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iTRAQ-Based Analysis of Proteins Co-Regulated by Brassinosteroids and Gibberellins in Rice Embryos during Seed Germination

1
Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, College of Agriculture, Yangzhou University, Yangzhou 225009, China
2
Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(11), 3460; https://doi.org/10.3390/ijms19113460
Received: 10 September 2018 / Revised: 27 October 2018 / Accepted: 1 November 2018 / Published: 4 November 2018
(This article belongs to the Section Molecular Plant Sciences)
Seed germination, a pivotal process in higher plants, is precisely regulated by various external and internal stimuli, including brassinosteroid (BR) and gibberellin (GA) phytohormones. The molecular mechanisms of crosstalk between BRs and GAs in regulating plant growth are well established. However, whether BRs interact with GAs to coordinate seed germination remains unknown, as do their common downstream targets. In the present study, 45 differentially expressed proteins responding to both BR and GA deficiency were identified using isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis during seed germination. The results indicate that crosstalk between BRs and GAs participates in seed germination, at least in part, by modulating the same set of responsive proteins. Moreover, most targets exhibited concordant changes in response to BR and GA deficiency, and gene ontology (GO) indicated that most possess catalytic activity and are involved in various metabolic processes. Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) analysis was used to construct a regulatory network of downstream proteins mediating BR- and GA-regulated seed germination. The mutation of GRP, one representative target, notably suppressed seed germination. Our findings not only provide critical clues for validating BR–GA crosstalk during rice seed germination, but also help to optimise molecular regulatory networks. View Full-Text
Keywords: brassinosteroid; gibberellin; seed germination; iTRAQ; Oryza sativa brassinosteroid; gibberellin; seed germination; iTRAQ; Oryza sativa
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MDPI and ACS Style

Li, Q.-F.; Wang, J.-D.; Xiong, M.; Wei, K.; Zhou, P.; Huang, L.-C.; Zhang, C.-Q.; Fan, X.-L.; Liu, Q.-Q. iTRAQ-Based Analysis of Proteins Co-Regulated by Brassinosteroids and Gibberellins in Rice Embryos during Seed Germination. Int. J. Mol. Sci. 2018, 19, 3460. https://doi.org/10.3390/ijms19113460

AMA Style

Li Q-F, Wang J-D, Xiong M, Wei K, Zhou P, Huang L-C, Zhang C-Q, Fan X-L, Liu Q-Q. iTRAQ-Based Analysis of Proteins Co-Regulated by Brassinosteroids and Gibberellins in Rice Embryos during Seed Germination. International Journal of Molecular Sciences. 2018; 19(11):3460. https://doi.org/10.3390/ijms19113460

Chicago/Turabian Style

Li, Qian-Feng, Jin-Dong Wang, Min Xiong, Ke Wei, Peng Zhou, Li-Chun Huang, Chang-Quan Zhang, Xiao-Lei Fan, and Qiao-Quan Liu. 2018. "iTRAQ-Based Analysis of Proteins Co-Regulated by Brassinosteroids and Gibberellins in Rice Embryos during Seed Germination" International Journal of Molecular Sciences 19, no. 11: 3460. https://doi.org/10.3390/ijms19113460

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