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Article

Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species

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Molecular Allergy Research Laboratory, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, QLD 4811, Australia
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Australian Institute of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia
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Centre for Food and Allergy Research, Murdoch Children’s Research Institute, The Royal Children’s Hospital, 50 Flemington Road, Parkville, VIC 3052, Australia
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ARC Research Hub for Advanced Prawn Breeding, Townsville, QLD 4811, Australia
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Centre for Sustainable Tropical Fisheries and Aquaculture, College of Science and Engineering, James Cook University, Townsville, QLD 4811, Australia
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Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, QLD 4811, Australia
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Department of Aquatic Product Technology, Bogor Agricultural University, Bogor 16680, Indonesia
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CSIRO Agriculture and Food, Aquaculture Program, 306 Carmody Road, St Lucia, QLD 4067, Australia
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Tropical Futures Institute, James Cook University, 149 Sims Drive, Singapore 387380, Singapore
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(1), 32; https://doi.org/10.3390/ijms22010032
Received: 19 November 2020 / Revised: 10 December 2020 / Accepted: 19 December 2020 / Published: 22 December 2020
(This article belongs to the Special Issue Molecular and Cellular Basis of Food Allergies)
Shellfish allergy affects 2% of the world’s population and persists for life in most patients. The diagnosis of shellfish allergy, in particular shrimp, is challenging due to the similarity of allergenic proteins from other invertebrates. Despite the clinical importance of immunological cross-reactivity among shellfish species and between allergenic invertebrates such as dust mites, the underlying molecular basis is not well understood. Here we mine the complete transcriptome of five frequently consumed shrimp species to identify and compare allergens with all known allergen sources. The transcriptomes were assembled de novo, using Trinity, from raw RNA-Seq data of the whiteleg shrimp (Litopenaeus vannamei), black tiger shrimp (Penaeus monodon), banana shrimp (Fenneropenaeus merguiensis), king shrimp (Melicertus latisulcatus), and endeavour shrimp (Metapenaeus endeavouri). BLAST searching using the two major allergen databases, WHO/IUIS Allergen Nomenclature and AllergenOnline, successfully identified all seven known crustacean allergens. The analyses revealed up to 39 unreported allergens in the different shrimp species, including heat shock protein (HSP), alpha-tubulin, chymotrypsin, cyclophilin, beta-enolase, aldolase A, and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Multiple sequence alignment (Clustal Omega) demonstrated high homology with allergens from other invertebrates including mites and cockroaches. This first transcriptomic analyses of allergens in a major food source provides a valuable resource for investigating shellfish allergens, comparing invertebrate allergens and future development of improved diagnostics for food allergy. View Full-Text
Keywords: allergy; prawn; tropomyosin; allergen; RNA-Seq allergy; prawn; tropomyosin; allergen; RNA-Seq
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MDPI and ACS Style

Karnaneedi, S.; Huerlimann, R.; Johnston, E.B.; Nugraha, R.; Ruethers, T.; Taki, A.C.; Kamath, S.D.; Wade, N.M.; Jerry, D.R.; Lopata, A.L. Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species. Int. J. Mol. Sci. 2021, 22, 32. https://doi.org/10.3390/ijms22010032

AMA Style

Karnaneedi S, Huerlimann R, Johnston EB, Nugraha R, Ruethers T, Taki AC, Kamath SD, Wade NM, Jerry DR, Lopata AL. Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species. International Journal of Molecular Sciences. 2021; 22(1):32. https://doi.org/10.3390/ijms22010032

Chicago/Turabian Style

Karnaneedi, Shaymaviswanathan, Roger Huerlimann, Elecia B. Johnston, Roni Nugraha, Thimo Ruethers, Aya C. Taki, Sandip D. Kamath, Nicholas M. Wade, Dean R. Jerry, and Andreas L. Lopata. 2021. "Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species" International Journal of Molecular Sciences 22, no. 1: 32. https://doi.org/10.3390/ijms22010032

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