Next Article in Journal
Development of a LC-MS/MS Method for the Multi-Mycotoxin Determination in Composite Cereal-Based Samples
Next Article in Special Issue
Differential Gene Expression Analysis of Bovine Macrophages after Exposure to the Penicillium Mycotoxins Citrinin and/or Ochratoxin A
Previous Article in Journal
Transcriptional and Physiological Responses to Nutrient Loading on Toxin Formation and Photosynthesis in Microcystis Aeruginosa FACHB-905
Previous Article in Special Issue
Initial Spore Density Has an Influence on Ochratoxin A Content in Aspergillus ochraceus CGMCC 3.4412 in PDB and Its Interaction with Seeds
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Toxins
Volume 9
Issue 5
10.3390/toxins9050167
toxins-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A

by
Yan Wang
1,2,
Xiaoli Peng
2,3,
Zhuojun Yang
2,
Weiwei Zhao
2,
Wentao Xu
2,4,
Junran Hao
2,
Weihong Wu
2,
Xiao Li Shen
2,5,
Yunbo Luo
2,4 and
Kunlun Huang
2,4,*
1
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China
3
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
4
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
5
School of Public Health, Zunyi Medical University, Zunyi 563000, China
*
Author to whom correspondence should be addressed.
Toxins 2017, 9(5), 167; https://doi.org/10.3390/toxins9050167
Submission received: 5 April 2017 / Revised: 9 May 2017 / Accepted: 15 May 2017 / Published: 18 May 2017
(This article belongs to the Collection Ochratoxins-Collection)
Browse Figures
Versions Notes

Abstract

Ochratoxin A (OTA) is one of the most common and dangerous mycotoxins in the world. Previous work indicated that OTA could elicit spontaneous HR-like lesions formation Arabidopsis thaliana, reactive oxygen species (ROS) play an important role in OTA toxicity, and their major endogenous source is mitochondria. However, there has been no evidence as to whether OTA induces directly PCD in plants until now. In this study, the presence of OTA in Arabidopsis thaliana leaves triggered accelerated respiration, increased production of mitochondrial ROS, the opening of ROS-dependent mitochondrial permeability transition pores and a decrease in mitochondrial membrane potential as well as the release of cytochrome c into the cytosol. There were 42 and 43 significantly differentially expressed proteins identified in response to exposure to OTA for 8 and 24 h, respectively, according to iTRAQ analysis. These proteins were mainly involved in perturbation of the mitochondrial electron transport chain, interfering with ATP synthesis and inducing PCD. Digital gene expression data at transcriptional level was consistent with the cell death induced by OTA being PCD. These results indicated that mitochondrial dysfunction was a prerequisite for OTA-induced PCD and the initiation and execution of PCD via a mitochondrial-mediated pathway.
Keywords: ochratoxin A; Arabidopsis thaliana; programmed cell death; mitochondria; iTRAQ; mitoproteomes ochratoxin A; Arabidopsis thaliana; programmed cell death; mitochondria; iTRAQ; mitoproteomes
Graphical Abstract

Share and Cite

MDPI and ACS Style

Wang, Y.; Peng, X.; Yang, Z.; Zhao, W.; Xu, W.; Hao, J.; Wu, W.; Shen, X.L.; Luo, Y.; Huang, K. iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A. Toxins 2017, 9, 167. https://doi.org/10.3390/toxins9050167

AMA Style

Wang Y, Peng X, Yang Z, Zhao W, Xu W, Hao J, Wu W, Shen XL, Luo Y, Huang K. iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A. Toxins. 2017; 9(5):167. https://doi.org/10.3390/toxins9050167

Chicago/Turabian Style

Wang, Yan, Xiaoli Peng, Zhuojun Yang, Weiwei Zhao, Wentao Xu, Junran Hao, Weihong Wu, Xiao Li Shen, Yunbo Luo, and Kunlun Huang. 2017. "iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A" Toxins 9, no. 5: 167. https://doi.org/10.3390/toxins9050167

APA Style

Wang, Y., Peng, X., Yang, Z., Zhao, W., Xu, W., Hao, J., Wu, W., Shen, X. L., Luo, Y., & Huang, K. (2017). iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A. Toxins, 9(5), 167. https://doi.org/10.3390/toxins9050167

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop