The accurate measurement of spheroid area and morphology is critical for the progression of the integration of 3D models in in vitro cancer research and is increasingly used to measure effective therapeutic efficacy of X-ray radiation. Current method...
Testicular metabolism can be non-invasively assessed using FDG-PET/CT, which provides insights into physiological and age-related changes. Understanding normal testicular FDG uptake is essential to distinguish between benign variation and pathologica...
Sarcopenia is a degenerative musculoskeletal condition recognised as the age-related decline in skeletal muscle mass, strength, and function. Traditional diagnostic methods are limited by cost, accessibility, and subjectivity. This study aimed to dev...
Lipid-based delivery systems (LDS), including lipid nanoparticles (LNPs) and liposomes, have become indispensable tools in modern biomedicine owing to their biocompatibility, capacity to encapsulate diverse therapeutic agents, and potential for targe...
Antimicrobial resistance represents not only a biological challenge but also a physicochemical limitation associated with antibiotic transport, membrane interaction, and local availability. In this preliminary study, a liposome-encapsulated doxycycli...
Cancer is the second leading cause of death worldwide, requiring more effective treatments. By-products from the white shrimp (Penaeus vannamei) are a promising source of bioactive compounds. Compounds with antiproliferative activity were isolated an...
Traditional drug design methods based on trial and error are costly and inefficient. The computational approach ToSS-MoDE (Topological Substructural Molecular Design) offers an alternative by linking molecular descriptors to biological activity. To d...
This retrospective planning study evaluated how arc number (AN) and control-point density (CP) affect VMAT quality, radiobiological endpoints, and workflow efficiency for locally advanced cervical cancer in a resource-conscious setting. Twenty-one pa...
Protein folding displays a very narrow range of stability energy as measured by the ΔG° value associated with the process of protein folding from the unfolded sequence of amino acids defining a particular protein. We have recently reported...
Ion channels are fundamental membrane proteins that mediate selective ion flow across biological membranes and thereby govern excitability, signaling, and homeostasis in virtually all cell types. Although channel function is determined by intrinsic s...
Neurodegenerative conditions such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and amyotrophic lateral sclerosis (ALS) are devastating disorders with the gradual loss of neurons and cognitive or motor disabilit...
Peroxiredoxin 6 (Prdx6) is a bifunctional antioxidant enzyme with glutathione peroxidase and phospholipase A2 activities that plays an essential role in cellular redox regulation. However, the modulation of Prdx6 activity by endogenous small metaboli...
Fish collagen is derived from processing residues of marine and freshwater fish (such as fish skin, scales, and bones), primarily composed of amino acids including glycine, proline, and hydroxyproline. It functions include maintaining tissue integrit...
Cancer is one of the leading causes of mortality worldwide, with breast and colon cancers being among the most common neoplasms in men and women, respectively. Despite significant advancements in treatment, there is a pressing need to enhance specifi...
Mitochondria serve as central hubs of cellular metabolism, integrating catabolic and anabolic pathways through the controlled exchange of metabolites across their membranes. Although mitochondrial transport of several metabolites has been well docume...
Thrombosis is a cardiovascular disease characterized by the pathological formation of a fibrin clot in blood vessels. Currently available fibrinolytic enzymes have some limitations, including severe side effects, high cost, short half-life, and low f...
ORF8 is the second most mutated protein in SARS-CoV-2. It can form oligomers such as trimers and can bind to the IL-17RA/RC receptor. To understand the possible role of ORF8 in SARS-CoV-2, the first step of this study involved predicting the ORF8 tri...
Here we present a retrospective, integrative review of the approaches and discoveries of our “collaboratory”, a meta-laboratory comprising cross-disciplinary collaborations across laboratories at fourteen different universities and clinic...
The glomerular filtration barrier poses a significant challenge for circulating proteins, with molecules below ~60–70 kDa facing rapid renal clearance. Endogenous proteins have evolved sophisticated evasion mechanisms including oligomerization,...
Cedrus atlantica wood tar (CAWT) is traditionally used as a medicinal product, especially in low- and middle-income countries. Despite its traditional use, scientific support for its efficacy remains limited. This study evaluated the biological prope...
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by memory loss, cognitive decline, and oxidative stress-driven neuronal damage. Catalase, a key antioxidant enzyme, plays a vital role in decomposing hydrogen pe...
Making advancements in Quantitative Structure-Activity Relationship (QSAR) modeling is crucial for predicting biological activities in new compounds. Traditional 2D-QSAR and 3D-QSAR methods often face challenges in terms of computational efficiency a...
We investigated the roles and regulation of contractile and sodium ion transporter proteins in the pathogenesis of diarrhea in the acute ulcerative colitis. Acute ulcerative colitis was induced in male Sprague-Dawley rats using dextran sulfate sodium...
Mechanobiology explores how physical forces and cellular mechanics influence biological processes. This field has experienced rapid growth, driven by advances in high-resolution imaging, micromechanical testing, and computational modeling. At the sam...
Forces exerted by cells due to their internal contractility play fundamental roles in a host of processes, including adhesion, migration, survival and differentiation. Traction force microscopy (TFM) enables the determination of forces exerted by cel...
Water potential is an important indicator used to study water relations in plants, as it reflects the level of hydration in their tissues. There are different numerical variables that describe plant properties and can be acquired from leaf reflectanc...
A rapid, colorimetric sensor for histamine detection is presented using citrate-stabilized gold nanoparticles enhanced with Cu2+ coordination. The sensing mechanism involves dual recognition: protonated histamine first adsorbs electrostatically onto...
A topological analysis was performed by taking ESEEM measurements of site-specifically labeled E protein from SARS-CoV-2. The intensity of deuterium modulation arising from either deuterated solvent or deuterated lipid acyl chains revealed exposure t...
Protein droplets exhibit complex self-assembly and deposition behaviors driven by evaporation, which has attracted increasing attention in recent years. Under evaporation, limited volume and locally concentrated protein solutions can undergo liquid&n...
We present a proof-of-concept study using a laser trapping (LT) approach to characterize hemoglobin variants through controlled dielectric breakdown of red blood cell membranes. Using a 1064 nm infrared laser, we analyzed 62 cells from each of four h...
The combination of antimicrobial agents with different mechanisms of action is an important step in the fight against drug-resistant microorganisms. In this study, the interaction of the lysozyme enzyme with ampicillin and colistin was investigated....
Early and accurate diagnosis of Alzheimer’s disease (AD) is critical for patient outcomes yet presents a significant clinical challenge. This study evaluates the effectiveness of four machine learning models—Logistic Regression, Random Fo...
Fluorescent proteins find application as biocompatible, genetically encoded labels for visualization of living organisms tissues. Green fluorescent proteins (GFPs) are the most diverse, but proteins with red fluorescence have advantages, such as lowe...
Alzheimer’s disease is driven by multiple molecular drivers, including the pathological behavior of two intrinsically disordered proteins, amyloid-β (Aβ) and tau, whose aggregation is regulated by sequence-encoded ensembles and liquid...
Innate immunity is the body’s first line of defense for mounting robust antiviral signaling. However, the role of cytoskeletal prestress, a hallmark of cellular mechanotransduction, in regulating innate immune pathways such as retinoic acid-ind...
Large-scale computer simulations were employed to investigate the conformational response of the spike protein components S1 and S2 using a coarse-grained model. Temperature was systematically varied to assess the balance between stabilizing residue&...
This study explored the use of physical methods, namely X-ray diffraction, atomic force microscopy, and energy-dispersive X-ray spectroscopy, to analyze the structure and composition of tear fluid desiccates. Tear samples were collected from patients...
Microencapsulation is a fundamental technology for protecting active compounds from environmental degradation by factors such as light, heat, and oxygen. This process significantly improves their stability, bioavailability, and shelf life by entrappi...
Human granulocyte colony-stimulating factor (hG-CSF) is primarily used to treat neutropenia induced by cancer chemotherapy and bone marrow transplantation. The current identification test for hG-CSF relies on Western blot (WB), a labor-intensive and...
Post-COVID-19 syndrome (PCS) is a multifactorial disorder comprising different subgroups. Our study aimed to investigate the longitudinal changes in retinal microcirculation in PCS patients. Eighty PCS patients were recruited at the Department of Oph...
A systematic review was conducted to assess the effects of microgravity and space radiation on astronauts’ oral health. This review aimed to determine if these conditions increase the risk of dental and periodontal diseases, identify pre-missio...
Myelin is a membranous structure critically important for human health. Historically, it was believed that myelin remained largely unchanged in the adult brain. However, recent research has shown that myelin is remarkably dynamic, capable of adjustin...
Humanized mouse models offer human-specific platforms for investigating complex host–pathogen interactions, addressing shortcomings of conventional preclinical models that often fail to replicate human immune responses accurately. This integrat...
Metabolic imaging is undergoing a fundamental transformation. Traditionally confined to static representations of metabolite distribution through modalities such as PET, MRS, and MSOT, imaging has offered only partial glimpses into the dynamic and sy...
Heme enzymes that bind and reduce O2 are susceptible to poisoning by NO. The high reactivity and affinity of NO for ferrous heme produces stable ferrous-NO complexes, which in theory should preclude O2 binding and turnover. However, NO inhibition is...
Medical physicists are transforming physiological measurements and electrophysiological applications by addressing challenges like motion artifacts and regulatory compliance through advanced signal processing, artificial intelligence (AI), and statis...
Cryo-electron microscopy single-particle analysis (cryo-EM SPA) has advanced three-dimensional protein structure determination, yet resolving intrinsically disordered proteins and regions (IDPs/IDRs) remains challenging due to conformational heteroge...
Organs-on-Chips (OoC) technology has begun to be considered a pragmatic tool for drug evaluation, offering researchers an opportunity to move beyond the less physiologically relevant animal models. OoCs are microfluidic structures that imitate the fu...
Type 2 diabetes (T2D) is a chronic metabolic disorder requiring effective management to avoid complications. Metformin is a first-line drug agent and is routinely prescribed for the control of glycemia, but its underlying dynamics are complicated and...
The overexpression of atypical protein kinase C-iota (PKC-ι) is a biomarker for carcinogenesis in various cell types, such as glioma, ovarian, renal, etc., manifesting as a potential drug target. In previous in vitro studies, ICA-1S and ICA-1T,...
