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  • Article
  • Open Access
7 Citations
3,854 Views
13 Pages

Variation in Shrimp Allergens: Place of Origin Effects on Food Safety Assessment

  • Ryley D. Dorney,
  • Elecia B. Johnston,
  • Shaymaviswanathan Karnaneedi,
  • Thimo Ruethers,
  • Sandip D. Kamath,
  • Karthik Gopi,
  • Debashish Mazumder,
  • Jesmond Sammut,
  • Dean Jerry and
  • Andreas L. Lopata
  • + 2 authors

Due to the widespread use of shellfish ingredients in food products, accurate food labelling is urgently needed for consumers with shellfish allergies. Most crustacean allergen detection systems target the immunorecognition of the allergenic protein...

  • Review
  • Open Access
12 Citations
5,167 Views
21 Pages

Genomics of Shrimp Allergens and Beyond

  • Shanshan Li,
  • Ka Hou Chu and
  • Christine Yee Yan Wai

27 November 2023

Allergy to shellfishes, including mollusks and crustaceans, is a growing health concern worldwide. Crustacean shellfish is one of the “Big Eight” allergens designated by the U.S. Food and Drug Administration and is the major cause of food...

  • Review
  • Open Access
29 Citations
7,187 Views
22 Pages

IgE-Mediated Shellfish Allergy in Children

  • Mattia Giovannini,
  • Burcin Beken,
  • Betul Buyuktiryaki,
  • Simona Barni,
  • Giulia Liccioli,
  • Lucrezia Sarti,
  • Lorenzo Lodi,
  • Matteo Pontone,
  • Irene Bartha and
  • Antonella Muraro
  • + 4 authors

11 June 2023

Shellfish, including various species of mollusks (e.g., mussels, clams, and oysters) and crustaceans (e.g., shrimp, prawn, lobster, and crab), have been a keystone of healthy dietary recommendations due to their valuable protein content. In parallel...

  • Article
  • Open Access
6 Citations
3,145 Views
23 Pages

Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)

  • Emily M. Jerry,
  • Shaymaviswanathan Karnaneedi,
  • Thimo Ruethers,
  • Dean R. Jerry,
  • Kelly Condon and
  • Andreas L. Lopata

19 January 2024

Shellfish allergy affects ~2.5% of the global population and is a type I immune response resulting from exposure to crustacean and/or molluscan proteins. The Australian Redclaw crayfish (Cherax quadricarinatus) is a freshwater species endemic to and...

  • Review
  • Open Access
17 Citations
9,475 Views
16 Pages

IgE Mediated Shellfish Allergy in Children—A Review

  • Arianna Giannetti,
  • Andrea Pession,
  • Irene Bettini,
  • Giampaolo Ricci,
  • Giuliana Giannì and
  • Carlo Caffarelli

12 July 2023

Shellfish is a leading cause of food allergy and anaphylaxis worldwide. Recent advances in molecular characterization have led to a better understanding of the allergen profile. High sequence homology between shellfish species and between shellfish a...

  • Article
  • Open Access
7 Citations
3,112 Views
14 Pages

Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR

  • Mirjana Radomirović,
  • Nikola Gligorijević,
  • Dragana Stanić-Vučinić,
  • Andreja Rajković and
  • Tanja Ćirković Veličković

21 October 2023

Tropomyosin is the major and predominant allergen among shellfish. This study developed an ultrasensitive immuno-PCR method for the quantification of crustacean tropomyosin in foods. The method couples sandwich ELISA with the real-time PCR (rtPCR) am...

  • Article
  • Open Access
10 Citations
4,651 Views
14 Pages

Recombinant Tropomyosin from the Pacific Oyster (Crassostrea gigas) for Better Diagnosis

  • Roni Nugraha,
  • Thimo Ruethers,
  • Aya C. Taki,
  • Elecia B. Johnston,
  • Shaymaviswanathan Karnaneedi,
  • Sandip D. Kamath and
  • Andreas L. Lopata

30 January 2022

The Pacific oyster is a commercially important mollusc and, in contrast to most other shellfish species, frequently consumed without prior heat treatment. Oysters are rich in many nutrients but can also cause food allergy. Knowledge of their allergen...

  • Article
  • Open Access
38 Citations
4,946 Views
17 Pages

Effects of Extraction Buffer on the Solubility and Immunoreactivity of the Pacific Oyster Allergens

  • Roni Nugraha,
  • Thimo Ruethers,
  • Elecia B. Johnston,
  • Jennifer M. Rolland,
  • Robyn E. O’Hehir,
  • Sandip D. Kamath and
  • Andreas L. Lopata

12 February 2021

Despite recent technological advances, novel allergenic protein discovery is limited by their low abundance, often due to specific physical characteristics restricting their recovery during the extraction process from various allergen sources. In thi...

  • Article
  • Open Access
2,441 Views
16 Pages

Clinical and Immunological Features in Limpet (Patella sp.) Allergy in Subtropical Areas: A New Trigger for Food Anaphylaxis

  • Elena Mederos-Luis,
  • Tania Galán,
  • María J. Martínez,
  • Ruperto González-Pérez,
  • Inmaculada Sánchez-Machín,
  • Fernando Pineda and
  • Paloma Poza-Guedes

Seafood is a crucial source of nutrients, with global consumption steadily increasing. Among seafood-related allergies, shellfish are a significant cause of food allergy and anaphylaxis worldwide, affecting approximately 0.5–2.5% of the general...

  • Review
  • Open Access
1 Citations
2,346 Views
17 Pages

Shellfish Allergy Immunotherapy: Are We Moving Forward?

  • Lucio H. T. Fung,
  • Ho Lam Yeung,
  • Chun Wai Lim,
  • Shan Jiang,
  • Nicki Y. H. Leung,
  • Patrick S. C. Leung,
  • Ting Fan Leung and
  • Christine Y. Y. Wai

12 December 2025

Shellfish allergy is among the most common food allergies (FAs) worldwide and represents a severe immunoglobulin E (IgE)-mediated FA with tropomyosin functioning as the predominant pan-allergen. Current management of shellfish allergies is strictly p...

  • Article
  • Open Access
31 Citations
6,353 Views
25 Pages

Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species

  • Shaymaviswanathan Karnaneedi,
  • Roger Huerlimann,
  • Elecia B. Johnston,
  • Roni Nugraha,
  • Thimo Ruethers,
  • Aya C. Taki,
  • Sandip D. Kamath,
  • Nicholas M. Wade,
  • Dean R. Jerry and
  • Andreas L. Lopata

Shellfish allergy affects 2% of the world’s population and persists for life in most patients. The diagnosis of shellfish allergy, in particular shrimp, is challenging due to the similarity of allergenic proteins from other invertebrates. Despi...

  • Review
  • Open Access
66 Citations
14,900 Views
18 Pages

Overcoming Shellfish Allergy: How Far Have We Come?

  • Christine Y.Y. Wai,
  • Nicki Y.H. Leung,
  • Ka Hou Chu,
  • Patrick S.C. Leung,
  • Agnes S.Y. Leung,
  • Gary W.K. Wong and
  • Ting Fan Leung

Shellfish allergy caused by undesirable immunological responses upon ingestion of crustaceans and mollusks is a common cause of food allergy, especially in the Asia-Pacific region. While the prevalence of shellfish allergy is increasing, the mainstay...

  • Article
  • Open Access
43 Citations
7,504 Views
17 Pages

Insights into the Allergenic Potential of the Edible Yellow Mealworm (Tenebrio molitor)

  • Annick Barre,
  • Carole Pichereaux,
  • Esmeralda Velazquez,
  • Agathe Maudouit,
  • Mathias Simplicien,
  • Lorna Garnier,
  • Françoise Bienvenu,
  • Jacques Bienvenu,
  • Odile Burlet-Schiltz and
  • Pierre Rougé
  • + 2 authors

18 October 2019

The edible yellow mealworm (Tenebrio molitor), contains an extremely diverse panel of soluble proteins, including proteins with structural functions such as muscle proteins, as well as proteins involved in metabolic functions such as enzymes. Most of...

  • Review
  • Open Access
14 Citations
6,847 Views
15 Pages

Food Allergies: Immunosensors and Management

  • Dyah Ika Krisnawati,
  • Moh Alimansur,
  • Didik Susetiyanto Atmojo,
  • Elfi Quyumi Rahmawati,
  • Dwi Rahayu,
  • Erna Susilowati and
  • Tsung-Rong Kuo

25 February 2022

Food allergies (FA) are commonly depicted as immune responses. The mechanism of allergic reactions involves immunoglobulin E (IgE) and non-immunoglobulin E (non-IgE)-related responses caused by contact with specific foods. FAs can be fatal, have nega...

  • Review
  • Open Access
6 Citations
2,994 Views
15 Pages

Gastropod Allergy: A Comprehensive Narrative Review

  • Elena Mederos-Luis,
  • Paloma Poza-Guedes,
  • Fernando Pineda,
  • Inmaculada Sánchez-Machín and
  • Ruperto González-Pérez

Food allergies have increased significantly in recent decades, with shellfish being a leading cause of food allergy and anaphylaxis worldwide, affecting both children and adults. The prevalence of shellfish allergies is estimated to be approximately...

  • Review
  • Open Access
4 Citations
3,671 Views
34 Pages

An Overview of Seafood Allergens: Structure–Allergenicity Relationship and Allergenicity Elimination Processing Techniques

  • Yang Yang,
  • Yehao Zhang,
  • Xinrong He,
  • Fei Huan,
  • Jinli Chen,
  • Meng Liu,
  • Siyang He,
  • Shinong Gu and
  • Guangming Liu

25 June 2025

Seafood (fish, crustacean, and mollusk) allergy represents a critical global health issue. Food processing offers a viable strategy for allergenicity mitigation and serves as a critical intervention for seafood allergy prevention. This paper reviews...

  • Review
  • Open Access
14 Citations
5,728 Views
19 Pages

9 September 2022

Seafood allergy, mainly induced by fish, shrimp, crab, and shellfish, is a food safety problem worldwide. The non-thermal processing technology provides a new method in reducing seafood allergenicity. Based on the structural and antigenic properties...

  • Review
  • Open Access
21 Citations
8,227 Views
16 Pages

25 March 2024

Reaction to food allergens is on the increase and so is the attending cost on consumers, the food industry, and society at large. According to FDA, the “big-eight” allergens found in foods include wheat (gluten), peanuts, egg, shellfish,...

  • Review
  • Open Access
1 Citations
1,893 Views
22 Pages

15 September 2025

Food allergy is a growing global health concern, with seafood representing one of the most significant sources of allergic reactions. The primary allergens responsible for fish and shellfish allergies are β-parvalbumins and tropomyosin, respecti...

  • Review
  • Open Access
82 Citations
15,741 Views
30 Pages

Electrochemical Affinity Biosensors Based on Disposable Screen-Printed Electrodes for Detection of Food Allergens

  • Alina Vasilescu,
  • Gilvanda Nunes,
  • Akhtar Hayat,
  • Usman Latif and
  • Jean-Louis Marty

5 November 2016

Food allergens are proteins from nuts and tree nuts, fish, shellfish, wheat, soy, eggs or milk which trigger severe adverse reactions in the human body, involving IgE-type antibodies. Sensitive detection of allergens in a large variety of food matric...

  • Article
  • Open Access
2 Citations
2,371 Views
12 Pages

Multisyringe Flow Injection Analysis of Tropomyosin Allergens in Shellfish Samples

  • Bruno Coulomb,
  • Fabien Robert-Peillard,
  • Najib Ben Ali Gam,
  • Salwa Sadok and
  • Jean-Luc Boudenne

25 September 2021

This paper presents the development and the application of a multisyringe flow injection analysis system for the fluorimetric determination of the major heat-stable known allergen in shrimp, rPen a 1 (tropomyosin). This muscle protein, made up of 284...

  • Article
  • Open Access
24 Citations
6,143 Views
19 Pages

19 September 2019

Shellfish allergy is one of the most common food allergies, with tropomyosin as the major cross-reactive allergen. However, no allergen-specific immunotherapy is clinically available. Recently, we designed two shrimp hypoallergens MEM49 and MED171. T...

  • Review
  • Open Access
30 Citations
7,838 Views
14 Pages

18 August 2020

Seafood is considered one of the main food allergen sources by the European Food Safety Authority (EFSA). It comprises several distinct groups of edible aquatic animals, including fish and shellfish, such as crustacean and mollusks. Recently, the EFS...

  • Review
  • Open Access
33 Citations
7,296 Views
14 Pages

Advanced Proteomic and Bioinformatic Tools for Predictive Analysis of Allergens in Novel Foods

  • María López-Pedrouso,
  • José M. Lorenzo,
  • Juan de Dios Alché,
  • Ramón Moreira and
  • Daniel Franco

13 May 2023

In recent years, novel food is becoming an emerging trend increasingly more demanding in developed countries. Food proteins from vegetables (pulses, legumes, cereals), fungi, bacteria and insects are being researched to introduce them in meat alterna...

  • Article
  • Open Access
6 Citations
5,290 Views
11 Pages

Introduction of Egg White and Yolk to Infant Diets and Early Childhood Atopic Dermatitis

  • Man-Chin Hua,
  • Tsung-Chieh Yao,
  • Sui-Ling Liao,
  • Ming-Han Tsai,
  • Shen-Hao Lai,
  • Li-Chen Chen,
  • Kuan-Wen Su,
  • Chih-Yung Chiu,
  • Kuo-Wei Yeh and
  • Jing-Long Huang

13 March 2023

This study investigated whether the introduction of allergenic foods in infancy is associated with atopic dermatitis (AD) in early childhood. Information regarding parental allergic histories, the introduction of six possible allergenic foods (fruits...

  • Article
  • Open Access
12 Citations
3,694 Views
17 Pages

The Sensitization Profile for Selected Food Allergens in Polish Children Assessed with the Use of a Precision Allergy Molecular Diagnostic Technique

  • Izabela Knyziak-Mędrzycka,
  • Emilia Majsiak,
  • Weronika Gromek,
  • Danuta Kozłowska,
  • Jakub Swadźba,
  • Joanna Beata Bierła,
  • Ryszard Kurzawa and
  • Bożena Cukrowska

Individual populations show a variety of sensitization patterns, which may be associated with the geographic region, climate, dietary habits, or ways of preparing food. The purpose of this study was to comprehensively assess the food allergy sensitiz...

  • Article
  • Open Access
10 Citations
4,672 Views
12 Pages

Prevalence of Parent-Reported Food Allergies Among Children in Saudi Arabia

  • Ibrahim Alibrahim,
  • Maria AlSulami,
  • Turki Alotaibi,
  • Ruba Alotaibi,
  • Elaf Bahareth,
  • Inam Abulreish,
  • Sumayyah Alsuruji,
  • Imad Khojah,
  • Loie Goronfolah and
  • Amer Khojah
  • + 2 authors

14 August 2024

(1) Background: Food allergy (FA) is an immune-mediated hypersensitivity to foods, significantly contributing to childhood morbidity and mortality. This study aimed to assess the prevalence, characteristics, and influencing factors of parent-reported...

  • Article
  • Open Access
2 Citations
3,233 Views
9 Pages

Establishment of an Epicutaneously Sensitized Murine Model of Shellfish Allergy and Evaluation of Skin Condition by Raman Microscopy

  • Mayuko Ichimura-Shimizu,
  • Soichiro Ishimaru,
  • Christine (Yee-Yan) Wai,
  • Takeo Minamikawa,
  • Takaaki Tsunematsu,
  • Aiko Endo,
  • Takumi Kojima,
  • Minoru Matsumoto,
  • Tomoko Kobayashi and
  • Koichi Tsuneyama
  • + 5 authors

31 March 2022

Background: Shellfish allergy is one of the most common food allergies. Recent studies have shown that sensitization to allergens via the skin is involved in the development of food allergies. In this study, a mouse model of shrimp allergy was genera...

  • Article
  • Open Access
22 Citations
6,015 Views
13 Pages

29 September 2020

Optimal nutrition in early childhood fosters growth and development whilst preventing morbidity and mortality in later life. There is little research in New Zealand on commercially available complementary foods (CACFs). This cross-sectional study of...

  • Article
  • Open Access
14 Citations
2,652 Views
22 Pages

14 August 2023

Tropomyosin (TM) is a heat-stable protein that plays a crucial role as a major pan-allergen in crustacean shellfish. Despite the high thermal stability of the TM structure, its IgG/IgE binding ability, immunodetection, and in vitro digestibility can...

  • Article
  • Open Access
11 Citations
3,239 Views
11 Pages

Anaphylaxis after Shrimp Intake in a European Pediatric Population: Role of Molecular Diagnostics and Implications for Novel Foods

  • Michele Miraglia del Giudice,
  • Giulio Dinardo,
  • Angela Klain,
  • Elisabetta D’Addio,
  • Chiara Lucia Bencivenga,
  • Fabio Decimo and
  • Cristiana Indolfi

22 September 2023

(1) Background: Tropomyosin is a major cause of shellfish allergy and anaphylaxis triggered by food. It acts as a pan-allergen, inducing cross-reactivity in insects, dust mites, crustaceans, and mollusks. Our study investigates anaphylaxis in childre...

  • Article
  • Open Access
7 Citations
3,578 Views
12 Pages

4 October 2021

This study developed and characterized anti-fish monoclonal antibodies (mAbs) capable of detecting fish, a major allergenic food, in processed food products to protect fish sensitized individuals. Of the three mAbs raised against crude protein extrac...

  • Article
  • Open Access
860 Views
11 Pages

Adult Food Allergy Is an Under-Recognized Health Problem in Northwestern Mexico: A Population-Based Cross-Sectional Survey

  • Lizbeth Vizcarra-Olguin,
  • Marcela de Jesús Vergara-Jiménez,
  • Juancarlos Manuel Velásquez-Rodríguez,
  • Oscar Gerardo Figueroa-Salcido,
  • Elisa María Barrón-Cabrera,
  • Perla Y. Gutiérrez-Arzapalo,
  • Fernando Salas-López,
  • Noé Ontiveros and
  • Jesús Gilberto Arámburo-Gálvez

Objectives: To estimate the prevalence, clinical characteristics, and risk factors of IgE-mediated food allergy (FA) in adults from northwestern Mexico. Methods: A population-based, cross-sectional study was conducted in Culiacán, Sinaloa. A v...

  • Article
  • Open Access
1 Citations
3,253 Views
15 Pages

24 April 2023

Background: Allergy is known to be influenced by both diet and the immune system. In addition, the time of first exposure to food allergens and their type appear to play a particularly important role in the development of allergies. Aim: To determine...

  • Review
  • Open Access
964 Views
18 Pages

23 December 2025

The prevalence of food allergy continues to rise worldwide. The allergen profile of affected individuals changes with age: milk, egg, wheat, and soy allergies are more common in early childhood and often resolve before adolescence, whereas peanut, tr...

  • Article
  • Open Access
14 Citations
5,035 Views
15 Pages

21 April 2022

Tropomyosin in shellfish is considered a major cross-reactive allergen in house dust mites and cockroaches; however, the specific epitopes have not been elucidated. Therefore, this study aimed to identify the consensus antigenic determinant among shr...

  • Review
  • Open Access
48 Citations
10,285 Views
29 Pages

4 February 2022

The Food and Agriculture Organization of the United Nations (FAO) estimates that more than 500 million people, especially in Asia and Africa, are suffering from malnutrition. Recently, livestock farming has increased to supply high-quality protein, w...

  • Article
  • Open Access
23 Citations
58,537 Views
10 Pages

Prevalence of Self-Reported Food Allergies and Their Association with Other Health Conditions among Adults in Saudi Arabia

  • Nora A. Althumiri,
  • Mada H. Basyouni,
  • Norah AlMousa,
  • Mohammed F. AlJuwaysim,
  • Nasser F. BinDhim and
  • Saleh A. Alqahtani

Food allergies are a potentially life-threatening health issue, and few studies have determined their prevalence throughout Saudi Arabia. The main objective of our study was to estimate the prevalence and distribution of self-reported food allergies,...

  • Review
  • Open Access
3 Citations
4,086 Views
22 Pages

Transplant-Acquired Food Allergy in Children

  • Cristiana Indolfi,
  • Angela Klain,
  • Giulio Dinardo,
  • Carolina Grella,
  • Alessandra Perrotta,
  • Simone Colosimo,
  • Fabio Decimo and
  • Michele Miraglia del Giudice

22 September 2024

Background: Organ transplantation in children is a vital procedure for those with end-stage organ failure, but it has been linked to the development of post-transplant allergies, especially food allergies. This phenomenon, known as transplant-acquire...

  • Article
  • Open Access
1,773 Views
16 Pages

Integrated Diagnostic Approach Using Basophil Activation Test and IgE Assays for Shrimp and Prawn Allergy

  • Nhu N. Q. Nguyen,
  • Thao H. Nguyen,
  • Minh K. Le,
  • Tram B. Duong,
  • Duy L. Pham,
  • Tai T. Tran and
  • Tu H. K. Trinh

5 June 2025

Background and Objectives: Shellfish allergies are common in Vietnam. The basophil activation test (BAT) is a powerful tool in the diagnosis of food allergies. We aimed to evaluate the application of BAT to distinguish shrimp allergy in comparison wi...

  • Review
  • Open Access
1 Citations
2,946 Views
27 Pages

Sustainable Plant-Based Diets and Food Allergies: A Scoping Review Inspired by EAT-Lancet

  • Giuseppe Mazzola,
  • Carlo Cattaneo,
  • Eleonora Patta,
  • Tariq A. Alalwan,
  • Domenico Azzolino,
  • Simone Perna and
  • Mariangela Rondanelli

28 June 2025

Background: The escalating prevalence of food allergies, alongside the global call for environmentally sustainable dietary transitions, has drawn attention to plant-based dietary models—particularly those inspired by the EAT-Lancet Commission....