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258 Results Found

  • Article
  • Open Access
12 Citations
3,213 Views
15 Pages

3 September 2021

Seashore paspalum is a major warm-season turfgrass requiring frequent mowing. The use of dwarf cultivars with slow growth is a promising method to decrease mowing frequency. The present study was conducted to provide an in-depth understanding of the...

  • Article
  • Open Access
22 Citations
6,385 Views
22 Pages

iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachment

  • Fátima Milhano Santos,
  • Leonor Mesquita Gaspar,
  • Sergio Ciordia,
  • Ana Sílvia Rocha,
  • João Paulo Castro e Sousa,
  • Alberto Paradela,
  • Luís António Passarinha and
  • Cândida Teixeira Tomaz

Rhegmatogenous retinal detachment (RRD) is a potentially blinding condition characterized by a physical separation between neurosensory retina and retinal pigment epithelium. Quantitative proteomics can help to understand the changes that occur at th...

  • Article
  • Open Access
4 Citations
3,900 Views
19 Pages

ITRAQ Proteomic Analysis of Yellow and Black Skin in Jinbian Carp (Cyprinus carpio)

  • Xiangchen Ye,
  • Lingling Zhou,
  • Jingyi Jia,
  • Lingjing Wei,
  • Yanhong Wen,
  • Xueyu Yan,
  • Jie Huang,
  • Baojiang Gan,
  • Kang Liu and
  • Guangfu Hu
  • + 1 author

30 September 2020

Colors are important phenotypic traits for fitness under natural conditions in vertebrates. Previous studies have reported several functional genes and genetic variations of pigmentation, but the formation mechanisms of various skin coloration remain...

  • Article
  • Open Access
1,753 Views
13 Pages

Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology

  • Xin Ma,
  • Meng Wang,
  • Jinglei Wang,
  • Qian Zhang,
  • Sisi Pu,
  • Rui Wang,
  • Sijiu Yu,
  • Libin Wang and
  • Yangyang Pan

23 June 2023

The aim of this study was to investigate protein regulation at different time points during the in vitro maturation of yak oocytes. Yak oocytes at GV, MI, and MII stages were collected during in vitro maturation, and differential proteomics sequencin...

  • Article
  • Open Access
5 Citations
3,009 Views
18 Pages

14 December 2022

The “iron hypothesis” of atherosclerosis has long been controversial. Several studies have shown that dietary iron restriction or low-iron diets can effectively alleviate atherosclerosis in rabbits and mice. However, the underlying molecu...

  • Article
  • Open Access
28 Citations
6,063 Views
21 Pages

iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A

  • Yan Wang,
  • Xiaoli Peng,
  • Zhuojun Yang,
  • Weiwei Zhao,
  • Wentao Xu,
  • Junran Hao,
  • Weihong Wu,
  • Xiao Li Shen,
  • Yunbo Luo and
  • Kunlun Huang

18 May 2017

Ochratoxin A (OTA) is one of the most common and dangerous mycotoxins in the world. Previous work indicated that OTA could elicit spontaneous HR-like lesions formation Arabidopsis thaliana, reactive oxygen species (ROS) play an important role in OTA...

  • Article
  • Open Access
18 Citations
4,837 Views
17 Pages

Study on Differential Protein Expression in Natural Selenium-Enriched and Non-Selenium-Enriched Rice Based on iTRAQ Quantitative Proteomics

  • Rui Zeng,
  • Muhammad Umer Farooq,
  • Li Wang,
  • Yang Su,
  • Tengda Zheng,
  • Xiaoying Ye,
  • Xiaomei Jia and
  • Jianqing Zhu

30 March 2019

This work was designated to scrutinize the protein differential expression in natural selenium-enriched and non-selenium-enriched rice using the Isobaric-tags for relative and absolute quantification (iTRAQ) proteomics approach. The extracted protein...

  • Article
  • Open Access
3 Citations
4,060 Views
12 Pages

Proteomic Analysis of Beef Tenderloin and Flank Assessed Using an Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)

  • Zhaomin Lei,
  • Jianping Wu,
  • Deyin Zhang,
  • Ting Liu,
  • Shengguo Zhao,
  • Jianfu Wang and
  • Xiaoxue Zhang

16 January 2020

Herein, we performed a proteomic analysis of tenderloin and flank steaks from Simmental cattle using the isobaric tags for a relative and absolute quantification (iTRAQ) approach. We identified 17 amino acids in both steaks, and Gly, Cys, Ile, Lys, a...

  • Article
  • Open Access
5 Citations
2,924 Views
15 Pages

7 February 2022

Extended lactation is a common phenomenon in lactating yaks under grazing and natural reproduction conditions. To elucidate differences in milk protein compositions and mammary gland functions between yaks of standard lactation (TL yaks) and prolonge...

  • Article
  • Open Access
2,665 Views
13 Pages

18 April 2022

Vacuum-packed sauce lamb tripe was subjected to secondary pasteurization by high-pressure processing (HPP) and heat treatment (HT), and iTRAQ technology was applied to investigate the differentially expressed proteins (DEPs). The analysis revealed 48...

  • Article
  • Open Access
14 Citations
3,333 Views
16 Pages

The Effect of Dietary Lycopene Supplementation on Drip Loss during Storage of Lamb Meat by iTRAQ Analysis

  • Bo Wang,
  • Chen-chen Xu,
  • Ce Liu,
  • Yang-hua Qu,
  • Hao Zhang and
  • Hai-ling Luo

29 January 2021

This study was designed to investigate the impact of dietary lycopene (antioxidant extracted from tomato) supplementation on postmortem antioxidant capacity, drip loss and protein expression profiles of lamb meat during storage. Thirty male Hu lambs...

  • Article
  • Open Access
40 Citations
6,037 Views
20 Pages

Comparative Proteomic Analysis by iTRAQ Reveals that Plastid Pigment Metabolism Contributes to Leaf Color Changes in Tobacco (Nicotiana tabacum) during Curing

  • Shengjiang Wu,
  • Yushuang Guo,
  • Muhammad Faheem Adil,
  • Shafaque Sehar,
  • Bin Cai,
  • Zhangmin Xiang,
  • Yonggao Tu,
  • Degang Zhao and
  • Imran Haider Shamsi

Tobacco (Nicotiana tabacum), is a world’s major non-food agricultural crop widely cultivated for its economic value. Among several color change associated biological processes, plastid pigment metabolism is of trivial importance in postharvest...

  • Article
  • Open Access
26 Citations
4,315 Views
21 Pages

Identification of Differentially Expressed Proteins in Sugarcane in Response to Infection by Xanthomonas albilineans Using iTRAQ Quantitative Proteomics

  • Jian-Yu Meng,
  • Mbuya Sylvain Ntambo,
  • Philippe C. Rott,
  • Hua-Ying Fu,
  • Mei-Ting Huang,
  • Hui-Li Zhang and
  • San-Ji Gao

Sugarcane can suffer severe yield losses when affected by leaf scald, a disease caused by Xanthomonas albilineans. This bacterial pathogen colonizes the vascular system of sugarcane, which can result in reduced plant growth and plant death. In order...

  • Article
  • Open Access
15 Citations
7,125 Views
12 Pages

Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ

  • Shu-Wu Xie,
  • Guo-Ting Li,
  • Li-Juan Qu,
  • Yang Cao,
  • Qian Wang,
  • Jie-Yun Zhou,
  • Rui-Hua Zhong,
  • Xiang-Jie Guo and
  • Yan Zhu

Background: Spermatozoa become mature and acquire fertilizing capacity during their passage through the epididymal lumen. In this study, we identified new epididymal luminal fluid proteins involved in sperm maturation in infertile rats by dutasteride...

  • Article
  • Open Access
2 Citations
3,397 Views
17 Pages

Analysis of Photosynthetic Differences of Rice Germplasm in Southeast Asia Based on Leaf-Tissue Structure, Physiology, and iTRAQ

  • Xiaoli Zhang,
  • Maoyan Tang,
  • Hui Wang,
  • Wei Tao,
  • Qiang Wang,
  • Lei Chen,
  • Guoqing Gao,
  • Ronghua Lv and
  • Tianfeng Liang

16 December 2022

Photosynthesis is responsible for 90–95% of organic matter in crop yield. The light energy utilization rate of high-yielding rice varieties is 1.0–1.5%, but the ideal value is about 3–5%. Yield can be further improved by improving p...

  • Article
  • Open Access
10 Citations
3,044 Views
13 Pages

5 June 2022

Dwarf bunt and common bunt diseases of wheat are caused by Tilletia controversa Kühn and Tilletia foetida Kühn, respectively, and losses caused by these diseases can reach 70–80% in favourable conditions. T. controversa and T. foetida...

  • Article
  • Open Access
25 Citations
6,614 Views
13 Pages

25 October 2016

As the only known organ that can completely regenerate in mammals, deer antler is of real significance in the field of regenerative medicine. Recent studies have shown that the regenerative capacity of the antlers comes from the pedicle periosteum an...

  • Article
  • Open Access
13 Citations
4,341 Views
19 Pages

10 March 2020

Phalaenopsis amabilis, one of the most important plants in the international flower market due to its graceful shape and colorful flowers, is an orchid that undergoes vernalization and requires low-temperature treatment for flowering. There have been...

  • Article
  • Open Access
17 Citations
4,730 Views
19 Pages

Proteomic Changes during MCMV Infection Revealed by iTRAQ Quantitative Proteomic Analysis in Maize

  • Mingqing Dang,
  • Qi Cheng,
  • Ya Hu,
  • Jianxiang Wu,
  • Xueping Zhou and
  • Yajuan Qian

Maize chlorotic mottle virus (MCMV) has been occurring frequently worldwide and causes severe yield losses in maize (Zea mays). To better investigate the destructive effects of MCMV infection on maize plants, isobaric tagging for relative and absolut...

  • Article
  • Open Access
23 Citations
4,050 Views
17 Pages

26 November 2019

Drought stress, especially during the seedling stage, seriously limits the growth of maize and reduces production in the northeast of China. To investigate the molecular mechanisms of drought response in maize seedlings, proteome changes were analyze...

  • Article
  • Open Access
52 Citations
6,841 Views
13 Pages

The sea cucumber Apostichopus japonicus is exploited as a commercial species owing to their high nutritive and medicinal value. Recent high summer temperatures have caused high mortality rates in A. japonicus. In this study, we applied the isobaric t...

  • Article
  • Open Access
22 Citations
7,717 Views
16 Pages

28 September 2016

Fluorosis induced by exposure to high level fluoride is quite widespread in the world. The manifestations of fluorosis include dental mottling, bone damage, and impaired malfunction of soft tissues. However, the molecular mechanism of fluorosis has n...

  • Article
  • Open Access
13 Citations
4,221 Views
17 Pages

iTRAQ-Based Proteomic Analysis of Ogura-CMS Cabbage and Its Maintainer Line

  • Fengqing Han,
  • Xiaoli Zhang,
  • Limei Yang,
  • Mu Zhuang,
  • Yangyong Zhang,
  • Zhansheng Li,
  • Zhiyuan Fang and
  • Honghao Lv

15 October 2018

Ogura cytoplasmic male sterility (CMS) contributes considerably to hybrid seed production in Brassica crops. To detect the key protein species and pathways involved in Ogura-CMS, we analysed the proteome of the cabbage Ogura-CMS line CMS01-20 and its...

  • Article
  • Open Access
7 Citations
3,097 Views
19 Pages

7 November 2021

Thaxtomin A (TA) is a phytotoxin secreted by Streptomyces scabies that causes common scab in potatoes. However, the mechanism of potato proteomic changes in response to TA is barely known. In this study, the proteomic changes in potato leaves treated...

  • Article
  • Open Access
21 Citations
4,633 Views
14 Pages

iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties

  • Shumei Li,
  • Xueqiang Su,
  • Qing Jin,
  • Guohui Li,
  • Yanming Sun,
  • Muhammad Abdullah,
  • Yongping Cai and
  • Yi Lin

Most pears in Anhui Province are a kind of self-incompatible fruit whose quality is strongly influenced by the male pollen. The proteomic variation of Dangshan Su pollinated by different varieties was analysed using the isobaric tag for relative and...

  • Article
  • Open Access
12 Citations
4,594 Views
13 Pages

25 October 2017

The abalone Haliotis discus hannai is an important aquaculture species that is grown for human consumption. However, little is known of the genetic mechanisms governing muscle growth in this species, particularly with respect to proteomics. The isoba...

  • Article
  • Open Access
25 Citations
5,134 Views
19 Pages

iTRAQ-Based Quantitative Analysis of Responsive Proteins Under PEG-Induced Drought Stress in Wheat Leaves

  • Yajing Wang,
  • Xinying Zhang,
  • Guirong Huang,
  • Fu Feng,
  • Xiaoying Liu,
  • Rui Guo,
  • Fengxue Gu,
  • Xiuli Zhong and
  • Xurong Mei

Drought is an important abiotic stress that seriously restricts crop productivity. An understanding of drought tolerance mechanisms offers guidance for cultivar improvement. In order to understand how a well-known wheat genotype Jinmai 47 responds to...

  • Article
  • Open Access
10 Citations
3,749 Views
12 Pages

Identification of Important Proteins and Pathways Affecting Feed Efficiency in DLY Pigs by iTRAQ-Based Proteomic Analysis

  • Jie Wu,
  • Xingwang Wang,
  • Rongrong Ding,
  • Jianping Quan,
  • Yong Ye,
  • Ting Gu,
  • Zheng Xu,
  • Enqin Zheng,
  • Gengyuan Cai and
  • Jie Yang
  • + 2 authors

22 January 2020

Feed efficiency is an economically important trait controlled by multiple genes in pigs. The small intestine is the main organ of digestion and nutrient absorption. To explore the biological processes by which small intestine proteomics affects feed...

  • Article
  • Open Access
25 Citations
7,390 Views
17 Pages

7 December 2016

Rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) is a complicated and serious type of rhegmatogenous retinal detachment (RRD). In this study, we identified differentially expressed proteins in the vitreous humors of RRDC...

  • Article
  • Open Access
15 Citations
4,478 Views
20 Pages

iTRAQ-Based Analysis of Proteins Co-Regulated by Brassinosteroids and Gibberellins in Rice Embryos during Seed Germination

  • Qian-Feng Li,
  • Jin-Dong Wang,
  • Min Xiong,
  • Ke Wei,
  • Peng Zhou,
  • Li-Chun Huang,
  • Chang-Quan Zhang,
  • Xiao-Lei Fan and
  • Qiao-Quan Liu

4 November 2018

Seed germination, a pivotal process in higher plants, is precisely regulated by various external and internal stimuli, including brassinosteroid (BR) and gibberellin (GA) phytohormones. The molecular mechanisms of crosstalk between BRs and GAs in reg...

  • Article
  • Open Access
6 Citations
2,464 Views
16 Pages

21 September 2022

Brassica napus inap cytoplasmic male sterility (CMS) is a novel sterile line with potential application in rapeseed hybrid breeding. Sterile cytoplasm was obtained from Isatis indigotica through somatic fusion and then recurrent backcrossing with B....

  • Article
  • Open Access
37 Citations
4,794 Views
17 Pages

7 December 2018

To uncover mechanism of highly weakened carbon metabolism in chlorotic tea (Camellia sinensis) plants, iTRAQ (isobaric tags for relative and absolute quantification)-based proteomic analyses were employed to study the differences in protein expressio...

  • Article
  • Open Access
8 Citations
6,708 Views
21 Pages

4 December 2019

The silkworm is an oligophagous insect for which mulberry leaves are the sole diet. The nutrients needed for vital activities of the egg, pupal, and adult stages, and the proteins formed in the cocoon, are all derived from the larval stages. The silk...

  • Article
  • Open Access
13 Citations
4,762 Views
14 Pages

Proteomics Analysis to Identify Proteins and Pathways Associated with the Novel Lesion Mimic Mutant E40 in Rice Using iTRAQ-Based Strategy

  • Xiang-Bo Yang,
  • Wei-Long Meng,
  • Meng-Jie Zhao,
  • An-Xing Zhang,
  • Wei Liu,
  • Zhao-Shi Xu,
  • Yun-Peng Wang and
  • Jian Ma

A novel rice lesion mimic mutant (LMM) was isolated from the mutant population of Japonica rice cultivar Hitomebore generated by ethyl methane sulfonate (EMS) treatment. Compared with the wild-type (WT), the mutant, tentatively designated E40, develo...

  • Article
  • Open Access
908 Views
15 Pages

Exploring the Proteomic Landscape of Cochlear Implant Trauma: An iTRAQ-Based Quantitative Analysis Utilizing an Ex Vivo Model

  • Jake Langlie,
  • Rahul Mittal,
  • David H. Elisha,
  • Jaimee Cooper,
  • Hannah Marwede,
  • Julian Purrinos,
  • Maria-Pia Tuset,
  • Keelin McKenna,
  • Max Zalta and
  • Adrien A. Eshraghi
  • + 1 author

18 July 2025

Background: Cochlear implantation is widely used to provide auditory rehabilitation to individuals with severe-to-profound sensorineural hearing loss. However, electrode insertion during cochlear implantation leads to inner ear trauma, damage to sens...

  • Article
  • Open Access
19 Citations
5,603 Views
17 Pages

Cucumber green mottle mosaic virus (CGMMV) is an important viral pathogen on cucurbit plants worldwide, which can cause severe fruit decay symptoms on infected watermelon (usually called “watermelon blood flesh”). However, the molecular m...

  • Article
  • Open Access
1 Citations
3,647 Views
18 Pages

iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of Euphorbia kansui L.

  • Xiaoai Fang,
  • Xiangyu Yao,
  • Yue Zhang,
  • Zheni Tian,
  • Meng Wang,
  • Peng Li and
  • Xia Cai

Autophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce...

  • Article
  • Open Access
24 Citations
3,881 Views
16 Pages

iTRAQ-Based Quantitative Proteomic Analysis of Embryogenic and Non-embryogenic Calli Derived from a Maize (Zea mays L.) Inbred Line Y423

  • Beibei Liu,
  • Xiaohui Shan,
  • Ying Wu,
  • Shengzhong Su,
  • Shipeng Li,
  • Hongkui Liu,
  • Junyou Han and
  • Yaping Yuan

12 December 2018

Somatic embryos (SE) have potential to rapidly form a whole plant. Generally, SE is thought to be derived from embryogenic calli (EC). However, in maize, not only embryogenic calli (EC, can generate SE) but also nonembryogenic calli (NEC, can’t...

  • Article
  • Open Access
13 Citations
3,961 Views
19 Pages

9 December 2019

The generation of chrysanthemum (Chrysanthemum × morifolium) flower color is mainly attributed to the accumulation of anthocyanins. Light is one of the key environmental factors that affect the anthocyanin biosynthesis, but the deep molecular m...

  • Article
  • Open Access
40 Citations
7,802 Views
23 Pages

14 September 2017

To uncover the alleviation mechanism of quinclorac stress by salicylic acid (SA), leaf samples of Oryza sativa ssp. Japonica under quinclorac stress with and without SA pre-treatment were analyzed for transcriptional and proteomic profiling to determ...

  • Article
  • Open Access
20 Citations
6,823 Views
19 Pages

27 September 2016

In this study, we conducted the first isobaric tags for relative and absolute quantitation (isobaric tags for relative and absolute quantitation (iTRAQ))-based comparative proteomic analysis of ramie plantlets after 0 (minor drought stress), 24 (mode...

  • Article
  • Open Access
21 Citations
3,782 Views
20 Pages

4 December 2018

Salinity is one of the major abiotic stress factors that limit plant growth and crop yield worldwide. To understand the molecular mechanisms and screen the key proteins in response of sugar beet (Beta vulgaris L.) to salt, in the present study, the p...

  • Article
  • Open Access
29 Citations
4,938 Views
28 Pages

iTRAQ-Based Protein Profiling and Biochemical Analysis of Two Contrasting Rice Genotypes Revealed Their Differential Responses to Salt Stress

  • Sajid Hussain,
  • Chunquan Zhu,
  • Zhigang Bai,
  • Jie Huang,
  • Lianfeng Zhu,
  • Xiaochuang Cao,
  • Satyabrata Nanda,
  • Saddam Hussain,
  • Aamir Riaz and
  • Junhua Zhang
  • + 4 authors

Salt stress is one of the key abiotic stresses causing huge productivity losses in rice. In addition, the differential sensitivity to salinity of different rice genotypes during different growth stages is a major issue in mitigating salt stress in ri...

  • Article
  • Open Access
20 Citations
5,124 Views
13 Pages

18 April 2019

Temperature is one of the most important environmental factors affecting flowering in plants. Adonis amurensis, a perennial herbaceous flower that blooms in early spring in northeast China where the temperature can drop to −15 °C, is an ide...

  • Article
  • Open Access
2 Citations
1,066 Views
18 Pages

RNA-Seq Transcriptomics and iTRAQ Proteomics Analysis Reveal the Dwarfing Mechanism of Blue Fescue (Festuca glauca)

  • Yong Zhang,
  • Peng Han,
  • Ruijie Zhao,
  • Shuhan Yu,
  • Hang Liu,
  • Hong Wu,
  • Jinyang Weng and
  • Hengfeng Zhang

29 November 2024

Blue fescue is a widely used ornamental grass because of its strong ecological adaptability. To maintain the optimal ornamental plant shape, blue fescue requires many nutrients and labor. Using dwarf varieties with slow growth is an effective way to...

  • Article
  • Open Access
9 Citations
2,861 Views
13 Pages

23 October 2022

Coronary artery lesions (CAL) are a major complication of Kawasaki disease (KD). The early prediction of CAL enables the medical personnel to apply adequate medical intervention. We collected the serum samples from the KD patients with CAL (n = 32) a...

  • Article
  • Open Access
30 Citations
6,854 Views
19 Pages

Transcriptome and Proteome-Based Network Analysis Reveals a Model of Gene Activation in Wheat Resistance to Stripe Rust

  • Hong Zhang,
  • Ying Fu,
  • Huan Guo,
  • Lu Zhang,
  • Changyou Wang,
  • Weining Song,
  • Zhaogui Yan,
  • Yajuan Wang and
  • Wanquan Ji

Stripe rust, caused by the pathogen Puccinia striiformis f. sp. tritici (Pst), is an important fungal foliar disease of wheat (Triticum aestivum). To study the mechanism underlying the defense of wheat to Pst, we used the next-generation sequencing a...

  • Article
  • Open Access
50 Citations
7,723 Views
19 Pages

Cytoplasmic male sterility (CMS) where no functional pollen is produced has important roles in wheat breeding. The anther is a unique organ for male gametogenesis and its abnormal development can cause male sterility. However, the mechanisms and regu...

  • Article
  • Open Access
20 Citations
7,248 Views
31 Pages

A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef

  • Rizqah Kamies,
  • Jill M. Farrant,
  • Zerihun Tadele,
  • Gina Cannarozzi and
  • Mohammed Suhail Rafudeen

15 November 2017

The orphan crop, Eragrostis tef, was subjected to controlled drought conditions to observe the physiological parameters and proteins changing in response to dehydration stress. Physiological measurements involving electrolyte leakage, chlorophyll flu...

  • Article
  • Open Access
1 Citations
1,493 Views
19 Pages

28 January 2025

Objective: Haprolid, a novel compound extracted from Myxobacterium, has been proven to possess selective toxicity towards various tumor cells, effectively inhibiting the growth of hepatocellular carcinoma (HCC). However, the underlying molecular mech...

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