained significant attention and many procedures have been developed using magn...
Bitumen is a major construction material that can emit harmful fumes when heated. These fumes pose health risks to workers and communities near construction projects or asphalt mixing plants. The chemical complexity of bitumen fumes and the increasin...
During the compaction of silty soil subgrade, different filling methods are adopted, which will significantly impact the subgrade performance, but few studies have been applied to quantify this influence. To explore the influence mechanism of dry den...
A large number of carbon-based nanomaterials has been investigated as sorbents in sample preparation, including fullerenes, carbon nanotubes, nanofibers, nanohorns and graphene, as well as their functionalized forms. Taking into account their propert...
Transmission Electron Microscopy (TEM) requires electron-transparent samples with thickness below 100 nm, and conventional preparation methods involving mechanical polishing followed by electropolishing or ion milling are time-consuming and prone to...
Color additives are widely used by the food industry to enhance the appearance, as well as the nutritional properties of a food product. However, some of these substances may pose a potential risk to human health, especially if they are consumed exce...
Environmental contamination by microplastics is closely associated with the widespread use of plastics. However, reliable assessment of this problem remains challenging because of the lack of fully harmonized definitions and considerable variability...
Untargeted metabolomics aims to provide a global picture of the metabolites present in the system under study. To this end, making a careful choice of sample preparation is mandatory to obtain reliable and reproducible biological information. In this...
Even though instrumental advancements are constantly being made in analytical chemistry, sample preparation is still considered the bottleneck of analytical methods. To this end, researchers are developing new sorbent materials to improve and replace...
Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to pr...
A rapid and online microvolume flow-through dialysis probe designed for sample preparation in the analysis of veterinary drug residues is introduced. This study addresses the need for efficient and green sample preparation methods that reduce chemica...
Hafnia-based ferroelectric (FE) thin films have received extensive attention in both academia and industry, benefitting from their outstanding scalability and excellent CMOS compatibility. Hafnia-based FE capacitors in particular have the potential t...
Plant metabolomics, lipidomics, ionomics, fluxomics, and peptidomics are essential approaches for exploring how plants respond to epigenetic, pathological, and environmental stimuli through comprehensive chemical profiling. Over the past decades, sig...
There is a growing interest in monitoring microplastics in the environment, corresponding to increased public concerns regarding their potential adverse effects on ecosystems. Monitoring microplastics in the environment is difficult due to the comple...
Therapeutic monoclonal antibodies (mAbs) are dominating the biopharmaceutical field due to the fact of their high specificity in the treatment of diverse diseases. Nevertheless, mAbs are very complex glycoproteins exhibiting several macro- and microh...
Histone-based chromatin organization enabled eukaryotic genome complexity. This epigenetic control mechanism allowed for the differentiation of stable gene-expression and thus the very existence of multicellular organisms. This existential role in bi...
The use of cosmetics and personal care products is increasing worldwide. Their high matrix complexity, together with the wide range of products currently marketed under different forms imply a challenge for their analysis, most of them requiring a sa...
NMR-based metabolomics investigations of human biofluids offer great potential to uncover new biomarkers. In contrast to protocols for sample collection and biobanking, procedures for sample preparation prior to NMR measurements are still heterogeneo...
Dental light-cured resins can undergo different degrees of polymerization when applied in vivo. When polymerization is incomplete, toxic monomers may be released into the oral cavity. The present study assessed the cytotoxicity of different materials...
Background/Objectives: LC–MS/MS-based Multi-Attribute Method (MAM) workflows are increasingly used to characterize biotherapeutic proteins using peptide mapping and site-specific monitoring of critical quality attributes (CQAs). Sample preparat...
Fabric phase sorptive extraction (FPSE) is an evolutionary sample preparation approach which was introduced in 2014, meeting all green analytical chemistry (GAC) requirements by implementing a natural or synthetic permeable and flexible fabric substr...
Whereas the characterization of nanomaterials using different analytical techniques is often highly automated and standardized, the sample preparation that precedes it causes a bottleneck in nanomaterial analysis as it is performed manually. Usually,...
Global mass spectrometry (MS)-based proteomic and phosphoproteomic studies of acute myeloid leukemia (AML) biomarkers represent a powerful strategy to identify and confirm proteins and their phosphorylated modifications that could be applied in diagn...
The use of highly toxic rocket fuel based on 1,1-dimethylhydrazine (UDMH) in many types of carrier rockets poses a threat to environment and human health associated with an ingress of UDMH into wastewater and natural reservoirs and its transformation...
Regulatory bodies have started to recognise the value of in vitro screening and metabolomics as two types of new approach methodologies (NAMs) for chemical risk assessments, yet few high-throughput in vitro toxicometabolomics studies have been report...
Development of new diagnostic platforms that incorporate lab-on-a-chip technologies for portable assays is driving the need for rapid, simple, low cost methods to prepare samples for downstream processing or detection. An important component of the s...
The study of the metabolome within tissues, organisms, cells or biofluids can be carried out by several bioanalytical techniques. Among them, nuclear magnetic resonance (NMR) is one of the principal spectroscopic methods. This is due to a sample rota...
Lateral flow immunoassays (LFAs) are widely used for on-site testing; however, their use for the rapid detection of plant viruses in the field is often limited by inconvenient sample preparation. Here, we present a new sampling method and a simplifie...
Thermal and enzymatic reactions can significantly change the tissue metabolomic content during the sample preparation. In this work, we evaluated the stability of metabolites in human whole blood, serum, and rat brain, as well as in metabolomic extra...
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