Proteomic Changes during MCMV Infection Revealed by iTRAQ Quantitative Proteomic Analysis in Maize
Abstract
:1. Introduction
2. Results
2.1. Phenotype Shown on MCMV-Infected Maize Plants
2.2. iTRAQ-Based Proteomic Analysis
2.3. Impacts of MCMV Infection on the Maize Global Proteome
2.4. GO and KEGG Analysis of DAPs under MCMV Infection
2.5. Photosynthetic Activity Decreased during MCMV Infection
2.6. Alteration of Ribosomal Protein Abundance during MCMV Infection
2.7. Enrichment of Stress Response-Related Proteins during MCMV Infection
2.8. Differential Regulation of Redox Homeostasis Related Proteins
2.9. Transcriptional Level Analysis for Selected DAPs
2.10. Functional Analyses of Two Selected DAPs through Virus-Induced Gene Silencing
3. Discussion
3.1. Photosynthesis Decreased during MCMV Infection
3.2. Ribosomal Proteins Commonly Affected by MCMV Infection
3.3. Stress Response-Related Proteins Regulated by MCMV Infection
3.4. Redox Regulation in Maize after MCMV Infection
3.5. ZmPDIL-1 Facilitates MCMV Infection
3.6. ZmPrx5 Is a Positive Regulator of MCMV Infection
4. Materials and Methods
4.1. Plant Growth and Virus Inoculation
4.2. Sample Preparation
4.3. iTRAQ Labeling and Strong-Cation-Exchange (SCX) Fractionation
4.4. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
4.5. Proteomics Data Analysis
4.6. Annotation of the Proteins
4.7. Enzyme-Linked Immunosorbent Assay (ELISA)
4.8. Total RNA Extraction and qRT-PCR Assay
4.9. Measurements of Photosynthetic Parameters
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
MCMV | chlorotic mottle virus |
CMV | cucumber mosaic virus |
SCMV | sugarcane mosaic virus |
MDMV | maize dwarf mosaic virus |
Mev | measles Virus |
sgRNAs | subgenomic RNAs |
LOP | lipoxygenase |
ROS | reactive oxygen species |
HSP | heat shock protein |
CAT | catalases |
POD | peroxidases |
SOD | superoxide dismutases |
PDI | protein disulfide isomerases |
Prx | peroxiredoxin protein |
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Dang, M.; Cheng, Q.; Hu, Y.; Wu, J.; Zhou, X.; Qian, Y. Proteomic Changes during MCMV Infection Revealed by iTRAQ Quantitative Proteomic Analysis in Maize. Int. J. Mol. Sci. 2020, 21, 35. https://doi.org/10.3390/ijms21010035
Dang M, Cheng Q, Hu Y, Wu J, Zhou X, Qian Y. Proteomic Changes during MCMV Infection Revealed by iTRAQ Quantitative Proteomic Analysis in Maize. International Journal of Molecular Sciences. 2020; 21(1):35. https://doi.org/10.3390/ijms21010035
Chicago/Turabian StyleDang, Mingqing, Qi Cheng, Ya Hu, Jianxiang Wu, Xueping Zhou, and Yajuan Qian. 2020. "Proteomic Changes during MCMV Infection Revealed by iTRAQ Quantitative Proteomic Analysis in Maize" International Journal of Molecular Sciences 21, no. 1: 35. https://doi.org/10.3390/ijms21010035