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Modern Methods of Sample Preparation for the Analysis of Oxylipins in Biological Samples

1
Department of Pharmaceutical Biochemistry, Medical University of Gdansk, Debinki 1, 80-211 Gdansk, Poland
2
Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Nuno Neng
Molecules 2019, 24(8), 1639; https://doi.org/10.3390/molecules24081639
Received: 26 March 2019 / Revised: 12 April 2019 / Accepted: 17 April 2019 / Published: 25 April 2019
(This article belongs to the Special Issue Modern Sample Preparation Approaches for Separation Science)
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Abstract

Oxylipins are potent lipid mediators derived from polyunsaturated fatty acids, which play important roles in various biological processes. Being important regulators and/or markers of a wide range of normal and pathological processes, oxylipins are becoming a popular subject of research; however, the low stability and often very low concentration of oxylipins in samples are a significant challenge for authors and continuous improvement is required in both the extraction and analysis techniques. In recent years, the study of oxylipins has been directly related to the development of new technological platforms based on mass spectrometry (LC–MS/MS and gas chromatography–mass spectrometry (GC–MS)/MS), as well as the improvement in methods for the extraction of oxylipins from biological samples. In this review, we systematize and compare information on sample preparation procedures, including solid-phase extraction, liquid–liquid extraction from different biological tissues. View Full-Text
Keywords: sample preparation; oxylipins; protein precipitation; liquid–liquid extraction; solid-phase extraction; biological samples sample preparation; oxylipins; protein precipitation; liquid–liquid extraction; solid-phase extraction; biological samples
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Liakh, I.; Pakiet, A.; Sledzinski, T.; Mika, A. Modern Methods of Sample Preparation for the Analysis of Oxylipins in Biological Samples. Molecules 2019, 24, 1639.

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