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Whole Genome Sequencing of the Giant Grouper (Epinephelus lanceolatus) and High-Throughput Screening of Putative Antimicrobial Peptide Genes

by Dengdong Wang 1,2,†, Xiyang Chen 3,4,†, Xinhui Zhang 4, Jia Li 4, Yunhai Yi 3,4, Chao Bian 4, Qiong Shi 3,4,5, Haoran Lin 1,2, Shuisheng Li 1,2,*, Yong Zhang 1,2,* and Xinxin You 3,4,*
1
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China
2
Zhanjiang Bay Laboratory, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
3
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
4
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China
5
Laboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2019, 17(9), 503; https://doi.org/10.3390/md17090503
Received: 30 July 2019 / Revised: 20 August 2019 / Accepted: 26 August 2019 / Published: 28 August 2019
(This article belongs to the Special Issue Genetics of Marine Organisms Associated with Human Health)
Giant groupers, the largest grouper type in the world, are of economic importance in marine aquaculture for their rapid growth. At the same time, bacterial and viral diseases have become the main threats to the grouper industry. Here, we report a high-quality genome of a giant grouper sequenced by an Illumina HiSeq X-Ten and PacBio Bioscience Sequel platform. A total of 254 putative antimicrobial peptide (AMP) genes were identified, which can be divided into 34 classes according to the annotation of the Antimicrobial Peptides Database (APD3). Their locations in pseudochromosomes were also determined. Thrombin-, lectin-, and scolopendin-derived putative AMPs were the three largest parts. In addition, expressions of putative AMPs were measured by our transcriptome data. Two putative AMP genes (gapdh1 and gapdh2) were involved in glycolysis, which had extremely high expression levels in giant grouper muscle. As it has been reported that AMPs inhibit the growth of a broad spectrum of microbes and participate in regulating innate and adaptive immune responses, genome sequencing of this study provides a comprehensive cataloging of putative AMPs of groupers, supporting antimicrobial research and aquaculture therapy. These genomic resources will be beneficial to further molecular breeding of this economically important fish. View Full-Text
Keywords: giant grouper; Epinephelus lanceolatus; genome sequencing; antimicrobial peptide; growth giant grouper; Epinephelus lanceolatus; genome sequencing; antimicrobial peptide; growth
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MDPI and ACS Style

Wang, D.; Chen, X.; Zhang, X.; Li, J.; Yi, Y.; Bian, C.; Shi, Q.; Lin, H.; Li, S.; Zhang, Y.; You, X. Whole Genome Sequencing of the Giant Grouper (Epinephelus lanceolatus) and High-Throughput Screening of Putative Antimicrobial Peptide Genes. Mar. Drugs 2019, 17, 503.

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