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Metabolites
Volume 10
Issue 2
10.3390/metabo10020054
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Article

General Guidelines for Sample Preparation Strategies in HR-µMAS NMR-based Metabolomics of Microscopic Specimens

by
Covadonga Lucas-Torres
1,*,
Thierry Bernard
1,
Gaspard Huber
1,
Patrick Berthault
1,
Yusuke Nishiyama
2,3,
Pancham S. Kandiyal
4,
Bénédicte Elena-Herrmann
4,
Laurent Molin
5,
Florence Solari
5,
Anne-Karine Bouzier-Sore
6 and
Alan Wong
1,*
1
NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay 91191 Gif-sur-Yvette, France
2
JEOL RESONANCE Inc., Musashino, Akishima, Tokyo 196-8558, Japan
3
RIKEN-JEOL Collaboration Center, Yokohama, Kanagawa 230-0045, Japan
4
Univ Grenoble Alpes, CNRS, INSERM, IAB, Allée des Alpes, 38000 Grenoble, France
5
Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U 1217, Institut NeuroMyoGène, Lyon, France
6
Centre de Résonance Magnétique des Systèmes Biologiques, CNRS-Université de Bordeaux, UMR5536 Bordeaux, France
*
Authors to whom correspondence should be addressed.
Metabolites 2020, 10(2), 54; https://doi.org/10.3390/metabo10020054
Received: 16 December 2019 / Revised: 22 January 2020 / Accepted: 28 January 2020 / Published: 30 January 2020
(This article belongs to the Special Issue Sample Preparation in Metabolomics)
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Abstract

The study of the metabolome within tissues, organisms, cells or biofluids can be carried out by several bioanalytical techniques. Among them, nuclear magnetic resonance (NMR) is one of the principal spectroscopic methods. This is due to a sample rotation technique, high-resolution magic angle spinning (HR-MAS), which targets the analysis of heterogeneous specimens with a bulk sample mass from 5 to 10 mg. Recently, a new approach, high-resolution micro-magic angle spinning (HR-μMAS), has been introduced. It opens, for the first time, the possibility of investigating microscopic specimens (<500 μg) with NMR spectroscopy, strengthening the concept of homogeneous sampling in a heterogeneous specimen. As in all bioanalytical approaches, a clean and reliable sample preparation strategy is a significant component in designing metabolomics (or -omics, in general) studies. The sample preparation for HR-μMAS is consequentially complicated by the μg-scale specimen and has yet to be addressed. This report details the strategies for three specimen types: biofluids, fluid matrices and tissues. It also provides the basis for designing future μMAS NMR studies of microscopic specimens. View Full-Text
Keywords: high-resolution magic angle spinning; NMR; microscopic samples; metabolomics; sample preparation high-resolution magic angle spinning; NMR; microscopic samples; metabolomics; sample preparation
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MDPI and ACS Style

Lucas-Torres, C.; Bernard, T.; Huber, G.; Berthault, P.; Nishiyama, Y.; Kandiyal, P.S.; Elena-Herrmann, B.; Molin, L.; Solari, F.; Bouzier-Sore, A.-K.; Wong, A. General Guidelines for Sample Preparation Strategies in HR-µMAS NMR-based Metabolomics of Microscopic Specimens. Metabolites 2020, 10, 54. https://doi.org/10.3390/metabo10020054

AMA Style

Lucas-Torres C, Bernard T, Huber G, Berthault P, Nishiyama Y, Kandiyal PS, Elena-Herrmann B, Molin L, Solari F, Bouzier-Sore A-K, Wong A. General Guidelines for Sample Preparation Strategies in HR-µMAS NMR-based Metabolomics of Microscopic Specimens. Metabolites. 2020; 10(2):54. https://doi.org/10.3390/metabo10020054

Chicago/Turabian Style

Lucas-Torres, Covadonga, Thierry Bernard, Gaspard Huber, Patrick Berthault, Yusuke Nishiyama, Pancham S. Kandiyal, Bénédicte Elena-Herrmann, Laurent Molin, Florence Solari, Anne-Karine Bouzier-Sore, and Alan Wong. 2020. "General Guidelines for Sample Preparation Strategies in HR-µMAS NMR-based Metabolomics of Microscopic Specimens" Metabolites 10, no. 2: 54. https://doi.org/10.3390/metabo10020054

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