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34 Results Found

  • Article
  • Open Access
5 Citations
7,875 Views
15 Pages

In Situ Random Microseeding and Streak Seeding Used for Growth of Crystals of Cold-Adapted β-d-Galactosidases: Crystal Structure of βDG from Arthrobacter sp. 32cB

  • Maria Rutkiewicz-Krotewicz,
  • Agnieszka J. Pietrzyk-Brzezinska,
  • Marta Wanarska,
  • Hubert Cieslinski and
  • Anna Bujacz

1 January 2018

There is an increasing demand for cold-adapted enzymes in a wide range of industrial branches. Nevertheless, structural information about them is still scarce. The knowledge of crystal structures is important to understand their mode of action and to...

  • Article
  • Open Access
2 Citations
3,947 Views
9 Pages

Biopurification of Oligosaccharides by Immobilized Kluyveromyces Lactis

  • In-Seok Yeo,
  • Yeo-Jin Yoon,
  • Nari Seo,
  • Hyun Joo An and
  • Jae-Han Kim

17 July 2019

Oligosaccharides with diverse and complex structures such as milk oligosaccharides have physiological functions including modulating intestinal microbiota or stimulating immune cell responses. However, milk carbohydrates include about 40–50% of...

  • Article
  • Open Access
1 Citations
3,113 Views
21 Pages

The influence of adding boric acid (BA) on the adsorption behavior of lactose onto an anion exchange resin (IRA402) was investigated. By adding BA, the amount of lactose adsorbed onto IRA402 was increased ca. 20% compared to without adding BA. In the...

  • Article
  • Open Access
5 Citations
6,055 Views
12 Pages

Altered Nutrient Composition of Lactose-Reduced Infant Formula

  • Pari Mokhtari,
  • Kelsey A. Schmidt,
  • Mahsa Babaei and
  • Michael I. Goran

17 January 2024

This research comprehensively examines 88 infant formulas available in the US market, with an emphasis on their diverging nutritional attributes based on lactose content. We stratified formulas into three categories: lactose-free, lactose-reduced, an...

  • Article
  • Open Access
8 Citations
2,767 Views
12 Pages

Effect of Lactose-Reduction in Murciano-Granadina Semi-Hard Goat Cheese on Physicochemical and Sensory Characteristics

  • Ángel Luis López Ruiz,
  • Francisco de Asís Ruiz Morales,
  • Pilar Ruiz Pérez-Cacho and
  • Hortensia Galán-Soldevilla

26 February 2023

Semi-hard pressed goat’s cheese, a traditional matured cheese in Andalusia, has a residual lactose content that may affect people with intolerance to that carbohydrate. Nowadays, lactose-free dairy products are characterized by presenting a sca...

  • Abstract
  • Open Access
2 Citations
3,807 Views
2 Pages

Daily Lactose Supplementation in Lactase Non-Persistent Individuals Induces Colonic Adaptation and Reduces Intolerance Symptoms

  • Ellen Looijesteijn,
  • Lonneke JanssenDuijghuijsen,
  • Maartje van den Belt,
  • Beatrix Gerhard,
  • Renata Ariens,
  • Reina Tjoelker and
  • Jan Geurts

Background and objectives: Globally, about 70% of the adult population is lactase non-persistent (LNP), lacking the enzyme required for lactose digestion. The main consequence of intolerance is withholding nutrient-rich dairy foods, while the literat...

  • Review
  • Open Access
14 Citations
11,060 Views
11 Pages

Much attention has recently been paid to β-Galactosidases (β-D-galactoside galactohidrolase; EC 3.2.1.23), commonly known as lactases, due to the lactose intolerance of the human population and the importance of dairy products in the human...

  • Article
  • Open Access
3 Citations
3,039 Views
11 Pages

Decolorization of Lactose-6-Phosphate Solutions Using Activated Carbon

  • Khalid A. Alsaleem,
  • Ahmed R. A. Hammam and
  • Lloyd E. Metzger

3 December 2023

Sugar phosphorylation has many applications that can be used to develop dairy and food products. During the phosphorylation process, the color of the solution turns into a dark color. The dark color causes many challenges and limitations in using pho...

  • Article
  • Open Access
14 Citations
6,271 Views
17 Pages

Evaluation of Binders in Twin-Screw Wet Granulation

  • Claudia Köster,
  • Sebastian Pohl and
  • Peter Kleinebudde

The binders povidone (Kollidon 30), copovidone (Kollidon VA64), hypromellose (Pharmacoat 606), and three types of hyprolose (HPC SSL-SFP, HPC SSL, and HPC SL-FP) were evaluated regarding their suitability in twin-screw wet granulation. Six mixtures o...

  • Article
  • Open Access
7 Citations
3,080 Views
20 Pages

23 September 2024

This study investigates the impact of substituting lactose with maltodextrin in milk–tea formulations to enhance their physicochemical and structural properties. Various lactose-to-maltodextrin ratios (100:0, 90:10, 85:15, 80:20, 75:25) were ev...

  • Article
  • Open Access
31 Citations
6,893 Views
23 Pages

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

  • Young-Cheol Chang,
  • Motakatla Venkateswar Reddy,
  • Kazuma Imura,
  • Rui Onodera,
  • Natsumi Kamada and
  • Yuki Sano

Cheese whey (CW) can be an excellent carbon source for polyhydroxyalkanoates (PHA)-producing bacteria. Most studies have used CW, which contains high amounts of lactose, however, there are no reports using raw CW, which has a relatively low amount of...

  • Feature Paper
  • Article
  • Open Access
4,013 Views
11 Pages

Acid Whey from Industrial Greek Strained Yoghurt: Effect of the Kind of Milk and the Way of Straining on Its Composition and Processing by Nanofiltration

  • Marianna Karela,
  • Lambros Sakkas,
  • Evangelia Zoidou,
  • Golfo Moatsou,
  • Konstantina Milosi and
  • Ekaterini Moschopoulou

28 April 2025

The acid whey derived during the production of Greek yoghurt, i.e., yoghurt acid whey (YAW), is considered as environmental pollutant due to its low pH and high lactose content. YAW may be obtained by centrifugation or ultrafiltration or filtration t...

  • Article
  • Open Access
4 Citations
2,482 Views
25 Pages

Single-Step Modification of Brewer’s Spent Grains Using Phosphoric Acid and Application in Cheese Whey Remediation via Liquid-Phase Adsorption

  • Luiz Eduardo Nochi Castro,
  • Larissa Resende Matheus,
  • Rosana Rabelo Mançano,
  • William Gustavo Sganzerla,
  • Rafael Gabriel da Rosa,
  • Tiago Linhares Cruz Tabosa Barroso,
  • Vanessa Cosme Ferreira and
  • Leda Maria Saragiotto Colpini

21 October 2023

Brewer’s spent grains (BSG) are a significant by-product of beer production, and its improper disposal poses environmental challenges. This study investigated the use of BSG for activated carbon production with phosphoric acid as a chemical act...

  • Article
  • Open Access
22 Citations
5,524 Views
16 Pages

27 August 2020

Galacto-oligosaccharides (GOS) are prebiotic compounds widely used for their health-promoting effects. Conventionally, GOS is produced by the enzymatic conversion of lactose in stirred tank reactors (STR). The high operational costs associated with e...

  • Article
  • Open Access
334 Views
22 Pages

Investigating the Separation Efficiency of Ultrafiltration/Diafiltration (UF/DF) of Whey by Dielectric Measurements

  • Réka Dobozi,
  • Zoltán Péter Jákói,
  • Sándor Beszédes,
  • Balázs P. Szabó and
  • Szabolcs Kertész

In whey valorization, membrane separation stands out as a highly effective technique for purifying and isolating the various components of whey. The efficiency of whey ultrafiltration and diafiltration (UF/DF) largely depends on the balance between m...

  • Article
  • Open Access
12 Citations
3,814 Views
14 Pages

Membrane Bioreactor for Simultaneous Synthesis and Fractionation of Oligosaccharides

  • Vanessa A. Botelho,
  • Marília Mateus,
  • José C. C. Petrus and
  • Maria Norberta de Pinho

31 January 2022

Galacto-oligosaccharides (GOS) are prebiotic sugars obtained enzymatically from lactose and used in food industry due to their nutritional advantages or technological properties. Selective mass transport and enzymatic synthesis were integrated and fo...

  • Article
  • Open Access
26 Citations
5,090 Views
17 Pages

Effects of Copper and pH on the Growth and Physiology of Desmodesmus sp. AARLG074

  • Nattaphorn Buayam,
  • Matthew P. Davey,
  • Alison G. Smith and
  • Chayakorn Pumas

Copper (Cu) is a heavy metal that is widely used in industry and as such wastewater from mining or industrial operations can contain high levels of Cu. Some aquatic algal species can tolerate and bioaccumulate Cu and so could play a key role in biore...

  • Article
  • Open Access
226 Views
15 Pages

Non-Thermal Milk Decontamination by Ionic Modulation: A Deionization-Based Alternative to Pasteurization

  • María T. Andrés,
  • Jessica González-Seisdedos,
  • Victoria Antuña and
  • José F. Fierro

21 January 2026

The dairy industry requires effective non-thermal processing strategies capable of ensuring microbial safety while preserving the nutritional and bioactive quality of milk. This study describes a novel milk decontamination approach based on selective...

  • Article
  • Open Access
20 Citations
5,156 Views
12 Pages

Optimization of 2-Phenylethanol Production from Sweet Whey Fermentation Using Kluyveromyces marxianus

  • Monserrat Alonso-Vargas,
  • Alejandro Téllez-Jurado,
  • Carlos A. Gómez-Aldapa,
  • María del Rocío Ramírez-Vargas,
  • Laura Conde-Báez,
  • Javier Castro-Rosas and
  • Arturo Cadena-Ramírez

The growing demand for natural products benefits the development of bioprocesses to obtain value-added compounds using residues such as sweet whey, which is rich in lactose. The yeast Kluyveromyces marxianus can ferment sweet whey to obtain 2-phenyle...

  • Article
  • Open Access
3 Citations
2,397 Views
12 Pages

A Low-Temperature and Low-Pressure Distillation Plant for Dairy Wastewater

  • Giuseppe Altieri,
  • Vincenzo De Luca,
  • Francesco Genovese,
  • Attilio Matera,
  • Luciano Scarano and
  • Giovanni Carlo Di Renzo

11 November 2022

The paper investigates an alternative treatment plant for the typical wastewater effluent of a cheese-making industry, mainly composed of acid or sweet whey mixed with washing-water. Two variable parameters have been considered during the tests: four...

  • Article
  • Open Access
4 Citations
3,766 Views
15 Pages

Effects of Random Mutagenesis and In Vivo Selection on the Specificity and Stability of a Thermozyme

  • Giuseppe Perugino,
  • Andrea Strazzulli,
  • Marialuisa Mazzone,
  • Mosè Rossi and
  • Marco Moracci

11 May 2019

Factors that give enzymes stability, activity, and substrate recognition result from the combination of few weak molecular interactions, which can be difficult to study through rational protein engineering approaches. We used irrational random mutage...

  • Article
  • Open Access
3 Citations
2,151 Views
14 Pages

13 October 2022

Acid whey is derived from fresh cheese. The proteins were isolated by a monolithic ion-exchange column. The remaining whey fraction was used as a starter culture substrate of kefir grains. The aim of this work was, firstly, to study the possibility o...

  • Article
  • Open Access
3 Citations
2,960 Views
13 Pages

Membrane Technology as a Strategy for Improving β-Galactosidase Concentration Processes: The Influence of the pH, Membrane Molecular Weight, Pressure, and Ionic Strength in the Process

  • Ailton Cesar Lemes,
  • Fabrício de Oliveira Molon,
  • Alexandre da Silva Fagundes,
  • Mariana Buranelo Egea,
  • Marco Di Luccio and
  • Susana Juliano Kalil

27 January 2023

The enzyme β-galactosidase catalyzes the hydrolysis of lactose into glucose and galactose, although for its effective application it is necessary to establish techniques for purification, concentration, or polishing, such as membrane separation...

  • Article
  • Open Access
9 Citations
2,324 Views
16 Pages

20 February 2024

The treatment of dairy industry effluents poses a significant challenge from the environmental point of view because of its high organic load. In this work, the aqueous phase reforming of lactose was investigated as a representative model compound fo...

  • Article
  • Open Access
1 Citations
3,557 Views
16 Pages

Impact of Carao (Cassia grandis) on Lactobacillus plantarum Immunomodulatory and Probiotic Capacity

  • Jhunior Marcia,
  • Hector Manuel Zumbado,
  • Manuel Álvarez Gil,
  • Daniel Martín-Vertedor,
  • Ismael Montero-Fernández,
  • Ajitesh Yadav and
  • Ricardo S. Aleman

Lactobacillus plantarum has beneficial effects on the reduction of symptoms of poor lactose digestion and hypercholesterolemia, removal of the duration and severity of diarrheal processes, improvement of the intestinal permeability barrier, preventio...

  • Article
  • Open Access
6 Citations
2,376 Views
19 Pages

Low-Frequency Vibrations of Saccharides Using Terahertz Time-Domain Spectroscopy and Ab-Initio Simulations

  • Andreea Aura Paraipan,
  • Nicole Luchetti,
  • Adriano Mosca Conte,
  • Olivia Pulci and
  • Mauro Missori

28 August 2023

The vibrational properties of molecular crystals in the terahertz range are controlled by the interplay of intermolecular and intramolecular interactions. The resulting delocalized normal modes span a substantial number of atoms within the unit cell...

  • Feature Paper
  • Article
  • Open Access
28 Citations
5,669 Views
14 Pages

A Three-Step Process for the Bioconversion of Whey Permeate into a Glucose-Free D-Tagatose Syrup

  • Fadia V. Cervantes,
  • Sawssan Neifar,
  • Zoran Merdzo,
  • Javier Viña-Gonzalez,
  • Lucia Fernandez-Arrojo,
  • Antonio O. Ballesteros,
  • Maria Fernandez-Lobato,
  • Samir Bejar and
  • Francisco J. Plou

9 June 2020

We have developed a sustainable three-stage process for the revaluation of cheese whey permeate into D-tagatose, a rare sugar with functional properties used as sweetener. The experimental conditions (pH, temperature, cofactors, etc.) for each step w...

  • Article
  • Open Access
13 Citations
2,859 Views
14 Pages

Characterization and Degradation Pathways of Microbacterium resistens MZT7, A Novel 17β-Estradiol-Degrading Bacterium

  • Peng Hao,
  • Sicheng Wu,
  • Xiqing Zhang,
  • Changlong Gou,
  • Yuqiong Wang,
  • Lixia Wang,
  • Yanbin Zhu,
  • Wangdui Basang and
  • Yunhang Gao

Due to the ecotoxicity of 17β-estradiol (E2), residual E2 in the environment poses potential risks to human and animal health and ecosystems. Biodegradation is considered one of the most effective strategies to remove E2 from the environment. He...

  • Article
  • Open Access
9 Citations
2,901 Views
19 Pages

Bovine Colostrum Supplementation Modulates the Intestinal Microbial Community in Rabbits

  • Stella Agradi,
  • Paola Cremonesi,
  • Laura Menchetti,
  • Claudia Balzaretti,
  • Marco Severgnini,
  • Federica Riva,
  • Bianca Castiglioni,
  • Susanna Draghi,
  • Alessia Di Giancamillo and
  • Gabriele Brecchia
  • + 8 authors

8 March 2023

BC is a nutraceutical that can modulate intestinal microbiota. This study investigates the effects of BC diet supplementation on luminal and mucosa-associated microbiota in the jejunum, caecum, and colon of rabbits. Twenty-one New Zealand White femal...

  • Article
  • Open Access
12 Citations
5,966 Views
10 Pages

7 December 2022

Greek strained yogurt is produced in high quantities worldwide. This production leaves behind acid whey, a by-product that is an environmental challenge. Hence, efforts are made to minimize the acid whey generation. In this study, the combined effect...

  • Article
  • Open Access
10 Citations
4,688 Views
13 Pages

Membrane filtration is a key technology in dairy processing for the separation of dairy liquids to clarify, concentrate, and fractionate a variety of dairy products. Ultrafiltration (UF) is widely applied for whey separation, protein concentration an...

  • Article
  • Open Access
3 Citations
197 Views

Qualitative Assessment of the Removable Denture Microbiome

  • Laura Iosif,
  • Ana Maria Cristina Țâncu,
  • Oana Elena Amza,
  • Bogdan Dimitriu,
  • Ana Ispas,
  • Mihaela Pantea and
  • Marina Imre

31 March 2024

Introduction: Assessment of the denture plaque can provide a valuable report regarding the oral health of geriatric patients and the oral hygiene habits in this population group. Focusing on the current research gap on this topic in Romania, our rese...

  • Article
  • Open Access
8 Citations
3,970 Views
23 Pages

Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation

  • Daniella Alejandra Pompa-Monroy,
  • Ana Leticia Iglesias,
  • Syed Gulam Dastager,
  • Meghana Namdeo Thorat,
  • Amelia Olivas-Sarabia,
  • Ricardo Valdez-Castro,
  • Lilia Angélica Hurtado-Ayala,
  • José Manuel Cornejo-Bravo,
  • Graciela Lizeth Pérez-González and
  • Luis Jesús Villarreal-Gómez

Augmenting bacterial growth is of great interest to the biotechnological industry. Hence, the effect of poly (caprolactone) fibrous scaffolds to promote the growth of different bacterial strains of biological and industrial interest was evaluated. Fu...

  • Article
  • Open Access
20 Citations
5,361 Views
13 Pages

The Effect of Whey on Performance, Gut Health and Bone Morphology Parameters in Broiler Chicks

  • Vasileios Tsiouris,
  • Michael G. Kontominas,
  • Giorgos Filioussis,
  • Sofia Chalvatzi,
  • Ilias Giannenas,
  • Georgios Papadopoulos,
  • Konstantinos Koutoulis,
  • Paschalis Fortomaris and
  • Ioanna Georgopoulou

5 May 2020

Whey is a highly nutritious byproduct of the cheese industry that can be used effectively in the animal feed industry. However, the use of whey in poultry diets is limited by its high lactose and mineral contents. The objective of the present study w...