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  • Article
  • Open Access

Forces exerted by cells due to their internal contractility play fundamental roles in a host of processes, including adhesion, migration, survival and differentiation. Traction force microscopy (TFM) enables the determination of forces exerted by cel...

  • Article
  • Open Access
46 Views
22 Pages

Estimation and Classification of Coffee Plant Water Potential Using Spectral Reflectance and Machine Learning Techniques

  • Deyvis Cabrini Teixeira Delfino,
  • Danton Diego Ferreira,
  • Margarete Marin Lordelo Volpato,
  • Vânia Aparecida Silva,
  • Renan Teixeira Delfino,
  • Christiano Sousa Machado de Matos and
  • Meline de Oliveira Santos
Biophysica2025, 5(4), 60;https://doi.org/10.3390/biophysica5040060 
(registering DOI)

4 December 2025

Water potential is an important indicator used to study water relations in plants, as it reflects the level of hydration in their tissues. There are different numerical variables that describe plant properties and can be acquired from leaf reflectanc...

  • Communication
  • Open Access
119 Views
7 Pages

Membrane Depth Measurements of E Protein by 2H ESEEM Spectroscopy in Lipid Bilayers

  • Andrew K. Morris,
  • Robert M. McCarrick and
  • Gary A. Lorigan

26 November 2025

A topological analysis was performed by taking ESEEM measurements of site-specifically labeled E protein from SARS-CoV-2. The intensity of deuterium modulation arising from either deuterated solvent or deuterated lipid acyl chains revealed exposure t...

  • Review
  • Open Access
279 Views
25 Pages

21 November 2025

Protein droplets exhibit complex self-assembly and deposition behaviors driven by evaporation, which has attracted increasing attention in recent years. Under evaporation, limited volume and locally concentrated protein solutions can undergo liquid&n...

  • Article
  • Open Access
269 Views
26 Pages

18 November 2025

We present a proof-of-concept study using a laser trapping (LT) approach to characterize hemoglobin variants through controlled dielectric breakdown of red blood cell membranes. Using a 1064 nm infrared laser, we analyzed 62 cells from each of four h...

  • Article
  • Open Access
367 Views
15 Pages

12 November 2025

Early and accurate diagnosis of Alzheimer’s disease (AD) is critical for patient outcomes yet presents a significant clinical challenge. This study evaluates the effectiveness of four machine learning models—Logistic Regression, Random Fo...

  • Article
  • Open Access
429 Views
15 Pages

10 November 2025

Fluorescent proteins find application as biocompatible, genetically encoded labels for visualization of living organisms tissues. Green fluorescent proteins (GFPs) are the most diverse, but proteins with red fluorescence have advantages, such as lowe...

  • Review
  • Open Access
559 Views
19 Pages

Druggable Ensembles of Aβ and Tau: Intrinsically Disordered Proteins Biophysics, Liquid–Liquid Phase Separation and Multiscale Modeling for Alzheimer’s

  • Kunal Bhattacharya,
  • Pukar Khanal,
  • Jagdish Chand,
  • Nongmaithem Randhoni Chanu,
  • Dibyajyoti Das and
  • Atanu Bhattacharjee

Alzheimer’s disease is driven by multiple molecular drivers, including the pathological behavior of two intrinsically disordered proteins, amyloid-β (Aβ) and tau, whose aggregation is regulated by sequence-encoded ensembles and liquid...

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Biophysica - ISSN 2673-4125