Skip to Content

34 Results Found

  • Article
  • Open Access
4 Citations
3,965 Views
26 Pages

Full-Length Transcriptome Maps of Reef-Building Coral Illuminate the Molecular Basis of Calcification, Symbiosis, and Circadian Genes

  • Tingyu Han,
  • Xin Liao,
  • Yunchi Zhu,
  • Yunqing Liu,
  • Na Lu,
  • Yixin Li,
  • Zhuojun Guo,
  • J.-Y. Chen,
  • Chunpeng He and
  • Zuhong Lu

22 September 2022

Coral transcriptomic data largely rely on short-read sequencing, which severely limits the understanding of coral molecular mechanisms and leaves many important biological questions unresolved. Here, we sequence the full-length transcriptomes of four...

  • Article
  • Open Access
32 Citations
10,054 Views
26 Pages

Utilizing PacBio Iso-Seq for Novel Transcript and Gene Discovery of Abiotic Stress Responses in Oryza sativa L.

  • Stephanie Schaarschmidt,
  • Axel Fischer,
  • Lovely Mae F. Lawas,
  • Rejbana Alam,
  • Endang M. Septiningsih,
  • Julia Bailey-Serres,
  • S. V. Krishna Jagadish,
  • Bruno Huettel,
  • Dirk K. Hincha and
  • Ellen Zuther

31 October 2020

The wide natural variation present in rice is an important source of genes to facilitate stress tolerance breeding. However, identification of candidate genes from RNA-Seq studies is hampered by the lack of high-quality genome assemblies for the most...

  • Article
  • Open Access
1 Citations
2,278 Views
19 Pages

Diversity Analysis of Intestinal Bifidobacteria in the Hohhot Population

  • Shuying Yang,
  • Su Wu,
  • Feiyan Zhao,
  • Zhixin Zhao,
  • Xin Shen,
  • Xia Yu,
  • Meng Zhang,
  • Fang Wen,
  • Zhihong Sun and
  • Bilige Menghe

(1) Background: Bifidobacterium plays a pivotal role within the gut microbiota, significantly affecting host health through its abundance and composition in the intestine. Factors such as age, gender, and living environment exert considerable influen...

  • Article
  • Open Access
9 Citations
3,226 Views
19 Pages

27 December 2022

Gray mold (Botrytis elliptica) causes a deleterious fungal disease that decreases the ornamental value and yield of lilies. Lilium oriental hybrid ‘Sorbonne’ is a variety that is resistant to gray mold. Understanding the mechanism of resi...

  • Article
  • Open Access
18 Citations
3,755 Views
10 Pages

20 December 2019

Functional (meta) genomics allows the high-throughput identification of functional genes in a premise-free way. However, it is still difficult to perform Sanger sequencing for high GC DNA templates, which hinders the functional genomic exploration of...

  • Article
  • Open Access
5 Citations
4,085 Views
20 Pages

16 September 2021

While recent sequencing technologies (third generation sequencing) can successfully sequence all copies of nuclear ribosomal DNA (rDNA) markers present within a genome and offer insights into the intragenomic variation of these markers, high intragen...

  • Article
  • Open Access
108 Citations
27,420 Views
11 Pages

A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing

  • Sarah B. Kingan,
  • Haynes Heaton,
  • Juliana Cudini,
  • Christine C. Lambert,
  • Primo Baybayan,
  • Brendan D. Galvin,
  • Richard Durbin,
  • Jonas Korlach and
  • Mara K. N. Lawniczak

18 January 2019

A high-quality reference genome is a fundamental resource for functional genetics, comparative genomics, and population genomics, and is increasingly important for conservation biology. PacBio Single Molecule, Real-Time (SMRT) sequencing generates lo...

  • Article
  • Open Access
33 Citations
7,582 Views
14 Pages

The First Highly Contiguous Genome Assembly of Pikeperch (Sander lucioperca), an Emerging Aquaculture Species in Europe

  • Julien Alban Nguinkal,
  • Ronald Marco Brunner,
  • Marieke Verleih,
  • Alexander Rebl,
  • Lidia de los Ríos-Pérez,
  • Nadine Schäfer,
  • Frieder Hadlich,
  • Marcus Stüeken,
  • Dörte Wittenburg and
  • Tom Goldammer

13 September 2019

The pikeperch (Sander lucioperca) is a fresh and brackish water Percid fish natively inhabiting the northern hemisphere. This species is emerging as a promising candidate for intensive aquaculture production in Europe. Specific traits like cannibalis...

  • Article
  • Open Access
5 Citations
3,756 Views
10 Pages

First Draft Genome Assembly of Root-Lesion Nematode Pratylenchus scribneri Generated Using Long-Read Sequencing

  • Deepika Arora,
  • Alvaro G. Hernandez,
  • Kimberly K. O. Walden,
  • Christopher J. Fields and
  • Guiping Yan

Root-lesion nematodes (genus Pratylenchus) belong to a diverse group of plant-parasitic nematodes (PPN) with a worldwide distribution. Despite being an economically important PPN group of more than 100 species, genome information related to Pratylenc...

  • Article
  • Open Access
7 Citations
9,748 Views
21 Pages

The Isolation and Full-Length Transcriptome Sequencing of a Novel Nidovirus and Response of Its Infection in Japanese Flounder (Paralichthys olivaceus)

  • Chunguang Gong,
  • Yitong Zhang,
  • Guixing Wang,
  • Yufeng Liu,
  • Zhongwei He,
  • Yuqin Ren,
  • Wei Cao,
  • Haitao Zhao,
  • Yuhao Xu and
  • Jilun Hou
  • + 1 author

2 June 2022

A novel nidovirus, CSBV Bces-Po19, was isolated from the marine fish, Japanese flounder (Paralichthys olivaceus). The viral genome was 26,597 nucleotides long and shared 98.62% nucleotide identity with CSBV WHQSR4345. PacBio Sequel and Illumina seque...

  • Article
  • Open Access
34 Citations
6,482 Views
16 Pages

Whole Genome Sequencing of the Giant Grouper (Epinephelus lanceolatus) and High-Throughput Screening of Putative Antimicrobial Peptide Genes

  • Dengdong Wang,
  • Xiyang Chen,
  • Xinhui Zhang,
  • Jia Li,
  • Yunhai Yi,
  • Chao Bian,
  • Qiong Shi,
  • Haoran Lin,
  • Shuisheng Li and
  • Xinxin You
  • + 1 author

28 August 2019

Giant groupers, the largest grouper type in the world, are of economic importance in marine aquaculture for their rapid growth. At the same time, bacterial and viral diseases have become the main threats to the grouper industry. Here, we report a hig...

  • Article
  • Open Access
9 Citations
3,865 Views
16 Pages

17 December 2019

The limitations of RNA sequencing make it difficult to accurately predict alternative splicing (AS) and alternative polyadenylation (APA) events and long non-coding RNAs (lncRNAs), all of which reveal transcriptomic diversity and the complexity of ge...

  • Article
  • Open Access
5 Citations
3,094 Views
17 Pages

10 November 2023

This study is the first to report the characterization of Carex pumila genomic information. Assembly of the genome generated a draft of C. pumila based on PacBio Sequel II and Illumina paired-end sequencing, which was assembled from 2941 contigs with...

  • Communication
  • Open Access
19 Citations
3,353 Views
11 Pages

Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy

  • Vittoria Mattioni Marchetti,
  • Ibrahim Bitar,
  • Mario Sarti,
  • Elena Fogato,
  • Erika Scaltriti,
  • Chiara Bracchi,
  • Jaroslav Hrabak,
  • Stefano Pongolini and
  • Roberta Migliavacca

Background: the co-production of carbapenemases and mcr-genes represents a worrisome event in the treatment of Enterobacteriaceae infections. The aim of the study was to characterize the genomic features of two clinical Enterobacter cloacae complex (...

  • Article
  • Open Access
3 Citations
1,787 Views
18 Pages

Genomic Analysis of Penicillium griseofulvum CF3 Reveals Potential for Plant Growth Promotion and Disease Resistance

  • Jianfei Yang,
  • Wenshuai Zang,
  • Jie Chen,
  • Dongying Lu,
  • Ruotong Li,
  • Ciyun Li,
  • Yinhua Chen,
  • Qin Liu and
  • Xiaolei Niu

17 February 2025

Penicillium griseofulvum CF3 is a fungus isolated from healthy strawberry soil, with the potential to promote the growth of plants and enhance their resistance to diseases. However, the genome sequence of P. griseofulvum CF3 remains unclear. Therefor...

  • Article
  • Open Access
3 Citations
2,163 Views
17 Pages

Genome Sequencing and Metabolic Potential Analysis of Irpex lacteus

  • Yue Wang,
  • Yingce Duan,
  • Menghan Zhang,
  • Chaoqin Liang,
  • Wenli Li,
  • Chengwei Liu and
  • Ying Ye

7 December 2024

Irpex lacteus is an edible and medicinal macrofungus with significant biological activity and broad pharmaceutical prospects that has received increasing attention in recent years. Although it is an important resource for macrofungi, knowledge of it...

  • Article
  • Open Access
2 Citations
1,877 Views
20 Pages

4 March 2025

Agaricus bisporus, a globally cultivated edible fungus, faces significant challenges from fungal diseases like cobweb disease caused by Cladobotryum mycophilum, which severely impacts yield. This study aimed to explore the genetic basis of disea...

  • Article
  • Open Access
1 Citations
957 Views
16 Pages

14 April 2025

This study aims to elucidate the characteristics of the mitogenome of Lysmata lipkei and investigate its phylogenetic relationships. Using both the Illumina NovaSeq 6000 (Illumina, Inc., San Diego, CA, USA) and PacBio Sequel II (Pacific Biosciences o...

  • Article
  • Open Access
4 Citations
3,225 Views
18 Pages

Genome Characteristics of the Endophytic Fungus Talaromyces sp. DC2 Isolated from Catharanthus roseus (L.) G. Don

  • Nguyen Duc Quan,
  • Ngoc-Lan Nguyen,
  • Tran Thi Huong Giang,
  • Nguyen Thi Thanh Ngan,
  • Nguyen Thanh Hien,
  • Nguyen Van Tung,
  • Nguyen Hoang Thanh Trang,
  • Nguyen Thi Kim Lien and
  • Huy Hoang Nguyen

15 May 2024

Talaromyces sp. DC2 is an endophytic fungus that was isolated from the stem of Catharanthus roseus (L.) G. Don in Hanoi, Vietnam and is capable of producing vinca alkaloids. This study utilizes the PacBio Sequel technology to completely sequence the...

  • Article
  • Open Access
7 Citations
5,448 Views
25 Pages

5 December 2021

Coral–zooxanthellae holobionts are one of the most productive ecosystems in the ocean. With global warming and ocean acidification, coral ecosystems are facing unprecedented challenges. To save the coral ecosystems, we need to understand the sy...

  • Article
  • Open Access

19 March 2026

Nigrospora musae ST1 is a newly identified pathogen responsible for leaf spot disease in Idesia polycarpa. In order to further advance our understanding of this strain and improve management strategies for the leaf spot disease, the PacBio Sequel II...

  • Article
  • Open Access
1 Citations
2,016 Views
18 Pages

Comparative Genomic Analysis of Two Monokaryons of Auricularia heimuer Hei29

  • Fengli Wang,
  • Chuang Han,
  • Jiechi Zhang,
  • Piqi Zhang,
  • Xiaojia Zhang,
  • Xin Yue,
  • Yanshu Zhao and
  • Xiaodong Dai

6 February 2025

Auricularia heimuer is a valuable traditional Chinese fungus used as food and medicine. Hei29 is a strain derived from wild A. heimuer through systematic domestication and selection. It has been the primary A. heimuer variety in Northeast China for 3...

  • Article
  • Open Access
1 Citations
1,352 Views
25 Pages

3 July 2025

Background: Pinus massoniana is a significant lipid-producing tree species in China and a susceptible host for both the pine wood nematode and its insect vector, Monochamus alternatus. The basic helix–loop–helix (bHLH) family of transcrip...

  • Article
  • Open Access
5 Citations
2,204 Views
12 Pages

22 May 2024

Neocaridina denticulata sinensis has emerged as a promising model organism for basic studies in Decapod. However, the current transcriptome information on this species is based on next-generation sequencing technologies, which are limited by a short...

  • Article
  • Open Access
2 Citations
1,423 Views
12 Pages

11 September 2023

(1) Background: Leptocybe invasa (Hymenoptera: Eulophidae) is a global invasive pest that seriously damages eucalyptus plants and has caused serious harm to forestry production in many countries. Two genotypically distinct lineages of L. invasa have...

  • Article
  • Open Access
4 Citations
2,850 Views
19 Pages

Application of Dual Metabarcoding Platforms for the Meso- and Macrozooplankton Taxa in the Ross Sea

  • Ji-Hyun Lee,
  • Hyoung Sul La,
  • Jeong-Hoon Kim,
  • Wuju Son,
  • Hyun Park,
  • Young-Mog Kim and
  • Hyun-Woo Kim

21 May 2022

Meso- and macrozooplankton play crucial roles in the trophic web and the biological carbon pump in the ocean by transferring energy from lower to higher trophic levels and vertically exporting carbon from the surface to the deep ocean and seabed. In...

  • Article
  • Open Access
824 Views
13 Pages

Protein-Calorie Malnutrition Is Associated with Altered Colonic Mucosal Microbiota in Inflammatory Bowel Disease

  • Hyo-Joon Yang,
  • Melissa Corson,
  • Ezinne Aja,
  • Ellen Spartz,
  • Berkeley N. Limketkai and
  • Jonathan P. Jacobs

1 December 2025

Background/Objectives: Protein-calorie malnutrition (PCM) is common among patients with inflammatory bowel disease (IBD). However, the relationship between PCM and the gut microbiota in patients with IBD remains unclear. This study aimed to investiga...

  • Article
  • Open Access
3,070 Views
19 Pages

A Native Insect on a Non-Native Plant: The Phylogeography of the Leafminer Phyllonorycter populifoliella (Lepidoptera: Gracillariidae) Attacking the North American Balsam Poplar in North Asia

  • Natalia I. Kirichenko,
  • Maria A. Ryazanova,
  • Evgeny N. Akulov,
  • Svetlana V. Baryshnikova,
  • Anton A. Efremenko,
  • Konstantin V. Krutovsky,
  • Victor Ya. Kuzevanov,
  • Andrei V. Selikhovkin,
  • Pathour R. Shashank and
  • Dmitrii L. Musolin
  • + 3 authors

21 January 2025

The trans-Palearctic moth Phyllonorycter populifoliella (Lepidoptera: Gracillariidae) is a major pest of the North American Populus balsamifera and its hybrids widely planted as ornamentals in North Asia (i.e., the Asian part of Russia). We DNA barco...

  • Article
  • Open Access
5 Citations
2,034 Views
22 Pages

20 May 2025

Auricularia mushrooms, common bulk edible fungi, have considerable culinary and medicinal value. The Qinling region, represented by Zhashui County, is the main production area of Auricularia mushrooms in China. In this study, two wild Auricularia str...

  • Article
  • Open Access
8 Citations
2,575 Views
27 Pages

Whole-Genome Sequence Analysis of Flammulina filiformis and Functional Validation of Gad, a Key Gene for γ-Aminobutyric Acid Synthesis

  • Wenyun Li,
  • Junjun Shang,
  • Dapeng Bao,
  • Jianing Wan,
  • Chenli Zhou,
  • Zhan Feng,
  • Hewen Li,
  • Youran Shao and
  • Yingying Wu

12 December 2024

Flammulina filiformis is one of the widely produced edible fungi worldwide. It is rich in γ-aminobutyric acid (GABA), a non-protein amino acid with important physiological functions in humans. To investigate the functions of key genes in the GA...

  • Article
  • Open Access
1 Citations
920 Views
23 Pages

The actinobacterial strain Kitasatospora griseola JNUCC 62 was isolated from volcanic wetland soil at Mulyeongari Oreum, Jeju Island, and taxonomically identified through 16S rRNA gene and whole-genome analyses. The complete genome, assembled from Pa...

  • Article
  • Open Access
15 Citations
3,814 Views
12 Pages

Characterization and Phylogenetic Analyses of the Complete Mitochondrial Genome of Sugarcane (Saccharum spp. Hybrids) Line A1

  • Dinggang Zhou,
  • Ying Liu,
  • Jingzuo Yao,
  • Ze Yin,
  • Xinwen Wang,
  • Liping Xu,
  • Youxiong Que,
  • Ping Mo and
  • Xiaolan Liu

25 April 2022

Modern sugarcane cultivars are highly polyploid with complex nuclear genomic genetic background, while their mitochondrion (mt) genomes are much simpler, smaller and more manageable and could provide useful phylogenetic information. In this study, th...

  • Article
  • Open Access
6 Citations
3,181 Views
13 Pages

27 January 2023

Ficus simplicissima Lour. is an Asian species of fig tree in the family Moraceae. The chloroplast (cp) genome of F. simplicissima m3 was sequenced using the Pacbio sequel platform. The F. simplicissima cpDNA has a size of 160,321 bp in length, of whi...

  • Article
  • Open Access
9 Citations
3,545 Views
11 Pages

2 January 2023

In the last decade, the phylogenetic relationships within the genus Triplophysa have become controversial, due to a lack of molecular data. The mitochondrial genome plays a vital role in the reconstruction of phylogenetic relationships and in reveali...