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Applied Biosciences, Volume 5, Issue 3

2026 September - 32 articles

Cover Story: In the circular bioeconomy, converting organic waste into valuable soil amendments such as compost and insect frass requires thorough molecular characterization. This study demonstrates the application of high-resolution magic angle spinning (HRMAS) NMR spectroscopy as a non-destructive platform for direct metabolic profiling of intact matrices without extraction. Analyzing compost and frass from black soldier fly, earthworms, and Tenebrio molitor enabled the first-time detection of glycine betaine via HRMAS NMR in these intact matrices, identifying its role as a key metabolite marker of biotransformation. These betaine signatures remained stable during acquisition, confirming their reliability as biochemical indicators. As betaine is a recognized plant osmoprotectant, these findings highlight both its analytical and functional agronomic value for sustainable agriculture. View this paper
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Articles (32)

  • Article
  • Open Access
405 Views
23 Pages

Genome-Wide Association Analysis Reveals New Loci for Leaf Water Status, Biomass and Plant Architectural Traits in Bread Wheat Under Rainfed Conditions

  • Samira Rustamova,
  • Atabay Jahangirov,
  • Ulduza Gurbanova,
  • Faig Khudayev and ,
  • Jens Léon,
  • Ali Ahmad Naz and
  • Irada Huseynova
  • + 2 authors+ 0 author

Drought during reproductive development and grain filling is a major constraint to bread wheat productivity in rainfed environments. In the present study, we employed genome-wide association analysis in an untapped diversity panel of wheat genotypes...

  • Article
  • Open Access
1 Citations
563 Views
20 Pages

Physicochemical and Microbiological Characterization of Chitosan and Agarose Hydrogel-Based Bioinks Functionalized with Dexamethasone for Wound Healing Applications

  • Camilo Zamora-Ledezma,
  • Dulexy Solano-Orrala,
  • Juan J. Leguineche,
  • Javier García-Molleja and ,
  • Juan P. Fernández-Blázquez,
  • Martin Bergaño-Guzmán,
  • Jesús Romero Pozuelo,
  • María Rosario Baquero,
  • Carmen Lorenzo-Aparicio and
  • Victor H. Guerrero
  • + 7 authors+ 2 authors

This work evaluates agarose (AG)- and chitosan (CS)-based hydrogels incorporating a dexamethasone–cyclodextrin complex (Dex) as potential bioink candidates for wound healing applications. For this purpose, biopolymer hydrogels were formulated a...

(This article belongs to the Special Issue Anatomy and Regenerative Medicine: From Methods to Applications (2nd Edition))
  • Review
  • Open Access
903 Views
37 Pages

Plant Growth-Promoting Rhizobacteria as Sustainable Bioinoculants for Mitigating Climate-Induced Abiotic Stresses

  • Sabia Khan,
  • Md. Abdullah Al Sabbir,
  • Nabela Akter,
  • Ankita Saha and ,
  • Imran Khan,
  • Yuan Xu,
  • Mohammad Golam Mostofa and
  • Md. Motaher Hossain
  • + 3 authors+ 0 author

Extreme temperatures, drought, and salinity are among the most detrimental abiotic stressors limiting global plant productivity, and their frequency has intensified under climate change. These escalating pressures underscore the need for sustainable...

(This article belongs to the Special Issue Feature Reviews for Applied Biosciences)
  • Article
  • Open Access
366 Views
18 Pages

Phenolic Content and Antioxidant Capacity of Tobacco Seed-Derived Products

  • Liliya Stoyanova,
  • Margarita Docheva,
  • Desislava Kirkova and
  • Maria Angelova-Romova

Tobacco seeds (Nicotiana tabacum L.) represent a promising nicotine-free source of bioactive compounds, particularly polyphenols and natural antioxidants. This study evaluated the total phenolic content (TPC) and antioxidant activity of tobacco seeds...

(This article belongs to the Topic Biomass Use and its Health and Environmental Effects)
  • Article
  • Open Access
457 Views
19 Pages

Obesity is a multifactorial metabolic disorder characterized by dysregulated energy homeostasis, impaired lipid metabolism, and altered hypothalamic appetite signaling. This study aimed to evaluate the anti-obesity effects and underlying mechanisms o...

(This article belongs to the Topic Research on Natural Products of Medical Plants)
  • Article
  • Open Access
606 Views
20 Pages

In-Silico Identification of Bee Venom Peptides (Melittin and Tertiapin) as Potential Anti-Angiogenic Agents Targeting Tumor-Associated Receptors

  • Ayoub Lafnoune,
  • Saad Harrizi,
  • Jihane Ait Benbella,
  • Asmaa Chbel and ,
  • Hicham Wahnou,
  • Ismail Guenaou,
  • Bouchra Darkaoui and
  • Imane Nait Irahal
  • + 3 authors+ 0 author

Tumor angiogenesis plays a critical role in cancer growth and metastatic progression and therefore represents an important therapeutic target. Bee venom-derived peptides constitute a diverse source of bioactive molecules whose interactions with angio...

  • Systematic Review
  • Open Access
537 Views
15 Pages

Virus-like particles (VLPs) are nanoscale platforms that can be engineered for heterologous molecule display. Among the available VLP platforms, Macrobrachium rosenbergii nodavirus-derived VLPs (MrNV VLPs) have emerged as a promising system for this...

  • Article
  • Open Access
885 Views
16 Pages

Pure Mycelium Sheets from Edible Mushrooms for Sustainable Packaging: A Comparative Assessment of Glycerol and ZnONPs Treatment

  • Sasikan Channgam,
  • Gad Elsayed Mohamed Salem,
  • Thatsanee Luangharn,
  • Supachai Pisuchpen and ,
  • Thomas Karbowiak,
  • Pornchai Rachtanapun and
  • Wirongrong Tongdeesoontorn
  • + 2 authors+ 0 author

Increasingly, mycelium-based biomaterials are being investigated as sustainable alternatives to conventional packaging materials, but their inherent brittleness limits their practical application. The present study investigated the properties of pure...

  • Review
  • Open Access
2 Citations
1,025 Views
46 Pages

Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used for the management of inflammatory disorders, pain, and fever due to their significant anti-inflammatory and antipyretic properties. However, their long-term use is often associated with...

(This article belongs to the Special Issue Plant Natural Compounds: From Discovery to Application (2nd Edition))
  • Article
  • Open Access
427 Views
14 Pages

Neurobehavioural Properties of Lima Bean: Antipsychotic, Anxiolytic, and Sedative–Hypnotic-like Actions in Mice

  • Omorovbiye C. Odeh,
  • Emmar E. Okpakpor,
  • Samuel Frank,
  • Israel O. Bolanle and
  • Raymond I. Ozolua

Psychotic disorder remains a major global health burden despite the plethora of anti-psychotic medications currently available. Poor adherence owing to numerous adverse effects and cost burden is a major challenge to treating psychosis. In view of th...

(This article belongs to the Special Issue Functional Food: Molecular Nutrition, Emerging Technologies and Applications)
  • Systematic Review
  • Open Access
658 Views
15 Pages

Effect of Dentin Pretreatment Strategies on Bond Strength to Natural Caries-Affected Dentin: A Systematic Review

  • Rosário Costa,
  • Ana C. Silva,
  • Ana Portela,
  • João C. Ferreira and ,
  • Patrícia T. Pires and
  • Paulo Melo
  • + 1 author+ 0 author

Background: This systematic review aimed to evaluate the effect of dentin pretreatment strategies on bond strength to natural caries-affected dentin (NCAD) in permanent human teeth. Methods: A systematic search was conducted in PubMed, Web of Science...

  • Review
  • Open Access
1 Citations
2,047 Views
34 Pages

Skin aging is a multifactorial process involving structural and functional changes in the dermis. In recent years, the treatment of age-related skin changes in the face, neck, and décolleté has become a growing focus in the fields of es...

  • Article
  • Open Access
1 Citations
769 Views
21 Pages

Plastome Super-Barcoding Readiness of the Flora of Poland: Coverage, Provenance, and the Resolution Gap

  • Monika Szczecińska,
  • Aniela Garbowska,
  • Natan Pupek,
  • Wiktoria Czochór and ,
  • Kamil Koczwara,
  • Joanna Szablińska and
  • Jakub Sawicki
  • + 2 authors+ 0 author

Reliable molecular identification of plants depends on reference libraries of DNA sequences, and complete plastid genomes (plastomes) used as super-barcodes are increasingly the reference of choice for taxonomically difficult groups. We assess how re...

  • Article
  • Open Access
802 Views
36 Pages

From Geometric Complexity to Informational Dimensionality in Scaffold-Guided Tissue Regeneration

  • Maria Teresa Colangelo,
  • Marco Meleti,
  • Stefano Guizzardi and
  • Carlo Galli

Scaffold architecture shapes tissue regeneration through the mechanical, topographical, and biochemical cues it presents to cells, yet geometrically elaborate scaffolds do not reliably produce more organized tissues, while comparatively simple archit...

(This article belongs to the Special Issue Anatomy and Regenerative Medicine: From Methods to Applications (2nd Edition))
  • Review
  • Open Access
1,105 Views
36 Pages

Curcumin-Based Nanocarriers for Alzheimer’s Disease: A Promising Therapeutic Avenue

  • Wushan T. Jayalath,
  • Medha J. Gunaratna,
  • Chamila C. Kadigamuwa,
  • Charitha Thambiliyagodage and
  • Jayangika N. Dahanayake

Globally, over 55 million people are affected by Alzheimer’s disease (AD), a number that is increasing rapidly and projected to triple by 2050. AD is a type of neurodegenerative disorder for which there is no curative treatment. The accumulatio...

  • Article
  • Open Access
935 Views
16 Pages

Microbial Diversity and Antifungal Potential of Lactic Acid and Acetic Acid Bacteria from Vietnamese Fermented Cocoa Beans

  • Tien Cuong Nguyen,
  • Thuy Hang Dam,
  • Thi Thao Hoang,
  • Thi Hong Van Nguyen and ,
  • Hai Van Nguyen,
  • Phu Ha Ho and
  • Hong Nga Luong
  • + 2 authors+ 0 author

This study explored the microbial diversity of Vietnamese cocoa fermentation and the antifungal potential of indigenous bacteria. Cocoa beans were collected from three Vietnamese regions at different fermentation stages. Bacteria were isolated and id...

  • Article
  • Open Access
875 Views
18 Pages

Improvement of Functional Properties and In Vitro Digestibility of Durum Wheat, Chickpea, and Amaranth Flours Using Power Ultrasound

  • Blanca Aurora Francisco-Ponce,
  • Yanik Ixchel Maldonado-Astudillo,
  • Iris Paola Guzmán-Guzmán,
  • Gerardo Huerta-Beristain and ,
  • Gerónimo Arámbula-Villa,
  • Verónica Flores-Casamayor,
  • José Juan Véles-Medina,
  • Patricia Alvarez-Fitz,
  • Mónica Ramírez and
  • Javier Jiménez-Hernández
  • + 7 authors+ 2 authors

Ultrasound technology has emerged as a promising non-thermal approach for modifying the structural and functional properties of food matrices. However, its impact on starch digestibility remains insufficiently understood, particularly in complex syst...

  • Review
  • Open Access
1 Citations
461 Views
28 Pages

Reinforcement Learning for Ultrasound Image Analysis: A Scoping Review

  • Maha Ezzelarab,
  • Midhila Madhusoodanan,
  • Shrimanti Ghosh,
  • Geetika Vadali and ,
  • Jacob L. Jaremko and
  • Abhilash Hareendranathan
  • + 1 author+ 0 author

Machine learning using supervised approaches has been widely applied to ultrasound image analysis. In contrast, reinforcement learning (RL), which is well suited for sequential decision-making, is underexplored. Ultrasound workflows involve sequentia...

  • Article
  • Open Access
518 Views
16 Pages

Sustainable Valorization of Sinami Fruit (Oenocarpus mapora H. Karst), with Focus on Its Nutrients and Bioactive Components as a Function of Harvest Time

  • Winy Cuchuyrumi-Nina,
  • Eliana Molina Mamani,
  • María Isabel Cajo-Pinche,
  • Javier Diaz-Viteri and ,
  • Ana María Muñoz and
  • Fernando Ramos-Escudero
  • + 1 author+ 0 author

Sinami (Oenocarpus mapora H. Karst) is a palm tree that grows in the South American Amazon, whose fruits are highly valued for their sensory characteristics, attractive dark purple color, and as a source of edible oil. However, little to no informati...

(This article belongs to the Special Issue Plant Natural Compounds: From Discovery to Application (2nd Edition))
  • Article
  • Open Access
1 Citations
628 Views
20 Pages

Estrogen-Responsive Gene Modulation by Mentha pulegium L. Extract in Uterine and Ovarian Tissues of Immature Rat

  • Lorraine Sallah,
  • Patrick W. Narkwa,
  • Seth A. Domfeh,
  • Peter N. Coffie and ,
  • Patience N. Ansong,
  • Cynthia A. Danquah,
  • Kofi O. Owusu-Daaku and
  • Babatunde M. Duduyemi
  • + 3 authors+ 0 author

Mentha pulegium L. is reported to contain phytochemicals known to bind to estrogen receptors and modulate estrogenic effects. A hydroethanolic leaf extract of Mentha pulegium L. (MPE) was prepared, and its effects on uterine and ovarian tissues in im...

(This article belongs to the Special Issue Plant Natural Compounds: From Discovery to Application (2nd Edition))
  • Article
  • Open Access
523 Views
11 Pages

Metabolic Fingerprints of Compost and Frass by 1H-HRMAS NMR: Detection of Betaine-Related Biotransformation Products

  • Rubén Gil-Gonzalo,
  • Palmira Villa-Valverde,
  • Inmaculada Aranaz and
  • David Castejón

In the valorization of organic waste within circular bioeconomy frameworks, compost and insect frass are increasingly used as soil amendments, yet their molecular composition and the metabolic signatures of biological processing remain poorly charact...

  • Review
  • Open Access
1 Citations
946 Views
30 Pages

Salivary Calcium as a Biomarker: Analytical Challenges and Clinical Interpretation—A Narrative Review

  • Ivana Šutej,
  • Krešimir Bašić,
  • Matej Kurkutović,
  • Kaltrina Smajli Vokshi and ,
  • Jasmina Šipkar,
  • Karla Sučević and
  • Kristina Peroš
  • + 2 authors+ 0 author

Salivary calcium is a biologically plausible but methodologically challenging biomarker because of its central role in enamel demineralization/remineralization, dental calculus formation, and oral mineral homeostasis. This narrative review summarizes...

  • Article
  • Open Access
823 Views
17 Pages

Hydroxyapatite–Magnesium Bioceramics: Synthesis and Mechanical–Chemical Characterization

  • Elizabeth Refugio-García,
  • Zaira Itzel Bedolla-Valdez,
  • Alfredo Emiliano Chávez-Pantiga,
  • Gerardo Vázquez-Huerta and ,
  • José Guadalupe Miranda-Hernández,
  • Carlos Adrián Calles-Arriaga,
  • José Amparo Rodríguez-García and
  • Enrique Rocha-Rangel
  • + 3 authors+ 0 author

Hydroxyapatite (HA) is widely used in biomedical applications due to its biocompatibility and chemical similarity to the mineral phase of bone; however, its low mechanical strength limits its structural use. In this work, HA ceramics with different M...

(This article belongs to the Topic Advances in Biomaterials—2nd Edition)
  • Article
  • Open Access
728 Views
31 Pages

Carbohydrate Availability Modulates Biomass, Architecture, and Viability of Mono- and Polymicrobial ESKAPE Biofilms

  • Majd M. Alsaleh,
  • Dana A. Alqudah,
  • Bassam I. El-Eswed and
  • Mamoon M. D. Al-Rshaidat

Biofilm formation of ESKAPE pathogens creates a major problem in healthcare-associated infections, and nutrient supply significantly contributes to biofilm structure and bacterial survival. An understanding of how various nutritional environments inf...

  • Article
  • Open Access
1,490 Views
23 Pages

Insights into the Ultrastructure of Native Royal Jelly in Complex with Sr-Doped Phosphate Vitreous Powder

  • Sara Botezan,
  • Răzvan Ștefan,
  • Lucian Barbu-Tudoran,
  • Septimiu Cassian Tripon and ,
  • Marin Șenilă,
  • Victorița Bonta and
  • Daniel Severus Dezmirean
  • + 2 authors+ 0 author

Royal jelly (RJ) is a nutrient-rich, bioactive substance that has been used for medicinal purposes since antiquity. For honeybees, RJ plays a nutritional role and provides structural support for queen larvae in their upside-down oriented hive cells....

  • Article
  • Open Access
1,429 Views
21 Pages

An In Silico and In Vitro Approach to Identify Potential Phytocompounds as Inhibitors of Pneumolysin, a Secretory Toxin of Streptococcus pneumoniae

  • Somya Sephalika,
  • Sanjoy Majumder,
  • Animesh Pattnaik,
  • Arpita Arsmika Sahu and ,
  • Nirmal Kumar Mohakud and
  • Bikash Ranjan Sahu
  • + 1 author+ 0 author

Pneumolysin (PLY), a cholesterol-dependent cytolysin produced by Streptococcus pneumoniae, plays a key role in the pathogenesis of bacterial pneumonia. The present study aimed to identify potential phytocompound inhibitors of pneumolysin and evaluate...

  • Article
  • Open Access
868 Views
17 Pages

Potato (Solanum tuberosum L.) is a vital food security and cash crop in Eritrea, yet bacterial and fungal pathogens responsible for 15–30% yield losses remain molecularly uncharacterized across its production systems. Here, we present the first...

  • Article
  • Open Access
683 Views
16 Pages

Occurrence of Gnomoniopsis smithogilvyi in Chestnut Under Different Management Systems in Northeastern Portugal

  • Sara Reis,
  • Valentim Coelho,
  • Toufiq Yussif,
  • Rosalina Pereira and ,
  • Andreia Tomás and
  • Eugénia Gouveia
  • + 1 author+ 0 author

Chestnut brown rot caused by Gnomoniopsis smithogilvyi represents an increasing threat to Castanea sativa production, although its endophytic behaviour and response to pre-harvest management strategies under field conditions remain poorly understood....

  • Article
  • Open Access
764 Views
16 Pages

Dietary β-Glucan Supplementation Enhances Somatotropic Axis Activity, Growth Performance, and Breast Muscle Meat Quality in Ross 308 Broiler Chickens

  • Luckas Obanda Malachy,
  • Betty Schwartz,
  • Natalie Avital-Cohen,
  • Ofer Gover and ,
  • Hadar Bar-Dagan,
  • Shelly Druyan,
  • Joanna Bartman,
  • Asaf Marco,
  • Dekel Tsalik and
  • Israel Rozenboim
  • + 5 authors+ 0 author

The global push to eliminate antibiotic growth promoters in poultry has accelerated the demand for effective natural alternatives. β-Glucans—branched polysaccharides derived from Saccharomyces cerevisiae cell walls—enhance immunity a...

(This article belongs to the Special Issue Experimental Biology: From Methods to Applications—Under the Auspices of the Italian Society of Experimental Biology, SIBS-1925)
  • Article
  • Open Access
1 Citations
1,018 Views
14 Pages

MuCTAB: An Optimized Protocol for High-Molecular-Weight DNA Extraction from Mucilage-Rich Selenicereus Tissues for Long-Read Sequencing

  • Angel David Hernández-Amasifuen,
  • Julio Cesar Santos-Pelaez,
  • Jheyson Yopan-De la Cruz,
  • Jorge Alberto Condori-Apfata and
  • Juan Carlos Guerrero-Abad

Extracting high-molecular-weight (HMW) DNA from cactus tissues remains technically challenging due to the abundance of mucilage, pectins, polyphenols, and other metabolites that compromise DNA purity, increase viscosity, and reduce integrity, thereby...

  • Review
  • Open Access
780 Views
21 Pages

Periodontitis is a chronic inflammatory disease initiated by dysbiotic biofilms and sustained by an altered host response, resulting in progressive destruction of the tooth-supporting tissues. Alongside immune-mediated damage, epithelial–mesenc...

  • Review
  • Open Access
1 Citations
1,078 Views
19 Pages

Biocontrol using antagonistic bacteria is considered a promising and sustainable approach for managing tomato bacterial diseases. Many antagonistic bacterial strains show strong activity under laboratory conditions, but their performance in greenhous...

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Appl. Biosci. - ISSN 2813-0464