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Open AccessArticle

iTRAQ-Based Quantitative Proteomic Analysis of Digestive Juice across the First 48 Hours of the Fifth Instar in Silkworm Larvae

by Pingzhen Xu 1,2,†, Meirong Zhang 1,2,†, Ping Qian 1,2, Jiawei Li 1, Xueyang Wang 1,2 and Yangchun Wu 1,2,*
School of Biotechnology, Jiangsu University of Science and Technology, Sibaidu Rd, Zhenjiang 212018, China
Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Sibaidu Rd, Zhenjiang 212018, China
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(24), 6113;
Received: 4 November 2019 / Revised: 29 November 2019 / Accepted: 3 December 2019 / Published: 4 December 2019
(This article belongs to the Section Molecular Biology)
The silkworm is an oligophagous insect for which mulberry leaves are the sole diet. The nutrients needed for vital activities of the egg, pupal, and adult stages, and the proteins formed in the cocoon, are all derived from the larval stages. The silkworm feeds and grows quickly during the larval stages. In particular, the amount of leaf ingested and digested quickly increases from the ecdysis to the gluttonous stage in the fifth instar period. In this study, we used the iTRAQ proteomic technique to identify and analyze silkworm larval digestive juice proteins during this period. A total of 227 proteins were successfully identified. These were primarily serine protease activity, esterase activity, binding, and serine protease inhibitors, which were mainly involved in the digestion and overcoming the detrimental effects of mulberry leaves. Moreover, 30 genes of the identified proteins were expressed specifically in the midgut. Temporal proteomic analysis of digestive juice revealed developmental dynamic features related to molecular mechanisms of the principal functions of digesting, resisting pathogens, and overruling the inhibitory effects of mulberry leaves protease inhibitors (PIs) with a dynamic strategy, although overruling the inhibitory effects has not yet been confirmed by previous study. These findings will help address the potential functions of digestive juice in silkworm larvae.
Keywords: Bombyx mori; iTRAQ; proteomics; digestive juice Bombyx mori; iTRAQ; proteomics; digestive juice
MDPI and ACS Style

Xu, P.; Zhang, M.; Qian, P.; Li, J.; Wang, X.; Wu, Y. iTRAQ-Based Quantitative Proteomic Analysis of Digestive Juice across the First 48 Hours of the Fifth Instar in Silkworm Larvae. Int. J. Mol. Sci. 2019, 20, 6113.

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